Article
Biochemistry & Molecular Biology
Constanca Junior, Anna Ulldemolins, Maria Narciso, Isaac Almendros, Ramon Farre, Daniel Navajas, Javier Lopez, Mar Eroles, Felix Rico, Nuria Gavara
Summary: The extracellular matrix (ECM) of the lung, composed mainly of collagens, elastin, and proteoglycans, provides support to lung cells. Aberrant increased deposition of ECM in lung fibrosis changes filament organization and stiffens the ECM, leading to the profibrotic profile of pulmonary cells. The study examines changes in ECM properties during fibrosis development and identifies potential pharmacological targets to hinder its progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Physical
Chunlei Yang, Tianhui Su, Yanbo Hua, Liming Zhang
Summary: This review summarizes the latest results of electrochemical scanning probe microscopy techniques in studying the interfacial properties of electrocatalysts and photoelectrodes, and offers some perspectives on future research directions.
Review
Chemistry, Physical
Prachi Ghoderao, Sanjay Sahare, Shern-Long Lee, Prashant Sonar
Summary: Scanning probe microscopy (SPM) is a powerful technique that allows for the observation and analysis of nanoscale materials and biological molecules through the use of sharp probes that scan the sample surface. This method, which includes scanning tunneling microscopy (STM) and atomic force microscopy (AFM), provides valuable insights into the properties and behavior of these materials at a molecular level. This review highlights the applications of SPM in biomolecule imaging and the study of physiological processes, as well as the challenges and potential solutions in biological sample preparation. It also discusses the recent advancements in STM and AFM applications on biomolecules, such as DNA, proteins, and carbohydrates, and their potential applications in fields like drug delivery and biosensors.
Article
Engineering, Biomedical
Yuji Nashimoto, Minori Abe, Ryota Fujii, Noriko Taira, Hiroki Ida, Yasufumi Takahashi, Kosuke Ino, Javier Ramon-Azcon, Hitoshi Shiku
Summary: This study evaluates the role of scanning probe microscopy (SPM) as an analytical tool for microphysiological systems (MPS), providing a new solution for real-time high spatial resolution imaging. A simple vascular model was used for permeability and topographical analysis, paving the way for the application of SPM technology in MPS.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Kohei Yamasue, Yasuo Cho
Summary: Scanning nonlinear dielectric microscopy (SNDM) is a near-field microwave-based scanning probe microscopy method that has wide applications. It is often combined with contact-mode atomic force microscopy (AFM) for topography imaging and contact force regulation. However, SNDM with intermittent contact AFM may result in a lower signal-to-noise ratio. To improve this, we propose a boxcar averaging based signal acquisition method and demonstrate its enhancement on SNDM combined with peak-force tapping AFM. The proposed method is also applicable to other scanning near-field microwave microscopes.
Review
Chemistry, Multidisciplinary
David Necas, Petr Klapetek
Summary: Synthetic data play an increasingly important role in nanometrology for development of data processing methods and analysis of uncertainties. This paper reviews methods for generating synthetic data and their applications in scanning probe microscopy, focusing on principles, performance, and applicability. The benefits of using synthetic data in tasks related to improving scanning approaches and estimating reliability of data processing methods are demonstrated, along with analyzing systematic errors common to scanning probe microscopy methods.
Article
Chemistry, Multidisciplinary
Nicolas Neel, Joerg Kroeger
Summary: The study revealed that the formation of a chemical bond between a CO molecule and a Cu(111) surface is accompanied by bending of the molecular probe due to van der Waals attraction and Pauli repulsion, resulting in characteristic changes in the vertical force between CO and Cu(111) as a function of molecule-surface distance. Experimental data were successfully reproduced by model calculations, shedding new light on vertical-force extrema in scanning probe experiments.
Article
Chemistry, Analytical
Pengfei Fan, Jian Gao, Hui Mao, Yanquan Geng, Yongda Yan, Yuzhang Wang, Saurav Goel, Xichun Luo
Summary: This article presents the mechanism, current research, and application characteristics of scanning probe lithography (SPL) nanofabrication technique, and analyzes the effects of thermal, chemical, electric, and magnetic fields on this technology. The article also compares different fabrication capabilities, throughput, and attainable resolution, and points out that SPL has yet to achieve its full commercial potential.
Article
Biochemistry & Molecular Biology
Tong Zhu, Jingjing Song, Bin Gao, Junjie Zhang, Yabei Li, Zhaoyang Ye, Yuxiang Zhao, Xiaogang Guo, Feng Xu, Fei Li
Summary: In this study, the effect of extracellular matrix (ECM) stiffness on the efficacy of candesartan (CAN) in anti-fibrosis and antioxidant treatment was investigated. The results showed that CAN had the best improvement efficacy in rats with medium-stiffness myocardial tissue. Cell culture substrates mimicking the ECM stiffness of cardiac fibroblasts (CFs) at different stages of myocardial fibrosis were used to study the effects of ECM stiffness on CFs, and it was found that CAN had the most significant anti-fibrosis and antioxidant efficacy on medium-stiffness substrates.
Article
Chemistry, Multidisciplinary
Mahmoud Behzadirad, Stephan Mecholdt, John N. Randall, Joshua B. Ballard, James Owen, Ashwin K. Rishinaramangalam, Alexander Reum, Teodor Gotszalk, Daniel F. Feezell, Ivo W. Rangelow, Tito Busani
Summary: This article introduces the use of GaN nanowires as materials for scanning probe lithography and microscopy, with a low-cost fabrication process and the ability to provide sub-10 nm lithography and atomic scale resolution. GaN nanowires perform well in advanced SPL and imaging.
Article
Energy & Fuels
Christian Kameni Boumenou, Amala Elizabeth, Finn Babbe, Alice Debot, Harry Moeenig, Alex Redinger
Summary: The study investigated the buried MoSe2/CuInSe2 interface of stoichiometric absorbers in thin film solar cells, revealing differences in local density of states compared to the front-side properties. This sheds new light on the complex interface formation in CuInSe2-based thin film solar cells grown under Cu-rich conditions.
PROGRESS IN PHOTOVOLTAICS
(2021)
Article
Multidisciplinary Sciences
Ayana Sugizaki, Keisuke Sato, Kazuyoshi Chiba, Kenta Saito, Masahiko Kawagishi, Yuri Tomabechi, Shalin B. Mehta, Hirokazu Ishii, Naoki Sakai, Mikako Shirouzu, Tomomi Tani, Sumio Terada
Summary: Biomolecular assemblies play a crucial role in cell physiology, with their function depending on the changes in molecular positions and orientations. Fluorescence polarization microscopy offers a promising solution by reporting the orientation of emission dipoles, providing insights into monitoring molecular orientation in real time within living cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Chi Zhang, Jason M. Bartell, Jonathan C. Karsch, Isaiah Gray, Gregory D. Fuchs
Summary: A time-resolved near-field magnetic microscope based on magnetothermal interactions is developed, providing spatial resolution on the scale of 100 nm and a temporal resolution below 100 ps. This microscope offers an accessible and comparatively low-cost approach for nanoscale spatiotemporal magnetic microscopy in laboratories.
Article
Cell Biology
Sandeep M. Nalluri, Chinmay S. Sankhe, Joseph W. O'Connor, Paul L. Blanchard, Joelle N. Khouri, Steven H. Phan, Gage Virgi, Esther W. Gomez
Summary: Epithelial-mesenchymal transition (EMT) is a physiological process essential for embryogenesis and wound healing, but also implicated in pathologies like fibrosis and cancer. Regulation of phosphorylation status can impact cell physiology and gene expression changes during TGFβ1-induced EMT, highlighting the crucial role of intricate crosstalk between signaling cascades in regulating EMT processes.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Qianru Mei, Zhe Liu, He Zuo, Zhenhua Yang, Jing Qu
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal lung disease with limited understanding of its pathogenesis. Recent studies suggest that sustained lung epithelial injury and fibroblast differentiation play key roles in the development of IPF. Current treatments can slow disease progression but cannot cure it.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Frederic Tewes, Oliviero L. Gobbo, Carsten Ehrhardt, Anne Marie Healy
ACS APPLIED MATERIALS & INTERFACES
(2016)
Review
Chemistry, Multidisciplinary
Alexander N. Zelikin, Carsten Ehrhardt, Anne Marie Healy
Article
Pharmacology & Pharmacy
Xiao Hu, Fei-Fei Yang, Chun-Yu Liu, Carsten Ehrhardt, Yong-Hong Liao
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2017)
Article
Chemistry, Medicinal
Carsten Ehrhardt, Per Backman, William Couet, Chris Edwards, Ben Forbes, Markus Friden, Mark Gumbleton, Ken-Ichi Hosoya, Yukio Kato, Takeo Nakanishi, Mikihisa Takano, Tetsuya Terasaki, Ryoko Yumoto
JOURNAL OF PHARMACEUTICAL SCIENCES
(2017)
Article
Chemistry, Multidisciplinary
Sabrina Nickel, Mohammed Ali Selo, Juliane Fallack, Caoimhe G. Clerkin, Hanno Huwer, Nicole Schneider-Daum, Claus-Michael Lehr, Carsten Ehrhardt
PHARMACEUTICAL RESEARCH
(2017)
Editorial Material
Chemistry, Multidisciplinary
Carsten Ehrhardt
PHARMACEUTICAL RESEARCH
(2017)
Article
Pharmacology & Pharmacy
Amani Alhayali, Mohammed Ali Selo, Carsten Ehrhardt, Sitaram Velaga
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2018)
Article
Pharmacology & Pharmacy
Lili Duse, Michael Rene Agel, Shashank Reddy Pinnapireddy, Jens Schafer, Mohammed A. Selo, Carsten Ehrhardt, Udo Bakowsky
Article
Pharmacology & Pharmacy
Johanna J. Salomon, Julia C. Gausterer, Mohammed Ali Selo, Ken-ichi Hosoya, Hanno Huwer, Nicole Schneider-Daum, Claus-Michael Lehr, Carsten Ehrhardt
Editorial Material
Pharmacology & Pharmacy
Ayca Yildiz-Pekoz, Carsten Ehrhardt
Review
Biochemistry & Molecular Biology
Mohammed Ali Selo, Johannes A. Sake, Carsten Ehrhardt, Johanna J. Salomon
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Chemistry, Applied
Julia Cedran Coco, Janaina Artem Ataide, Johannes A. Sake, Elias Basile Tambourgi, Carsten Ehrhardt, Priscila Gava Mazzola
Summary: The research demonstrated that baru nut extract has strong antioxidant activity and wound healing properties, showing potential benefits in alleviating diseases related to oxidative stress and impaired wound healing.
NATURAL PRODUCT RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Severin Mairinger, Irene Hernandez-Lozano, Markus Zeitlinger, Carsten Ehrhardt, Oliver Langer
Summary: This review discusses the potential of nuclear medicine imaging techniques in inhalation biopharmaceutical research, including the evaluation of pulmonary drug deposition, assessment of intrapulmonary pharmacokinetics, and quantification of the pharmacodynamic effect of inhaled drugs. Despite the limited use of imaging techniques in pulmonary drug development, they have the potential to bridge the knowledge gap between animal models and humans with respect to the pulmonary disposition of inhaled drugs.
EXPERT OPINION ON DRUG DELIVERY
(2022)
Article
Pharmacology & Pharmacy
Johannes A. Sake, Mohammed Ali Selo, Lyubomyr Burtnyak, Henriette E. Dahnhardt, Camelia Helbet, Severin Mairinger, Oliver Langer, Vincent P. Kelly, Carsten Ehrhardt
Summary: Multidrug resistance-associated protein 1 (MRP1/ABCC1) is an efflux transporter responsible for the extrusion of endogenous substances as well as xenobiotics and their respective metabolites. The generation of an ABCC1-/- clone based on the human distal lung epithelial cell line NCI-H441 via a targeted CRISPR/Cas9 approach provides a useful tool to study MRP1 function in vitro. The ABCC1-/- NCI-H441 clone showed complete absence of mRNA and protein, and retained the ability to form electrically tight barriers.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Wilbur de Kruijf, Carsten Ehrhardt
CURRENT OPINION IN PHARMACOLOGY
(2017)
Article
Nanoscience & Nanotechnology
Daniela Rubanova, Svitlana Skoroplyas, Alena Libanska, Eva Randarova, Josef Bryja, Michaela Chorvatova, Tomas Etrych, Lukas Kubala
Summary: This study explored the use of nanocarriers to deliver dexamethasone for treating rheumatoid arthritis. The results showed that this method increased the accumulation of dexamethasone in the arthritic area, had a dose-dependent healing effect, and reduced the accumulation of the RA mediator RANKL. This study provides a feasible strategy for improving rheumatoid arthritis therapy.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)
Article
Nanoscience & Nanotechnology
Thanpisit Lomphithak, Apiwit Sae-Fung, Simone Sprio, Anna Tampieri, Siriporn Jitkaew, Bengt Fadeel
Summary: In this study, iron-doped hydroxyapatite (FeHA) was evaluated as a potential nanomedicine approach for treating PDAC. The results showed that FeHA, in combination with a sublethal dose of the GPX4 inhibitor RSL3, could trigger ferroptosis in KRAS mutant PANC-1 cells while sparing normal human cells. The induction of ferroptosis by FeHA plus RSL3 was found to be dependent on the metalloreductase STEAP3 in PDAC cells.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)
Review
Nanoscience & Nanotechnology
Marco Felipe Salas-Orozco, Ana Cecilia Lorenzo-Leal, Idania de Alba Montero, Nuria Patino Marin, Miguel Angel Casillas Santana, Horacio Bach
Summary: The emergence of antibiotic-resistant bacteria in severe infections is a growing concern, particularly in hospital environments. Metal-based nanomaterials have shown potential as an alternative to combat these bacteria, but there is a risk of bacterial resistance and environmental accumulation.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)
