Article
Chemistry, Multidisciplinary
Ji Liu, Jinhan Sheng, Leihou Shao, Qizhen Zheng, Wenting Li, Xianghan Chen, Lanqun Mao, Ming Wang
Summary: Hierarchical self-assembly of TPE-featured metal-organic cages and beta-cyclodextrin-conjugated polyethylenimine generates fluorescent supramolecular nanoparticles (FSNPs) for efficient protein delivery into neural cells, allowing rapid release into the cytosol for subcellular targeting. Delivery of molecular chaperone DJ-1 using FSNPs activates MAPK/ERK signaling in neural cells to protect cells from oxidative stress.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Chemistry, Multidisciplinary
Jeongsoo Hur, Aram J. Chung
Summary: This review provides an overview of recent advances in microfluidic and nanofluidic intracellular delivery strategies and discusses new opportunities and challenges for clinical applications. Microfluidic and nanofluidic approaches as emerging solutions have shown remarkable potential for addressing this open challenge.
Article
Chemistry, Multidisciplinary
Sibei Lei, Xiaohua Chen, Yan Gao, Ming Shuai, Weilin Zhou, Jingmei Li, Jieping Wu, Ke Men, Xingmei Duan
Summary: mRNA-based gene therapy shows promise for treating hepatocellular carcinoma (HCC), but lacks precision in vivo. This study develops a novel HCC-specific peptide, HCC167, which can specifically recognize HCC cells and facilitate active targeting. When loaded with Bims-encoding mRNA, the HCC167-modified nanoparticles effectively suppress HCC progression in multiple models. The study also identifies the ALPPL2 protein as the specific binding receptor of the HCC167 peptide. This research demonstrates a potent active targeting strategy for mRNA-based HCC therapy.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Engineering, Biomedical
Yu Xia, Guoyi Tang, Yi Chen, Changbing Wang, Min Guo, Tiantian Xu, Mingqi Zhao, Yongjian Zhou
Summary: RNA interference (RNAi) is a promising method for treating malignant tumors, and in this study, RGDfC-SeNPs were successfully synthesized as a tumor-targeted carrier for delivering siSox2 to HepG2 liver cancer cells. The results showed that RGDfC-Se@siSox2 effectively silenced Sox2, inhibited tumor cell proliferation, migration, and invasion, induced apoptosis through reactive oxygen species production and mitochondrial membrane potential disruption, and significantly inhibited tumor growth in mice bearing HepG2 tumors. These findings suggest that RGDfC-SeNPs could be a valuable gene carrier for specific gene-targeted therapy in HCC.
BIOACTIVE MATERIALS
(2021)
Review
Pharmacology & Pharmacy
Yu Wan, Wangxia Wang, Qiuyue Lai, Mingyu Wu, Shun Feng
Summary: Efficient intracellular delivery is crucial but faces a dilemma between efficiency and toxicity. Cell-penetrating poly(disulfide) (CPD) is an effective tool for intracellular delivery, avoiding endolysosomal trapping and ensuring cytosolic availability. CPD undergoes reductive depolymerization inside cells and has minimal cytotoxicity. This review summarizes CPD's synthesis approaches, cellular uptake mechanism, and recent advances in intracellular delivery of various substances. CPD shows promise as a carrier for efficient intracellular delivery.
DRUG DISCOVERY TODAY
(2023)
Review
Polymer Science
Yuhe Zhang, Jiahao Shi, Bin Ma, Ya-Nan Zhou, Haiyang Yong, Jianzhong Li, Xiangyi Kong, Dezhong Zhou
Summary: Protein drugs have gained popularity due to their high activity, specificity, and low cytotoxicity, but face limitations in terms of structural stability, immune clearance, and cell penetration. Functionalizing polymers can enhance the delivery of protein drugs by improving stability, immune evasion, and cellular uptake.
PROGRESS IN POLYMER SCIENCE
(2023)
Review
Pharmacology & Pharmacy
Yamin Li, Zhongfeng Ye, Hanyi Yang, Qiaobing Xu
Summary: Lipid nanoparticle (LNP) -based drug delivery systems hold great potential in clinical applications and can encapsulate and transport various bioactive substances. To ensure the safe and efficient delivery of cargo molecules, careful design of each LNP carrier system is necessary. Our research group has developed novel LNP delivery systems through combinatorial library synthesis and in vitro and in vivo screening, and evaluated and optimized their drug delivery effects.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Chemistry, Multidisciplinary
Echua Tan, Tao Wan, Chunlei Yu, Qianqian Fan, Wenbang Liu, Hong Chang, Jia Lv, Hui Wang, Dali Li, Yuan Ping, Yiyun Cheng
Summary: Protein therapeutics have become a rapidly growing class of medicine in recent years due to their high potency and specificity. However, delivering protein drugs to intracellular targets is currently not possible. Therefore, there is a pressing need to develop efficient carriers for intracellular protein delivery. This study reports the development of a reactive oxygen species responsive material based on biodegradable polypeptide, which shows promising results for cytosolic protein delivery.
Article
Oncology
Mohd Yasser, Silvia Ribback, Katja Evert, Kirsten Utpatel, Katharina Annweiler, Matthias Evert, Frank Dombrowski, Diego F. F. Calvisi
Summary: Our electron microscopic findings suggest that plasmid transfer to hepatocytes may occur through active or passive endocytosis, with plasmids being trapped in membrane-bound vesicles and vacuoles. We observed vacuole formation in the proximity of the nucleus, which is believed to be the site of DNA-entry. However, the exact mechanisms by which plasmids are incorporated into hepatocytes and how vesicle generation is involved remain unclear.
Article
Chemistry, Multidisciplinary
Yishu Bao, Hongfei Chen, Zhiyi Xu, Jiayang Gao, Liwen Jiang, Jiang Xia
Summary: The article describes a new fluorescent molecule that can deliver proteins into mammalian cells and release the payload into the cytosol through photoinduced disintegration.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Gastroenterology & Hepatology
Lorenza Rimassa, Richard S. Finn, Bruno Sangro
Summary: Single-agent immune checkpoint inhibitors (ICIs) have limited efficacy in advanced hepatocellular carcinoma (HCC) patients, and approximately 30% of patients show intrinsic resistance to ICIs. Therefore, researchers are exploring combination therapies, such as anti-angiogenic agents and other ICIs. Some trials have shown promising results and potential for clinical application. However, there are still many unanswered questions that require further research.
JOURNAL OF HEPATOLOGY
(2023)
Review
Pharmacology & Pharmacy
Shijing Yu, Ruirui Zhao, Bingchen Zhang, Chunmei Lai, Linyan Li, Jiangwen Shen, Xiarong Tan, Jingwei Shao
Summary: Hepatocellular carcinoma (HCC) is a common cause of cancer death, and patient survival has not improved significantly. Traditional therapies like surgery, chemotherapy, and radiotherapy have achieved good results, but challenges such as drug resistance and toxicity still exist. CRISPR/Cas9 gene therapy has emerged as an alternative to traditional treatments, attracting attention for its ability to eliminate resistant tumors and edit target genes.
ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yue Gao, Waicong Dai, Zhijun Ouyang, Mingwu Shen, Xiangyang Shi
Summary: This study investigated the intracellular delivery of fibronectin (FN) using phenylboronic acid (PBA)-functionalized generation 5 (G5) poly(amidoamine) dendrimers (G5.NH2-PBA) as nanocarriers. It was found that this method effectively guided macrophages towards anti-inflammatory M2 polarization. Mechanistic studies revealed that the dendrimer-mediated FN delivery suppressed the nuclear factor-κB pathway, resulting in reduced pro-inflammatory cytokine secretion and enhanced reactive oxygen species depletion. Furthermore, in vivo experiments demonstrated the potential of dendrimer-mediated FN delivery in alleviating lung inflammation and oxidative stress in acute lung injury.
Article
Neurosciences
Kaitlyn M. Roman, Maria A. Briscione, Yuping Donsante, Jordan Ingram, Xueliang Fan, Douglas Bernhard, Simone A. Campbell, Anthony M. Downs, David Gutman, Tejas A. Sardar, Sofia Q. Bonno, Diane J. Sutcliffe, H. A. Jinnah, Ellen J. Hess
Summary: The mechanisms underlying dystonia are largely unknown, but it is often associated with abnormal dopamine neurotransmission. This study aimed to understand dopaminergic adaptations in dystonia by investigating the intracellular signaling pathways mediated by dopamine receptors in a knockin mouse model of DOPA-responsive dystonia (DRD). The results showed that L-DOPA treatment induced the phosphorylation of protein kinase A and ERK, primarily in D1 dopamine receptor-expressing striatal neurons. The dysregulated signaling varied across different striatal subdomains, with ERK phosphorylation predominantly occurring in the dorsomedial (associative) striatum and little response in the dorsolateral (sensorimotor) striatum. This regional variation in dopamine-mediated neurotransmission may be a hallmark of dystonia and distinguishes it from other dopamine deficiency disorders like Parkinson's disease.
Article
Chemistry, Multidisciplinary
Dominik Witzigmann, Philip Grossen, Cristina Quintavalle, Manuela Lanzafame, Susanne H. Schenk, Xue-Ting Tran, Bernhard Englinger, Patrick Hauswirth, David Gruenig, Sushilla van Schoonhoven, Stephan Krahenbuhl, Luigi M. Terracciano, Walter Berger, Salvatore Piscuoglio, Luca Quagliata, Jean Rommelaere, Juerg P. F. Nueesch, Jorg Huwyler
Summary: This study investigated a nonviral gene therapy approach targeting HCC with the NS1 protein, identifying PDK1 as a potential predictive biomarker for NS1-LPX gene therapy effectiveness. Experimental results demonstrated that the NS1-LPX therapeutic approach significantly reduced tumor growth and prolonged survival in an HCC xenograft mouse model.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Engineering, Civil
Taek-Jin Song, Jongoh Jeong, Jong-Hwan Kim
Summary: Semantic segmentation and depth estimation are crucial for scene understanding and autonomous driving. The proposed RODSNet is able to recognize unexpected small obstacles on the road by simultaneously refining semantic segmentation and disparity maps from a stereo RGB pair.
IEEE TRANSACTIONS ON INTELLIGENT TRANSPORTATION SYSTEMS
(2022)
Article
Chemistry, Multidisciplinary
Mai Shinohara, Yuya Ashikaga, Wei Xu, Sunnam Kim, Tuyoshi Fukaminato, Takuro Niidome, Seiji Kurihara
Summary: This study investigated the photochemical switching of cytotoxicity by using spiropyran compounds with pyridinium and alkyl groups. The results showed that the two isomers of SP6 (MC and SP) displayed different cytotoxicity under visible light irradiation at different concentrations, achieving the photochemical switching of cytotoxicity. These findings are important for the development of novel photochemotherapy drugs.
Article
Chemistry, Multidisciplinary
Emi Takeda, Wei Xu, Mitsuhiro Terakawa, Takuro Niidome
Summary: Research showed that triangular silver nanoparticles coated with silica exhibited strong antimicrobial activity under high laser power, but crystallization of the silica layer at moderate power levels led to decreased antimicrobial activity.
Article
Robotics
Ue-Hwan Kim, Se-Ho Kim, Jong-Hwan Kim
Summary: This study proposes the SimVODIS++ architecture, which combines neural semantic visual odometry, object detection, and instance segmentation to improve VO performance in dynamic environments. Experimental results demonstrate the effectiveness of SimVODIS++ and the solution to the data leakage problem in the conventional experiment setting.
IEEE ROBOTICS AND AUTOMATION LETTERS
(2022)
Article
Food Science & Technology
Jae-Han Shim, Md Musfiqur Rahman, Ahmed A. Zaky, Shin-Jee Lee, Ara Jo, Seung-Hee Yun, Jong-Bang Eun, Jong-Hwan Kim, Jong-Woo Park, Emel Oz, Charalampos Proestos, Fatih Oz, A. M. Abd El-Aty
Summary: An analytical method for the simultaneous determination of pyridate, quizalofop-ethyl, and cyhalofop-butyl in agricultural products was developed using LC-MS/MS. The method utilized optimized purification with Z-SEP sorbent and achieved good linearity, with a determination coefficient of >= 0.999. The method was validated and showed acceptable recovery and precision. Real-world market samples tested negative for the herbicide residues, demonstrating the applicability of the developed method for routine analysis.
Article
Engineering, Biomedical
Riki Toita, Jeong-Hun Kang, Akira Tsuchiya
Summary: This study developed a novel strategy using artificial apoptotic cell mimetics (phosphatidylserine liposomes; PSLs) to promote tissue repair and tissue-to-biomaterial integration by inducing M1-to-M2 macrophage transition, with bone as the model tissue. The results showed that PSL-multilayer-coated implants enhanced de novo bone formation and bone-to-implant integration, triggering the proper generation and activation of bone-forming osteoblasts and bone-resorbing osteoclasts. These findings demonstrate the potential of PSL-multilayers in bone regeneration and have significant implications for the design of new immunomodulatory biomaterials.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Multidisciplinary
Yuko Sato, Daisuke Asa, Keiko Terada, Jeong-Hun Kang, Takeshi Mori, Takuro Niidome, Hideki Nakashima, Yoshiki Katayama
Summary: This study demonstrates that by increasing the mobility of DNA strands, the preinitiation complex can better access chromatin-packaged DNA for transcriptional initiation.
Article
Environmental Sciences
Young Sang Kwon, Chang-Beom Park, Seung-Min Lee, Seonggeun Zee, Go-Eun Kim, Yeong-Jin Kim, Hee-Jung Sim, Jong-Hwan Kim, Jong-Su Seo
Summary: This study investigated the protein profiles in zebrafish embryos exposed to BB using mass spectrometry. A total of 83 differentially expressed proteins were identified, and their biological functions were categorized into different functional groups. The research provides insights into the molecular basis of the ecotoxicity of BB in aquatic ecosystems.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Da Hee Hong, Jae Hoon Yoo, Won Ji Park, So Won Kim, Jong Hwan Kim, Sae Hoon Uhm, Hee Chul Lee
Summary: This study investigated the physical and electrical properties of Hf0.5Zr0.5O2 (HZO) thin films deposited by two plasma-enhanced atomic layer deposition (PEALD) methods and the effects of plasma application on the properties of HZO thin films. The results showed that HZO thin films deposited by remote plasma atomic layer deposition (RPALD) exhibited excellent fatigue endurance at a measurement temperature of 60 degrees C or less, while HZO thin films deposited by direct plasma atomic layer deposition (DPALD) quickly deteriorated at higher temperatures. Overall, the RPALD method is promising for the development of ferroelectric memory devices.
Article
Food Science & Technology
Wenting Wang, Jong-Wook Song, Seong-Hoon Jeong, Jung-Hoon Jung, Jong-Su Seo, Jong-Hwan Kim
Summary: The dissipation patterns of four different insecticides on strawberries and the efficiency of household washing methods were investigated. The concentrations of the insecticide residues were below the maximum residue limits (MRLs). Soaking the fruit in a solution and rinsing with running water proved to be more effective in removing residues compared to only soaking. Vinegar and salt solutions were the most efficient in removing residues, while tap water alone had a lower removal efficiency. The estimated risk to human health from consuming strawberries was minimal.
Article
Nanoscience & Nanotechnology
Ayaka Harada, Wei Xu, Katsuhiko Ono, Hiroyasu Tsutsuki, Kinnosuke Yahiro, Tomohiro Sawa, Takuro Niidome
Summary: In this study, the B subunit of subtilase cytotoxin (SubB) was conjugated to silver nanoplates (AgNPLs) for use as an antibacterial drug against intracellularly infecting Salmonella typhimurium (S. typhimurium). The modification of AgNPLs with SubB improved their dispersion stability and antibacterial activity. The SubB modification enhanced the cellular uptake of AgNPLs, leading to the killing of intracellularly infecting S. typhimurium.
ACS APPLIED BIO MATERIALS
(2023)
Article
Engineering, Biomedical
Riki Toita, Masahiro Kitamura, Akira Tsuchiya, Jeong-Hun Kang, Shinjiro Kasahara
Summary: "A new surface engineering strategy is described, which involves the release of phosphatidylserine liposomes (PSLs) from an implant coating to modify macrophage phenotype and mitigate foreign body reactions (FBRs), resulting in improved tissue healing. Unlike other therapeutic approaches, PSL-multilayered coatings also promote muscle regeneration."
ADVANCED HEALTHCARE MATERIALS
(2023)
Proceedings Paper
Computer Science, Artificial Intelligence
Sun-Kyung Lee, Hyun Myung, Jong-Hwan Kim
Summary: The advent of deep learning has changed the trends of various research areas including robotics, particularly in the field of computer vision where deep learning-based grasp pose detection algorithms have been developed. These algorithms have the advantage of generalizing well to unknown environments compared to traditional algorithms. However, computation time still remains an issue due to the common pipeline of sampling and ranking grasp candidates. To address this problem, a lightweight network called GG-CNN with moderate performance has been recently developed. In order to further enhance performance by utilizing multi-modality and the hierarchy of grasp components, we propose a multi-modal hierarchical generative grasping CNN (MMH-GGCNN) with a small number of parameters. Experimental results show that MMH-GGCNN achieves an improved accuracy of 91.9679% on the Cornell Grasping Dataset.
ROBOT INTELLIGENCE TECHNOLOGY AND APPLICATIONS 6
(2022)
Proceedings Paper
Computer Science, Artificial Intelligence
In-Ug Yoon, Ue-Hwan Kim, Hyun Myung, Jong-Hwan Kim
Summary: The study introduces a s-DRN model for online incremental clustering, which achieves more stable and accurate clustering performance through the design of a stable activation function and an effective node grouping algorithm.
ROBOT INTELLIGENCE TECHNOLOGY AND APPLICATIONS 6
(2022)
Article
Nanoscience & Nanotechnology
Daniela Rubanova, Svitlana Skoroplyas, Alena Libanska, Eva Randarova, Josef Bryja, Michaela Chorvatova, Tomas Etrych, Lukas Kubala
Summary: This study explored the use of nanocarriers to deliver dexamethasone for treating rheumatoid arthritis. The results showed that this method increased the accumulation of dexamethasone in the arthritic area, had a dose-dependent healing effect, and reduced the accumulation of the RA mediator RANKL. This study provides a feasible strategy for improving rheumatoid arthritis therapy.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)
Article
Nanoscience & Nanotechnology
Thanpisit Lomphithak, Apiwit Sae-Fung, Simone Sprio, Anna Tampieri, Siriporn Jitkaew, Bengt Fadeel
Summary: In this study, iron-doped hydroxyapatite (FeHA) was evaluated as a potential nanomedicine approach for treating PDAC. The results showed that FeHA, in combination with a sublethal dose of the GPX4 inhibitor RSL3, could trigger ferroptosis in KRAS mutant PANC-1 cells while sparing normal human cells. The induction of ferroptosis by FeHA plus RSL3 was found to be dependent on the metalloreductase STEAP3 in PDAC cells.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)
Review
Nanoscience & Nanotechnology
Marco Felipe Salas-Orozco, Ana Cecilia Lorenzo-Leal, Idania de Alba Montero, Nuria Patino Marin, Miguel Angel Casillas Santana, Horacio Bach
Summary: The emergence of antibiotic-resistant bacteria in severe infections is a growing concern, particularly in hospital environments. Metal-based nanomaterials have shown potential as an alternative to combat these bacteria, but there is a risk of bacterial resistance and environmental accumulation.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)