Article
Pharmacology & Pharmacy
Aleph M. S. Souza, Renato C. A. Ribeiro, Gleyse K. L. O. Pinheiro, Francisco I. Pinheiro, Wogenes N. Oliveira, Luanda B. F. C. Souza, Andre L. Silva, Lucas Amaral-Machado, Everton N. Alencar, Guilherme M. Chaves, Eryvaldo S. T. Egito
Summary: The study successfully produced a nail lacquer loaded with Amphotericin B and evaluated its performance, showing good stability, drug release, and antifungal activity. The results suggest that the AmB-loaded nail lacquer has the potential to be a promising treatment for onychomycosis induced by Candida spp.
Article
Microbiology
A. Espinel-Ingroff, M. Sasso, J. Turnidge, M. Arendrup, F. Botterel, N. Bourgeois, B. Bouteille, E. Canton, S. Cassaing, E. Dannaoui, M. Dehais, L. Delhaes, D. Dupont, A. Fekkar, J. Fuller, G. Garcia-Effron, J. Garcia, G. M. Gonzalez, N. P. Govender, H. Guegan, J. Guinea, S. Houze, C. Lass-Florl, T. Pelaez, A. Forastiero, M. Lackner, R. Magobo
Summary: Susceptibility testing for antifungal drugs is crucial in the clinical setting, relying on categorical endpoints and epidemiological cutoff values for different species. Collaborative efforts have led to the development of Etest sensitivity values for various Candida and Aspergillus species, aiding in clinical laboratory testing.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Medicine, General & Internal
Hangxiang Du, Limin Wei, Wenzhe Li, Bixia Huang, Yongan Liu, Xiaofei Ye, Sheng Zhang, Tao Wang, Yizhu Chen, Dechang Chen, Jiao Liu
Summary: This observational study investigated the potential relationship among airway Candida spp. de-colonization, nebulized amphotericin B (NAB) treatment, and ventilator-associated pneumonia (VAP) occurrence in critically ill patients. Results showed that NAB treatment significantly reduced 90-day mortality, contributed to Candida spp. airway de-colonization, and was associated with a decreased risk of P. aeruginosa-related VAP. NAB may be considered as an alternative treatment option for critically ill patients with VAP.
FRONTIERS IN MEDICINE
(2021)
Review
Infectious Diseases
Zhen Hu, Huaying Zhou
Summary: This study found that amphotericin B may decrease the serum level of voriconazole. More data are needed to support the appropriateness of combining amphotericin B and voriconazole.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Immunology
Li Zhang, Jinzhou Xiao, Mingwei Du, Wenzhi Lei, Weiwei Yang, Xiaochun Xue
Summary: Neutropenia is a common complication in the treatment of hematological diseases and the most common predisposing factor for invasion by fungi, such as Candida krusei. Recent studies have shown that C. krusei, a life-threatening pathogen, has developed resistance to amphotericin B (AMB). However, the mechanisms that led to the rapid emergence of this AMB-resistant phenotype are unclear.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Yulong Tan, Su Ma, Ting Ding, Roland Ludwig, Jintae Lee, Jiaman Xu
Summary: Traditional antifungal therapies are insufficient to treat Candida biofilm-related infections. In this study, chitosan nanoparticles loaded with Amphotericin B and functionalized with beta-1,3-glucanase were found to effectively inhibit Candida albicans biofilm growth, disassemble the biofilm matrix, and eradicate biofilms from clinical isolates, suggesting their potential for the treatment of Candida biofilm-related infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Amal M. Soliman, Walaa Abdel-Latif, Iman H. Shehata, Amr Fouda, Abdullah M. Abdo, Yasmin M. Ahmed
Summary: In this study, a green approach was developed to control pathogenic Candida spp. isolated from clinical samples, successfully synthesizing silver nanoparticles (Ag-NPs) that showed high susceptibility to Candida isolates. The study also revealed the resistance of Candida species to fluconazole and amphotericin B, while showing the potential of green-synthesized Ag-NPs as an anti-candida agent.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Panida Thanyasrisung, Wuttika Satitviboon, Sukanya Howattanapanich, Oranart Matangkasombut
Summary: This study aimed to investigate antifungal resistance in oral Candida isolates and the efficacy of chi-tosan, a natural polymer, against drug-resistant Candida. The results showed that chitosan was effective against most antifungal drug-resistant isolates, indicating that it could serve as a complementary antifungal agent against drug-resistant strains.
ARCHIVES OF ORAL BIOLOGY
(2023)
Article
Immunology
Walton Chan, Franklin Wang-Ngai Chow, Chi-Ching Tsang, Xueyan Liu, Weiming Yao, Tony Tat-Yin Chan, Gilman Kit-Hang Siu, Alex Yat-Man Ho, Kristine Shik Luk, Susanna Kar-Pui Lau, Patrick Chiu-Yat Woo
Summary: This research characterized the impact of extracellular vesicles (EVs) derived from Candida auris on amphotericin B resistance. The EVs significantly increased the survival of the strain against amphotericin B, but had no effect on other antifungal drugs. Mass spectrometry analysis revealed the presence of immunogenic and drug resistance-associated proteins in the EVs. These findings provide important insights into the treatment of multidrug-resistant Candida auris.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Chemistry, Medicinal
Aviran Amir, Michal Levin-Khalifa, Tamar Dvash
Summary: Fungal infections are becoming more of a problem for immunocompromised individuals, and there is evidence of new medicine-resistant strains. Researchers have discovered an ethanol amide derivative of Nystatin that shows promise as a potential drug with higher solubility and lower toxicity.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Medicine, General & Internal
Lihua Yuan, Feng Chen, Yao Sun, Yong Zhang, Xing Ji, Bo Jin
Summary: This case report highlights the successful treatment of Candida meningitis in an infant with a combination of intrathecal and intravenous amphotericin B therapy. It emphasizes the importance of considering this alternative approach for managing such a rare but severe disease.
Article
Environmental Sciences
Gabriel Davi Marena, Matheus Aparecido Dos Santos Ramos, Laura Caminitti Lima, Marlus Chorilli, Tais Maria Bauab
Summary: Galleria mellonella is a valuable model for assessing the potential of substances and studying infections caused by resistant microorganisms. Incorporating AmB into a nanoemulsion can decrease its side effects. This study developed AmB-loaded NE and evaluated its antifungal action in G. mellonella. NEA showed good antifungal properties and low toxicity in the alternative in vivo assay.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Lajos Forgacs, Andrew M. Borman, Renato Kovacs, David Balazsi, Zoltan Toth, Bence Balazs, Chiu Chun-Ju, Gabor Kardos, Ilona Kovacs, Laszlo Majoros
Summary: The efficacy of AMB against Candida auris is weak, leading to therapeutic failures. It is necessary to maximize killing at the start of treatment to avoid complications in the heart and central nervous system.
Article
Medicine, Research & Experimental
Muhammad Alif Sya'ban Mahfud, Nor Atikah Syahirah, Muhammad Akram, Ulfah Mahfufah, Mesakh Diki Saputra, Diany Elim, Maqhfirah Nurul Fitri Andi, Nurul Aisha Fitri Sultan, Achmad Himawan, Juan Dominguez-Robles, Boonnada Pamornpathomkul, Maria Mir, Andi Dian Permana
Summary: This study developed a dissolving microneedle containing solid dispersion amphotericin B (DMN-SD-AMB) for the treatment of fungal keratitis. Compared to traditional eye drops and ointments, this new formulation showed higher drug release and permeation capabilities, as well as excellent antifungal activity and safety.
MOLECULAR PHARMACEUTICS
(2023)
Article
Infectious Diseases
Jeffrey M. Rybak, Katherine S. Barker, Jose F. Munoz, Josie E. Parker, Suhail Ahmad, Eiman Mokaddas, Aneesa Abdullah, Rehab S. Elhagracy, Steve L. Kelly, Christina A. Cuomo, P. David Rogers
Summary: Candida auris has emerged as a healthcare-associated and multidrug-resistant fungal pathogen. This study identified a novel mutation in the ERG6 gene of C. auris that is associated with high-level resistance to amphotericin B. The mutation resulted in an abrogation of ergosterol biosynthesis and accumulation of cholesta-type sterols, providing insight into the mechanism of antifungal resistance in this emerging public health threat.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Engineering, Environmental
Biljana D. Skrbic, Igor Antic, Jelena Zivancev, Csaba Vagvolgyi
Summary: This study provides a comprehensive characterization of 16 polycyclic aromatic hydrocarbons (PAHs) in arable soils in northern and central Serbia as well as the cross-border region with Hungary. High molecular weight (HMW) PAHs, primarily from vehicle emissions and biomass combustion, were dominant compounds. Risk assessment indicated that farmers' exposure to PAHs through ingestion was within acceptable thresholds, with oral ingestion as the main exposure route. The study highlights the importance of comprehensive monitoring of persistent pollutants.
ENVIRONMENTAL GEOCHEMISTRY AND HEALTH
(2021)
Article
Entomology
Liliana Toth, Peter Poor, Attila ordog, Gyorgyi Varadi, Attila Farkas, Csaba Papp, Gabor Bende, Gabor K. Toth, Gabor Rakhely, Florentine Marx, Laszlo Galgoczy
Summary: Plant pathogenic fungi cause significant losses in crop production worldwide. Developing new strategies for plant and crop protection is crucial due to the resistance of these fungi to approved chemical fungicides. A study investigated the potential of using extracellular antifungal proteins (AFPs) and their peptide derivatives (PDs) as combinatorial biofungicides for plant and crop protection. The study found that the combination of AFPs and PDs can effectively inhibit the growth of various fungi and mitigate the symptoms of fungal infection on tomato plants.
Article
Agronomy
Rita Buechner, Monika Voros, Henrietta Allaga, Andras Varga, Attila Bartal, Andras Szekeres, Sarolta Varga, Judit Bajzat, Nora Bakos-Barczi, Andras Misz, Csaba Csutoras, Lorant Hatvani, Csaba Vagvolgyi, Laszlo Kredics
Summary: This study isolated and examined bacterial strains from mushroom casing material for their potential use as biocontrol agents against mold pathogens of white button mushroom. The selected Bacillus velezensis strain showed effective control against the pathogen, and even increased the crop yield of A. bisporus. These findings provide a potential method for the biological and integrated treatment in Agaricus cultivation.
Article
Microbiology
Thu Huynh, Monika Voros, Orsolya Kedves, Adiyadolgor Turbat, Gyoergy Sipos, Balazs Leitgeb, Laszlo Kredics, Csaba Vagvolgyi, Andras Szekeres
Summary: This study developed an easy and fast-automated identification tool based on cellular fatty acid profiles to distinguish Bacillus velezensis and Bacillus amyloliquefaciens. The differences and similarities between the two species were identified through the analysis of fatty acid components.
Editorial Material
Biochemistry & Molecular Biology
Gyorgyi Varadi, Laszlo Galgoczy, Gabor K. Toth
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Jeanett Holzknecht, Sandrine Dubrac, Sarah Hedtrich, Laszlo Galgoczy, Florentine Marx
Summary: Candida albicans is a common opportunistic fungal pathogen in humans, causing superficial skin and mucosal infections as well as life-threatening systemic infections. To combat emerging drug resistance, there is an urgent need to identify new antifungal compounds with novel mechanisms of action. In this study, small cationic antifungal proteins from filamentous fungi show promise as next-generation antifungals for topical application, demonstrating efficacy against cutaneous C. albicans infection in a 3D skin model.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Monika Homa, Sandugash Ibragimova, Csilla Szebenyi, Gabor Nagy, Nora Zsindely, Laszlo Bodai, Csaba Vagvolgyi, Gabor Nagy, Tamas Papp
Summary: This study reveals significant changes in gene expression of Mucor lusitanicus under aerobic and anaerobic conditions through transcriptomic analysis. Carbohydrate metabolism and transporter genes, especially sugar transporters, are found to be affected. Moreover, transcripts related to amino acid transport and metabolism, lipid transport and metabolism, and energy production and conversion are downregulated under anaerobic conditions.
Article
Microbiology
Anant Kakar, Luis Enrique Sastre-Velasquez, Michael Hess, Laszlo Galgoczy, Csaba Papp, Jeanett Holzknecht, Alessandra Romanelli, Gyorgyi Varadi, Nermina Malanovic, Florentine Marx
Summary: This study reveals the mechanism of action of TB_KKG6K on the membrane of C. albicans. The peptide compromises membrane function and enters the cells, resulting in rapid fungal cell death. This provides strong support for TB_KKG6K as a future anti-Candida therapeutic.
Article
Food Science & Technology
Alexandra Kotogan, Zsofia Terezia Furka, Tamas Kovacs, Bettina Volford, Dora Anna Papp, Monika Varga, Thu Huynh, Andras Szekeres, Tamas Papp, Csaba Vagvolgyi, Keshab Chandra Mondal, Erika Beata Kerekes, Miklos Tako
Summary: This study investigated the hydrolysis of various vegetable oils using different commercial lipases. The results showed that the lipases from R. miehei, A. niger, and R. oryzae exhibited the highest activity against olive, rapeseed, peanut, and linseed oils. The hydrolyzed oils had increased concentrations of fatty acids and improved antioxidant capacity. Furthermore, the lipase-treated oils showed inhibitory effects on the growth of food-contaminating bacteria.
Article
Food Science & Technology
Gabor Endre, Babett Edit Nagy, Daniel Hercegfalvi, Csenge Kasuba, Csaba Vagvolgyi, Andras Szekeres
Summary: Aflatoxins (AFs) are secondary metabolites that cause diseases in animals and humans. Regulations set concentration limits for AFs in EU food and feed products, requiring pure forms for reference standards. Improvements were made to a liquid-liquid chromatographic method to enhance purification and increase the yield of pure AFs. Through a scale-up process, approximately 2.2 g of AFs could be purified using 8.2 L of solvent in a 250 mL rotor, while 7.8 g of AFs could be prepared using 31 L of solvent in a 1000 mL rotor.
Article
Food Science & Technology
David Rakk, Jozsef Kukolya, Biljana D. Skrbic, Csaba Vagvolgyi, Monika Varga, Andras Szekeres
Summary: In this study, a 1-minute multi-mycotoxin analysis method was developed using high-resolution equipment and a short column. This method was able to detect 11 mycotoxins in corn and wheat matrices within one minute, utilizing the multiplexing and high-resolution capability of the QExactive Orbitrap MS.
Article
Microbiology
Viktor David Nagy, Anuar Zhumakayev, Monika Voros, Adam Borde, Adrienn Szarvas, Attila Szucs, Sandor Kocsube, Peter Jakab, Tamas Monostori, Biljana D. Skrbic, Edina Mohai, Lorant Hatvani, Csaba Vagvolgyi, Laszlo Kredics
Summary: The cultivation and consumption of sweet potato are increasing globally, but the use of chemical fertilizers and pesticides may cause pollution. Therefore, there is a need for environmentally-friendly biological solutions. This study developed a microbial inoculant from multiple microorganisms and tested its application in sweet potato cultivation, resulting in increased yield. This is the first successful application of a fungal-bacterial consortium in sweet potato cultivation.
Article
Biochemistry & Molecular Biology
Dora Balazs, Tamas Marik, Andras Szekeres, Csaba Vagvolgyi, Laszlo Kredics, Chetna Tyagi
Summary: This study investigated the production of peptaibols by various Trichoderma strains and their correlation with structure-activity relationships. The results showed that T. longibrachiatum f. bissettii SZMC 12546 exerted the lowest minimum inhibitory concentrations (MIC) against Gram-positive bacteria and also inhibited the plant pathogenic Gram-negative Rhizobium radiobacter. Molecular dynamics simulations revealed that the folding dynamics of Group A peptaibols were more restricted and had well-folded helical conformations, which may contribute to their bioactivity against bacteria.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Microbiology
Bettina Szerencses, Monika Voeroes, Kristof Bagi, Marton B. Haznagy, Attila Hunyadi, Csaba Vagvoelgyi, Ilona Pfeiffer, Mate Vagvoelgyi
Summary: Cryptococcosis, a life-threatening fungal infection that often occurs in AIDS patients, is difficult to treat due to limited effective drugs and increasing resistance. This study investigated the anti-cryptococcal activity of a natural ecdysteroid and its three derivatives obtained through structural modification. The results showed that two derivatives exhibited potent fungicidal effects, and combination with other ecdysteroids and verapamil resulted in an additive interaction.
MICROBIOLOGY RESEARCH
(2022)
