Article
Biology
Mami Tsume-Kajioka, Chiharu Kimura-Yoshida, Kyoko Mochida, Yoko Ueda, Isao Matsuo
Summary: BET proteins are essential for the specification and maintenance of the epiblast lineage during mouse preimplantation development, with BRD4 playing a central role and BRD2 acting complementarily.
Article
Biochemistry & Molecular Biology
Diane Pelzer, Ludmilla de Plater, Peta Bradbury, Adrien Eichmuller, Anne Bourdais, Guillaume Halet, Jean-Leon Maitre
Summary: Cell fragmentation is a common phenomenon in human preimplantation embryos and is associated with poor prognosis during assisted reproductive technology procedures. This study uses light sheet microscopy imaging to reveal that inefficient chromosome separation due to spindle defects, caused by dysfunctional molecular motors Myo1c or dynein, leads to cell fragmentation during mitosis. The extended exposure of the cell cortex to chromosomes triggers actomyosin contractility and pinches off cell fragments, similar to the process observed during meiosis. This study uncovers the mechanisms underlying cell fragmentation in preimplantation embryos and provides insight into the regulation of mitosis during the maternal-zygotic transition.
Article
Genetics & Heredity
Zhiyuan Chen, Mohamed Nadhir Djekidel, Yi Zhang
Summary: In mouse preimplantation embryos, H2AK119ub1 and H3K27me3 have distinct genome-wide dynamics. Loss of H2AK119ub1 results in premature activation of developmental genes, while loss of H3K27me3 does not.
Article
Cell Biology
Raquel Perez-Palacios, Maria Climent, Javier Santiago-Arcos, Sofia Macias-Redondo, Martin Klar, Pedro Muniesa, Jon Schoorlemmer
Summary: YY2, a mammalian-specific transcription factor, is expressed during mouse preimplantation development but its presence seems to be dispensable until the blastocyst stage in vitro. Depletion of Yy2 in mESCs results in increased levels of Zscan4 expression but does not affect the expression of TEs or other pluripotency markers.
Article
Developmental Biology
Luchun Zhang, Meng Yuan, Xingwei Huang, Qianzi Cao, Shaogang Huang, Ruizhen Sun, Lei Lei
Summary: Inhibiting METTL5, an enzyme that methylates 18S rRNA m(6)A, during nuclear transfer can improve the developmental rate and cell number of embryos. Inhibition of METTL5 also decreases repressive histone modification levels and increases activating histone modification and mRNA levels of specific genes.
Article
Environmental Sciences
Jiaqiao Zhu, Zhutao Huang, Fan Yang, Min Zhu, Jiangqin Cao, Jiali Chen, Yan Lin, Shuai Guo, Junwei Li, Zongping Liu
Summary: This study demonstrated that maternal cadmium exposure impairs preimplantation embryo development by disturbing epigenetic modification and inducing DNA damage. The exposure to cadmium led to embryo death, fragmentation, and developmental blockade, while affecting histone acetylation and disrupting DNA methylation of H19. Additionally, cadmium increased reactive oxygen species levels and DNA damage, while partly inhibiting gene expression related to DNA repair. Mitochondrial distribution and activity were also increased, contributing to intracellular homeostasis for embryo survival.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Yingbing Zhang, Ying Yang, Peipei Qiao, Xiyue Wang, Ruiluan Yu, Hongzheng Sun, Xupeng Xing, Yong Zhang, Jianmin Su
Summary: This study demonstrates the significant roles of CHAF1b in preimplantation embryos, possibly through regulating epigenetic modifications and lineage specification.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Hiroki Akizawa, Shun Saito, Nanami Kohri, Eri Furukawa, Yoshihiro Hayashi, Hanako Bai, Masashi Nagano, Yojiro Yanagawa, Hayato Tsukahara, Masashi Takahashi, Shinjiro Kagawa, Ryouka Kawahara-Miki, Hisato Kobayashi, Tomohiro Kono, Manabu Kawahara
Summary: This study dissected the molecular details of blastocyst stage in cattle using an optimized in vitro culture method, revealing dynamic expressions of key markers in TE cells over time. RNA-seq and qPCR analyses showed that TE cells highly expressed Wnt, FGF, and VEGF signaling pathways-related genes during their functional maturation.
Article
Multidisciplinary Sciences
Yan Bi, Zhifen Tu, Jianfeng Zhou, Xuehao Zhu, Hong Wang, Shaorong Gao, Yixuan Wang
Summary: This study investigates the cell fate dynamics during the transition from primed state to naive pluripotency using a dual fluorescent reporter system. The integration of transcription profiles and chromatin accessibility landscape reveals the appearance of primitive endoderm and trophectoderm signatures in the transitioning subpopulations. The study provides insights into early embryogenesis and offers hints for embryo modeling-related studies.
NATURE COMMUNICATIONS
(2022)
Article
Obstetrics & Gynecology
Marius Regin, Wafaa Essahib, Andrej Demtschenko, Delphine Dewandre, Laurent David, Claudia Gerri, Kathy K. Niakan, Greta Verheyen, Herman Tournaye, Johan Sterckx, Karen Sermon, Hilde van de Velde
Summary: The study investigated the processes and transcription factors involved in the first and second lineage segregation events during human preimplantation development. The findings suggest that differentiation into trophectoderm (TE) cells can be initiated independently of polarity, and TEAD1 and YAP1 co-localize in both TE and primitive endoderm (PrE) cells, indicating their role in these lineage segregation events. The research provides insights into early embryogenesis and could contribute to improving IVF practices.
HUMAN REPRODUCTION
(2023)
Article
Reproductive Biology
Alexandra Spirkova, Veronika Kovarikova, Zuzana Sefcikova, Jozef Pisko, Martina Ksinanova, Juraj Koppel, Dusan Fabian, Stefan Cikos
Summary: The study reveals that glutamic acid can affect preimplantation embryo development through cell membrane receptors. High extracellular concentrations of glutamic acid can inhibit blastocyst development, which is of practical significance.
BIOLOGY OF REPRODUCTION
(2022)
Article
Developmental Biology
Biao Ding, Di Gao, Xuegu Wang, Lei Liu, Junpei Sun, Meng Liang, Fengrui Wu, Yong Liu, Yunhai Zhang, Xiang Li, Wenyong Li
Summary: DDB1 is found to be a maternal-effect gene in porcine preimplantation embryos, and its deficiency leads to significant changes in gene expression and development, indicating its crucial role in lineage differentiation and pluripotency maintenance.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2022)
Article
Biochemistry & Molecular Biology
Yunhao Wang, Yanjiao Li, Trine Skuland, Chengjie Zhou, Aifu Li, Adnan Hashim, Ingunn Jermstad, Shaista Khan, Knut Tomas Dalen, Gareth D. D. Greggains, Arne Klungland, John Arne Dahl, Kin Fai Au
Summary: The authors used low-input methyl RNA immunoprecipitation and sequencing to map the N-6-methyladenosine (m(6)A) landscape during mouse oocyte and early embryo development. They found that retrotransposon-derived RNAs and genes involved in the maternal-to-zygotic transition are often marked by m(6)A. By applying this low-input method, they were able to define the m(6)A landscape during the maternal-to-zygotic transition, including the stage-specific expression of transcription factors that are essential for cell fate determination.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Cheng Zhao, Savana Biondic, Katherine Vandal, Asa K. Bjoerklund, Michael Hagemann-Jensen, Theresa Maria Sommer, Jesica Canizo, Stephen Clark, Pascal Raymond, Daniel R. R. Zenklusen, Nicolas Rivron, Wolf Reik, Sophie Petropoulos
Summary: The preconceptual, intrauterine, and early life environments can significantly impact the developmental trajectories and health outcomes of offspring. Assisted reproductive technologies using additives may alter blastocyst formation and affect the molecular profile and physiology of the embryo.
Article
Biochemistry & Molecular Biology
Masatoshi Ooga, Yasuyuki Kikuchi, Daiyu Ito, Kousuke Kazama, Rei Inoue, Mizuki Sakamoto, Sayaka Wakayama, Teruhiko Wakayama
Summary: Round spermatid injection (ROSI) is a last resort for men with nonobstructive azoospermia. However, ROSI has lower success rates compared to intracytoplasmic sperm injection (ICSI). This study explored histone modifications and found abnormalities in H3K36me3 in ROSI-zygotes. Treatment with TSA showed effectiveness in correcting epigenetic abnormalities.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Blanca Pijuan-Sala, Jonathan A. Griffiths, Carolina Guibentif, Tom W. Hiscock, Wajid Jawaid, Fernando J. Calero-Nieto, Carla Mulas, Ximena Ibarra-Soria, Richard C. V. Tyser, Debbie Lee Lian Ho, Wolf Reik, Shankar Srinivas, Benjamin D. Simons, Jennifer Nichols, John C. Marioni, Berthold Gottgens
Article
Physics, Multidisciplinary
Nontawit Cheewaruangroj, Karolis Leonavicius, Shankar Srinivas, John S. Biggins
PHYSICAL REVIEW LETTERS
(2019)
Article
Multidisciplinary Sciences
Ludovico Buti, Carlos Ruiz-Puig, Dennis Sangberg, Thomas M. Leissing, R. Camille Brewer, Richard P. Owen, Bruno Sgromo, Christophe Royer, Daniel Ebner, Xin Lu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Anatomy & Morphology
Cecilia G. Magalhaes, Maraysa de Oliveira-Melo, Mario C. Cruz, Shankar Srinivas, C. Y. Irene Yan
Summary: The study found that protrusions from the eye region in mouse embryos are longer than those in chick embryos. Additionally, protrusions from regions where there are no significant changes in tissue shape are longer and more stable than those surrounding the invaginating lens placode. There was no clear directionality to the protrusions observed in any region.
DEVELOPMENTAL DYNAMICS
(2021)
Article
Developmental Biology
Christophe Royer, Karolis Leonavicius, Annemarie Kip, Deborah Fortin, Kirtirupa Nandi, Anna Vincent, Celine Jones, Tim Child, Kevin Coward, Chris Graham, Shankar Srinivas
Article
Biochemistry & Molecular Biology
Navrita Mathiah, Evangeline Despin-Guitard, Matthew Stower, Wallis Nahaboo, Elif Sema Eski, Sumeet Pal Singh, Shankar Srinivas, Isabelle Migeotte
Article
Biology
Karolis Leonavicius, Christophe Royer, Antonio M. A. Miranda, Richard C. Tyser, Annemarie Kip, Shankar Srinivas
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2020)
Review
Developmental Biology
Matthew J. Stower, Shankar Srinivas
Summary: Live imaging plays a crucial role in studying early mouse embryogenesis by enabling researchers to visualize and intervene in dynamic events during development. Advances in embryo culture, microscopy hardware, and computational analysis have contributed to the development of a versatile toolkit for live imaging studies.
Article
Cell Biology
Shifaan Thowfeequ, Matthew J. Stower, Shankar Srinivas
Summary: In this review, the authors focus on the dynamic processes that emergent epithelial cells undergo during the first seven days of mouse development and speculate on the future understanding of the mechanistic bases for these processes through integrative approaches.
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2022)
Article
Multidisciplinary Sciences
Richard C. Tyser, Elmir Mahammadov, Shota Nakanoh, Ludovic Vallier, Antonio Scialdone, Shankar Srinivas
Summary: The single-cell transcriptional profile of a human embryo between 16 and 19 days after fertilization shows similarities and differences in gastrulation compared to mouse and non-human primate models. This study provides new insights into human development and offers valuable information for directed differentiation of human cells in vitro.
Article
Multidisciplinary Sciences
Christophe Royer, Elizabeth Sandham, Elizabeth Slee, Falk Schneider, Christoffer B. Lagerholm, Jonathan Godwin, Nisha Veits, Holly Hathrell, Felix Zhou, Karolis Leonavicius, Jemma Garratt, Tanaya Narendra, Anna Vincent, Celine Jones, Tim Child, Kevin Coward, Chris Graham, Marco Fritzsche, Xin Lu, Shankar Srinivas
Summary: The study reveals the important role of ASPP2 in maintaining tissue integrity and cytoskeleton organization during development, particularly in regions of high apical tension. This finding has significant implications for understanding morphogenetic events in pseudostratified epithelia and tumor suppression mechanisms.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
King Hang Tommy Mau, Donja Karimlou, David Barneda, Vincent Brochard, Christophe Royer, Bryony Leeke, Roshni A. de Souza, Melanie Pailles, Michelle Percharde, Shankar Srinivas, Alice Jouneau, Mark Christian, Veronique Azuara
Summary: The study reveals the significance of lipid droplets in the development process, particularly in the morphogenesis of blastocysts and pluripotent stem cells.
NATURE COMMUNICATIONS
(2022)
Review
Biology
Shifaan Thowfeequ, Shankar Srinivas
Summary: This article discusses the differentiation between embryonic and extraembryonic tissues in the early stages of embryonic development in eutherian mammals, highlighting the challenges and limitations of drawing discrete boundaries between the two. It also points out that basing such identity on fate is the most universal and conceptually consistent way to do so.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Editorial Material
Biology
Guojun Sheng, Thorsten E. Boroviak, Urs Schmidt-Ott, Shankar Srinivas
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Review
Cell Biology
Alyssa Kearly, Andrew D. L. Nelson, Aleksandra Skirycz, Monika Chodasiewicz
Summary: Stress Granules (SGs) and Processing-bodies (P-bodies) are important biomolecular condensates that play crucial roles in maintaining mRNA balance and regulating stress responses. They are composed of proteins and RNAs involved in translation, protein folding, and energy metabolism.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
P. Lemonnier, T. Lawson
Summary: Stomatal conductance plays a crucial role in determining CO2 uptake and water loss in plants, affecting overall water status and productivity. However, the signals coordinating mesophyll demands for CO2, the role of chloroplasts in stomatal function, and other GC metabolic processes in stomatal function remain poorly understood.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Matteo Gionfriddo, Timothy Rhodes, Spencer M. Whitney
Summary: Rubisco is a key enzyme that facilitates the entry of CO2 into the biosphere, but its catalytic properties are slow and error-prone. More effective Rubisco variants have been discovered in certain algae, offering the potential to significantly improve crop productivity. However, incompatibilities in protein folding have hindered the transplantation of these variants into plants. Directed evolution is now being explored to enhance Rubisco catalysis.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Vittoria Clapero, Stephanie Arrivault, Mark Stitt
Summary: The Calvin-Benson cycle has undergone massive selection and co-evolution with carbon-concentrating mechanisms due to changing environmental factors. Metabolite profiling reveals species-specific variations in the operation of the cycle, indicating the influence of different modes of photosynthesis. Connectivity analysis identifies constraints and driving factors for cross-species diversity in the cycle.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Sukhbir Kaur, David D. Roberts
Summary: Thrombospondin-1 modulates cell behavior by interacting with components of the extracellular matrix and cell surface receptors. Its release and expression are influenced by injuries and various diseases, while its sustained presence in the extracellular space is regulated by receptor-mediated clearance. Thrombospondin-1 plays important roles in immune responses.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Richard P. Tucker, Josephine C. Adams
Summary: Thrombospondins (TSPs) play diverse roles in animals and have been found to belong to a superfamily that includes different subgroups such as mega-TSPs, sushi-TSPs, and poriferan-TSPs. Invertebrates encode a greater diversity of TSP superfamily members than vertebrates.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
James Petrik, Sylvia Lauks, Bianca Garlisi, Jack Lawler
Summary: Many cancers start with a small nest of transformed cells that can remain dormant. Thrombospondin-1 (TSP-1) initially promotes dormancy by suppressing angiogenesis, but over time, factors promoting angiogenesis become dominant and recruit various cells to form a complex tumor microenvironment. TSPs play a role in the proliferation, migration, and invasion of cells in the tumor microenvironment, as well as influencing the immune characteristics and phenotype of tumor-associated macrophages.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Hana Fakim, Christine Vande Velde
Summary: There has been increasing attention to the role of phase-separated biomolecular condensates, specifically stress granules, in neurodegenerative diseases like ALS. ALS-associated mutations in genes involved in stress granule assembly have been found, and stress granule proteins have been detected in pathological inclusions in ALS patient neurons. However, protein components of stress granules are also present in other physiological biomolecular condensates, which have not been adequately discussed in relation to ALS. This review explores the functions of TDP-43 and FUS in physiological condensates occurring in the nucleus and neurites beyond stress granules, and discusses the impact of ALS-linked mutations on their ability to phase separate and perform their functions in stress-independent biomolecular condensates.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Alexander Lin, Yogambha Ramaswamy, Ashish Misra
Summary: Smooth muscle cells, endothelial cells, and macrophages in blood vessels display remarkable heterogeneity, and their developmental origins may influence their plasticity. Unbiased single cell whole transcriptome analysis techniques are revolutionizing the understanding of cellular diversity and plasticity, providing insights for therapeutic research.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Elton P. Hudson
Summary: The Calvin Benson cycle plays a crucial role in the ecological and biotechnological aspects of bacteria. Recent studies have shed light on the regulation of this cycle in bacteria, with post-transcriptional and post-translational regulation being important in phototrophic bacteria, and transcriptional regulation being prominent in chemolithoautotrophic bacteria. Understanding the regulation of the Calvin Benson cycle has implications for enhancing CO2 fixation and improving the synthesis of desired products. Non-canonical cycles may offer potential benefits for industrial applications.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Xin-Guang Zhu, Haim Treves, Honglong Zhao
Summary: This paper discusses the major regulatory mechanisms over the Calvin Benson Cycle (CBC) that maintain homeostasis of metabolite levels. These mechanisms include redox regulation of enzymes, metabolite regulations (especially allosteric regulations), and balanced activities of enzymes. These regulatory mechanisms are crucial for maintaining high flux and photosynthetic efficiency in CBC.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Hunter C. Herriage, Yi-Ting Huang, Brian R. Calvi
Summary: Apoptosis prevents the inappropriate acquisition of extra copies of the genome, known as polyploidy, but the polyploid state can suppress apoptosis. The mechanisms linking apoptosis and polyploid cell cycles are still largely unknown, and studying the regulation of apoptosis in development and cancer could lead to more effective therapies.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Daniel Campbell, Steven Zuryn
Summary: Mitochondrial dynamics play a crucial role in regulating cellular and organismal homeostasis, impacting various aspects of an organism's healthspan. By studying the nematode Caenorhabditis elegans, a comprehensive understanding of the impact of mitochondrial dynamics on homeostasis over a lifetime can be obtained.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Diba Borgmann, Henning Fenselau
Summary: Maintaining blood glucose at an appropriate physiological level requires coordination of multiple organs and tissues, with the vagus nerve playing a key role in central control. Recent studies have revealed the cellular identity, neuroanatomical organization, and functional contributions of vagal neurons in the regulation of systemic glucose metabolism. These findings provide new insights into the precise roles of vagal neurons in coordinating glucose levels and offer potential avenues for treating glucose metabolism disorders.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Tatiana C. Coverdell, Stephen B. G. Abbott, John N. Campbell
Summary: In this article, we review how genetic technology and single-cell genomics are revealing the organizational principles of the efferent vagus in unprecedented detail.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
