Article
Biochemistry & Molecular Biology
Wu Jiang, Fan He, Guoming Ding, Junsong Wu
Summary: This study investigated the effects of Top1 inhibition in a spinal cord injury (SCI) model. The results showed that administration of the Top1 inhibitor CPT improved locomotor functional recovery, reduced neuronal loss, and decreased neutrophil infiltration in mice with SCI. Additionally, CPT inhibited pyroptosis and suppressed neuroinflammation both in vivo and in vitro.
Article
Medicine, Research & Experimental
Bo Xu, Jiaqi Fang, Jianguang Wang, Xuehan Jin, Shengfu Liu, Kaihang Song, Ping Wang, Junjian Liu, Shuhao Liu
Summary: This study investigated the protective effect of edaravone on blood spinal cord barrier (BSCB) disruption after spinal cord injury (SCI). The results showed that edaravone treatment promoted functional recovery, improved vascular damage, and up-regulated BSCB-associated proteins in rats with SCI. In vitro experiments also demonstrated that edaravone improved cell viability, restored intercellular junctions, and promoted angiogenic activities. The underlying mechanism may involve the improvement of autophagy and the phosphorylation of RIP1/RIP3/MLKL. Overall, edaravone can be a potential treatment option for SCI.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Neurosciences
Susana R. Cerqueira, Sofia Benavides, Ha Eun Lee, Nagi G. Ayad, Jae K. Lee
Summary: Inhibiting BET proteins after spinal cord injury (SCI) can reduce lesion size and provide neuroprotection, but the improvement observed is not related to macrophages. Further research on the role of BET proteins after SCI is necessary to identify new therapeutic targets.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Cell & Tissue Engineering
Nishanth Lakshman, Clara Bourget, Ricky Siu, Vladimir V. Bamm, Wenjun Xu, George Harauz, Cindi M. Morshead
Summary: The study demonstrates that myelin basic protein (MBP) can regulate neural precursor cell (NPC) behavior and that the inhibitory effects of MBP on NPCs are regionally distinct. The heat-labile protein released by spinal cord niche cells plays a key role in mediating the inhibitory actions of MBP on NPCs. These findings highlight the importance of MBP and regionally distinct microenvironments in regulating NPC behavior, with implications for stem cell-based regenerative strategies.
Article
Cell Biology
Natalia Kulesskaya, Ekaterina Mugantseva, Rimante Minkeviciene, Natalia Acosta, Ari Rouhiainen, Juha Kuja-Panula, Mikhail Kislin, Sami Piirainen, Mikhail Paveliev, Heikki Rauvala
Summary: Protamine and its fragment LMWP can overcome the inhibition of CSPGs on neurite outgrowth and show good biocompatibility and low toxicity. LMWP is a promising drug lead for the treatment of CNS injuries.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Jin Huang, Pan Chen, Ke Liu, Jiao Liu, Borong Zhou, Runliu Wu, Qiu Peng, Ze-Xian Liu, Changfeng Li, Guido Kroemer, Michael Lotze, Herbert Zeh, Rui Kang, Daolin Tang
Summary: Inhibition of CDK1/2/5 kinase activity can overcome IFNG-mediated immune evasion in pancreatic tumors, turning a 'cold' tumor microenvironment into a 'hot' one and significantly improving overall survival rates in mouse pancreatic tumor models. Abnormal expression of CDK1/2/5 and IDO1 is associated with poor patient survival in various cancer types, including PDAC.
Article
Engineering, Biomedical
Xu Gao, Zhifeng You, Yue Li, Xinyi Kang, Wen Yang, Huiru Wang, Ting Zhang, Xinhao Zhao, Yifu Sun, He Shen, Jianwu Dai
Summary: In this study, a functional hybrid gelatin and hyaluronic acid hydrogel (F-G/H) modified with cationic polymers and anti-inflammatory cytokines was developed to regulate the immune microenvironment after aSCT implantation. The hydrogel exhibited DAMP scavenging capacity, sustained release of anti-inflammatory cytokines, and reduced lymphocyte accumulation, thereby enhancing the survival and function of aSCTs. When combined with systemic immunosuppressive drug treatment, a significant improvement in locomotor functions of SCI rats was observed after aSCTs and F-G/H transplantation.
ACTA BIOMATERIALIA
(2023)
Article
Neurosciences
R. Moreno-Luna, P. F. Esteban, B. Paniagua-Torija, A. Arevalo-Martin, D. Garcia-Ovejero, E. Molina-Holgado
Summary: Regional differences exist in some components of the endocannabinoid system between oligodendrocyte precursor cells (OPCs) and mature oligodendrocytes (OLs) derived from the spinal cord and cerebral cortex, which need to be taken into consideration in therapeutic strategies.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Cell Biology
Angela D. Morris, Sarah Kucenas
Summary: Injecting lysolecithin into zebrafish larvae allows for visualization of key features of lysolecithin-induced demyelination, similar to mammalian models, providing a new approach to study the temporal dynamics of such damage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Amy R. McDowell, Natalia Petrova, Daniele Carassiti, Marc E. Miquel, David L. Thomas, Gareth J. Barker, Klaus Schmierer, Tobias C. Wood
Summary: This study investigated the use of magnetic resonance imaging to visualize myelin in postmortem multiple sclerosis spinal cord. The high-resolution MR parameter maps showed excellent correspondence with histological markers.
MAGNETIC RESONANCE IN MEDICINE
(2022)
Article
Neurosciences
Chengjie Wu, Yalan Pan, Lining Wang, Mengmin Liu, Pengcheng Tu, Sixian Chen, Lei Shi, Danqing Yan, Yong Ma, Yang Guo
Summary: This study aimed to find an effective strategy for promoting microvascular endothelial cells (MECs) to phagocytize myelin debris and reduce secretion of inflammatory factors following spinal cord injury (SCI). A coculture model of myelin debris and vascular-like structures was established, and the efficiency of MECs phagocytizing myelin debris under different conditions was examined. The HDAC6 inhibitor Tubastatin-A was used to interfere with the coculture model, and its anti-inflammatory effects were observed.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Multidisciplinary Sciences
Se Jin Oh, Ji Yeon Lim, Min Kyu Son, Jun Hyeok Ahn, Kwon-Ho Song, Hyo-Jung Lee, Suyeon Kim, Eun Ho Cho, Joon-Yong Chung, Hanbyoul Cho, Hyosun Kim, Jae-Hoon Kim, Jooyoung Park, Jungmin Choi, Sun Wook Hwang, Tae Woo Kim
Summary: The emergence of cisplatin resistance and side-effects are important factors in cancer treatment failure. The authors identify autophagy-mediated EGFR hyperactivation by TRPV1 promotion as a mechanism of cisplatin resistance and demonstrate the efficacy of a TRPV1 inhibitor to sensitize lung cancer to cisplatin. Future studies should provide evidence regarding the mechanisms of potential targets that can overcome the resistance as well as alleviate pain.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Bowen Wan, Cong Li, Ming Wang, Fanqi Kong, Qirui Ding, Chenliang Zhang, Hao Liu, Dingfei Qian, Wenlin Deng, Jian Chen, Pengyu Tang, Qian Wang, Shujie Zhao, Zheng Zhou, Tao Xu, Yifan Huang, Jun Gu, Jin Fan, Guoyong Yin
Summary: The study demonstrates the crucial role of GIT1 in promoting MECs clearance of myelin debris and stimulating angiogenesis, making it a promising target for accelerating SCI recovery.
Review
Biochemistry & Molecular Biology
Filippo Pinelli, Fabio Pizzetti, Valeria Veneruso, Emilia Petillo, Michael Raghunath, Giuseppe Perale, Pietro Veglianese, Filippo Rossi
Summary: Spinal cord injury is a damaging process that occurs during an acute traumatic event, and requires a multi-target approach for treatment. Recent advances in biomaterials and drug/biomolecule combinations have shown promise in the treatment of spinal cord injuries.
Article
Chemistry, Multidisciplinary
Kaixuan Zeng, Weihao Li, Yue Wang, Zifei Zhang, Linjie Zhang, Weili Zhang, Yue Xing, Chi Zhou
Summary: This study identifies CDK1 as a critical contributor to oxaliplatin resistance in colorectal cancer (CRC) by suppressing ferroptosis. CDK1 is highly expressed in oxaliplatin-resistant cells and tissues due to the loss of N6-methyladenosine modification. Inhibition of CDK1 restores sensitivity to oxaliplatin in CRC cells and xenograft models. Mechanistically, CDK1 phosphorylates and promotes ubiquitination of ACSL4, leading to its degradation and subsequent inhibition of lipid peroxidation and ferroptosis. These findings suggest that CDK1 inhibition may be a promising strategy for treating oxaliplatin-resistant CRC.