Article
Biochemistry & Molecular Biology
Bo Yu, Helena T. A. van Tol, Christine H. Y. Oei, Tom A. E. Stout, Bernard A. J. Roelen
Summary: The study demonstrates that lysophosphatidic acid (LPA) can accelerate bovine blastocyst formation, leading to up-regulation of TAZ and TEAD4 expression, as well as overexpression of YAP. However, the LPA-induced increase in CDX2 expression was only significant in day 8 embryos.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Simone Johansen, Sofie Traynor, Malene Laage Ebstrup, Mikkel Green Terp, Christina Bog Pedersen, Henrik Jorn Ditzel, Morten Frier Gjerstorff
Summary: The transcription factor ZBED1 is highly expressed in trophoblast cells and may play important roles in placental biology, including maintaining a steady pool of cytotrophoblast cells, regulating trophoblast cell survival and apoptosis, and modulating maternal immune tolerance.
Article
Cell Biology
Yinjuan Wang, Hao Ming, Leqian Yu, Jie Li, Linkai Zhu, Hai-Xi Sun, Carlos A. Pinzon-Arteaga, Jun Wu, Zongliang Jiang
Summary: A chemical cocktail enables the derivation and long-term culture of bovine trophoblast stem cells, which possess the potential to differentiate into mature trophoblast cells and exhibit similar characteristics to trophectoderm cells from early bovine embryos. These bovine TSCs provide a valuable model to study bovine placentation and early pregnancy failure.
Article
Multidisciplinary Sciences
Mai Sato, Asako Inohaya, Eriko Yasuda, Haruta Mogami, Yoshitsugu Chigusa, Kaoru Kawasaki, Yosuke Kawamura, Yusuke Ueda, Hiroshi Takai, Masaki Mandai, Eiji Kondoh
Summary: A three-dimensional placental organ bud was successfully generated from human iPSCs and transplanted into the murine uterus, showing a 22% engraftment success rate. These iPSC-derived placental organ buds could serve as a new model for studying placental function and pathology in research.
SCIENTIFIC REPORTS
(2021)
Article
Developmental Biology
Yan Shi, Bingjie Hu, Zizengchen Wang, Xiaotong Wu, Lei Luo, Shuang Li, Shaohua Wang, Kun Zhang, Huanan Wang
Summary: The effects of GATA3 and CDX2, trophectoderm-associated factors, on lineage specification during early embryonic development in cattle were studied. Using a cytosine base editor system, the effects of these factors on the expression of lineage specification marker genes were determined. Knockout of GATA3 or CDX2 did not affect the developmental potential of embryos, but GATA3 deletion downregulated NANOG expression in bovine blastocysts. Further analysis showed that GATA3 is required for NANOG in the trophectoderm of bovine blastocysts. Single blastocyst RNA-seq analysis revealed that GATA3 deletion disrupts the transcriptome in bovine blastocysts.
Article
Reproductive Biology
Mariangela Bueno Cordeiro Maldonado, Valeska de Castro Lourenco, Lucas de Oliveira Bezerra, Isabella Rio Feltrin, Adriano Felipe Mendes, Cecilia Constantino Rocha, Guilherme Pugliesi, Alan D. Ealy, Claudia Maria Bertan Membrive, Marcelo Fabio Gouveia Nogueira
Summary: Early embryonic mortality due to maternal-fetal recognition failure is a major issue in the cattle industry. Modifying the levels and ratios of prostaglandin F2a and PGE2 can improve pregnancy establishment in cattle. This study investigated the effects of conjugated linoleic acid (CLA) on PGE2 and PGF2a synthesis and gene expression related to maternal-fetal recognition in bovine trophoblast cells (CT-1). CLA supplementation decreased PGE2 and PGF2a concentrations and influenced the ratio of PGE2 to PGF2a in CT-1. CLA also had a quadratic effect on the expression of MMP9, PTGES2, and PTGER4 genes.
Article
Cell Biology
Shuai Yu, Rui Zhang, Qiaoyan Shen, Zhenshuo Zhu, Juqing Zhang, Xiaolong Wu, Wenxu Zhao, Na Li, Fan Yang, Hongjiang Wei, Jinlian Hua
Summary: By overexpressing the transcription factor ESRRB, flat, monolayered piPSCs were created with enhanced chimeric capacity into the trophectoderm. ESRRB directly regulates the expressions of trophoblast stem cell markers, and its cooperation with OCT4 facilitates the transition from pluripotent state to trophoblast-like state.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shun Saito, Shota Yamamura, Nanami Kohri, Hanako Bai, Masashi Takahashi, Manabu Kawahara
Summary: WW domain-containing transcription regulator 1 (WWTR1) is a key player in the Hippo pathway and plays a crucial role in the differentiation of trophectoderm (TE) cells during preimplantation development. Knockdown of WWTR1 resulted in reduced TE cell numbers and expression of TE-specific genes in bovine embryos, highlighting the importance of WWTR1 in TE cell development.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Developmental Biology
Viju Vijayan Pillai, Tiffany G. Kei, Shailesh Gurung, Moubani Das, Luiz G. B. Siqueira, Soon Hon Cheong, Peter J. Hansen, Vimal Selvaraj
Summary: TGF beta 1 plays a crucial role in the differentiation of trophoblast cells, and inhibition of RhoA/Rock promotes self-renewal and proliferation.
Article
Biochemistry & Molecular Biology
Shuai Yu, Qiaoyan Shen, Rui Zhang, Xiaolong Wu, Juqing Zhang, Wenxu Zhao, Xiaojie Wu, Na Li, Sha Peng, Shiqiang Zhang, Fan Yang, Jinlian Hua
Summary: This study investigates the global changes in histone H3 Lysine 4, 9, 27, 36 methylation and Lysine 27 acetylation in piPSCs and TLSCs. It shows that TLSCs have a high modification profile of H3K36me2 compared to piPSCs. The study also reveals the upregulation of KDM4C, a H3K36me3/2 demethylase, in TLSCs, which plays a crucial role in regulating TLSCs' pluripotency and proliferation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Eiji Ogawa, Yo Oguma, Yoshihiro Kushida, Shohei Wakao, Kana Okawa, Mari Dezawa
Summary: Multilineage-differentiating stress-enduring (Muse) cells are a type of non-tumorigenic pluripotent-like stem cells that can undergo triploblastic differentiation and self-renewal at the single-cell level. Human amniotic membrane mesenchymal stem cells (hAMSCs) exhibit unique characteristics compared to human bone marrow (hBM)-Muse cells, including differential gene expression and broader differentiation potential.
SCIENTIFIC REPORTS
(2022)
Article
Cell & Tissue Engineering
Ge Guo, Giuliano Giuseppe Stirparo, Stanley E. Strawbridge, Daniel Spindlow, Jian Yang, James Clarke, Anish Dattani, Ayaka Yanagida, Meng Amy Li, Sam Myers, Buse Nurten Ozel, Jennifer Nichols, Austin Smith
Summary: Classic embryological experiments have shown that early mouse embryos develop through sequential lineage bifurcations. However, human naive embryonic stem cells exhibit the ability to produce blastocyst trophectoderm, unlike their mouse counterparts. This suggests a difference in developmental specification and lineage restriction between human and mouse embryos.
Review
Cell Biology
Alexandra Calle, Miguel Angel Ramirez
Summary: Bovine mesenchymal stem cells play an important immunomodulatory role in the maternal reproductive tract, preventing embryo rejection. They interact with embryonic trophoblast through the exchange of extracellular vesicles (EVs) and soluble proteins, facilitating successful implantation. However, the effects of viral infections on this communication between maternal and embryonic MSC populations are not well understood.
Article
Multidisciplinary Sciences
Chen Dong, Shuhua Fu, Rowan M. Karvas, Brian Chew, Laura A. Fischer, Xiaoyun Xing, Jessica K. Harrison, Pooja Popli, Ramakrishna Kommagani, Ting Wang, Bo Zhang, Thorold W. Theunissen
Summary: The authors systematically identify and characterize essential and growth-restricting genes in human trophoblast cells through a genome-wide CRISPR-Cas9 knockout screen. They reveal TEAD1 as a key regulator modulating chromatin architecture and gene expression to play a crucial role in the specification, maintenance, and differentiation of the human trophoblast lineage.
NATURE COMMUNICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Dejun Xu, Xiaohan Jiang, Yukun Wang, Shuaifei Song
Summary: LRH-1 is highly expressed in bovine granulosa cells of atretic follicles. It induces apoptosis of the cells through downregulating the progestogen receptor signaling pathway, thus modulating bovine follicular atresia.
Article
Multidisciplinary Sciences
Carol L. Keefer
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Developmental Biology
M. E. Brown, S. J. Converse, J. N. Chandler, A. L. Crosier, W. Lynch, D. E. Wildt, C. L. Keefer, N. Songsasen
REPRODUCTION FERTILITY AND DEVELOPMENT
(2017)
Article
Endocrinology & Metabolism
Megan E. Brown, Sarah J. Converse, Jane N. Chandler, Charles Shafer, Janine L. Brown, Carol L. Keefer, Nucharin Songsasen
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2016)
Article
Cell Biology
Andrew T. Schiffmacher, Vivien Xie, Lisa A. Taneyhill
JOURNAL OF CELL BIOLOGY
(2016)
Article
Reproductive Biology
Lindsey M. Vansandt, Janelle L. Livesay, Melissa Joy Dickson, Lei Li, Budhan S. Pukazhenthi, Carol L. Keefer
Review
Developmental Biology
Lisa A. Taneyhill, Andrew T. Schiffmacher
Article
Developmental Biology
Paweena Thuwanut, Pierre Comizzoli, David E. Wildt, Carol L. Keefer, Nucharin Songsasen
REPRODUCTION FERTILITY AND DEVELOPMENT
(2017)
Article
Biology
Megan E. Brown, Ram P. Singh, Budhan Pukazhenthi, Carol L. Keefer, Nucharin Songsasen
Article
Developmental Biology
Andrew T. Schiffmacher, Ashrifia Adomako-Ankomah, Vivien Xie, Lisa A. Taneyhill
DEVELOPMENTAL BIOLOGY
(2018)
Article
Agriculture, Dairy & Animal Science
C. Wynne Collins, Steven L. Monfort, Mandi M. Vick, Barbara A. Wolfe, Rachael B. Weiss, Carol L. Keefer, Nucharin Songsasen
ANIMAL REPRODUCTION SCIENCE
(2014)
Article
Reproductive Biology
Halli S. Weiner, Adrienne E. Crosier, Carol L. Keefer
BIOLOGY OF REPRODUCTION
(2019)
Article
Biochemistry & Molecular Biology
Ran Zhou, Pierre Comizzoli, Carol L. Keefer
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2019)
Article
Biotechnology & Applied Microbiology
Jaewook Chung, Ganesh Sriram, Carol L. Keefer
BIOTECHNOLOGY LETTERS
(2020)
Article
Multidisciplinary Sciences
Ceren Pajanoja, Jenny Hsin, Bradley Olinger, Andrew Schiffmacher, Rita Yazejian, Shaun Abrams, Arvydas Dapkunas, Zarin Zainul, Andrew D. Doyle, Daniel Martin, Laura Kerosuo
Summary: The entire post-gastrula ectoderm maintains expression of pluripotency genes, explaining the high stem cell potential in the neural crest.
NATURE COMMUNICATIONS
(2023)
Article
Developmental Biology
Ankita Shah, Andrew T. Schiffmacher, Lisa A. Taneyhill
DEVELOPMENTAL BIOLOGY
(2017)