Article
Plant Sciences
Laudecir L. Raiol-Junior, Juan C. Cifuentes-Arenas, Nik J. Cunniffe, Robert Turgeon, Silvio A. Lopes
Summary: The study found that the movement speed of Las in plants is between 2.9-3.8 cm per day, and the colonization of plants by Las is influenced by temperature, with 25.7 degrees Celsius being the optimal condition. This explains the higher impact and incidence of huanglongbing disease during the winter months or regions of milder climates in Brazil.
Review
Microbiology
Bin Hu, Muhammad Junaid Rao, Xiuxin Deng, Sheo Shankar Pandey, Connor Hendrich, Fang Ding, Nian Wang, Qiang Xu
Summary: Citrus Huanglongbing (HLB), also known as citrus greening, is a devastating citrus disease with no commercial citrus cultivars resistant to it. The most prevalent strain associated with HLB, Candidatus Liberibacter asiaticus (CLas), has yet to be cultured in vitro. Research has focused on the complex pathosystem of Ca. Liberibacter, including the interaction of host and CLas, and the impact of CLas infection on host transcripts, proteins, and metabolism. Identifying candidate genes for CLas pathogenicity and improving HLB tolerance in citrus are key research areas for the future.
Article
Plant Sciences
Yang Hu, Youqing Meng, Liangjin Yao, Enguo Wang, Tao Tang, Yunsheng Wang, Liangying Dai, Mingping Zhao, Hong-en Zhang, Xiaoyan Fan, Luyun Luo, Wei Xiang, Zhuo Zhang
Summary: This study systematically analyzed the factors related to citrus Huanglongbing and found that the number of bacteria carried by Diaphorina citri and the changes in citrus phyllosphere microbiome are correlated with the incidence of Huanglongbing.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Chunzhen Cheng, Yun Zhong, Bin Wang, Yongyan Zhang, Huan Wu, Nonghui Jiang, Bo Wu, Yuanda Lv, Bo Jiang
Summary: This study found that the upregulated expression of the RIN4 gene in citrus infected with HLB may aid the infection of Candidatus Liberibacter asiaticus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Laudecir L. Raiol-Junior, Juan C. Cifuentes-Arenas, Everton de Carvalho, Eduardo A. Girardi, Silvio A. Lopes
Summary: Las, associated with HLB, moves laterally through phloem in plants, predominantly towards newly developing root and leaf tissues. Protecting rapidly growing shoots from feeding by D. citri is crucial to minimize transmission and spread of the pathogen.
Article
Microbiology
Marcelo L. Merli, Kaylie A. Padgett-Pagliai, Alexandra E. Cuaycal, Lucila Garcia, Maria Rosa Marano, Graciela L. Lorca, Claudio F. Gonzalez
Summary: The study revealed that a potential effector LotP of 'Candidatus Liberibacter asiaticus' may interact with chaperones like GroEL and Hsp40 to enhance Lon protease activity, leading to damage in citrus plant tissues. LotP may also interact with plant proteins, indicating its potential role in protease activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Yongqin Zheng, Jun Guo, Xiaoling Deng, Zheng Zheng
Summary: The draft genome sequence of a 'Candidatus Liberibacter asiaticus' strain isolated from an HLB-affected lime tree in Myanmar was reported, enriching the current genome database and facilitating research on HLB epidemiology in Asia and beyond.
Article
Plant Sciences
Wen-Shan Dai, Ting Peng, Min Wang, Ji-Hong Liu
Summary: This study systematically analyzed the genomic characteristics and expression patterns of the WRKY transcription factor family in two Citrus species, with a focus on their expression patterns under SA signaling and CLas infection. The results provided a foundation for further research on the function of WRKY TFs in HLB response and SA signaling in Citrus.
Article
Agronomy
Minli Bao, Zheng Zheng, Jianchi Chen, Xiaoling Deng
Summary: Huanglongbing (HLB) is a devastating disease affecting citrus production worldwide, and in China it is associated with the unculturable alpha-proteobacterium Candidatus Liberibacter asiaticus (CLas). Phages/prophages of CLas have been identified through genomic research, aiding in understanding CLas biology. This study investigated the symptom development of different citrus cultivars infected with CLas strains carrying Type 1 and Type 2 phages, providing baseline information for future research on the roles of CLas phages in HLB symptom development.
Article
Plant Sciences
Manjunath L. Keremane, Thomas G. McCollum, Mikeal L. Roose, Richard F. Lee, Chandrika Ramadugu
Summary: An improved reference gene, malate dehydrogenase (MDH), has been developed for sensitive detection of the CLas bacterium in plants, suitable for diagnostic duplex qPCR and analytical digital droplet PCR (ddPCR) assays.
Article
Plant Sciences
Fengnian Wu, Lihui Liu, Eduardo G. P. Fox, Xiaoling Deng, Meirong Xu, Zheng Zheng, Xiuhong Li, Jiawei Fu, Hui Zhu, Jianjian Huang, Tiejun Deng
Summary: This study investigated the effects of 'Ca. L. asiaticus' infection on key physiological variables in citrus plants. Results showed that most variables, except soluble sugar, were affected by increased infection titers, with the variety C. maxima showing greater resistance. The infection was linked to a decrease in chlorophyll concentration and cell membrane integrity, and an increase in free proline and starch contents.
Article
Plant Sciences
P. A. Silva, J. Huang, N. A. Wulff, Z. Zheng, R. Krugner, J. Chen
Summary: This study presents the draft whole-genome sequence of 'Ca. L. asiaticus' strain 9PA from a sweet orange tree in Sao Paulo State, Brazil, providing valuable genetic resources for research and control of HLB.
Article
Plant Sciences
Emily M. T. Padhi, Karla J. Araujo, Elizabeth Mitrovic, Marylou Polek, Kris E. Godfrey, Carolyn M. Slupsky
Summary: This study used a metabolomics approach to investigate the changes in leaf metabolites during the early stages of Huanglongbing (HLB). The results showed that infection with Candidatus Liberibacter asiaticus caused significant alterations in leaf metabolome, enabling discrimination between healthy and infected plants.
Article
Plant Sciences
Yanjing Wang, Takumasa Kondo, Yurong He, Zhuohuai Zhou, Jinming Lu
Summary: The study presents the genome sequence of CLas strain CoFLP1 collected from Colombia, contributing to the understanding of the introduction pathway of HLB in South America. This first CLas genome sequence from Colombia will enhance CLas genome resources.
Article
Multidisciplinary Sciences
Maria Quintana-Gonzalez de Chaves, Felix Moran, Silvia Barbe, Edson Bertolini, Felipe Siverio de la Rosa, Ester Marco-Noales
Summary: A new quantitative real-time PCR protocol has been developed to detect four known phytopathogenic species of the genus Liberibacter, which shows higher specificity and sensitivity compared to previous protocols. It can be used as a rapid and time-saving screening test for simultaneous detection of all plant pathogenic species of 'Ca. Liberibacter' in a one-step assay.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, General & Internal
Krist H. Antunes, Renato T. Stein, Caroline Franceschina, Emanuelle F. da Silva, Deise N. de Freitas, Josiane Silveira, Magali Mocellin, Lidiane Leitao, Jose L. Fachi, Lais P. Pral, Amanda Gonzalez, Sarah Oliveira, Leonardo Duarte, Gisele Cassao, Joao I. B. Goncalves, Tatiane M. Reis, Bruno L. Abbadi, Maiele Dornelles, Nathalia D. M. Sperotto, Mauricio Rigo, Hosana Rodrigues, Marcus Jones, Matias Epifanio, Suzana Guima, Joao C. Setubal, Taissa R. Jorge, Daniel S. Mansur, Fabiana Q. Mayer, Ana Paula M. Varela, Cristiano Bizarro, Pablo Machado, Luiz A. Basso, Fernando P. Polack, Adnan Custovic, Marco A. R. Vinolo, Ana Paula D. de Souza
Summary: Gut microbiota-derived short-chain fatty-acid (SFCA) acetate regulates the expression of RIG-I and reduces the severity and viral load of RSV infection. The study found that treatment with SCFA-acetate can decrease RSV infection and viral load of clinical isolates, and increase the expression of interferon-stimulated genes.
Article
Entomology
Victor H. Nagatani, Manuela O. Ramalho, Juliana M. C. Alves, Rodrigo F. Souza, Debora Y. Kayano, Nathalia S. Silva, Otavio G. M. Silva, Ricardo Harakava, Odair C. Bueno, Alexandre W. S. Hilsdorf, Maria S. C. Morini
Summary: This study found that the occurrence and molecular diversity of Solenopsis invicta and Solenopsis saevissima, two common fire ant species in Brazil, are influenced by the native vegetation cover near crops. Interestingly, S. invicta showed no limitation by vegetation cover and had higher haplotype diversity compared to S. saevissima, especially when the vegetation cover was above 50%.
AGRICULTURAL AND FOREST ENTOMOLOGY
(2023)
Article
Mycology
Nathalie Otoya-Martinez, Luis Garrigos Leite, Ricardo Harakava, Mustapha Touray, Selcuk Hazir, Julie Chacon-Orozco, Cesar Junior Bueno
Summary: The inhibitory effects of certain Trichoderma strains and cell-free supernatants (CFS) from Xenorhabdus and Photorhabdus bacteria against N. parvum in grapevines were investigated. Trichoderma strains and X. szentirmaii PAM 11 showed promising inhibitory effects in pruning wounds of grapevine shoots. This study highlights the potential of Trichoderma and X. szentirmaii PAM 11 as biofungicides for controlling N. parvum in grapevines.
Article
Biotechnology & Applied Microbiology
Matheus Luis Docema, Tatiana de Souza Moraes, Lisia Borges Attilio, Joao Paulo Rodrigues Marques, Liliane Cristina Liborio Stipp, Ricardo Harakava, Juliana Freitas-Astua, Jose Belasque Junior, Ricardo Antunes Azevedo, Salete Aparecida Gaziola, Francisco de Assis Alves Mourao Filho
Summary: Currently, there are no resistant sweet orange cultivars to citrus canker. Transgenic plants expressing antimicrobial peptides and resistance genes from tolerant cultivars were evaluated for resistance to Xanthomonas citri subsp. citri (Xcc). Transgenic plants showed reduced incidence and severity of citrus canker, as well as lower Xcc titers. Higher superoxide dismutase activity in transgenic plants led to increased hydrogen peroxide production. Callose deposition, an important plant defense response, was observed in transgenic plants with lower Xcc populations.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Infectious Diseases
Luis Antonio Rodriguez Carnero, Andreia Kuramoto, Lea Campos de Oliveira, Jhonatas Sirino Monteiro, Joao Carlos Setubal, Edecio Cunha-Neto, Ester Cerdeira Sabino, Ricardo Jose Giordano
Summary: This study used the gPhage platform to compare antigens and antibodies before and after treatment in 20 patients with chronic Chagas disease. The results showed a decrease in antibody levels and recognition of specific antigens after treatment. The gPhage data correlated with patient PCR-status and could predict patient response to treatment. In conclusion, the use of the gPhage platform contributes to the development of novel diagnostic tools for Chagas disease.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Biodiversity Conservation
Manuela O. Ramalho, Victor H. Nagatani, Juliana M. C. Alves, Otavio G. M. Silva, Eduardo G. P. Fox, Rodrigo F. Souza, Debora Y. Kayano, Ricardo Harakava, Alexandre W. S. Hilsdorf, Maria S. C. Morini
Summary: The investigation studied the diversity of fire ants in their native range, focusing on the Red Imported Fire Ant Solenopsis invicta. The study revealed that this species is gradually spreading to other regions of Brazil from its original habitat in South America. The survey of S. invicta populations in Sao Paulo, Brazil, using mtDNA COI haplotypes identified four haplotypes, indicating the occurrence of two separate expansion waves of S. invicta. The mountain range Serra do Mar may act as a geographical barrier affecting gene flow.
Article
Parasitology
Deborah Mirela de Melo Romano, Thiago Nunes Pereira, Isabella Barboza de Almeida, Caroline Santos Gambini Coelho, Fernanda Calvo Duarte, Ricardo Harakava, Luara Lucena Cassiano, Marcia Cristina Mendes
Summary: The study aimed to investigate the presence of Wolbachia in Amblyomma sculptum ticks. Molecular techniques and bioinformatics tools were used to analyze 187 adult ticks collected from two municipalities in Sao Paulo, Brazil. Fifteen ticks were found to be positive for Wolbachia. This study is the first to report the occurrence of Wolbachia in A. sculptum and adds to the understanding of its susceptibility to this bacterium.
VETERINARY PARASITOLOGY
(2023)
Article
Microbiology
Nitin K. Singh, Jason M. Wood, Jose Patane, Livia Maria Silva Moura, Jonathan Lombardino, Joao Carlos Setubal, Kasthuri Venkateswaran
Summary: In this study, metagenome-assembled genomes (MAGs) were generated to analyze the microbial diversity and functional properties of the International Space Station (ISS) environment. The results showed that ISS samples contained 18 bacterial species and 4 fungal genomes. The study revealed evolution of microbes in space, enrichment of genes related to cell membranes, and higher prevalence of antimicrobial resistance and virulence genes in certain bacteria. Comparative analysis indicated that Pantoea/Kalamiella strains are more susceptible to microgravity changes. These findings contribute to understanding microbial evolution under microgravity and radiation pressure.
Article
Multidisciplinary Sciences
Alex Inague, Lilian Costa Alecrim, Jhonatas Sirino Monteiro, Marcos Yukio Yoshinaga, Joao Carlos Setubal, Sayuri Miyamoto, Ricardo Jose Giordano
Summary: By analyzing the lipid composition of the mouse retina, this study found that pathological angiogenesis leads to lipid remodeling and affects retinal homeostasis. The identified lipid signature could contribute to a better understanding of retinal diseases that cause vision impairment or blindness.
Article
Microbiology
Bruno C. Boettger, Carlos M. Piroupo, Joao C. Setubal, Raquel Girardello, Antonio C. C. Pignatari
Summary: This study describes a multidrug-resistant clinical isolate of Klebsiella pneumoniae carrying multiple resistance genes and plasmids of different types. This strain poses a potential threat to hospital-acquired infections.
CURRENT MICROBIOLOGY
(2023)
Article
Marine & Freshwater Biology
Marcele Laux, Carlos Morais Piroupo, Joao Carlos Setubal, Alessandra Giani
Summary: Two Raphidiopsis raciborskii metagenome-assembled genomes were recovered from Pampulha Lake in Brazil, and the analysis revealed differences in the presence of saxitoxin gene cluster and genome size between the two genomes. The study also found geographic origin to be a factor affecting the conservation level of essential gene repertoire, indicating distinct evolutionary history based on geographical isolation.
Article
Microbiology
Bianca Rodrigues Jardim, Lucy T. T. Tran-Nguyen, Cherie Gambley, Abdullah M. Al-Sadi, Ali M. Al-Subhi, Xavier Foissac, Pascal Salar, Hong Cai, Jun-Yi Yang, Richard Davis, Lynne Jones, Brendan Rodoni, Fiona E. Constable
Summary: Eight distinct species within the 16SrII group were identified using whole genome sequences, with six of them being newly described. These species have distinct biological and ecological features that can be differentiated by their ANI and AAI values. Additionally, the 16SrXXV- A taxon was classified as a new phytoplasma species.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Analia Carrau, Josefina Tano, Laura Moyano, Maria Belen Ripa, Silvana Petrocelli, Laura Piskulic, Leandro Marcio Moreira, Jose Salvatore Leister Patane, Joao Carlos Setubal, Elena Graciela Orellano
Summary: Plant-pathogen interaction is influenced by environmental factors such as temperature and light. This study shows that the bluf2 gene in Xcc bacterium regulates various aspects of virulence processes, including motility, adhesion, exopolysaccharide production, and biofilm formation. It also regulates ROS detoxification, which plays a role in the plant's response to infection. This is the first report on BLUF-like receptors in plant pathogenic bacteria.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2023)
Article
Entomology
Juliana Maria Conceicao Alves, Victor Hideki Nagatani, Otavio Guilherme Morais Silva, Manuela Oliveira Ramalho, Eduardo Goncalves Paterson Fox, Rodrigo Fernando de Souza, Debora Yumi Kayano, Alexandre Wagner Silva Hilsdorf, Ricardo Harakava, Maria Santina de Castro Morini
Summary: This study aims to evaluate the effect of human disturbances on the mitochondrial DNA haplotype diversity of S. saevissima ants in the Brazilian Atlantic Forest. The results show that the species exhibits high haplotypes and nucleotide diversity in different habitats, but all haplotypes seem closely related across habitats. Haplotype H1 is exclusively found in nests by highway roadsides, and haplotype H7 is found on dust roads; other haplotypes are recorded from all habitats. Overall, human activities have a significant impact on the genetic diversity of this species.
NEOTROPICAL ENTOMOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Joao Guilherme de Moraes Pontes, Hellen Bertoletti Barbieri, Alana Kelyene Pereira, Marina Ferreira Maximo, Aline Midori Kanashiro, Nelson Arno Wulff, Taicia Pacheco Fill
Summary: Huanglongbing (HLB) is a devastating citrus disease that causes significant economic damage and reduces the quality of oranges and juice. The bacteria Candidatus Liberibacter spp. is the likely cause of the disease, but no cure has been found. Current management strategies include using healthy nursery trees, controlling the psyllid vector, and eradicating symptomatic trees. Prolonged use of antibiotics, while effective, may have negative impacts on human health and the environment.
REVISTA VIRTUAL DE QUIMICA
(2023)
