Article
Cell Biology
Alexia Tiberi, Nicola Maria Carucci, Giovanna Testa, Caterina Rizzi, Paola Pacifico, Giulia Borgonovo, Ivan Arisi, Mara D'Onofrio, Rossella Brandi, Wen-Biao Gan, Simona Capsoni, Antonino Cattaneo
Summary: Nerve growth factor (NGF) has been shown to have a critical role in neuronal physiology. However, this study reveals that astrocytes in the central nervous system are also affected by changes in NGF levels, leading to astrocytic atrophy and proinflammatory responses. Additionally, inhibition of NGF leads to increased calcium activity in layer I astrocytes, which can be reversed by acute administration of NGF.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Shanshan Li, Yinquan Fang, Yihe Zhang, Mengmeng Song, Xilin Zhang, Xiao Ding, Hang Yao, Miaomiao Chen, Yiming Sun, Jianhua Ding, Qin Wang, Ming Lu, Guangyu Wu, Gang Hu
Summary: This study reveals the involvement of neurotoxic reactive astrocytes and microglial NLRP3 inflammasome in depression. Knockout of Nlrp3 in microglial cells alleviates the induction of A1-like astrocytes and improves neuronal dysfunction. The NF-KB pathway and caspase-1 activation play crucial roles in the production of A1-like astrocytes.
Review
Neurosciences
Aziz Unnisa, Nigel H. H. Greig, Mohammad Amjad Kamal
Summary: Traumatic brain injury (TBI) is a major cause of death and morbidity, and is the focus of translational research. Apoptosis is important in the pathophysiology of TBI, and inhibiting it may improve functional recovery. Caspases, such as Caspase-3 and Caspase-9, are key mediators of cell death due to apoptosis in TBI. Understanding these apoptotic pathways and finding ways to inhibit Caspases may have significant treatment implications for TBI.
CURRENT NEUROPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Vera Belousova, Oxana Svitich, Elena Timokhina, Irina Ignatko, Irina Bogomazova, Svetlana Pesegova, Tatiana Silaeva, Tatiana Kuzmina, Oxana Skorobogatova
Summary: This article presents the hypothesis that premature placenta apoptosis could be a potential cause of preterm birth, supported by evaluating gene expression. The study found that during pregnancy, the expression of apoptosis-related genes in the placenta is low, but the expression of the apoptosis inhibitor XIAP is high. However, at the onset of preterm labor, the expression of apoptosis-related genes increases significantly, potentially initiating preterm birth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Neurosciences
Karina Oyarce, Maria Yamila Cepeda, Raul Lagos, Camila Garrido, Ana Maria Vega-Letter, Maria Garcia-Robles, Patricia Luz-Crawford, Roberto Elizondo-Vega
Summary: This article reviews the effects of exosomes derived from glial cells on neurons and other glia in the context of neurodegeneration and neuroinflammation, as well as how specific stress stimuli and pathological conditions can alter exosome secretion levels and properties.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Francesca Anson, Pintu Kanjilal, S. Thayumanavan, Jeanne A. Hardy
Summary: Cytosolic protein delivery shows promise in various applications. However, the use of fluorescent tags can lead to undesired consequences which are often overlooked, making distinguishing between cytosolically released and endosomally entrapped proteins challenging. The development of a caspase-3 variant tagged with GFP aims to address this issue and facilitate the study of therapeutic properties and apoptotic cell death mechanisms.
Article
Biochemistry & Molecular Biology
Junhyung Kim, Jaejoon Lim, Ik Dong Yoo, Samel Park, Jong-Seok Moon
Summary: In this study, elevated TXNIP gene and protein levels were observed in the cerebral cortex of AD patients and AD mouse models. TXNIP was found to promote the expression of pro-inflammatory genes, cytokines and chemokines, as well as induce mitochondrial oxidative stress in human astrocytes. Furthermore, elevated TXNIP levels were associated with caspase-3 activation and apoptosis in GFAP-positive astrocytes of patients with AD and mouse AD.
Article
Biochemistry & Molecular Biology
Anhui Yang, Ping Zhang, Zhen Sun, Xin Liu, Xinrui Zhang, Xingkai Liu, Di Wang, Zhaoli Meng
Summary: The study demonstrated that lysionotin exhibited significant anti-liver cancer effects by potentially involving the caspase-3 mediated mitochondrial apoptosis pathway.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Cell Biology
Lujia Feng, Yantao Wei, Yimeng Sun, Linbin Zhou, Shaowei Bi, Weirong Chen, Wu Xiang
Summary: Cataracts are a leading cause of blindness worldwide, with age being a major risk factor. The exact mechanisms of cataractogenesis are still unclear. A recent study found that microRNA-34a (MIR34A) is involved in cataract development, but the underlying pathogenesis is unknown. This study showed that hexokinase 1 (HK1) is a target gene of MIR34A and demonstrated that MIR34A modulates lens epithelial cell apoptosis and cataract development through the HK1/caspase 3 signaling pathway.
Article
Biochemistry & Molecular Biology
Ming Zhang, Jianmei Lai, Qianlong Wu, Jia Lai, Jingyao Su, Bing Zhu, Yinghua Li
Summary: Hepatocarcinoma is a common digestive system tumor worldwide with no effective treatment. Naringenin, isolated from citrus fruits, has shown anticancer effects. This study investigated the molecular mechanisms and oxidative stress implications of naringenin-induced cytotoxicity in HepG2 cells. Naringenin induced apoptosis in HepG2 cells through multiple pathways, enhanced cytotoxic effects, triggered reactive oxygen species, inhibited JAK-2/STAT-3 signaling pathways, and activated caspase-3. These findings suggest that naringenin could be a promising candidate for cancer therapy.
Article
Geriatrics & Gerontology
Min-Huang Hsieh, Zhen-Yang Cui, Ai-Lun Yang, Nguyen Thanh Nhu, Shih-Ying Ting, Shao-Hong Yu, Yu-Jung Cheng, Yi-Yuan Lin, Xu-Bo Wu, Shin-Da Lee
Summary: The green tea flavonoid epigallocatechin-3-gallate (EGCG) may prevent neural apoptotic pathways and activate neural survival pathways in early aged hypertension-induced neural apoptosis.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Fisheries
Chang-Hong Cheng, Xiu-Ze Liu, Hong-Ling Ma, Guang-Xin Liu, Yi-Qin Deng, Juan Feng, Yu-Kun Jie, Zhi-Xun Guo
Summary: Sp-Caspase 3 plays important roles in the immune response and apoptosis against bacterial infection in the mud crab.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Cell Biology
Caroline M. S. Marques, Tatiana Pedron, Bruno L. Batista, Giselle Cerchiaro
Summary: The cellular prion protein (PrP(C)) plays an important role in cellular defense mechanisms and metal homeostasis in astrocytes cells, regulating apoptosis cell death and healthy growth.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Cell Biology
Donald H. Penning, Simona Cazacu, Aharon Brodie, Vesna Jevtovic-Todorovic, Steve N. Kalkanis, Michael Lewis, Chaya Brodie
Summary: The study found that ketamine induces apoptosis in neuronal and oligodendrocytic cells, and promotes the pro-inflammatory phenotypes of astrocytes and microglia. Astrocytes and microglia enhance the neurotoxic effects of ketamine on neuronal cells, while neurons increase oligodendrocyte cell death.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Food Science & Technology
Christiane Kruse Faeste, Anita Solhaug, Marion Gaborit, Florian Pierre, Dominique Massotte
Summary: Chronic exposure to the mycotoxin deoxynivalenol (DON) can have neurotoxic effects on brain cells, including impacts on glial cells, cell cycle, inflammatory cytokine release, and neuronal morphology.
Article
Biochemistry & Molecular Biology
Pei-Ling Chi, Chin-Chang Cheng, Cheng-Chung Hung, Mei-Tzu Wang, Hsien-Yueh Liu, Meng-Wei Ke, Min-Ci Shen, Kun-Chang Lin, Shu-Hung Kuo, Pin-Pen Hsieh, Shue-Ren Wann, Wei-Chun Huang
Summary: This study found that levels of MMP-1 and MMP-10 were increased in the vessel wall, serum, and M1-polarized macrophages of PAH patients and PAH rodent models. MMP-10 promotes the malignant phenotype of PASMCs by promoting their proliferation and migration. Inhibition of STAT1 can suppress hypoxia-induced MMP-10 expression in M1-polarized macrophages.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chien-Chung Yang, Li-Der Hsiao, Ya-Fang Shih, Zih-Yao Yu, Chuen-Mao Yang
Summary: This study demonstrates that rhamnetin attenuates the enhanced expression of MMP-9 induced by BK through inhibiting PKC delta/NOX/ROS/ERK1/2-dependent NF-kappa B activity in RBA-1 cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Chen-Yu Wang, Chien-Chung Yang, Li-Der Hsiao, Chuen-Mao Yang
Summary: This study investigated the expression and regulatory mechanisms of NRROS in astrocytes under the influence of 15d-PGJ(2). The results demonstrated that 15d-PGJ(2) induced NRROS expression through the PI3K/Akt-dependent phosphorylation of FoxO1 and Sp1, as well as the Nrf2 cascade, which effectively suppressed ROS generation and IL-6 expression.
NEUROTOXICITY RESEARCH
(2022)
Article
Anatomy & Morphology
Chih-Yang Hsu, Pei-Ling Chi, Hsin-Yu Chen, Shih-Hsiang Ou, Kang-Ju Chou, Hua-Chang Fang, Chien-Liang Chen, Chien-Wei Huang, Tzung-Yo Ho, Po-Tsang Lee
Summary: The study demonstrates successful regeneration of kidneys through seeding cells on acellular scaffolds, showing functional abilities to produce urine and reabsorb substances. This research holds promise in addressing organ shortage issues and providing therapeutic solutions for patients.
Article
Cell Biology
Chien-Chung Yang, Li-Der Hsiao, Ya-Fang Shih, Chih-Kai Hsu, Chia-Yu Hu, Chuen-Mao Yang
Summary: Thrombin triggers the upregulation of COX-2 to promote the secretion of PGE(2), which involves the PAR1/G(q) or G(i/o)/PKCα/MAPK-dependent activation of NF-κB in HTSMCs.
MEDIATORS OF INFLAMMATION
(2022)
Article
Cell Biology
Chien-Chung Yang, Li-Der Hsiao, Ya-Fang Shih, Hsin-Hui Lin, Chuen-Mao Yang
Summary: This study demonstrated that DDF induced upregulation of HO-1 expression via the EGFR/PKCα/PI3K/Akt-dependent Sp1 pathway in HCFs, leading to attenuation of inflammation responses.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Cell Biology
Chien-Chung Yang, Li-Der Hsiao, Ya-Fang Shih, Mei-Hsiu Su, Chuen-Mao Yang
Summary: This study explores the comprehensive mechanisms of how S1P promotes the expression of COX-2 and PGE(2) and induces apoptosis in human cardiac fibroblasts (HCFs) through the activation of MMP9/EGFR-mediated PI3K/Akt/MAPKs-dependent AP-1 activity.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Review
Biochemistry & Molecular Biology
Yi-Hsuan Wu, Hsi-Lung Hsieh
Summary: The heme oxygenase system, particularly HO-1 and HO-2, plays an important role in the nervous system. Dysregulation of HO-1 is associated with neurodegenerative diseases.
Article
Biochemistry & Molecular Biology
Chien-Chung Yang, Li-Der Hsiao, Ya-Fang Shih, Ching- Chang, Chuen-Mao Yang
Summary: 15d-PGJ(2) induces the expression of HO-1 in bEnd.3 cells through the activation of NOX- and mitochondria-derived ROS-dependent PKC delta/JNK1/2/Sp1/AP-1 signaling pathway, thus protecting against inflammatory responses.
Article
Biochemistry & Molecular Biology
Chien-Chung Yang, Li-Der Hsiao, Chen-Yu Wang, Wei-Ning Lin, Ya-Fang Shih, Yi-Wen Chen, Rou-Ling Cho, Hui-Ching Tseng, Chuen-Mao Yang
Summary: This study demonstrates that kaempferol (KPR) can induce HO-1 expression and attenuate inflammation in pulmonary alveolar epithelial cells (HPAEpiCs) through the activation of NOX/ROS/c-Src/Pyk2/PKCα/p38α MAPK and JNK1/2 pathways. The findings suggest that KPR could be a potential strategy for protecting against pulmonary inflammation.
Article
Biochemistry & Molecular Biology
Ming-Ming Tsai, Jiun-Liang Chen, Tsong-Hai Lee, Hsiuming Liu, Velayuthaprabhu Shanmugam, Hsi-Lung Hsieh
Summary: Resveratrol can inhibit the expression of matrix metalloproteinase-9 (MMP-9) induced by IL-1 beta, reducing the disruption of tight junctions in brain microvascular endothelial cells caused by inflammation.
Article
Biochemistry & Molecular Biology
Chun-Han Su, Yu-Chieh Cheng, Yu-Chia Chang, Ting-Hsuan Kung, Yu-Li Chen, Kuei-Hung Lai, Hsi-Lung Hsieh, Chun-Yu Chen, Tsong-Long Hwang, Yu-Liang Yang
Summary: This study examined the antiproliferative activity of different extracts of Tetradium ruticarpum (TR) fruit and highlighted potentially antiproliferative compounds using an untargeted LC-MS/MS-based multi-informative molecular networking strategy. The EtOAc fraction F2-3 exhibited the most potent antiproliferative activity against human cell lines. Through computational tool-aided structure prediction and integration of various data, 11 indole alkaloids and 47 types of quinolone alkaloids were successfully annotated and visualized into three targeted bioactive molecular families. Several previously unreported quinolone alkaloids in TR fruit were discovered. Four indole alkaloids and five types of quinolone alkaloids were identified as potentially antiproliferative compounds in the EtOAc fraction F2-3. Evodiamine was confirmed as a major antiproliferative compound, and its structural analogues found in the molecular network showed promise as antitumor agents. This study exemplifies the application of an LC-MS/MS-based multi-informative molecular networking strategy in the discovery and annotation of bioactive compounds from complex mixtures of potential functional food ingredients.
Article
Biochemistry & Molecular Biology
Chien-Chung Yang, I-Ta Lee, Yan-Jyun Lin, Wen-Bin Wu, Li-Der Hsiao, Chuen-Mao Yang
Summary: This study confirmed that thrombin induces the production of COX-2 and PGE(2) in human tracheal smooth muscle cells, leading to inflammation in the airways and lungs. The study identified specific signaling pathways involved, including the PKC delta/Pyk2/c-Src/EGFR/PI3K/Akt/AP-1 pathway. Resveratrol was also found to inhibit COX-2 and PGE(2) production by blocking the activation of these signaling molecules, suggesting its potential use in managing airway inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Shue-Ren Wann, Horng-Ren Lo, Yun-Te Chang, Jia-Bin Liao, Zhi-Hong Wen, Pei-Ling Chi
Summary: Vibrio vulnificus infection induces hyperinflammatory and cytotoxic inflammation, with P2X7R playing a crucial role in macrophages and providing new therapeutic opportunities in bacterial infections.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Yi-Hsuan Wu, Hsi-Lung Hsieh
Summary: Bioenergetic mitochondrial dysfunction is a common feature of various diseases, including Alzheimer's disease. Redox imbalance and mitochondrial dysfunction play important roles in the development of Alzheimer's disease. This review summarizes the evidence for redox imbalance and mitochondrial dysfunction in Alzheimer's disease, and discusses current therapeutic strategies related to mitochondrial maintenance.