Article
Biotechnology & Applied Microbiology
Yuzheng Wu, Honami Kawabata, Kyosuke Kita, Shu Ishikawa, Kan Tanaka, Ken-ichi Yoshida
Summary: In this study, artificial manipulation of NADPH regeneration in Bacillus subtilis was found to enhance the conversion of myo-inositol. Luciferase was also identified as a potential indicator of intracellular NADPH levels.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Yaqing Zhu, Linlin Wang, Kaixuan Zheng, Ping Liu, Wenkang Li, Jian Lin, Wenjing Liu, Shoushui Shan, Liqin Sun, Hailing Zhang
Summary: Collagenase was successfully recombinantly expressed in Bacillus subtilis and its properties were characterized. The activity of the recombinant collagenase was significantly improved by optimizing the culture conditions.
FERMENTATION-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Luyi Cheng, Elise N. White, Naomi L. Brandt, Angela M. Yu, Alan A. Chen, Julius B. Lucks
Summary: The study investigates the structure and function of the Bacillus subtilis yxjA purine riboswitch and suggests a potential mechanism whereby ligand binding induces conformational changes in RNA structures. The results provide insight into how RNA functions in gene regulation.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Aisling Brady, Nuria Quiles-Puchalt, Francisca Gallego del Sol, Sara Zamora-Caballero, Alonso Felipe-Ruiz, Jorge Val-Calvo, Wilfried J. J. Meijer, Alberto Marina, Jose R. Penades
Summary: Some Bacillus-infecting bacteriophages utilize a peptide-based communication system, called arbitrium, to coordinate the lysis-lysogeny decision. AimR, a key player in this system, is involved in a complex network of regulation. The SP beta phage, possessing a functional arbitrium system, can optimize production of infective particles while preserving the number of surviving cells, which increases phage persistence in nature.
Article
Microbiology
Xiaoying Ouyang, Yingjie Liu, Ruidan Qu, Min Tian, Ting Yang, Rui Zhu, Hongliang Gao, Mingfei Jin, Jing Huang
Summary: PG, a protein deaminase derived from C. proteolyticum, shows promising potential for application in the food industry. Different strategies for PG expression in B. subtilis and its enzymatic properties provide efficient alternatives for PG research and contribute to the industrial-scale production of PG.
CURRENT MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Silja Vaelimets, Patricia Pedetti, Ludovika Jessica Virginia, Mai Ngoc Hoang, Michael Sauer, Clemens Peterbauer
Summary: Small laccases from Streptomyces coelicolor, Streptomyces viridosporus, and Amycolatopsis 75iv2 were successfully expressed in both Gram-positive expression hosts B. subtilis and S. lividans. However, the extracellular activities of the laccases were significantly higher in S. lividans compared to B. subtilis.
MICROBIAL CELL FACTORIES
(2023)
Article
Mathematics, Applied
Jianyu Hu, Xiaoli Chen, Jinqiao Duan
Summary: We investigate the quantitative network of gene expression dynamics in the competence development of Bacillus subtilis, quantifying the most probable transition pathways for both excitable and bistable dynamics and analyzing how noise intensity affects the transition phenomena.
Article
Biochemical Research Methods
Kristoffer Bach Falkenberg, Vivienne Mol, Arrate Sainz de la Maza Larrea, Ivan Pogrebnyakov, Morten H. H. Norholm, Alex Toftgaard Nielsen, Sheila Ingemann Jensen
Summary: Versatile DNA assembly standards and well-characterized part libraries are crucial tools in synthetic biology. Despite limitations in vector standards for Gram-positive hosts, the ProUSER2.0 toolbox provides a standardized platform for creating shuttle vectors in Bacillus subtilis. This system is semi-compatible with previously developed standards and allows for efficient insertion of cargo sequences. Through thorough characterization of integration sites and promoters, the toolbox facilitates the construction of signal peptide libraries for industrial proteins. Overall, the ProUSER2.0 toolbox is a powerful and flexible framework for use in B. subtilis.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Food Science & Technology
Yi-Zhou Xiang, Xin-Yue Li, Hai-Long Zheng, Jun-Yuan Chen, Lian-Bing Lin, Qi-Lin Zhang
Summary: The novel bacteriocin JS17 obtained from Bacillus subtilis isolated from blueberry ferments showed extensive antibacterial activity, heat tolerance, and high stability over pH 2-10, making it a potential food bio-preservative. Additionally, JS17 exhibited lower minimum inhibitory and bactericidal concentrations against Escherichia coli compared to most known bacteriocins.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Veterinary Sciences
Qiuju Wang, Yulong Ren, Yizhe Cui, Bingnan Gao, Hao Zhang, Qianming Jiang, Juan J. Loor, Zhaoju Deng, Chuang Xu
Summary: This study investigated the endosymbiotic links between Bacillus subtilis and ruminal tissue, and found that active bacteria can promote protein synthesis in ruminal tissue explants.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agricultural Engineering
Xuyang Zhu, Kang Zhang, Hui Luo, Jing Wu
Summary: The bottleneck of recombinant protein production in microbial cell factories is sometimes caused by limited manipulable targets and a lack of gene annotation related to protein expression. In this study, the novel functions of PonA in Bacillus subtilis recombinant protein expression were described, and the mechanism of its chaperone activity was analyzed. Overexpression of PonA led to a significant increase in the expression of hyperthermophilic amylase in shake flasks and fed-batch processes. Increased cell diameter and reinforced cell walls were observed in PonA-overexpressing strains. The FN3 structural domain and the natural dimeric structure of PonA may play critical roles in its chaperone activity. These findings suggest that PonA can be an effective target for modifying the expression of recombinant proteins in B. subtilis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Microbiology
Jeremy Garb, Anna Lopatina, Aude Bernheim, Mindaugas Zaremba, Virginijus Siksnys, Sarah Melamed, Azita Leavitt, Adi Millman, Gil Amitai, Rotem Sorek
Summary: DSR proteins defend bacteria from phage infection by degrading NAD(+) and acting as active NADases, with DSR2 directly identifying phage proteins. Some phages express anti-DSR2 proteins to block bacterial immunity. This study establishes the general role of SIR2 domains in bacterial immunity against phages.
NATURE MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Luyao Bao, Juan Hu, Bowen Zhan, Mingzhe Chi, Zhengyang Li, Sen Wang, Chan Shan, Zhaozhao Zhao, Yanchao Guo, Xiaoming Ding, Chaoneng Ji, Shengce Tao, Ting Ni, Xuelian Zhang, Guoping Zhao, Jixi Li
Summary: This study reports the crystal structures of RNase J from Mycobacterium tuberculosis in apo-state and complex with single strand RNA, revealing its physiological function in RNA metabolism.
NATURE COMMUNICATIONS
(2023)
Article
Food Science & Technology
Meng Zhao, Duanmu Liu, Zhihong Liang, Kunlun Huang, Xiaoyan Wu
Summary: The proteins produced by Bacillus subtilis CW14 and β-glucanase can effectively inhibit Aspergillus ochraceus, increase OTA production, and may affect the secondary metabolism of Aspergillus ochraceus by activating the CWI signaling pathway.
Article
Chemistry, Applied
Fen Yan, Xiaoling Ye, Chunhua Li, Peisong Wang, Shuqiong Chen, Hetong Lin
Summary: The antifungal protein MG-3A was isolated, purified, and identified, showing broad antifungal spectrum and good stabilities to temperature, pH and protease K. The serine protease gene MG-3A was cloned and expressed in vitro, with highly soluble recombinant protein. Affinity chromatography was used to purify the recombinant protein and evaluate its antifungal activity.
Article
Multidisciplinary Sciences
Karl A. Hassan, Varsha Naidu, Jacob R. Edgerton, Karla A. Mettrick, Qi Liu, Leila Fahmy, Liping Li, Scott M. Jackson, Irshad Ahmad, David Sharples, Peter J. F. Henderson, Ian T. Paulsen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Infectious Diseases
Muhammad Shafee, Ferhat Abbas, Zunera Tanveer, Andrew Whitelaw, Lemese Ah Tow, Muhammad Ashraf, Irshad Ahmad, Simon G. Patching, Abdul Jabbar, Ali Akbar, Mohammad Zahid Mustafa
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2019)
Article
Biology
Roshan Ali, Yuannian Jiao, P. Kerr Wall, Simon G. Patching, Irshad Ahmad, Ghosia Lutfulla, Claude W. dePamphilis
COMPUTATIONAL BIOLOGY AND CHEMISTRY
(2020)
Review
Chemistry, Multidisciplinary
Peter J. F. Henderson, Claire Maher, Liam D. H. Elbourne, Bart A. Eijkelkamp, Ian T. Paulsen, Karl A. Hassan
Summary: Bacterial multidrug efflux pumps play a crucial role in protecting bacteria from antimicrobials, but they also have other physiological functions. Studying the true physiological roles of these efflux pumps can help design more effective drugs and improve the efficiency of microbial drug production and commercial development.
Article
Food Science & Technology
Roshina Rabail, Javeria Saleem, Zunera Tanveer, Simon G. Patching, Abdur Rauf Khalid, Muhammad Tauseef Sultan, Muhammad Faisal Manzoor, Emad Karrar, Muhammad Inam-Ur-Raheem, Muhammad Asim Shabbir, Rana Muhammad Aadil
Summary: The study found that factors such as being male, aged over 40, insufficient sleep, lack of physical activity, obesity, diabetes mellitus, and autoimmune diseases were significantly associated with delayed recovery from COVID-19. Poor nutritional outcomes, such as lower intake of water, legumes, nuts, meat, and milk/yogurt; and higher consumption of fast/fried/junk/spicy foods and cold water/drinks, were also significantly linked to a longer recovery period.
FOOD SCIENCE & NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Hannah M. Southam, Michael P. Williamson, Jonathan A. Chapman, Rhiannon L. Lyon, Clare R. Trevitt, Peter J. F. Henderson, Robert K. Poole
Summary: CORMs are molecules used to release carbon monoxide, showing antibacterial activity while being benign to mammalian cells. CORM-2 exhibits potent antibacterial activity, possibly linked to ruthenium toxicity, and exogenous amino acids and thiols can protect bacteria from the effects of CORM-2.
Review
Microbiology
Karl A. Hassan, Claire Maher, Liam D. H. Elbourne, Peter J. F. Henderson, Ian T. Paulsen
Summary: The release of genome sequences for thousands of bacterial species since the late 1990s has led to the discovery of numerous uncharacterised genes encoding putative membrane proteins. Using transcriptomics and detailed biochemical analysis, researchers have identified a novel family of multidrug efflux pumps and confirmed their functions. This general functional genomics approach holds promise for future research in identifying more transport proteins of scientific, clinical, and commercial interest.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Irshad Ahmad, Sonia Khattak, Roshan Ali, Nighat Nawaz, Kalim Ullah, Sher Bahadar Khan, Mumtaz Ali, Simon G. Patching, Mohammad Zahid Mustafa
Summary: This study found that raw dairy milk samples in the Peshawar region of Pakistan were contaminated with E. coli, with some samples also containing the pathogenic O157:H7 serotype, which showed high resistance to multiple antibiotics. This poses a significant public health risk and calls for enhanced surveillance, hygiene practices in milk production and storage, and educational programs for farmers and workers.
JOURNAL OF FOOD SAFETY
(2021)
Review
Microbiology
Sahaya Nadar, Tabassum Khan, Simon G. Patching, Abdelwahab Omri
Summary: This review discusses the formation of biofilms and the strategies and mechanisms of antibiofilm agents. It emphasizes the importance of using multiple approaches and combination therapy to enhance the control and management of biofilms.
Article
Biochemistry & Molecular Biology
Saroj Basnet, Rishab Marahatha, Asmita Shrestha, Salyan Bhattarai, Saurav Katuwal, Khaga Raj Sharma, Bishnu P. Marasini, Salik Ram Dahal, Ram Chandra Basnyat, Simon G. Patching, Niranjan Parajuli
Summary: This study identifies cordifolioside-A from T. cordifolia as a potential candidate for combating SARS-CoV-2 based on in vitro screening and in silico studies.
Article
Pharmacology & Pharmacy
Irshad Ahmad, Muhammad Ali, Roshan Ali, Nighat Nawaz, Simon G. Patching
Summary: This study used a structure-based virtual screening and molecular docking approach to identify existing drugs that may have the potential to inhibit the SARS-CoV-2 virus. The highest affinity drugs identified were Tolvaptan and Nizoral.
PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Correction
Biochemistry & Molecular Biology
M. Saidijam, S. G. Patching
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2020)
Article
Chemistry, Multidisciplinary
Nighat Nawaz, Irshad Ahmad, Nizam M. Darwesh, Amjad Wahab, Sadeeq ur Rahman, Abdul Sajid, Farhan A. Khan, Sher B. Khan, Simon G. Patching, Kamal Uddin
JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN
(2020)
Article
Biotechnology & Applied Microbiology
Abdul Samad, Ferhat Abbas, Zunera Tanveer, Zafar Ahmad, Irshad Ahmad, Simon G. Patching, Nighat Nawaz, Muhammad Tauseef Asmat, Abdul Raziq, Asadullah, Irfan Shahzad Sheikh, Muhammad Naeem, Olena Pokryshko, Mohammad Zahid Mustafa
ROMANIAN BIOTECHNOLOGICAL LETTERS
(2019)