Review
Pharmacology & Pharmacy
Kawthar Dhayni, Kazem Zibara, Hawra Issa, Said Kamel, Youssef Bennis
Summary: CXCR1 and CXCR2 chemokine receptors are critical in inflammation, and CXCR1/2 inhibitors show beneficial effects in preventing cardiovascular disease progression.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Oncology
Dipakkumar R. Prajapati, Caitlin Molczyk, Abhilasha Purohit, Sugandha Saxena, Reegan Sturgeon, Bhavana J. Dave, Sushil Kumar, Surinder K. Batra, Rakesh K. Singh
Summary: Pancreatic cancer (PC) has a poor prognosis, and targeting CXCR2/1 using small molecule inhibitors shows promise in inhibiting PC growth, angiogenesis, and metastasis. In this study, the therapeutic utility of the CXCR2/1 antagonist SCH-479833 was evaluated in different PC murine models, demonstrating antitumor and anti-metastatic effects. CXCR2/1 antagonist treatment inhibited tumor cell proliferation, migration, angiogenesis, and neutrophil recruitment, while promoting apoptosis, fibrosis, tumor necrosis, and extramedullary hematopoiesis. These findings suggest that selectively targeting CXCR2/1 with small molecule inhibitors is a promising therapeutic approach for PC.
Article
Cell & Tissue Engineering
Adeline Blandinieres, Xuechong Hong, Aurelien Philippe, Ivan Bieche, Sophie Vacher, Elisa Rossi, Gregoire Detriche, Nicolas Gendron, Pascale Gaussem, Coralie L. Guerin, Juan M. Melero-Martin, David M. Smadja
Summary: Studies have investigated the presence of CXCR1 and CXCR2 receptors on ECFCs, finding that these receptors were not detected. In addition, comparison of RT-PCR primers used to search for CXCR1 and CXCR2 mRNA revealed potential non-specific DNA amplification.
STEM CELL REVIEWS AND REPORTS
(2021)
Article
Oncology
Paola Verachi, Francesca Gobbo, Fabrizio Martelli, Andrea Martinelli, Giuseppe Sarli, Andrew Dunbar, Ross L. Levine, Ronald Hoffman, Maria Teresa Massucci, Laura Brandolini, Cristina Giorgio, Marcello Allegretti, Anna Rita Migliaccio
Summary: This study demonstrates that Reparixin treatment can reduce fibrosis in a mouse model of myelofibrosis by decreasing the expression of TGF-beta 1 and collagen III while increasing the expression of GATA1. These findings provide a preclinical rationale for further evaluating the use of Reparixin in the treatment of patients with myelofibrosis.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Simrit Safarulla, Ankit Madan, Fei Xing, Arvind Chandrasekaran
Summary: This study reveals the mechanisms by which CXCR2 affects neutrophils in brain metastasis of breast cancer. Activation of CXCR2 receptors by brain metastatic tumors promotes neutrophil migration to tumor sites and formation of NETs, thereby facilitating tumor progression. Inhibiting CXCR2 and NETs formation can limit the interaction between neutrophils and tumors, slowing down the development of brain metastatic breast cancer.
Article
Oncology
Geny Piro, Carmine Carbone, Antonio Agostini, Annachiara Esposito, Maria De Pizzol, Rubina Novelli, Marcello Allegretti, Andrea Aramini, Alessia Caggiano, Alessia Granitto, Francesco De Sanctis, Stefano Ugel, Vincenzo Corbo, Maurizio Martini, Rita Teresa Lawlor, Aldo Scarpa, Giampaolo Tortora
Summary: This study evaluated the effectiveness of combining a CXCR1/2 inhibitor with anti-PD-1 in treating immunosuppression in PDAC. The results showed that this combination therapy can reverse M2 macrophage polarization, reduce tumor burden, and improve the efficacy of immunotherapy.
BRITISH JOURNAL OF CANCER
(2023)
Article
Immunology
Alvaro Teijeira, Saray Garasa, Maria del Carmen Ochoa, Assunta Cirella, Irene Olivera, Javier Glez-Vaz, Maria Pilar Andueza, Itziar Migueliz, Maite Alvarez, Maria Esperanza Rodriguez-Ruiz, Ana Rouzaut, Pedro Berraondo, Miguel F. Sanmamed, Jose L. Perez Gracia, Ignacio Melero
Summary: IL-8 plays a key role in guiding neutrophils and monocytes/macrophages through chemotaxis; IL-8-induced NETosis is less dependent on G-proteins and can be inhibited by extracellular Ca+2 chelation; NETosis can be more readily inhibited with anti-CXCR1 antibody than chemotaxis.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Chemistry, Physical
Haiyan Yang, Zheyuan Shen, Linxiang Luo, Jian Gao, Sikang Chen, Jinxin Che, Lei Xu, Meijuan Wu, Xiaowu Dong
Summary: CXC chemokine receptors 1 (CXCR1) and 2 (CXCR2), members of the GPCRs family, play important roles in cancer cell proliferation, apoptosis, and angiogenesis. The high sequence homology between CXCR1 and CXCR2 poses a challenge in developing selective antagonists. Through computational methods, it was discovered that the differences in the amino acids ASN311 on CXCR1 and LYS320 on CXCR2 are the main reason for the different antagonistic abilities of CXCR1/2, providing important clues for the development of selective CXCR1/2 antagonists.
CHEMICAL PHYSICS LETTERS
(2022)
Article
Immunology
Stefano Bagatella, Neda Haghayegh Jahromi, Camille Monney, Margherita Polidori, Flavio Max Gall, Emma Marchionatti, Fabienne Serra, Rainer Riedl, Britta Engelhardt, Anna Oevermann
Summary: This study investigates the chemotaxis of bovine polymorphonuclear neutrophils (PMN) towards Listeria monocytogenes (Lm) and infected brain cell populations. The results show that microglia have a primary role in initiating PMN chemotaxis towards Lm in the brain during neurolisteriosis, and blockade of the factors involved in this process could be a potential therapeutic target.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Fisheries
Zihao Zhao, Hao Peng, Tao Han, Zhijing Jiang, Jieyi Yuan, Xue Liu, Xiaoqian Wang, Yuexing Zhang, Tianming Wang
Summary: From transcriptomic data of largemouth bass, an IL8R highly homologous to teleost IL-8Rs was identified. Phylogenetic analysis compared M. salmoides IL-8 receptor (MsCXCR2) with the C-X-C motif chemokine receptor 2 subfamily. Results showed activation of MsCXCR2 by LcIL-8, internalization of the receptor, and ERK phosphorylation. Constitutive expression of MsCXCR2 in examined tissues, with significant upregulation in the liver and head kidney of diseased fish, was observed.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Vincent Yi-Fong Su, Wei-Chih Chen, Wen-Kuang Yu, Huai-Hsuan Wu, Hao Chen, Kuang-Yao Yang
Summary: Nintedanib showed significant reduction in histopathological changes and neutrophil recruitment in LPS-induced ALI. It mediated the downregulation of adhesion molecules, decreased the phosphorylated p38:total p38 ratio, and improved neutrophil chemotaxis by reducing CXCR2 expression and activating GRK2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Li-Pang Chuang, Huang-Pin Wu, Li-Ang Lee, Li-Chung Chiu, Shih-Wei Lin, Han-Chung Hu, Kuo-Chin Kao, Ning-Hung Chen, Jung-Wei Tsai, Jong-Hwei Su Pang
Summary: This study found that under intermittent hypoxia conditions, the expression of IL-8 was enhanced in monocytes of adult OSA patients, leading to an increase in the migration ability of monocytes towards IL-8.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Gastroenterology & Hepatology
Jack Leslie, John B. G. Mackey, Thomas Jamieson, Erik Ramon-Gil, Thomas M. Drake, Frederic Fercoq, William Clark, Kathryn Gilroy, Ann Hedley, Colin Nixon, Saimir Luli, Maja Laszczewska, Roser Pinyol, Roger Esteban-Fabro, Catherine E. Willoughby, Philipp K. Haber, Carmen Andreu-Oller, Mohammad Rahbari, Chaofan Fan, Dominik Pfister, Shreya Raman, Niall Wilson, Miryam Muller, Amy Collins, Daniel Geh, Andrew Fuller, David McDonald, Gillian Hulme, Andrew Filby, Xabier Cortes-Lavaud, Noha-Ehssan Mohamed, Catriona A. Ford, Ximena L. Raffo Iraolagoitia, Amanda J. McFarlane, Misti McCain, Rachel A. Ridgway, Edward W. Roberts, Simon T. Barry, Gerard J. Graham, Mathias Heikenwalder, Helen L. Reeves, Josep M. Llovet, Leo M. Carlin, Thomas G. Bird, Owen J. Sansom, Derek A. Mann
Summary: This study found that targeting neutrophils using a CXCR2 small molecule inhibitor can enhance the sensitivity of non-alcoholic steatohepatitis-associated hepatocellular carcinoma (NASH-HCC) to immune checkpoint inhibition therapy (ICI). The combination therapy can reprogram the tumor immune microenvironment, increase the number of anti-tumor immune cells, and improve treatment outcomes.
Article
Infectious Diseases
Phillip M. G. Peixoto, Lais L. Cunha, Leonardo Barbosa, Wilson Coelho, Giorgia Podico, Rodrigo C. Bicalho, Igor F. Canisso, Fabio S. Lima
Summary: This study aimed to evaluate the effectiveness of recombinant bovine interleukin-8 (rbIL-8) in improving neutrophil function and reducing high somatic cell count (SCC) in cows. The results showed that intramammary infusion of rbIL-8 did not significantly improve neutrophil response or resolve high SCC.
Review
Oncology
Jan Korbecki, Patrycja Kupnicka, Katarzyna Barczak, Mateusz Bosiacki, Pawel Zietek, Dariusz Chlubek, Irena Baranowska-Bosiacka
Summary: AML is a type of leukemia with a poor prognosis. This review focuses on the significance of CXC chemokines other than CXCL12 in AML, including their roles in AML tumor processes, clinical relevance, and association with other factors such as chemoresistance and bone marrow angiogenesis. The aim of this study is to provide an overview of the less-studied CXC chemokine axes in AML and bridge the gap in current research.
Article
Infectious Diseases
P. Rainard, G. Foucras, J. R. Fitzgerald, J. L. Watts, G. Koop, J. R. Middleton
TRANSBOUNDARY AND EMERGING DISEASES
(2018)
Review
Agriculture, Dairy & Animal Science
P. Rainard, G. Foucras, D. Boichard, R. Rupp
JOURNAL OF DAIRY SCIENCE
(2018)
Article
Microbiology
Shlomo E. Blum, Robert J. Goldstone, James P. R. Connolly, Maryline Reperant-Ferter, Pierre Germon, Neil F. Inglis, Oleg Krifucks, Shubham Mathur, Erin Manson, Kevin Mclean, Pascal Rainard, Andrew J. Roe, Gabriel Leitner, David G. E. Smith
Article
Multidisciplinary Sciences
Pascal Rainard, Maryline Reperant-Ferter, Christophe Gitton, Florence B. Gilbert, Pierre Germon
Article
Multidisciplinary Sciences
Vincent Herry, Christophe Gitton, Guillaume Tabouret, Maryline Reperant, Laurine Forge, Christian Tasca, Florence B. Gilbert, Edouard Guitton, Celine Barc, Christophe Staub, David G. E. Smith, Pierre Germon, Gilles Foucras, Pascal Rainard
SCIENTIFIC REPORTS
(2017)
Article
Agriculture, Dairy & Animal Science
R. Rupp, C. Huau, H. Caillat, T. Fassier, F. Bouvier, E. Pampouille, V Clement, I Palhiere, H. Larroque, G. Tosser-Klopp, P. Jacquiet, P. Rainard
JOURNAL OF DAIRY SCIENCE
(2019)
Article
Multidisciplinary Sciences
Patricia Cunha, Yves Le Vern, Christophe Gitton, Pierre Germon, Gilles Foucras, Pascal Rainard
SCIENTIFIC REPORTS
(2019)
Article
Immunology
Nathan Cebron, Sarah Maman, Sarah Walachowski, Blandine Gausseres, Patricia Cunha, Pascal Rainard, Gilles Foucras
Review
Agriculture, Dairy & Animal Science
Pascal Rainard, Florence B. Gilbert, Pierre Germon, Gilles Foucras
Summary: Infections of the mammary gland are a common disease in dairy ruminants that have negative effects on animal welfare and milk quality. Despite decades of research, the development of mastitis vaccines has not fully met the demand, mainly due to the lack of complete understanding of the immune mechanisms for effective protection.
JOURNAL OF DAIRY SCIENCE
(2021)
Review
Immunology
Pascal Rainard, Florence B. Gilbert, Rodrigo P. Martins, Pierre Germon, Gilles Foucras
Summary: Mastitis is a major problem in dairy farming, and the current vaccines are not effective. To address this issue, research should focus on the immunobiology of the mammary gland and the pathogens adapted to it. Vaccines need to elicit immune mechanisms different from those induced by infection, in order to be more effective.
Letter
Microbiology
Pascal Rainard
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Veterinary Sciences
Pascal Rainard, Patricia Cunha, Rodrigo P. Martins, Florence B. Gilbert, Pierre Germon, Gilles Foucras
VETERINARY RESEARCH
(2020)
Article
Veterinary Sciences
Pascal Rainard, Christophe Gitton, Thierry Chaumeil, Thierry Fassier, Christophe Huau, Mickael Riou, Gwenola Tosser-Klopp, Zuzana Krupova, Anne Chaize, Florence B. Gilbert, Rachel Rupp, Patrice Martin
VETERINARY RESEARCH
(2018)
Article
Veterinary Sciences
Katharina Sobotta, Katharina Bonkowski, Elisabeth Liebler-Tenorio, Pierre Germon, Pascal Rainard, Nina Hambruch, Christiane Pfarrer, Ilse D. Jacobsen, Christian Menge
VETERINARY RESEARCH
(2017)
Review
Veterinary Sciences
Pascal Rainard, Gilles Foucras
FRONTIERS IN VETERINARY SCIENCE
(2018)