Article
Chemistry, Multidisciplinary
Luca Nagel, Martin Gierse, Wolfgang Gottwald, Zumrud Ahmadova, Martin Grashei, Pascal Wolff, Felix Josten, Senay Karaali, Christoph A. Mueller, Sebastian Lucas, Jochen Scheuer, Christoph Mueller, John Blanchard, Geoffrey J. Topping, Andre Wendlinger, Nadine Setzer, Sandra Suehnel, Jonas Handwerker, Christophoros Vassiliou, Frits H. A. van Heijster, Stephan Knecht, Michael Keim, Franz Schilling, Ilai Schwartz
Summary: Hyperpolarization techniques significantly increase nuclear spin polarization, enabling metabolic MRI. However, the complexity, cost, and slowness of current equipment limit its widespread use. In this study, a polarization procedure using parahydrogen-induced polarization by side-arm hydrogenation (PHIP-SAH) in an automated polarizer is demonstrated, which produces purified, concentrated, and highly hyperpolarized pyruvate solutions within a short time. The results show that PHIP is a viable alternative to established hyperpolarization techniques, with equivalent safety profile, image quality, and quantitative measurements.
Article
Chemistry, Multidisciplinary
J. Eills, E. Cavallari, R. Kircher, G. Di Matteo, C. Carrera, L. Dagys, M. H. Levitt, K. L. Ivanov, S. Aime, F. Reineri, K. Muennemann, D. Budker, G. Buntkowsky, S. Knecht
Summary: This study introduces a novel H-1 imaging method in which hyperpolarized spin order is released for imaging through a specific biochemical reaction, while suppressing background signals from water. It allows for hyperpolarized imaging without the need for low-abundance, low-sensitivity heteronuclei.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Cardiac & Cardiovascular Systems
Fawaz Alenezi, Taylor A. Covington, Monica Mukherjee, Stephen C. Mathai, Paul B. Yu, Sudarshan Rajagopal
Summary: There is growing recognition of the significance of the right heart and pulmonary circulation in various disease states related to pulmonary hypertension and left heart failure. However, assessing the structure and function of the right heart and pulmonary circulation can be challenging due to complex factors such as the shape of the right ventricle and comorbidities. New imaging techniques show promise in providing more precise diagnostic information. These technologies have the potential to improve early diagnosis and noninvasive monitoring, leading to better patient care.
CIRCULATION RESEARCH
(2022)
Review
Chemistry, Multidisciplinary
Nikita V. Chukanov, Roman V. Shchepin, Sameer M. Joshi, Mohammad S. H. Kabir, Oleg G. Salnikov, Alexandra Svyatova, Igor V. Koptyug, Juri G. Gelovani, Eduard Y. Chekmenev
Summary: NMR hyperpolarization techniques greatly increase nuclear spin polarization, leading to significant sensitivity gains for applications in high-resolution NMR spectroscopy and real-time metabolic imaging. Various hyperpolarization techniques exist, with a focus on targeting C-13 and N-15 sites in biocompatible agents. Long T-1 values of these sites allow hyperpolarized states to be retained for extended periods, with SABRE being a versatile low-cost technique for hyperpolarization of N-15 nuclei.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Patrick TomHon, Mustapha Abdulmojeed, Isaiah Adelabu, Shiraz Nantogma, Mohammad Shah Hafez Kabir, Soren Lehmkuhl, Eduard Y. Chekmenev, Thomas Theis
Summary: This study introduces a temperature cycling method for SABRE-SHEATH that enables >10% polarization on [1-C-13]-pyruvate, sufficient for successful in vivo experiments. By using SABRE catalyst-bound pyruvate at lower temperatures, approximately 20% polarization is accumulated and released into free pyruvate at elevated temperatures. A kinetic model of differential equations is developed to explain this effect and characterize critical relaxation and buildup parameters, demonstrating the combination of inexpensive hyperpolarization methods with low-cost MRI systems to achieve a broadly available, yet highly sensitive metabolic imaging platform.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Medicine, General & Internal
Lillian M. Lai, Andrea L. Gropman, Matthew T. Whitehead
Summary: Inborn errors of metabolism (IEM) are a group of disorders caused by functional defects in metabolic pathways. Despite being individually rare, they have a significant impact on the central nervous system of neonatal and infantile populations worldwide. Early diagnosis is crucial, but the nonspecific clinical manifestations and limitations of routine screening and genetic testing make neuroimaging a critical component in the evaluation process.
Article
Oncology
Noriaki Minami, Donghyun Hong, Nicholas Stevers, Carter J. Barger, Marina Radoul, Chibo Hong, Lee Chen, Yaewon Kim, Georgios Batsios, Anne Marie Gillespie, Russel O. Pieper, Joseph F. Costello, Pavithra Viswanath, Sabrina M. Ronen
Summary: This study indicates that MRS-detectable GSH, lactate, and lactate production could serve as metabolic biomarkers of response to emerging TERT-targeted therapies for GBM, ultimately improving patient management.
Article
Biochemistry & Molecular Biology
Norikazu Koyasu, Fuminori Hyodo, Ryota Iwasaki, Hinako Eto, Abdelazim Elsayed Elhelaly, Hiroyuki Tomita, Shinichi Shoda, Masaki Takasu, Takashi Mori, Masaharu Murata, Akira Hara, Yoshifumi Noda, Hiroki Kato, Masayuki Matsuo
Summary: In this study, the usefulness of spatiotemporal redox status as an early imaging biomarker of tumor response after irradiation using in vivo DNP-MRI was demonstrated. The results showed that in vivo DNP-MRI/CmP allowed faster and earlier visualization of tumor redox status responses compared to the verification of morphological changes observed with 1.5 T MRI and cancer metabolism obtained by hyperpolarized C-13 pyruvate MRS. The early assessment of redox status alterations with in vivo DNP-MRI/CmP probe may provide efficient information regarding the effectiveness of subsequent radiation treatment.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Henri de Maissin, Philipp R. Gross, Obaid Mohiuddin, Moritz Weigt, Luca Nagel, Marvin Herzog, Zirun Wang, Robert Willing, Wilfried Reichardt, Martin Pichotka, Lisa Hess, Thomas Reinheckel, Henning J. Jessen, Robert Zeiser, Michael Bock, Dominik von Elverfeldt, Maxim Zaitsev, Sergey Korchak, Stefan Gloeggler, Jan-Bernd Hoevener, Eduard Y. Chekmenev, Franz Schilling, Stephan Knecht, Andreas B. Schmidt
Summary: Metabolic magnetic resonance imaging (MRI) using hyperpolarized (HP) pyruvate is a non-invasive technique for diagnosing, staging, and monitoring response to treatment in various diseases. The Signal Amplification By Reversible Exchange (SABRE) method provides a fast and low-cost way to produce HP pyruvate. In this study, a novel routine to produce aqueous HP [1-C-13]pyruvate-d(3) for injection in 6 minutes was demonstrated, making HP pyruvate MRI available to a wide biomedical community for fast metabolic imaging of living organisms.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Tsung-Tai Lin, Cheng-Chuan Hu, Yi-Chih Hsu, Chih-Chien Wang, Shih-Wei Chiang, Chao-Ying Wang, Wei-Chou Chang, Guo-Shu Huang
Summary: Using MRS technology, we found that divers with hip pain had increased water content, increased unsaturation index of lipids, and decreased total lipid fraction in the femoral heads. These findings suggest that dysbaric changes in the femoral heads can be detected using MRS.
QUANTITATIVE IMAGING IN MEDICINE AND SURGERY
(2022)
Article
Chemistry, Multidisciplinary
Andrei A. Kuzhelev, Danhua Dai, Vasyl Denysenkov, Thomas F. Prisner
Summary: Dynamic nuclear polarization (DNP) is a powerful method to enhance NMR sensitivity. Recent research has found that similar to the solid effect and thermal mixing, DNP enhancements can be observed in the fluid phase. This discovery opens up new possibilities for DNP applications in macromolecular systems at ambient temperatures.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Oncology
Petra J. van Houdt, Yingli Yang, Uulke A. van der Heide
Summary: MRI-guided radiotherapy systems combine adaptive radiotherapy and biological targeting, updating treatment plans regularly to accommodate observed treatment responses. Quantitative imaging biomarkers are necessary for predicting treatment outcomes early in the process. This review provides an overview of the current evidence on the potential of quantitative MRI measurements as imaging biomarkers in MRI-guided radiotherapy systems.
FRONTIERS IN ONCOLOGY
(2021)
Review
Nanoscience & Nanotechnology
Philip Saul, Leif Schroder, Andreas B. B. Schmidt, Jan-Bernd Hovener
Summary: Nanomaterials are crucial in the development and application of hyperpolarized materials for MRI. They can function as both directly imaged hyperpolarized materials, carriers for hyperpolarized gases, and catalysts for generating hyperpolarized substrates. This article provides an overview of recent developments in these three application possibilities, highlighting carbon-based materials for direct imaging. The importance of nanomaterials in MRI is discussed, including their biocompatibility and imaging experiments.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Article
Medicine, Research & Experimental
Christian Prinz, Ludger Starke, Jason M. Millward, Ariane Fillmer, Paula Ramos Delgado, Helmar Waiczies, Andreas Pohlmann, Michael Rothe, Marc Nazare, Friedemann Paul, Thoralf Niendorf, Sonia Waiczies
Summary: This study successfully demonstrated the feasibility of using F-19 MR methods to detect TF in vivo, showing a significant correlation between TF concentrations and F-19 MR signals. The results highlight the potential application of F-19 MR technology in monitoring neuro-inflammation, emphasizing the need for further technological advancements in this field.
Review
Cardiac & Cardiovascular Systems
Salva R. Yurista, Robert A. Eder, Deborah H. Kwon, Christian T. Farrar, Yi Fen Yen, W. H. Wilson Tang, Christopher T. Nguyen
Summary: As one of the highest energy consuming organs in the body, the heart relies on various fuel sources to generate ATP for its continuous work. Magnetic resonance imaging (MRI) plays a crucial role in understanding the metabolic changes in the failing heart, and noninvasive quantification of metabolic changes has potential clinical applications. Novel MRI techniques, such as magnetic resonance spectroscopy (MRS), hyperpolarized MRS, and chemical exchange saturation transfer, provide detailed investigation of cardiac metabolism in heart failure.
EUROPEAN HEART JOURNAL-CARDIOVASCULAR IMAGING
(2022)
Letter
Radiology, Nuclear Medicine & Medical Imaging
Mariya S. Pravdivtseva, Philipp Berg, Jan-Bernd Hovener, Olav Jansen, Naomi Larsen
JOURNAL OF MAGNETIC RESONANCE IMAGING
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Rafat Chowdhury, Christoph A. Mueller, Lorna Smith, Fiona Gong, Marianthi-Vasiliki Papoutsaki, Harriet Rogers, Tom Syer, Saurabh Singh, Giorgio Brembilla, Adam Retter, Max Bullock, Lucy Caselton, Manju Mathew, Eoin Dineen, Thomas Parry, Juergen Hennig, Dominik von Elverfeldt, Andreas B. Schmidt, Jan-Bernd Hovener, Mark Emberton, David Atkinson, Alan Bainbridge, David G. Gadian, Shonit Punwani
Summary: This study aimed to validate a signal simulation framework for sequence parameter optimization and demonstrate the feasibility of using ME-bSSFP for HP C-13-MRI in patients. The results showed that ME-bSSFP allows for metabolic imaging of the prostate and can differentiate between aggressive prostate cancer and healthy tissue.
JOURNAL OF MAGNETIC RESONANCE IMAGING
(2023)
Article
Chemistry, Multidisciplinary
Christopher Nelson, Andreas B. Schmidt, Isaiah Adelabu, Shiraz Nantogma, Valerij G. Kiselev, Abubakar Abdurraheem, Henri de Maissin, Soeren Lehmkuhl, Stephan Appelt, Thomas Theis, Eduard Y. Chekmenev
Summary: The feasibility of Carbon-13 Radiofrequency (RF) Amplification by Stimulated Emission of Radiation (C-13 RASER) is demonstrated on a bolus of liquid hyperpolarized ethyl [1-C-13]acetate. The study successfully observed C-13 RASER signals and demonstrated its potential application on biomolecular carriers.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Andrey N. Pravdivtsev, Kai Buckenmaier, Nicolas Kempf, Gabriele Stevanato, Klaus Scheffler, Joern Engelmann, Markus Plaumann, Rainer Koerber, Jan-Bernd Hoevener, Thomas Theis
Summary: Nuclear spin hyperpolarization allows for real-time observation of metabolism and intermolecular interactions in vivo. The use of 1-13C-pyruvate as a hyperpolarized tracer is being evaluated in clinical trials and shows promise as a molecular imaging agent. However, the search for a simple, fast, and efficient hyperpolarization technique is ongoing.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Chemistry, Physical
Andreas B. Schmidt, James Eills, Laurynas Dagys, Martin Gierse, Michael Bock, Sebastian Lucas, Michael Bock, Ilai Schwartz, Maxim Zaitsev, Eduard Y. Chekmenev, Stephan Knecht
Summary: Efficient and rapid pyruvate hyperpolarization is achieved through signal amplification by reversible exchange (SABRE) with parahydrogen. This approach allows for high levels of polarization and paves the way for future in vivo translation of carbon-13 hyperpolarized molecular imaging probes.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Andreas B. Schmidt, Isaiah Adelabu, Christopher Nelson, Shiraz Nantogma, Valerij G. Kiselev, Maxim Zaitsev, Abubakar Abdurraheem, Henri de Maissin, Matthew S. Rosen, Soren Lehmkuhl, Stephan Appelt, Thomas Theis, Eduard Y. Chekmenev
Summary: Conventional nuclear magnetic resonance (NMR) is used to detect chemical transformations by exciting and detecting the precessing spins of nuclear spin ensembles. In this study, we employ Radiofrequency Amplification by Stimulated Emission of Radiation (RASER) to detect chemical transformations without the need for external radio frequency pulses or background signals. We investigated the hydrolysis of hyperpolarized ethyl-[1-C-13]-acetate using both conventional NMR and C-13 RASER and observed a complete disappearance and delayed reappearance of the RASER signal without destroying the C-13 polarization.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Nanoscience & Nanotechnology
Philip Saul, Leif Schroder, Andreas B. B. Schmidt, Jan-Bernd Hovener
Summary: Nanomaterials are crucial in the development and application of hyperpolarized materials for MRI. They can function as both directly imaged hyperpolarized materials, carriers for hyperpolarized gases, and catalysts for generating hyperpolarized substrates. This article provides an overview of recent developments in these three application possibilities, highlighting carbon-based materials for direct imaging. The importance of nanomaterials in MRI is discussed, including their biocompatibility and imaging experiments.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Debora Petroni, Costanza Fabbri, Serena Babboni, Luca Menichetti, Giuseppina Basta, Serena Del Turco
Summary: Extracellular vesicles (EVs) released by various cell types serve as mediators of intercellular signaling, conveying their cargo and acting as intermediaries for intracellular communication. In cardiovascular biology, EVs released by activated or apoptotic endothelial cells (EC-EVs) contribute to the development and progression of cardiovascular diseases. However, there is still limited knowledge about the role of intercommunication in healthy and vascular disease, as most data on EVs come from in vitro studies. Molecular imaging techniques play a crucial role in monitoring EVs biodistribution and communication networks in vivo.
Article
Biochemistry & Molecular Biology
Luise Schlotterose, Mariya S. Pravdivtseva, Frowin Ellermann, Olav Jansen, Jan-Bernd Hoevener, Frank D. Soennichsen, Francois Cossais, Ralph Lucius, Kirsten Hattermann
Summary: The role of microglia cells in neurodegenerative diseases has been increasingly recognized. Activation of microglia cells is believed to be involved in the progression of diseases like Alzheimer's or Parkinson's disease. In this study, the researchers investigated the effects of the natural antioxidant resveratrol on human microglia cells. They found that resveratrol reduced inflammation, increased the release of insulin-like growth factor 1, decreased glucose uptake, lowered mitochondrial activity, and attenuated cellular metabolism in microglia cells. The study focused on changes in metabolism without any external stressors and demonstrated the potential protective effects of resveratrol against neuroinflammation.
Article
Biochemistry & Molecular Biology
Carolin Kubelt, Dana Hellmold, Eva Peschke, Margarethe Hauck, Olga Will, Fabian Schuett, Ralph Lucius, Rainer Adelung, Regina Scherliess, Jan-Bernd Hoevener, Olav Jansen, Michael Synowitz, Janka Held-Feindt
Summary: Local drug delivery systems (LDDS) are potential therapies for glioblastoma (GBM), but their success has been limited. Establishing GBM animal models is crucial for evaluating LDDS, but few reports detail the process. In this study, a partial resection glioma model in rats was successfully established and used to evaluate the efficiency of LDDS. The results provide a powerful tool for future testing of these promising systems in clinical applications.
Article
Nanoscience & Nanotechnology
Brigida Silvestri, Paolo Armanetti, Giulio Pota, Giuseppe Vitiello, Alessandro Pezzella, Luca Menichetti, Vincenzo Giannini, Giuseppina Luciani
Summary: Photoacoustics is an increasingly recognized biomolecular imaging technique due to its non-invasiveness, deep penetration, high spatial resolution, and excellent endogenous contrast. Recent research has shown that a self-structured biocompatible nanoprobe, consisting of ternary hybrid nanoparticles with a silver core and a melanin component embedded into a silica matrix, exhibits excellent photoacoustic response. The interaction between eumelanin and silver is found to play an important role in the in situ-generated metal-organic interface. By systematically varying the metal/melanin component, the stability and intensity of the photoacoustic response can be tuned for various biomedical and theranostic applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Physical
Christian Bruns, Rainer Ringleb, Isabell Prediger, Frederike Euchner, Johannes Bernarding, Markus Plaumann
Summary: The interest in fluorinated substances has grown due to their diverse properties. NMR spectroscopy, an important analytical method, provides information on the structure and environment changes. Temperature plays a crucial role in most studies, however, measuring it is challenging in certain cases. Despite the difficulties, the potential applications in chemical reactors and medical fields are significant. Polyfluorinated molecules with separate 19F MR signals prove to be ideal for temperature determination without the need for a reference substance. The sensitivity of temperature is influenced by fluorine position and chemical environment.
Article
Chemistry, Physical
Felix Mysegaes, Peter Spiteller, Johannes Bernarding, Markus Plaumann
Summary: Magnetic resonance spectroscopy (MRS) and MR imaging (MRI) have become powerful non-invasive tools for medical diagnosis and therapy. F-19 MR shows promising potential due to the properties of fluorine atom and negligible background signals. This article reports the influence of solvent and structure on the temperature sensitivity of F-19 NMR signals and demonstrates the possibility of determining local temperature using chemical shift sensitivity.
Article
Chemistry, Multidisciplinary
Felix Mysegaes, Pauline Voigt, Peter Spiteller, Isabell Prediger, Johannes Bernarding, Markus Plaumann
Summary: F-19-based magnetic resonance is a powerful tool for overcoming difficulties in standard H-1 MR. We synthesized and characterized two Tm3+ complexes, including cell viability and stability tests. Both complexes allow temperature detection without a reference compound, with ΔC-T values of -0.2319 ppm K-1 and -0.2122 ppm K-1.
CHEMICAL COMMUNICATIONS
(2023)
Article
Gastroenterology & Hepatology
Rahul Unni, Nadia Andrea Andreani, Marie Vallier, Silke S. Heinzmann, Jan Taubenheim, Martina A. Guggeis, Florian Tran, Olga Vogler, Sven Kuenzel, Jan-Bernd Hoevener, Philip Rosenstiel, Christoph Kaleta, Astrid Dempfle, Daniel Unterweger, John F. Baines
Summary: Inflammatory bowel disease (IBD) is a chronic inflammatory condition that poses challenges for treatment. This study investigates the evolutionary changes of gut bacteria in IBD and finds disease-specific genetic and phenotypic changes associated with clinical relevance, including sensitivity to antibiotics.