Review
Environmental Sciences
Hanyu Fu, Chunyun Zhang, Yuanyuan Wang, Guofu Chen
Summary: As the eutrophication of natural water bodies worsens, harmful algal blooms (HABs) caused by harmful algae are becoming more frequent. HABs have become a global ecological problem that poses a serious threat to human health and food safety. It is crucial to establish methods for rapid detection of harmful algal species to provide early warning for HABs. Molecular biology techniques have provided new ideas for the detection of harmful algae and have become a research hotspot. However, current methods can only detect single harmful algal species at a time. Establishing methods for the simultaneous detection of multiple harmful algal species has become a new trend in molecular detection technology.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Chemistry, Analytical
Sung-Hun Yun, Ji-Sung Park, Seul-Bit-Na Koo, Chan-Young Park, Yu-Seop Kim, Jong-Dae Kim
Summary: This paper proposes a compact and cost-effective fluorescence detection system that can be integrated to a multiplex real-time PCR equipment. The system utilizes an open platform camera with embedded lens and a compact filter wheel, which performs comparably to photodiode systems in fluorescence detection.
Article
Microbiology
Yoshifumi Uwamino, Akio Aono, Yu Tomita, Kozo Morimoto, Masahiro Kawashima, Hirofumi Kamata, Yuka Sasaki, Hideaki Nagai, Naoki Hasegawa, Satoshi Mitarai
Summary: The Myco-Panel test accurately detects clinically common mycobacterial species that cause respiratory infections, providing useful information for patient management and rapid treatment initiation.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Qiuying Huang, Dongmei Chen, Chen Du, Qiaoqiao Liu, Su Lin, Lanlan Liang, Ye Xu, Yiqun Liao, Qingge Li
Summary: The article introduces a method called MeltArray, which allows for the detection of multiple targets in a single real-time PCR reaction. This method utilizes the enzyme activity of Taq DNA polymerase to generate fluorescent hybrids with different melting temperatures, enabling the detection of multiple targets. MeltArray has been validated with clinical samples and demonstrates the advantages of multiplicity, versatility, simplicity, and accessibility.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Food Science & Technology
Yuting Shang, Qinghua Ye, Qingping Wu, Rui Pang, Baoqing Zhou, Chufang Wang, Xinran Xiang, Fan Li, Juan Wang, Yinzhi Zhang, Jiasheng Wang, Xiulan Sun, Jumei Zhang
Summary: This study successfully identified species-specific molecular targets for different Cronobacter species using a bioinformatics approach and developed a multiplex PCR method for species identification. The PCR and mPCR assays showed excellent specificity and sensitivity for detecting Cronobacter species, suggesting their potential for use in food inspection and clinical diagnosis.
Article
Medical Laboratory Technology
Liu Dong, Weijia Li, Qianqian Xu, Jianfei Gu, Zhihua Kang, Jian Chen, Xiao Xu, Xinju Zhang, Xiuming Zhang, Haoqin Jiang, Ming Guan
Summary: The study aimed to establish a new multiplex dPCR detection system that can distinguish exact species of malaria by one PCR reaction. The results showed that the dPCR system could rapidly diagnose and quantify malaria DNA samples, with low analytical sensitivity and good sensitivity and specificity in clinical samples.
CLINICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Luca Miglietta, Ke Xu, Priya Chhaya, Louis Kreitmann, Kerri Hill-Cawthorne, Frances Bolt, Alison Holmes, Pantelis Georgiou, Jesus Rodriguez-Manzano
Summary: This study proposes a novel framework to identify and filter out nonspecific and low-efficient reactions from qdPCR data using outlier detection algorithms, aiming to improve the accuracy of machine learning methods. Additionally, an adaptive mapping filter strategy is developed to adjust the percentage of outliers removed based on the number of positive counts in qdPCR.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Juxin Yin, Liping Xia, Zheyu Zou, Jianjian Zhuang, Ying Mu
Summary: This study developed a digital PCR chip with multiplex capability and established a direct amplification detection method without nucleic acid extraction. The chip allows for accurate multiplex detection of ctDNA and eliminates the disadvantages of the extraction process.
Article
Engineering, Marine
Fangcao Zhao, Liang Guo, Nan Zhang, Kecheng Zhu, Jingwen Yang, Baosuo Liu, Huayang Guo, Dianchang Zhang
Summary: Cheilinus undulatus, a valuable seawater economic fish, is facing a rapid decline in population due to overfishing and habitat destruction. This study developed a multiplex PCR amplification system to assess the genetic diversity and inbreeding levels of C. undulatus specimens.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Food Science & Technology
M. A. Motalib Hossain, Siti Aimi Sarah Zainal Abidin, Aishah Bujang, Mohd Nasir Taib, Suresh Sagadevan, Mohd Rafie Johan, Nina Naquiah Ahmad Nizar
Summary: Meat and meat products are highly sought after globally for their high-quality protein and nutrients, but the industry faces challenges such as food fraud, mislabeling, and zoonotic diseases. Quantitative detection of the species origin using methods like PCR is crucial for food control. This review focuses on the design, development, and applications of TaqMan probe-based mqPCR techniques for authenticating meat products, providing a reference for academia and industry to improve existing assays.
Article
Chemistry, Analytical
Jixue Weng, Nan Sheng, Runyuan Wang, Shuo Liang, Chen Wang, Xue Bai, Guohua Zhou, Bingjie Zou, Qinxin Song
Summary: Cervical cancer is a significant cause of death in women, with HPV typing being crucial for diagnosis and control. The Multi-Vision method, utilizing closed-tube PCR, offers high specificity and sensitivity for typing various HPV types, making it a useful tool for HPV detection in resource-limited regions.
ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Zhendi Yu, Zhangkai Xu, Jiang Chen, Lili Chen, Ningbo Liao, Ronghua Zhang, Dongqing Cheng
Summary: Foodborne diseases are a critical food safety issue globally, with shellfish carrying various foodborne viruses posing a risk of infection. Factors such as age, season, and type of shellfish are associated with different levels of infection risk.
Article
Virology
Ryan V. Moriarty, Nicolas Fesser, Matthew S. Sutton, Vanessa Venturi, Miles P. Davenport, Timothy Schlub, Shelby L. O'Connor
Summary: This study modified a multiplex PCR and sequencing approach to characterize populations of simian immunodeficiency virus (SIV) isolated from nonhuman primates. The results showed that increasing starting template numbers improved reproducibility and decreased false positive identification, especially when using cDNA as the starting material. Ultimately, the study emphasizes the importance of rigorously validating methods to prevent overinterpretation of low frequency variants in a sample.
Article
Multidisciplinary Sciences
Li Sun, David G. Meckes
Summary: The new immuno-PCR method utilizes TotalSeq antibodies containing DNA conjugated oligos for high sensitivity and specificity profiling of multiple EV surface proteins simultaneously. This method has been successfully applied to cell and serum samples, and a micro-size exclusion chromatography method has been developed to detect EV surface proteins in as little as 10 μl of human serum when combined with immuno-PCR. Overall, this approach allows for rapid detection of multiple EV markers from small sample volumes in a single tube.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Baobao Lin, Zhi Geng, Yanjing Chen, Wu Zeng, Bao Li, Yan Zhang, Peng Liu
Summary: Adequate intake of folic acid is crucial during pregnancy, but certain genetic polymorphisms hinder its absorption, posing a threat to the lives of pregnant women and causing birth defects in newborns. Traditional laboratory testing for genetic variants related to folic acid metabolism is time-consuming and labor-intensive. However, a microfluidics-based molecular diagnostic system offers rapid, sensitive, high-throughput, and automated detection by integrating sample pre-processing and nucleic acid amplification on-chip.
Article
Biodiversity Conservation
C. Bozzuto, P. E. A. Hoeck, H. C. Bagheri, L. F. Keller
ANIMAL CONSERVATION
(2017)
Article
Evolutionary Biology
Christine Grossen, Iris Biebach, Samer Angelone-Alasaad, Lukas F. Keller, Daniel Croll
EVOLUTIONARY APPLICATIONS
(2018)
Article
Ecology
Benedikt Gehr, Elizabeth J. Hofer, Stefanie Muff, Andreas Ryser, Eric Vimercati, Kristina Vogt, Lukas F. Keller
Article
Biology
Pirmin Nietlisbach, Lukas F. Keller, Glauco Camenisch, Frederic Guillaume, Peter Arcese, Jane M. Reid, Erik Postma
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2017)
Article
Ecology
Benedikt Gehr, Elizabeth J. Hofer, Mirjam Pewsner, Andreas Ryser, Eric Vimercati, Kristina Vogt, Lukas F. Keller
ECOLOGY AND EVOLUTION
(2018)
Article
Behavioral Sciences
Benedikt Gehr, Elizabeth J. Hofer, Andreas Ryser, Eric Vimercati, Kristina Vogt, Lukas F. Keller
BEHAVIORAL ECOLOGY
(2018)
Article
Biodiversity Conservation
B. Nussberger, M. Currat, C. S. Quilodran, N. Ponta, L. F. Keller
BIOLOGICAL CONSERVATION
(2018)
Article
Biochemistry & Molecular Biology
D. M. Leigh, H. E. L. Lischer, C. Grossen, L. F. Keller
MOLECULAR ECOLOGY RESOURCES
(2018)
Article
Multidisciplinary Sciences
Kristof Veitschegger, Laura A. B. Wilson, Beatrice Nussberger, Glauco Camenisch, Lukas F. Keller, Stephen Wroe, Marcelo R. Sanchez-Villagra
SCIENTIFIC REPORTS
(2018)
Article
Ecology
Erica Ponzi, Lukas F. Keller, Timothee Bonnet, Stefanie Muff
Article
Ecology
Matthew E. Wolak, Peter Arcese, Lukas F. Keller, Pirmin Nietlisbach, Jane M. Reid
Article
Evolutionary Biology
Pirmin Nietlisbach, Stefanie Muff, Jane M. Reid, Michael C. Whitlock, Lukas F. Keller
EVOLUTIONARY APPLICATIONS
(2019)
Article
Ecology
Claudio Bozzuto, Iris Biebach, Stefanie Muff, Anthony R. Ives, Lukas F. Keller
NATURE ECOLOGY & EVOLUTION
(2019)
Article
Multidisciplinary Sciences
Christine Grossen, Frederic Guillaume, Lukas F. Keller, Daniel Croll
NATURE COMMUNICATIONS
(2020)
Article
Agriculture, Dairy & Animal Science
Stefanie Muff, Alina K. Niskanen, Dilan Saatoglu, Lukas F. Keller, Henrik Jensen
GENETICS SELECTION EVOLUTION
(2019)
