4.7 Article

Development of nine new microsatellite loci for the American beaver, Castor canadensis (Rodentia: Castoridae), and cross-species amplification in the European beaver, Castor fiber

Journal

MOLECULAR ECOLOGY RESOURCES
Volume 9, Issue 2, Pages 551-554

Publisher

WILEY
DOI: 10.1111/j.1755-0998.2008.02364.x

Keywords

beaver; Castor canadensis; Castor fibre; cross-species amplification success; microsatellite

Funding

  1. Tres Rios Wetlands, Phoenix Arizona
  2. Ministry of Education, Youth and Sport of the Czech Republic
  3. MSMT [0021620828]
  4. Ministry of Environment of the Czech Republic [VaV/620/1/03, SP/2d4/52/07]

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We developed nine new nuclear dinucleotide microsatellite loci for Castor canadensis. All loci were polymorphic, except for one. The number of alleles ranged from two to four and from five to 12 in populations from Arizona and Wisconsin, respectively. Average heterozygosity ranged from 0.13 to 0.86 per locus. Since cross-species amplification in Castor fiber was successful only in four loci, we tested also nine recently published C. canadensis loci in the Eurasian species. Eight of the published loci amplified; however, three were monomorphic. The number of alleles was lower in C. fiber than in C. canadensis at all loci tested.

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