Article
Biochemical Research Methods
Stephany D. Villota, Victoria E. Nipaz, Andres Carrazco-Montalvo, Sarah Hernandez, Jesse J. Waggoner, Patricio Ponce, Josefina Coloma, Alberto Orlando, Varsovia Cevallos
Summary: Alternative methods for rRT-PCR detection of SARS-CoV-2 without RNA extraction were investigated, and direct use of primary samples with an RNase inhibitor showed 100% sensitivity and specificity compared to standard protocols, which was validated in a second, independent laboratory.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Physiology
Jinrong Tang, Gemei Liang, Shaoqi Dong, Shuang Shan, Man Zhao, Xianru Guo
Summary: By evaluating the stability of reference genes in Athetis dissimilis under different conditions, suitable reference genes for different experimental conditions can be determined. Different reference genes are needed under different experimental conditions, providing a reliable basis for studying gene expression in A. dissimilis.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Plant Sciences
Fangzhou Zhao, Nathan A. Maren, Pawel Z. Kosentka, Ying-Yu Liao, Hongyan Lu, James R. Duduit, Debao Huang, Hamid Ashrafi, Tuanjie Zhao, Alejandra Huerta, Thomas G. Ranney, Wusheng Liu
Summary: Optimizing primer design and qPCR parameters are crucial for improving primer efficiency and data analysis accuracy.
HORTICULTURE RESEARCH
(2021)
Article
Microbiology
Shu Li, Ping He, Huacai Fan, Lina Liu, Kesuo Yin, Baoming Yang, Yongping Li, Su-Mei Huang, Xundong Li, Si-Jun Zheng
Summary: This study identified key genes showing strong antibiotic activity against Fusarium wilt of banana in five Bacillus strains, providing insights into different mechanisms involved in combating the TR4 pathogen.
Article
Virology
Jessika Cristina Chagas Lesbon, Mirele Daiana Poleti, Elisangela Chicaroni de Mattos Oliveira, Jose Salvatore Leister Patane, Luan Gaspar Clemente, Vincent Louis Viala, Gabriela Ribeiro, Marta Giovanetti, Luiz Carlos Junior de Alcantara, Olivia Teixeira, Maria Cristina Nonato, Loyze Paola Oliveira de Lima, Antonio Jorge Martins, Claudia Renata dos Santos Barros, Elaine Cristina Marqueze, Jardelina de Souza Todao Bernardino, Debora Botequio Moretti, Ricardo Augusto Brassaloti, Raquel de Lello Rocha Campos Cassano, Pilar Drummond Sampaio Correa Mariani, Svetoslav Nanev Slavov, Rafael Bezerra dos Santos, Evandra Strazza Rodrigues, Elaine Vieira Santos, Josiane Serrano Borges, Debora Glenda Lima de La Roque, Joao Paulo Kitajima, Bibiana Santos, Patricia Akemi Assato, Felipe Allan da Silva da Costa, Cecilia Artico Banho, Livia Sacchetto, Marilia Mazzi Moraes, Melissa Palmieri, Fabiana Erica Vilanova da Silva, Rejane Maria Tommasini Grotto, Jayme A. Souza-Neto, Mauricio Lacerda Nogueira, Luiz Lehman Coutinho, Rodrigo Tocantins Calado, Raul Machado Neto, Dimas Tadeu Covas, Simone Kashima, Maria Carolina Elias, Sandra Coccuzzo Sampaio, Heidge Fukumasu
Summary: The article highlights the evolution of the COVID-19 virus and the potential impact on RT-PCR testing accuracy, identifying gene mutations that affect N gene detection and emphasizing the importance of continuous monitoring of genetic mutations to ensure diagnostic effectiveness.
Article
Pathology
Reena Yadav, Laxmi Kumari, Sumit Goel, Deepinder Kaur, Divya Dahiya, Yashwant Kumar, Pranab Dey, Alka Bhatia
Summary: Touch imprints can improve the quality and concentration of RNA extracted from breast tissue, providing a fast and cost-effective strategy to ensure that the extracted RNA is representative of the tumor.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Infectious Diseases
Georgi Tomov, Nikola Stamenov, Deyan Neychev, Kiril Atliev
Summary: This study aimed to examine the possible association between tongue piercing and Candida spp. infection in healthy individuals. The results suggest that the main risk factors for tongue colonization with Candida spp. are gender, smoking, and improper hygiene, while tongue piercing is not a risk factor for colonization of Candida spp.
Article
Medicine, General & Internal
Sayamon Hongjaisee, Yosita Jabjainai, Suthasinee Sakset, Kanya Preechasuth, Nicole Ngo-Giang-Huong, Woottichai Khamduang
Summary: This study evaluated the efficacy of four simple RNA extraction methods for HCV detection and found that the silica-membrane-based and magnetic-beads-based methods had higher detection rates for RNA extraction from plasma. Magnetic-beads-based extraction can be used as an alternative method for on-site HCV detection.
Article
Virology
Yolanda I. I. Ho, Ann H. Wong, Eddie C. M. Leung, River C. W. Wong, Raymond W. M. Lai
Summary: By adapting and evaluating testing methods, as well as continuously training staff, high-quality screening services for SARS-CoV-2 can be provided within time constraints.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kathannan Sankar, Hyung Joo Yoon, Young Bo Lee, Kyeong Yong Lee
Summary: Bumble bees are important alternative pollinators and model insects. This study identified stable reference genes for the bumble bee species and their different lines and tissues using RT-qPCR procedures and four analysis programs. The results emphasized the need to evaluate the stability of candidate reference genes for any differently designed lines and tissue conditions in bumble bee species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physiology
Guo Ding, Qionghua Gao, Jun Chen, Jie Zhao, Guojie Zhang, Weiwei Liu
Summary: This study validates reliable reference genes for RT-qPCR analysis in pharaoh ants and lays the foundation for future research.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Veterinary Sciences
Siti Tasnim Makhtar, Sheau Wei Tan, Nur Amalina Nasruddin, Nor Azlina Abdul Aziz, Abdul Rahman Omar, Farina Mustaffa-Kamal
Summary: A specific TaqMan-based real-time RT-PCR assay targeting the N gene of FeMV was developed in this study, showing higher detection rate and sensitivity in early detection of FeMV infection.
BMC VETERINARY RESEARCH
(2021)
Article
Multidisciplinary Sciences
Huseyin Tombuloglu, Hussein Sabit, Ebtesam Al-Suhaimi, Reem Al Jindan, Khaled R. Alkharsah
Summary: The study developed a multiplex rRT-PCR diagnostic method targeting two viral genes and one human gene, which was validated to be highly consistent with the CDC authorized diagnostic system, providing an accurate diagnostic approach for SARS-CoV-2.
Article
Immunology
Danna Zhou, Shuangshuang Wang, Keli Yang, Xue Liu, Wei Liu, Rui Guo, Wan Liang, Fangyan Yuan, Zewen Liu, Ting Gao, Yong-xiang Tian
Summary: A new real-time nucleic acid sequence-based amplification (RT-NASBA) method was developed for rapid detection of Japanese encephalitis virus (JEV), showing high specificity and sensitivity.
MICROBIAL PATHOGENESIS
(2021)