Article
Biochemistry & Molecular Biology
Bi Zhao, Akila Katuwawala, Christopher J. Oldfield, Gang Hu, Zhonghua Wu, Vladimir N. Uversky, Lukasz Kurgan
Summary: This study focused on the interaction between RNA-binding proteins (RBPs) and specific RNA types, revealing varying levels of intrinsic disorder in different RNA types. The results showed that disorder is relatively rarely utilized in RNA-binding regions, while disorder in non-RNA-binding regions may be involved in posttranslational modifications and interactions with DNA.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Ophthalmology
David J. Taylor Gonzalez, Mak Djulbegovic, Michael Antonietti, Matthew Cordova, Guy W. Dayhoff II, Robby Mattes, Anat Galor, Vladimir N. Uversky, Carol L. Karp
Summary: The purpose of this study was to characterize the proteome of human tears and investigate the presence of intrinsically disordered proteins (IDPs). The findings revealed an abundance of IDPs in tears, indicating their significant role in protein function and interaction networks. These findings have promising implications for disease biomarker identification and drug target development.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Review
Biology
Elrashdy M. Redwan, Abdullah A. Aljadawi, Vladimir N. Uversky
Summary: This study examines the interplay between protein intrinsic disorder, hepatitis C virus (HCV) infection, and signaling pathways induced by Toll-like receptors (TLRs). The results demonstrate that proteins involved in the regulation and execution of innate immunity pathways and in HCV-TLR interaction have substantial levels of intrinsic disorder, indicating the important role of intrinsic disorder in virus-host warfare.
Article
Biochemistry & Molecular Biology
Goran Vinterhalter, Jovana J. Kovacevic, Vladimir N. Uversky, Gordana M. Pavlovic-Lazetic
Summary: The research investigates the correlation between protein structure and function using different datasets and functional annotations. Results show consistent findings in protein disorder prediction between Gene Ontology (GO terms) and Swiss-Prot keywords. Additionally, a new method for deriving missing mappings between GO terms and Swiss-Prot keywords is proposed based on protein function similarity measurement.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biology
Aaron S. Mohammed, Vladimir N. Uversky
Summary: Proteomic analysis of the Heslington brain, a 2600-year-old brain tissue, revealed the preservation of many proteins. These proteins, along with others found in the brain, have a high level of intrinsic disorder, which may play a crucial role in preserving the brain tissue.
Article
Genetics & Heredity
Bingxin Zhao, Tengfei Li, Stephen M. Smith, Di Xiong, Xifeng Wang, Yue Yang, Tianyou Luo, Ziliang Zhu, Yue Shan, Nana Matoba, Quan Sun, Yuchen Yang, Mads E. Hauberg, Jaroslav Bendl, John F. Fullard, Panagiotis Roussos, Weili Lin, Yun Li, Jason L. Stein, Hongtu Zhu
Summary: Association analyses using resting-state functional magnetic resonance images have identified common genetic variants influencing intrinsic brain activity. These variants are correlated with neuropsychiatric traits and disorders, providing insights into the genetic architecture of brain functional networks and their links to brain-related complex traits and disorders.
Article
Chemistry, Physical
Shahar Zuri, Arthur Shapiro, Leeor Kronik, Efrat Lifshitz
Summary: In this work, the anharmonic ground state of the (PEA)(2)PbI4 compound was thoroughly investigated using low-temperature X-ray diffraction and photoluminescence spectroscopy, supported by density functional theory calculations. It was found that the ground state has two coexisting chiral sublattices and uneven anharmonicity, where the state population can be tuned by surface effects. These findings uncover a disordered ground state that may induce intrinsic grain boundaries, which are important in practical applications.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Virology
Guillaume Lafforgue, Thierry Michon, Justine Charon
Summary: Intrinsically disordered regions (IDRs) in the proteome of RNA viruses exhibit higher mutational robustness than highly structured regions (ORs). Substitutions in the ORs are constrained by physico-chemical properties, while substitutions in IDRs tend to be more random. IDRs may serve as a potential adaptive reservoir.
Review
Biochemical Research Methods
Vladimir N. Uversky, Abdulgader H. Albar, Rizwan H. Khan, Elrashdy M. Redwan
Summary: Royal Jelly is a gelatinous fluid secreted by nurse honeybees, containing nutritive components like carbohydrates, proteins, lipids, vitamins, and mineral salts. It also has cosmetic and health-promoting properties, displaying multifunctional biological activities likely due to the structural heterogeneity and polymorphism of the proteins and peptides it contains.
Article
Biochemical Research Methods
Lukasz Kurgan
Summary: This review provides an overview of the development and practical resources in the field of intrinsic disorder prediction. These resources include predictors, meta webservers, databases, and quality assessment tools, which will facilitate the application of disorder predictions in various fields such as rational drug design, systems medicine, and structural genomics.
Article
Biochemistry & Molecular Biology
H. Jane Dyson, Peter E. Wright
Summary: Nuclear magnetic resonance (NMR) has been crucial in the study of intrinsically disordered proteins (IDPs) and regions (IDRs), providing valuable insights when combined with other biophysical methods. Recent advancements have been made in studying proteins with both ordered and disordered domains, as well as problematic sequences with repeated amino acid tracts. Innovative applications of NMR have offered new insights into protein aggregation mechanisms and interactions of disordered proteins with their targets. NMR remains invaluable in understanding the structural ensembles, dynamic behavior, and functional mechanisms of IDPs and IDRs.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Shuqi Luo, Samuel Wohl, Wenwei Zheng, Sichun Yang
Summary: Protein intrinsic disorder is recognized for its biological and disease-driven functions, but its high conformational flexibility presents challenges for biophysical studies. Complementary experimental and computational methods, as well as integrative strategies, provide valuable insights into the sequence-structure-function relationship of disordered proteins. Recent advances in small molecule inhibitors targeting disordered N-terminal domains of vital transcription factors are summarized.
Review
Biochemistry & Molecular Biology
Manon Julien, Rania Ghouil, Ambre Petitalot, Sandrine M. Caputo, Aura Carreira, Sophie Zinn-Justin
Summary: BRCA2 protein plays essential roles in DNA repair, replication protection, and cell division. Pathogenic variants in BRCA2 can lead to genome instability and are associated with breast and/or ovarian cancers. The disordered regions in BRCA2 are predicted to mediate important interactions during DNA repair, mitosis, and meiosis.
Article
Biochemistry & Molecular Biology
Guy W. W. Dayhoff, Vladimir N. N. Uversky
Summary: This article presents RIDAO, a high-efficiency web-based disorder predictor for protein intrinsic disorder analysis. RIDAO integrates six disorder predictors into a unified platform, offering high accuracy and processing efficiency.
Article
Biochemistry & Molecular Biology
Bi Zhao, Akila Katuwawala, Vladimir N. Uversky, Lukasz Kurgan
Summary: The study found that human proteins are significantly enriched in intrinsic disorder, with proteins localized in the nucleus and cytoskeleton having higher levels of disorder. On average, human proteins are assigned to 2.3 subcellular compartments, with proteins localized to fewer compartments being more disordered. Disordered proteins in the most disorder-enriched subcellular compartments primarily interact with nucleic acids and protein partners, adding a missing piece to the puzzle of functional disorder within the cell.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
