Article
Multidisciplinary Sciences
Sachiko Fujiwara, Thanh Phuong Nguyen, Kyoko Furuse, Yugo Fukazawa, Tetsuhisa Otani, Mikio Furuse
Summary: The interaction of claudins with ZO proteins is not a prerequisite for tight junction formation at the most apical part of cell-cell contacts.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Boglarka Zambo, Gergo Gogl, Bastien Morlet, Pascal Eberling, Luc Negroni, Herve Moine, Gilles Trave
Summary: This study comprehensively investigates the PDZ-PBM interactome of diacylglycerol kinases (DGKs) using interactomic approaches and evolutionary analysis, revealing functional differences between PBMs of different types of DGKs.
Article
Biochemistry & Molecular Biology
Wareerat Umnajvijit, Jariya Sangthong, Fabien Loison, Kulthida Vaeteewoottacharn, Mathurose Ponglikitmongkol
Summary: This study identifies the internal class III PBM of HPV16E6*I as a key element required for Dlg degradation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Physiology
Jianchun Chen, Xiaoyong Wang, Qian He, Raymond C. Harris
Summary: Podocyte is a crucial component of the glomerular filtration barrier, and the role of Yes-associated protein (YAP)/transcriptional coactivator with PDZ-binding motif (TAZ) in podocytes has been investigated. The study found that TAZ is constitutively active in podocytes and plays a critical role in the expression of synaptopodin, zonula occludens-1, and zonula occludens-2, which are important for podocyte function and structure.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Emilie Giraud, Chloe Otero del Val, Celia Caillet-Saguy, Nada Zehrouni, Cecile Khou, Joel Caillet, Yves Jacob, Nathalie Pardigon, Nicolas Wolff
Summary: The study revealed that the PBM of NS5 from West Nile virus interacts with PDZ-containing proteins in human cells, playing a crucial role in viral replication. Knockdown of specific PDZ-containing proteins resulted in decreased WNV replication, highlighting the significance of these interactions in the virus life cycle.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Mariano Martin, Lisa Salleron, Victoria Peyret, Romina Celeste Geysels, Elisabeth Darrouzet, Sabine Lindenthal, Carlos Eduardo Bernal Barquero, Ana Maria Masini-Repiso, Thierry Pourcher, Juan Pablo Nicola
Summary: NIS gene mutations leading to impaired iodide accumulation cause congenital hypothyroidism, as demonstrated by a truncated NIS protein missing some amino acids mislocalized into intracellular vesicles. Further investigation reveals the involvement of the PDZ domain in the interaction between the protein SCRIB and NIS, affecting the plasma membrane localization of NIS.
Article
Biochemistry & Molecular Biology
Celia Caillet-Saguy, Fabien Durbesson, Veronica V. Rezelj, Gergo Gogl, Quang Dinh Tran, Jean-Claude Twizere, Marco Vignuzzi, Renaud Vincentelli, Nicolas Wolff
Summary: Small linear motifs targeting the PDZ domains have been identified at the C terminus of SARS-CoV-2 proteins E, 3a, and N. By profiling the human PDZome, potential human protein targets of SARS-CoV-2 have been identified, some of which may play roles in viral replication and immune responses.
Article
Cell Biology
Soheil Ghassemi, Tara Asgari, Hadi Mirzapour-Delavar, Shayan Aliakbari, Hamid Gholami Pourbadie, Christophe Prehaud, Monique Lafon, Alireza Gholami, Kayhan Azadmanesh, Nima Naderi, Mohammad Sayyah
Summary: The study demonstrates that the rabies virus glycoprotein (RVG) affects synaptic function in the hippocampal dentate gyrus of rats, facilitating both short- and long-term synaptic plasticity by potentially involving both pre- and postsynaptic mechanisms to alter synaptic function. Further research is needed to elucidate the underlying mechanisms.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hai-Fan Yu, Lian-Wen Zheng, Zhan-Qing Yang, Yu-Si Wang, Ting-Ting Wang, Zhan-Peng Yue, Bin Guo
Summary: The protein TAZ plays a crucial role in preparing the uterus for pregnancy by promoting stromal cell proliferation and protecting against oxidative damage. Its involvement in enhancing cellular antioxidant capacity and moderating mitochondrial function highlights its significance in maintaining the integrity of stromal cell differentiation during uterine decidualization.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Sunayana Dagar, Kumari Pushpa, Diksha Pathak, Sarbani Samaddar, Anjana Saxena, Sourav Banerjee, Sivaram V. S. Mylavarapu
Summary: This study demonstrates a novel mRNA-guided mechanism of TNT formation through the maintenance of cellular 14-3-3 zeta mRNA levels by the RBP nucleolin.
Article
Cell Biology
Michelle America, Naguissa Bostaille, Marie Eubelen, Maud Martin, Didier Y. R. Stainier, Benoit Vanhollebeke
Summary: This study reveals the molecular mechanisms underlying the interactions between Gpr124 and Frizzled in zebrafish and mammals, and provides insights into the evolution of Gpr124/Reck function in vertebrates.
Article
Biochemistry & Molecular Biology
Mariano Genera, Baptiste Colcombet-Cazenave, Anastasia Croitoru, Bertrand Raynal, Ariel Mechaly, Joel Caillet, Ahmed Haouz, Nicolas Wolff, Celia Caillet-Saguy
Summary: The protein tyrosine phosphatase non-receptor type 3 (PTPN3) has a PDZ domain that plays both tumor-suppressive and tumor-promoting roles in cancer. This study focuses on the interactions between the PDZ domain of PTPN3 and the PDZ-binding motifs (PBMs) of viral and cellular protein partners. The study identifies key structural determinants of PBM recognition by PTPN3 and reveals the inhibitory role of the PDZ domain on the protein's phosphatase activity.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Microbiology
Yanlei Zhu, Flavio Alvarez, Nicolas Wolff, Ariel Mechaly, Sebastien Brule, Benoit Neitthoffer, Sandrine Etienne-Manneville, Ahmed Haouz, Batiste Boeda, Celia Caillet-Saguy
Summary: The C-terminus of the SARS-CoV-2 protein E contains a PDZ-binding motif (PBM) that interacts with human PDZ-containing proteins involved in cellular junctions and polarity. Crystal structures of complexes between human LNX2, MLLT4, and MPP5 PDZs and SARS-CoV-2 E PBM were solved, revealing the binding preferences of the PBM for cellular targets. Mutations in the E protein near the PBM may have important effects on its structure and ion-channel activity, as well as the host machinery targeted by the variants during infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jihyun Lee, Yujin Jung, Seo won Jeong, Ga Hee Jeong, Gue Tae Moon, Miri Kim
Summary: The Hippo signaling pathway and its effectors, YAP and TAZ, are found to play critical roles in angiogenesis and have potential therapeutic implications for rosacea. In a mouse model, inhibiting YAP and TAZ improved clinical features of rosacea and reduced VEGF immunoreactivity, suggesting their potential as therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Koya Miura, Youichi Suzuki, Kotaro Ishida, Masashi Arakawa, Hong Wu, Yoshihiko Fujioka, Akino Emi, Koki Maeda, Ryusei Hamajima, Takashi Nakano, Takeshi Tenno, Hidekazu Hiroaki, Eiji Morita
Summary: This study established a system for producing SARS-CoV-2 virus-like particles (VLPs) and confirmed the important role of the E protein in VLP release. Mutations in the amyloidization motif and the PDZ-binding motif of the E protein affected its function in VLP release.
JOURNAL OF VIROLOGY
(2023)