Article
Food Science & Technology
Hongxuan Li, Shiqian Fu, Danliangmin Song, Xue Qin, Wei Zhang, Chaoxin Man, Xinyan Yang, Yujun Jiang
Summary: In this study, 35 Cronobacter strains isolated from powdered infant formula and its processing environment were identified and typed using 16S rRNA sequencing and multilocus sequence typing (MLST) technology. A total of 35 different sequence types were obtained, including three new ones that were isolated for the first time. The antibiotic resistance analysis revealed that all isolates were resistant to erythromycin but sensitive to ciprofloxacin. Moreover, 68.57% of the strains showed multi-drug resistance, with the most drug-resistant Cronobacter strain exhibiting resistance to 13 different drugs. Transcriptomics analysis identified 77 differentially expressed genes related to drug resistance, and the study further revealed that Cronobacter strains can activate multidrug efflux systems to enhance drug resistance. Understanding the drug resistance and mechanisms of Cronobacter is of great public health significance for the rational selection and development of antibacterial drugs, as well as the control and treatment of infections caused by this pathogenic microorganism.
Article
Food Science & Technology
Hua Jiang, Yue Xiang, Xiaojuan He, Chencheng Li, Fuxing Lin, Jihong Shao, Yuanhong Li
Summary: This study investigated the prevalence and characterization of Cronobacter spp. in dried edible mushrooms in Jiangsu province, China. The overall prevalence of Cronobacter spp. in dried edible mushrooms was 14.8%, with high genetic diversity observed. Some Cronobacter strains showed resistance to certain antibiotics, although they were susceptible to most tested antibiotics. Most isolates showed weak biofilm formation ability. This study provides new epidemiological evidence for the presence of Cronobacter spp. in dried edible mushrooms.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Immunology
Pao-Yu Chen, Yu-Chung Chuang, Jann-Tay Wang, Wang-Huei Sheng, Yee-Chun Chen, Shan-Chwen Chang
Summary: The study revealed the increasing prevalence of ST8/USA300 MRSA bacteremia in Asia, primarily in community settings. Patients with ST8/USA300 bacteremia had higher rates of diabetes, were more likely to come from long-term care facilities, and often had skin and soft tissue infections as their primary focus. Long-term surveillance is recommended to monitor the dissemination and pathogenicity of ST8/USA300 MRSA.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Biochemistry & Molecular Biology
Stefano Ravaioli, Davide Campoccia, Werner Ruppitsch, Franz Allerberger, Alessandro Poggi, Emanuele Chisari, Lucio Montanaro, Carla Renata Arciola
Summary: This study evaluated the usefulness of automated ribotyping as a screening tool for identifying S. aureus genetic lineages. Results showed that automated ribotyping can predict spa and MLST clonal complexes, but its high cost and lower discriminatory power could be obstacles for fine genotyping analysis. However, automated ribotyping has advantages such as automation, ease of execution, stability, typeability, and reproducibility, making it a reliable method to be used alongside gold standard methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Pamela Barbadoro, Daniela Bencardino, Elisa Carloni, Enrica Omiccioli, Elisa Ponzio, Rebecca Micheletti, Giorgia Acquaviva, Aurora Luciani, Annamaria Masucci, Antonella Pocognoli, Francesca Orecchioni, Marcello Mario D'Errico, Mauro Magnani, Francesca Andreoni
Summary: This study found a high prevalence of CPE in patients upon their admission, with over 50% of the strains being multidrug-resistant. The most prevalent plasmid profile was FIIK, FIB KQ (33%) and the most common sequence type was ST512.
Article
Infectious Diseases
Cindy Shuan Ju Teh, Polly Soo Xi Yap, Noorfatin Jihan Zulkefli, Prasanna Subramaniam, Pik San Sit, Zhi Xian Kong, Chun Wie Chong, Kumutha Malar Vellasamy, Vanitha Mariappan, Sasheela Ponnampalavanar, Jamuna Vadivelu
Summary: The study conducted MLST analysis on B. pseudomallei strains in Malaysia, revealing a high diversity of sequence types and indicating localized evolution giving rise to new STs, with host and environmental factors playing a crucial role in the evolutionary changes of B. pseudomallei.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Agriculture, Dairy & Animal Science
Chuncai Liang, Huan Cui, Ligong Chen, Hailong Zhang, Cheng Zhang, Juxiang Liu
Summary: This study investigated the antimicrobial resistance and prevalence of Escherichia coli (E. coli) in broiler chickens in Hebei Province. The results showed that ampicillin resistance was the highest, while meropenem resistance was the lowest. There was a serious problem of multidrug resistance in both types of broiler farms.
Article
Agriculture, Dairy & Animal Science
Xinyuan Zhao, Wei Li, Shaopeng Hou, Yanjun Wang, Shuyang Wang, Jing Gao, Ruihua Zhang, Shijin Jiang, Yanli Zhu
Summary: In this study, an epidemiological investigation was conducted on drug resistance of 110 Salmonella strains isolated from multiple duck farms in Shandong Province and surrounding areas, China. The study found high rates of multidrug resistance and detected multiple drug resistance genes. The results also revealed the important role of class I integrons and plasmids in the transmission of drug resistance genes.
Article
Virology
Jie Jiang, Guanda Lan, Jinghua Li, Jun Yu, Honglan Huang, Yanbo Sun, Cuiting Xu, Dandan Liu, Yunwei Gong, Chunyan Zhao
Summary: The lytic bacteriophage JC01, which infects C. sakazakii, was successfully isolated and its complete genome sequence was determined. The genome of JC01 is composed of double-stranded DNA with a length of 61,736 bp and a G + C content of 58.9%. It contains 76 putative open reading frames (ORFs) and belongs to a novel phage infecting C. sakazakii.
ARCHIVES OF VIROLOGY
(2023)
Article
Microbiology
Alicia C. Bertolotti, Eva Forsgren, Marc O. Schaefer, Fabrice Sircoulomb, Nicolas Gaiani, Magali Ribiere-Chabert, Laurianne Paris, Pierrick Lucas, Claire de Boisseson, Joakim Skarin, Marie-Pierre Riviere
Summary: This study introduces a standardized and robust cgMLST scheme for typing P. larvae, which allows for high-resolution differentiation and successful outbreak tracking. Compared to traditional MLST methods, cgMLST can provide finer differentiation of samples and maintains high stability and standardization for sharing results and methods across different laboratories.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Cell Biology
Benshak J. Audu, Strachan Norval, Lopes Bruno, Ramjee Meenakshi, Macrae Marion, Ken J. Forbes
Summary: This study in Nigeria investigated the genomic diversity and antimicrobial resistance profile of Campylobacter in humans and livestock, finding a higher prevalence of resistance in Campylobacter isolated from livestock compared to humans. The results contribute to the understanding of the epidemiology of thermophilic Campylobacter in Nigeria.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Microbiology
Mei Yuan, Mingbiao Ma, Hongchao Jiang, Mao Fan, Ying Sun, Bailing Zhou, Xingxing Feng, Junyi Yang, Min Su, Xiaoli He
Summary: The aim of this study was to determine the distribution of capsular serotypes and antimicrobial drug resistance patterns of Haemophilus influenzae in the Kunming region of China. The results showed that the majority of the isolates were non-typeable H. influenzae, with high prevalence of beta-lactamase-producing strains that were resistant to multiple antibiotics.
POLISH JOURNAL OF MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Susana Blanco, Cristina Sanz, Maria P. Gutierrez, Maria Simarro, Irene Lopez, Isabel Escribano, Jose M. Eiros, Pilar Zarzosa, Antonio Orduna, Juan C. Lopez, Gabriel A. March
Summary: The study evaluated the effectiveness of using MALDI-TOF technology for Legionella pneumophila typing, confirming the ability to quickly and accurately identify different STs, and to obtain ST information directly from bacterial colonies grown on BCYE agar.
JOURNAL OF MICROBIOLOGICAL METHODS
(2021)
Article
Infectious Diseases
Aghata Cardoso da Silva Ribeiro, Fernanda Fernandes Santos, Ikechukwu Benjamin Moses, Luciene Andrade da Rocha Minarini, Ana Cristina Gales
Summary: In this study, a new fast, economic, and effective typing technique for carbapenem-resistant Klebsiella pneumoniae (CR-KPN) was explored by analyzing the fosA(KP) sequence. The results showed that chromosomal fosA(KP) sequences are highly conserved in different STs and CCs, providing a useful tool to identify the epidemiological STs for KPN and discriminate them from CC258/11.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Microbiology
Kiyoharu Fukushima, Yuki Matsumoto, Takanori Matsuki, Haruko Saito, Daisuke Motooka, Sho Komukai, Eriko Fukui, June Yamuchi, Tadayoshi Nitta, Takayuki Niitsu, Yuko Abe, Hiroshi Nabeshima, Yasuharu Nagahama, Takuro Nii, Kazuyuki Tsujino, Keisuke Miki, Seigo Kitada, Atsushi Kumanogoh, Shizuo Akira, Shota Nakamura, Hiroshi Kida
Summary: To determine the treatment strategy for nontuberculous mycobacterial pulmonary disease, a simplified workflow involving direct sequencing of mycobacterial cultures was developed. The direct MGIT-seq method achieved accurate identification of species and subspecies as well as detection of drug resistance, making it a valuable tool for clinical practice.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
K. Yarnell, M. Le Bon, N. Turton, M. Savova, A. McGlennon, S. Forsythe
JOURNAL OF APPLIED MICROBIOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Marcelo Luiz Lima Brandao, Natalia Scudeller Umeda, Emily Jackson, Stephen James Forsythe, Ivano de Filippis
Article
Ecology
Julia M. Maritz, Krysta H. Rogers, Tara M. Rock, Nicole Liu, Susan Joseph, Kirkwood M. Land, Jane M. Carlton
Article
Immunology
Ondrej Holy, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Igor Hochel, Julio Parra-Flores, Jana Petrzelova, Kamila Facevicova, Stephen Forsythe, Abdlrhman Alsonosi
MICROBIAL PATHOGENESIS
(2019)
Article
Dentistry, Oral Surgery & Medicine
M. A. Payne, A. Hashim, A. Alsam, S. Joseph, J. Aduse-Opoku, W. Wade, M. A. Curtis
JOURNAL OF DENTAL RESEARCH
(2019)
Article
Microbiology
Susan M. Joseph, Thomas Battaglia, Julia M. Maritz, Jane M. Carlton, Martin J. Blaser
Article
Immunology
L. Monin, D. S. Ushakov, H. Arnesen, N. Bah, A. Jandke, M. Munoz-Ruiz, J. Carvalho, S. Joseph, B. C. Almeida, M. J. Green, E. Nye, S. Hatano, Y. Yoshikai, M. Curtis, H. Carlsen, U. Steinhoff, P. Boysen, A. Hayday
MUCOSAL IMMUNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
P. V. Costa, R. M. de Siqueira, A. C. Rosa Guimaraes, L. Vasconcellos, V. Midlej, G. M. Silva da Conceicao, S. J. Forsythe, M. L. Lima Brandao
Summary: This study evaluated the cytotoxic activity of Cronobacter strains isolated from foods and clinical samples in Brazil, and identified some strains capable of producing cytotoxins.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Food Science & Technology
Na Ling, Yujun Jiang, Haiyan Zeng, Yu Ding, Stephen Forsythe
Summary: This review discusses the progress made in China regarding the research on Cronobacter, including large-scale surveys and the adoption of DNA sequence-based methods for identification and genotyping of the organism. These methods have significantly advanced understanding of the taxonomy, ecology, and virulence of Cronobacter, leading to improved source tracking in infant formula production facilities and epidemiological investigations.
JOURNAL OF FOOD SCIENCE
(2021)
Review
Dentistry, Oral Surgery & Medicine
Susan Joseph, Michael A. Curtis
Summary: Recent advances in research on microbial populations in the human mouth provide new insights into the mechanisms underlying periodontal health and disease. The resilience of healthy and diseased microbial communities may be influenced by the genetics of the host and the makeup of the acquired microbiome.
PERIODONTOLOGY 2000
(2021)
Article
Microbiology
Susan Joseph, Joseph Aduse-Opoku, Ahmed Hashim, Eveliina Hanski, Ricarda Streich, Sarah C. L. Knowles, Amy B. Pedersen, William G. Wade, Michael A. Curtis
Summary: A curated murine oral microbiome database has been constructed, containing a collection of nearly full-length 16S rRNA gene sequences from cultured isolates and draft genomes from representative taxa. The database comprises 103 mouse oral taxa (MOT) spanning four phyla, including 12 novel undescribed species-level taxa. Key observations include the low diversity and predominantly culturable nature of the laboratory mouse oral microbiome, as well as the identification of three major murine-specific oral bacterial lineages.
Article
Immunology
A. Hashim, A. Alsam, M. A. Payne, J. Aduse-Opoku, M. A. Curtis, S. Joseph
Summary: Neutrophils play a crucial role in regulating the oral microbiota, with their deficiency predisposing individuals to periodontal disease. Without neutrophils, there is a significant shift in the oral microbiome composition, leading to increased periodontal bone pathology. However, transferring the oral microbiome of neutrophil-deficient mice back to wild-type mice can restore the microbiome composition and prevent pathology.
INFECTION AND IMMUNITY
(2021)
Review
Agriculture, Dairy & Animal Science
Lihan Wang, Stephen J. Forsythe, Xinyan Yang, Shiqian Fu, Chaoxin Man, Yujun Jiang
Summary: Cronobacter genus includes food-borne pathogens that can cause severe infections in infants, with a high mortality rate. Advanced detection technology and analysis of high-frequency species from different sources can aid in targeted control of Cronobacter. The bacteria's resistance to environmental stresses and the development of new control techniques highlight the importance of strict monitoring and prevention throughout food processing and storage.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Dentistry, Oral Surgery & Medicine
S. Joseph, M. Carda-Dieguez, J. Aduse-Opoku, A. Alsam, A. Mira, M. A. Curtis
Summary: Periodontal disease leads to changes in cellular profiles and biological activities of both the subgingival microbiome and host tissues. Analysis of a murine periodontal disease model using metatranscriptomics revealed differential expression of 3,468 host transcripts, with 76% being overexpressed in periodontitis. Disease was associated with alterations in immune and inflammatory genes and pathways, as well as cellular/metabolic processes and biological regulation. These metatranscriptome data provide insights into gene expression patterns in periodontal disease and can be used for future functional studies.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Microbiology
Veronika Kadlicekova, Michal Kajsik, Katarina Soltys, Tomas Szemes, Livia Slobodnikova, Lucia Janosikova, Zuzana Hubenakova, Pauline Ogrodzki, Stephen Forsythe, Jan Turna, Hana Drahovska
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2018)
