Article
Genetics & Heredity
Xuefeng Wei, Xue Zhao, Xinyue Shan, Yunchang Zhu, Shuzhe Wang, Hong Chen, Hui Li, Yun Ma
Summary: This study found that miR-107 was highly expressed in bovine adipose tissue and showed a downregulation trend during adipocyte differentiation. Functionally, miR-107 was shown to regulate adipocyte differentiation and lipogenesis by directly targeting APOC2. These findings provide a theoretical and experimental basis for further research on the regulatory mechanisms of adipocyte differentiation and lipogenesis. Additionally, the high conservation of miR-107 among different species suggests its potential as a molecular target for the treatment of lipid-related diseases in the future.
Article
Biotechnology & Applied Microbiology
Wenjing Wu, Keke Liu, Zhongyu You, Jin Zhang
Summary: Using BGISEQ-500 sequencing technology, this study found that miR-196b-3p expression was 32-fold higher and miR-450b-3p expression was 8-fold lower in intramuscular adipocytes (IM) compared to subcutaneous adipocytes (SC) in pigs. Functional studies revealed that miR-196b-3p inhibits adipogenesis by targeting CD47 via the AMPK signaling pathway, while miR-450b-3p promotes adipogenesis by targeting SIRT1 via the Wnt/β-catenin signaling pathway.
Article
Cell Biology
Min Chen, Soochi Kim, Liang Li, Sourav Chattopadhyay, Thomas A. Rando, Brian J. Feldman
Summary: Through single-cell RNA sequencing, a population of CD31- CD45- cells with a specific expression profile is discovered in the stromal vascular fraction of adipose tissue. This population is placed on an epistatic pathway upstream of preadipocytes and functional properties with broad implications, including physiological mechanisms regulating adipogenesis, are revealed.
Article
Biotechnology & Applied Microbiology
Shuaipeng Zhu, Binbin Zhang, Tingqi Zhu, Dongxue Wang, Cong Liu, Yixuan Liu, Yuehua He, Wenjie Liang, Wenting Li, Ruili Han, Donghua Li, Fengbin Yan, Yadong Tian, Guoxi Li, Xiangtao Kang, Zhuanjian Li, Ruirui Jiang, Guirong Sun
Summary: miR-128-3p inhibits intramuscular adipocyte differentiation in chickens by downregulating FDPS. The study provides a theoretical basis for the study of lipid metabolism and identifies a potential target for molecular breeding to improve meat quality.
Article
Endocrinology & Metabolism
Muhammad Bilal, Allah Nawaz, Tomonobu Kado, Muhammad Rahil Aslam, Yoshiko Igarashi, Ayumi Nishimura, Yoshiyuki Watanabe, Takahide Kuwano, Jianhui Liu, Hiroyuki Miwa, Takumi Era, Koichi Ikuta, Johji Imura, Kunimasa Yagi, Takashi Nakagawa, Shiho Fujisaka, Kazuyuki Tobe
Summary: The expansion of adipose tissue during obesity through the recruitment of newly generated adipocytes is metabolically healthy, particularly in the gonadal white adipose tissue of male mice receiving a high-fat diet. Contrary to previous findings, the inguinal white adipose tissue of male mice also shows a high potential for proliferation and differentiation of adipocyte progenitors, leading to hyperplasia in response to high-fat diet feeding.
MOLECULAR METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Jasmine A. Burrell, Jacqueline M. Stephens
Summary: KAT8 is essential for adipocyte differentiation, with its loss inhibiting the formation of adipocytes in the early stages. However, its absence does not significantly impact later stages of adipocyte differentiation. Further studies are needed to determine the specific functions and regulation of KAT8 in adipose tissue.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Review
Endocrinology & Metabolism
Tammy Ying, Rebecca A. Simmons
Summary: Understanding the development of adipocyte precursors is crucial for preventing obesity-related diseases. Recent studies using new techniques to analyze adipocyte precursors have revealed insights into their lineages and regulatory mechanisms.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Akiko Katano-Toki, Satoshi Yoshino, Yasuyo Nakajima, Takuya Tomaru, Ayaka Nishikido, Emi Ishida, Kazuhiko Horiguchi, Tsugumichi Saito, Atsushi Ozawa, Tetsurou Satoh, Masanobu Yamada
Summary: HELZ2 deficiency leads to resistance to obesity and NAFFL/NASH in mice, and the association of tyrosine-phosphorylated HELZ2 with SFPQ protein affects the expression levels of key factors for adipocyte differentiation, thus impacting the process of cell differentiation and maturation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Agriculture, Dairy & Animal Science
Zhongchang Hu, Jian Wu, Lihong Qin, Haiguo Jin, Yang Lv, Ran Zhang, Cheng Xiao, Yang Cao, Yumin Zhao
Summary: ALDH1A1 plays a significant regulatory role in the differentiation of preadipocyte cells in Yan Yellow Cattle. Modulation of ALDH1A1 gene expression can affect acetaldehyde oxidation and adipocyte development, with changes in lipid accumulation and triglyceride production observed in mature adipocytes. The protein expression levels of ALDH1A1 and adipogenic factors corresponded to the mRNA levels, indicating an important role of ALDH1A1 in fat cell growth and development.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Oncology
Archittapon Nokkeaw, Pannathon Thamjamrassri, Naphat Chantaravisoot, Pisit Tangkijvanich, Chaiyaboot Ariyachet
Summary: The long non-coding RNA H19 plays a significant role in hepatocellular carcinoma (HCC), promoting cell growth and cell cycle progression by acting as a molecular sponge for miR-107 to upregulate CDK6 expression.
Article
Cell Biology
Milica Vranic, Fozia Ahmed, Susanne Hetty, Assel Sarsenbayeva, Vitor Ferreira, Giovanni Fanni, Angela M. Valverde, Jan W. Eriksson, Maria J. Pereira
Summary: We investigated the effects of second-generation antipsychotics (SGAs) on adipocyte differentiation and metabolism and found that aripiprazole (ARI) can impair adipocyte glucose uptake and increase fatty acid oxidation gene expression, while olanzapine (OLA) can increase leptin gene expression. These findings have important implications for understanding the metabolic effects of antipsychotic drugs and suggest that different SGAs may have different mechanisms of action.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2023)
Review
Chemistry, Medicinal
Dharmani Devi Murugan, Dharvind Balan, Pooi-Fong Wong
Summary: Obesity is a serious public health issue, and in addition to lifestyle changes and dietary modifications, pharmacotherapy is considered as an alternative strategy for treatment. Bioactive phytochemicals are being explored as a valuable source for new antiobesity agents, but further research is needed to establish their clinical efficacy.
PHYTOTHERAPY RESEARCH
(2021)
Article
Allergy
Yi Xiong, Yani Luo, Yuwen Ting, Jiana Li, Rongchang Chen, Fei Shi
Summary: This study aimed to detect differentially expressed microRNAs in the serum of asthma patients and airway smooth muscle cells (ASMCs) of asthmatic mice, exploring their role in the airway remodeling of asthma. The expression of miR-107 was down-regulated in both mild and moderate-severe asthma patients and ASMCs of asthmatic mice. Up-regulating miR-107 suppressed ASMCs' proliferation by targeting Cdk6 and the phosphorylation level of Rb, and also inhibited ASMC migration by targeting Cdk6.
JOURNAL OF ASTHMA AND ALLERGY
(2023)
Article
Agriculture, Dairy & Animal Science
Zhongchang Hu, Jian Wu, Lihong Qin, Haiguo Jin, Yang Cao, Yumin Zhao
Summary: The study revealed that the expression levels of the IGFBP7 gene are specifically regulated during the adipocyte differentiation in Yan Yellow Cattle. Overexpression and RNA interference of the IGFBP7 gene had significant effects on lipid accumulation and triglyceride production in mature adipocytes.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Xinhao Ma, Xinran Yang, Dianqi Zhang, Wenzhen Zhang, Xiaoyu Wang, Kuncheng Xie, Jie He, Chugang Mei, Linsen Zan
Summary: This study conducted a comprehensive analysis of long non-coding RNAs (lncRNAs) in bovine intramuscular adipocytes during adipogenesis, identifying a total of 7035 lncRNAs, including 3396 novel lncRNAs. One particular lncRNA, BIANCR, was found to play a crucial regulatory role in adipogenesis through the ERK1/2 signaling pathway.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Vesa M. Olkkonen
Summary: Oxysterol-binding protein-related proteins (ORPs) are a family of intracellular lipid binding/transfer proteins involved in lipid transport within cells. They have the ability to bind phosphatidylinositol polyphosphates (PIPs). Dysregulation of certain ORP family members, such as ORP3, -4, -5, and -8, is implicated in cancer growth through their effects on signaling events, protein interactions, and cellular lipid transport. This review discusses the functional evidence of ORPs in cancer growth and explores the potential of targeting ORPs for anti-cancer therapy.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Julia Zinngrebe, Barbara Moepps, Thomas Monecke, Peter Gierschik, Ferdinand Schlichtig, Thomas F. E. Barth, Gudrun Strauss, Elena Boldrin, Carsten Posovszky, Ansgar Schulz, Ortraud Beringer, Eva Rieser, Eva-Maria Jacobsen, Myriam Ricarda Lorenz, Klaus Schwarz, Ulrich Pannicke, Henning Walczak, Dierk Niessing, Catharina Schuetz, Pamela Fischer-Posovszky, Klaus-Michael Debatin
Summary: This article describes an atypical clinical manifestation of OTULIN-related autoinflammatory syndrome (ORAS) caused by new compound heterozygous variants (c.258G>A (p.M86I)/c.500G>C (p.W167S)) in the OTULIN gene. The study found that these variants compromised the binding of OTULIN to linear ubiquitin, leading to the development of the disease.
EMBO MOLECULAR MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Vesa M. Olkkonen, Elina Ikonen
Summary: This review summarizes the role of OSBP family members in facilitating cholesterol trafficking within the endocytic pathway, particularly at membrane contact sites within the cell. ORP1 isoforms have been shown to mediate cholesterol transport between the endoplasmic reticulum and late endosomes/lysosomes, while ORP2 plays a role in transferring cholesterol between late endosomes and recycling endosomes. These processes involve lipid exchange and contribute to regulating cellular signaling pathways.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maria A. Ahonen, Marcus Horing, Van Dien Nguyen, Sami Qadri, Juuso H. Taskinen, Meghana Nagaraj, Martin Wabitsch, Pamela Fischer-Posovszky, You Zhou, Gerhard Liebisch, P. A. Nidhina Haridas, Hannele Yki-Jarvinen, Vesa M. Olkkonen
Summary: This study found that thyroid hormone responsive protein (THRSP) is regulated by insulin in human adipose tissue and adipocytes. Insulin resistance leads to downregulation of THRSP expression. Silencing of THRSP results in impaired mitochondrial function and disrupted lipid metabolism.
MOLECULAR MEDICINE
(2022)
Review
Endocrinology & Metabolism
Anke Hinney, Antje Koerner, Pamela Fischer-Posovszky
Summary: Obesity is a complex and multifactorial disease that starts in early childhood and has lifelong consequences. It is influenced by both genetic predisposition and environmental factors. While polygenic obesity is common and has small effects, rare monogenic obesity syndromes are caused by specific pathogenic variants in a single gene with large effects. These genes are mainly involved in the central nervous regulation of body weight, such as the leptin-melanocortin pathway. Genetic testing is recommended for patients with rapid weight gain in infancy and other clinical suggestive features, as novel pharmacological treatments targeting the central melanocortin pathway are emerging. Early identification of syndromic obesity patients can lead to appropriate treatment, preventing obesity-related complications and stigma.
NATURE REVIEWS ENDOCRINOLOGY
(2022)
Review
Endocrinology & Metabolism
Daniel Tews, Rolf E. Brenner, Reiner Siebert, Klaus-Michael Debatin, Pamela Fischer-Posovszky, Martin Wabitsch
Summary: SGBS cells, derived from adipose tissue, are widely used as a cell model for studying human adipogenesis and adipocyte biology. These cells can proliferate and differentiate into adipocytes for over 50 population doublings, providing an almost unlimited source of adipocyte progenitor cells. Research using SGBS cells has resulted in numerous publications covering various aspects of adipogenesis and adipocyte biology.
INTERNATIONAL JOURNAL OF OBESITY
(2022)
Editorial Material
Endocrinology & Metabolism
Julia Zinngrebe, Pamela Fischer-Posovszky
Summary: Inosine, released from apoptotic brown adipocytes, has been identified as an inducer of thermogenic adipose tissue, uncovering a novel mechanism of adipose tissue plasticity.
NATURE REVIEWS ENDOCRINOLOGY
(2022)
Article
Multidisciplinary Sciences
Luisa Sophie Rajcsanyi, Yiran Zheng, Pamela Fischer-Posovszky, Martin Wabitsch, Johannes Hebebrand, Anke Hinney
Summary: Homozygosity for pathogenic variants in the leptin gene leads to severe early-onset obesity. The prevalence of these variants in the general population is low, with variations among different populations.
Article
Biochemistry & Molecular Biology
Juuso H. Taskinen, Hanna Ruhanen, Silke Matysik, Reijo Kakela, Vesa M. Olkkonen
Summary: This study elucidated the effects of inhibiting OSBP on endothelial cells, including changes in the lipidome, transcriptome, and disruption of in vitro angiogenic capacity. Thus, OSBP plays a crucial regulatory role in endothelial cell lipid homeostasis and angiogenic capacity.
Meeting Abstract
Hematology
Alexandra Niedermayer, Stefanie Enzenmueller, Felix Seyfried, Daniel Tews, Pamela Fischer-Posovszky, Ulrich Rupp, Paul Walther, Klaus-Michael Debatin, Luder Hinrich Meyer
Article
Biochemistry & Molecular Biology
Maja Radulovic, Eva Maria Wenzel, Sania Gilani, Lya K. K. Holland, Alf Hakon Lystad, Santosh Phuyal, Vesa M. Olkkonen, Andreas Brech, Marja Jaattela, Kenji Maeda, Camilla Raiborg, Harald Stenmark
Summary: This study reveals an additional mechanism for lysosome repair that is activated independently of the ESCRT machinery. It shows that damaged lysosomes rapidly form contacts with the endoplasmic reticulum (ER), mediated by ER proteins VAPA/B, which provide lipids for membrane repair. The study also highlights the importance of lysosomal phosphatidylserine and cholesterol accumulation, as well as the generation and removal of PtdIns4P in this repair response.
Article
Biochemistry & Molecular Biology
Lena Molitor, Melina Klostermann, Sabrina Bacher, Juliane Merl-Pham, Nadine Spranger, Sandra Burczyk, Carolin Ketteler, Ejona Rusha, Daniel Tews, Anna Pertek, Marcel Proske, Anke Busch, Sarah Reschke, Regina Feederle, Stefanie M. Hauck, Helmut Blum, Micha Drukker, Pamela Fischer-Posovszky, Julian Koenig, Kathi Zarnack, Dierk Niessing
Summary: The RNA-binding protein PURA is predominantly located in the cytoplasm, where it binds to thousands of mRNAs. Reduced levels of PURA affect the formation and composition of P-bodies, leading to dysregulation of RNA processing. This study proposes PURA Syndrome as a new model for studying the connection between P-body-associated RNA regulation and neurodevelopmental disorders.
NUCLEIC ACIDS RESEARCH
(2023)
Editorial Material
Cell Biology
Jan Tuckermann, Pamela Fischer-Posovszky
Summary: Immune functions are influenced by nutritional state. A recent publication in Immunity by Janssen et al. revealed that fasting-induced glucocorticoid release leads to the movement of monocytes from blood into the bone marrow. Upon refeeding, these chronologically older monocytes are released again and have detrimental effects during bacterial infection.
Meeting Abstract
Endocrinology & Metabolism
Julian Roos, Hang Wu, Taner Pula, Daniel Tews, Martin Wabitsch, Klaus-Michael Debatin, Pamela Fischer-Posovszky
HORMONE RESEARCH IN PAEDIATRICS
(2022)
Meeting Abstract
Endocrinology & Metabolism
Daniel Tews, Julian Roos, Gordon Winter, Ambros Beer, Volker Rasche, Elly Nedivi, Klaus-Michael Debatin, Martin Wabitsch, Pamela Fischer-Posovszky
HORMONE RESEARCH IN PAEDIATRICS
(2022)
Article
Cell Biology
Karina A. Pena, Sofya Savransky, Breanna Lewis
Summary: Compartmentalization of GPCR signaling is an emerging topic that emphasizes the importance of spatial bias in signaling for physiological relevance. PTH1R was the first GPCR discovered to signal via cAMP from endosomes, challenging the conventional model of GPCR signaling. The location of cAMP generation determines the physiological outcomes of GPCR signaling.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Cheng-Xu Ma, Xiao-Ni Ma, Jin-Jin Liu, Cong-Hui Guan, Ying-Dong Li, Nan Zhao, Didac Mauricio, Song-Bo Fu
Summary: The downregulation of primary cilia (PCs) due to BRAFV600E mutation contributes to the aggressiveness and lymph node metastasis of Papillary Thyroid Cancer (PTC).
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Kejun Wu, Cuihua Huang, Wenrong Zheng, Yubin Wu, Qintao Huang, Menghua Lin, Ruonan Gao, Liqin Qi, Guanlian He, Xiaoying Liu, Xiaohong Liu, Linxi Wang, Zhou Chen, Libin Liu
Summary: Recurrent non-severe hypoglycemia in patients with diabetes is associated with cognitive impairment. This study found that this condition is associated with reduced mitophagy in the hippocampus, leading to mitochondrial dysfunction and neurological impairment.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Yifei Lv, Yizhou Huang, Huiyu Fan, Yunxiu Zhao, Linjuan Ma, Yibing Lan, Chunming Li, Peiqiong Chen, Zheng Lou, Jianhong Zhou
Summary: Before menopause, females have a lower incidence of cardiovascular disease than age-matched males, possibly due to the protective effects of sex hormones. 17 beta-E2 inhibits THBS1 expression, preventing cell senescence and apoptosis, and counteracts oxidative stress by suppressing the TGF-beta/Smad signaling pathway.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Ana C. L. Camargo, Flavia B. Constantino, Sergio A. A. Santos, Ketlin T. Colombelli, Luiz M. F. Portela, Matheus N. Fioretto, Luisa A. Barata, Guilherme T. Valente, Carlos S. Moreno, Luis A. Justulin
Summary: This study examined the effects of maternal malnutrition on the transcriptomic landscape of the ventral prostate in rats. It found that changes in molecular pathways related to cellular development and tissue morphogenesis were associated with maternal malnutrition. The Abcg1 gene was found to be deregulated in both malnourished rats and prostate cancer models and patients.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Sandra Romero-Cordoba, Mayel Chirinos, Nancy Noyola-Martinez, Nayeli Torres-Ramirez, Mitzi Garcia-Olivares, Juan Pablo Aragon-Hernandez, Ixchel Ramirez-Camacho, Rosa Zuniga, Fernando Larrea, Ali Halhali, David Barrera
Summary: This study analyzed the effects of calcitriol, TGF-131, and their combination on human trophoblast cells. The results showed that the combination treatment modified the transcriptional landscape and mainly affected the storage, activity, and metabolism of lipids, which may have an impact on placental development.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Traver J. Wright, Richard B. Pyles, Melinda Sheffield-Moore, Rachel R. Deer, Kathleen M. Randolph, Kristen A. Mcgovern, Christopher P. Danesi, Charles R. Gilkison, Weston W. Ward, Jayson A. Vargas, Peyton A. Armstrong, Sarah E. Lindsay, Mohammed F. Zaidan, Justin Seashore, Tamara L. Wexler, Brent E. Masel, Randall J. Urban
Summary: This study investigates the persistent neurologic symptoms in COVID-19 patients after recovery and explores the association between these symptoms and disrupted growth hormone secretion and gastrointestinal discomfort.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Li-Li Chen, Ya-Qin Li, Zhi-Hui Kang, Xuan Zhang, Su-Yan Gu, Na Wang, Xue-Yan Shen
Summary: Defects in trophoblastic EMT caused by dysregulation of circTNRC18's interaction with LIN28A play a vital role in the development of preeclampsia. LIN28A overexpression suppresses circTNRC18-mediated inhibition of trophoblast migration, invasion, and EMT, while LIN28A knockdown promotes them. Furthermore, circTNRC18 regulates the intracellular distribution of LIN28A and the expression of insulin-like growth factor II, affecting cell migration and invasion. Targeting the circTNRC18-LIN28A regulatory axis may provide a novel treatment approach for preeclampsia.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
