Review
Cell Biology
Laura Prendergast, Danny Reinberg
Summary: Recent advances in chromatin and epigenetics research have highlighted the significance of core histones, histone variants, and their post-translational modifications in regulating chromatin structure. The role of linker histone H1, which was previously understudied, has come into focus due to its evolutionary conservation, variability in PTMs, and unique functions across different cell types. Contrary to previous assumptions, histone H1 variants are now recognized for their independent roles in various chromatin-based processes, challenging the traditional view of their function solely in chromatin compaction and transcriptional repression.
GENES & DEVELOPMENT
(2021)
Review
Microbiology
Yiling Lai, Lili Wang, Weilu Zheng, Sibao Wang
Summary: Fungal pathogens use transcriptional reprogramming to regulate gene expression in response to changing host environments and defenses. Histone post-translational modifications play a crucial role in this process and are involved in fungal development, pathogenicity, and interactions with hosts.
Article
Biochemical Research Methods
Eva Hoellmueller, Katharina Greiner, Simon M. Kienle, Martin Scheffner, Andreas Marx, Florian Stengel
Summary: Linker histone H1 plays a key role in chromatin organization and maintenance. Using site-specifically mono- and di-acetylated linker histone H1.2 generated by genetic code expansion, this study identified and characterized the acetylation-dependent cellular interactome of H1.2, demonstrating a role in modulating protein-protein interactions. Among the interacting partners identified are translational initiation factors and transcriptional regulators.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Arsheed H. Sheikh, Kashif Nawaz, Naheed Tabassum, Marilia Almeida-Trapp, Kiruthiga G. Mariappan, Hanna Alhoraibi, Naganand Rayapuram, Manuel Aranda, Martin Groth, Heribert Hirt
Summary: The mutants of Arabidopsis H1 histones, H1.1 and H1.2 double mutants and H1.1, H1.2, and H1.3 triple mutants, were found to be resistant to Pseudomonas syringae and Botrytis cinerea infections. Transcriptome analysis revealed that H1s play a crucial role in regulating the expression of defense genes during pathogen challenge. Overall, the study demonstrates the role of H1 as a molecular gatekeeper in modulating the chromatin landscape of defense genes during plant-pathogen interaction.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Kaiyuan Wang, Mario Escobar, Jing Li, Barun Mahata, Jacob Goell, Spencer Shah, Madeleine Cluck, Isaac B. Hilton
Summary: This study systematically compared different dCas9-based transcriptional activators and found that their expression levels and transactivation efficacies vary in different human cell types. The study also showed that these activators can induce the production of enhancer RNAs, which are positively correlated with downstream mRNA expression. Additionally, the study demonstrated the existence of intrinsic transcriptional and epigenetic reciprocity between human enhancers and promoters.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Plant Sciences
Rocio Nunez-Vazquez, Benedicte Desvoyes, Crisanto Gutierrez
Summary: Plants have developed various mechanisms to adapt to abiotic stresses, which involve transcriptional regulation and chromatin modifications. Understanding and manipulating these regulatory networks are crucial for enhancing crop resilience and performance against stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Chao Zhou, Hanlin Zhou, Xueping Ma, Huilan Yang, Ping Wang, Guodong Wang, Lanlan Zheng, Yonghong Zhang, Xiaoyun Liu
Summary: This study reveals the genome-wide distribution of six histone modifications in sorghum and finds that the codecoration of H3K4me3 and H3K36me3 is negatively associated with the production of lincRNAs. Both H3K4me3 and H3K36me3 are involved in the negative-going regulation of plant lincRNA and mRNA genes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Plant Sciences
Jun-Li Wang, Dong-Wei Di, Pan Luo, Li Zhang, Xiao-Feng Li, Guang-Qin Guo, Lei Wu
Summary: This review focuses on the molecular mechanisms through which epigenetic modifications regulate auxin biosynthesis, demonstrating that complex signaling pathways affect gene expression and subsequently protein production.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Qiong Wu, Anders E. Berglund, Arnold B. Etame
Summary: Glioblastoma is highly resistant to standard therapies and the durability of response to the best chemotherapy agent, Temozolomide, is often short-lived due to tumor resistance. The need for therapies that can provide synergy to chemoradiation is urgent, as adaptive resistance evolution in GBM is facilitated through treatment-induced epigenetic modifications. Understanding and targeting these epigenetic modifications associated with GBM resistance is a top priority.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Jaemyung Choi, David B. Lyons, Daniel Zilberman
Summary: Cells adapt to different roles by turning different groups of genes on and off, which is controlled by creating regions of active and inactive DNA. In plants such as Arabidopsis thaliana, two types of inactive DNA, E and H, silence transposons through methylation. The involvement of histone H1 in blocking DNA methylation in H regions demonstrates how different DNA silencing processes are focused onto specific genetic regions within cells.
Review
Plant Sciences
Mu Xiao, Jinbiao Wang, Fang Xu
Summary: Plants dynamically regulate their gene expression in response to challenging environments through histone methylation. Osmotic stress can cause significant changes in histone methylation profiles and genome-wide transcriptional reprogramming. However, the precise mechanism of how stress-inducible genes are epigenetically regulated by histone methylation remains largely unknown. This review summarizes recent findings on the interaction between histone (de)methylation and osmotic stress, focusing on the effects of stress on histone methylation profiles and how histone methylation optimizes plant performance under stress conditions.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Oncology
Francisco Gimeno-Valiente, Gerardo Lopez-Rodas, Josefa Castillo, Luis Franco
Summary: This review focuses on the interconnections between epigenetics and alternative splicing in the development of cancer. It discusses the mechanisms involved in these interconnections and the potential diagnostic and therapeutic tools that can be derived from them. The reversible nature of epigenetic alterations and the possibility of correcting aberrant alternative splicing offer promising therapeutic possibilities for cancer treatment.
Article
Genetics & Heredity
Milad Shirvaliloo
Summary: The article summarizes the importance of histones and their role in gene expression regulation. It highlights the significance of recent research on histone modifications published in Epigenomics since 2020.
Review
Microbiology
Yiman Li, Zhihui Song, Ente Wang, Liming Dong, Jie Bai, Dong Wang, Jinyan Zhu, Chao Zhang
Summary: Invasive aspergillosis is a significant cause of death in patients with hematological malignancies and transplant recipients. Antifungal drug resistance has become a concern in managing infections, and understanding histone posttranslational modifications may provide insights for developing new antifungal drugs.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Sonali Parida, Amruta Mohapatra, Sweta Das, Pramoda Kumar Sahoo
Summary: This study focused on the role of linker histone H1 (LHH1) in the ontogeny and innate immunity of the Indian major carp species rohu. The expression of LHH1 was found to be significantly increased during early development and varied in response to different infection models. However, a synthetic peptide derived from LHH1 did not possess antibacterial activity.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Medical Laboratory Technology
Giovanni Rossi, Antonietta Pia Falcone, Maria Marta Minervini, Giovanni Pio De Cillis, Chiara De Waure, Leuconoe Grazia Sisti, Vincenzo Giambra, Daniela Valente, Vincenzo Chiello, Potito Rosario Scalzulli, Angelo Michele Carella, Nicola Cascavilla
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2019)
Article
Genetics & Heredity
Silvia Morlino, Annalucia Carbone, Marco Ritelli, Carmela Fusco, Vincenzo Giambra, Grazia Nardella, Angelantonio Notarangelo, Patrizio Panelli, Gianluigi Mazzoccoli, Nicoletta Zoppi, Paola Grammatico, Emma M. Wade, Marina Colombi, Marco Castori, Lucia Micale
Article
Medical Laboratory Technology
Giovanni Rossi, Vincenzo Giambra, Maria M. Minervini, Chiara De Waure, Silvia Mancinelli, Michele Ciavarella, Nicola P. Sinisi, Potito R. Scalzulli, Angelo M. Carella, Nicola Cascavilla
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2020)
Article
Neurosciences
C. Grasselli, A. Carbone, P. Panelli, V Giambra, M. Bossi, G. Mazzoccoli, L. De Filippis
MOLECULAR NEUROBIOLOGY
(2020)
Article
Anthropology
Domenico Frezza, Cristina Martinez-Labarga, Vincenzo Giambra, Eliseo Serone, Giuseppina Scano, Olga Rickards, Pietro D'Addabbo, Andrea Novelletto
AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Lucia Micale, Silvia Morlino, Tommaso Biagini, Annalucia Carbone, Carmela Fusco, Marco Ritelli, Vincenzo Giambra, Nicoletta Zoppi, Grazia Nardella, Angelantonio Notarangelo, Annalisa Schirizzi, Gianluigi Mazzoccoli, Paola Grammatico, Emma M. Wade, Tommaso Mazza, Marina Colombi, Marco Castori
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2020)
Article
Oncology
Santina Venuto, Laura Monteonofrio, Flora Cozzolino, Maria Monti, Irene Appolloni, Tommaso Mazza, Diana Canetti, Vincenzo Giambra, Patrizio Panelli, Carmela Fusco, Gabriella Maria Squeo, Anna Irma Croce, Pietro Pucci, Paolo Malatesta, Silvia Soddu, Giuseppe Merla, Lucia Micale
Review
Oncology
Francesco Tamiro, Andrew P. Weng, Vincenzo Giambra
Summary: Leukemia-initiating cells (LIC) are unique cells in different types of leukemia that have self-renewing capabilities and produce tumors, which are functionally distinct from bulk leukemia cells. Current conventional treatments are not effective in eliminating LICs, hence innovative therapeutics targeting LICs hold promise for developing an effective cure for ALL.
Review
Oncology
Kalpana K. Bhanumathy, Amrutha Balagopal, Frederick S. Vizeacoumar, Franco J. Vizeacoumar, Andrew Freywald, Vincenzo Giambra
Summary: Protein phosphorylation is a key regulatory mechanism controlling cellular responses, catalysed by members of the protein kinase superfamily. Tyrosine kinases have been extensively studied for their roles in human malignancies, leading to the development of targeted therapies. Various tyrosine kinases, both receptor and nonreceptor types, play critical roles in the pathogenesis and drug resistance of leukemia, highlighting their potential as therapeutic targets.
Article
Immunology
Mattia Colucci, Elisabetta De Santis, Beatrice Totti, Mattia Miroballo, Francesco Tamiro, Giovanni Rossi, Ada Piepoli, Gabriella De Vincentis, Antonio Greco, Alessandra Mangia, Rossella Cianci, Lazzaro Di Mauro, Giuseppe Miscio, Vincenzo Giambra
Summary: This study analyzed antibody levels, antigen-specific B cells, and total T and NK cell subsets in subjects after BNT162b2 vaccination, finding significant increases in these components in former COVID-19 patients and highly responsive individuals. Additionally, genetic variants in the human immunoglobulin constant-gene locus were identified to be associated with response levels to the vaccine.
Article
Biochemistry & Molecular Biology
Francesca Sansico, Mattia Miroballo, Daniele Salvatore Bianco, Francesco Tamiro, Mattia Colucci, Elisabetta De Santis, Giovanni Rossi, Jessica Rosati, Lazzaro Di Mauro, Giuseppe Miscio, Tommaso Mazza, Angelo Luigi Vescovi, Gianluigi Mazzoccoli, Vincenzo Giambra
Summary: The research conducted single-cell RNA sequencing analysis revealed that patients with COVID-19 exhibited an increase in COVID-specific surface markers in different subsets of immune cells, while the expression of cell receptors was reduced in response to the viral infection. These results highlight the presence of novel immunoreceptors in COVID-19 patients affecting immune functionality.
Article
Immunology
Vincenzo Giambra, Annarita Valeria Piazzolla, Giovanna Cocomazzi, Maria Maddalena Squillante, Elisabetta De Santis, Beatrice Totti, Chiara Cavorsi, Francesco Giuliani, Nicola Serra, Alessandra Mangia
Summary: This study assessed the humoral and cellular immune response of cirrhotic patients after receiving the SARS-CoV-2 vaccine. The results showed that cirrhotic patients had slower and suboptimal immune responses compared to the control group, but the rates of breakthrough infections were similar between the two groups.
Article
Biochemistry & Molecular Biology
Tommaso Colangelo, Patrizio Panelli, Francesco Mazzarelli, Francesco Tamiro, Valentina Melocchi, Elisabetta De Santis, Roberto Cuttano, Orazio Palumbo, Giovanni Rossi, Fabrizio Bianchi, Vincenzo Giambra
Summary: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive T-cell malignancy. Recent studies have shown that T-ALL-secreted small extracellular vesicles (sEV) contain NOTCH1-dependent microRNAs (EV-miRs), which regulate oncogenic pathways and promote the expansion and survival of highly proliferative cell subsets, thereby altering the cell heterogeneity and dynamics of leukemia.
Article
Biochemistry & Molecular Biology
Rossella Cianci, Maria Grazia Massaro, Elisabetta De Santis, Beatrice Totti, Antonio Gasbarrini, Giovanni Gambassi, Vincenzo Giambra
Summary: This brief report aimed to assess the correlation between Remdesivir (RDV) therapy and variation in lymphocyte subpopulations. The results showed significant changes in lymphocyte subpopulations after RDV therapy, with an increase in B lymphocytes and plasmablasts, and a reduction in cytotoxic T lymphocytes. There was also a decrease in regulatory T cells and an increase in circulating monocytes. Patients with different disease severity showed different lymphocyte count changes after RDV treatment. These findings may suggest a potential regulatory role of RDV treatment in the immune response against the virus and the cytokine storm observed in patients with more severe disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Vincenzo Giambra, Danilo Pagliari, Pierluigi Rio, Beatrice Totti, Chiara Di Nunzio, Annalisa Bosi, Cristina Giaroni, Antonio Gasbarrini, Giovanni Gambassi, Rossella Cianci
Summary: Inflammatory bowel diseases (IBDs) are characterized by chronic inflammation and an increased risk of colorectal cancer (CRC) development. The imbalance between the immune system and gut microbiota leads to changes in gut permeability, triggering inflammatory responses. This review provides an overview of pattern recognition receptors (PRRs), including their recognition mechanisms, signaling pathways, and contributions to immune responses.