Comprehensive and Reproducible Phosphopeptide Enrichment Using Iron Immobilized Metal Ion Affinity Chromatography (Fe-IMAC) Columns

Characterization of Biases in Phosphopeptide Enrichment by Ti4+-Immobilized Metal Affinity Chromatography and TiO2 Using a Massive Synthetic Library and Human Cell Digests

(2014) Lucrece Matheron et al.   ANALYTICAL CHEMISTRY

Proteomic analysis of phosphorylation in cancer

(2014) Benjamin Ruprecht et al.   Expert Review of Proteomics

Mass-spectrometry-based draft of the human proteome

(2014) Mathias Wilhelm et al.   NATURE

Sequential Phosphoproteomic Enrichment through Complementary Metal-Directed Immobilized Metal Ion Affinity Chromatography

(2013) Chia-Feng Tsai et al.   ANALYTICAL CHEMISTRY

Combination of Multistep IMAC Enrichment with High-pH Reverse Phase Separation for In-Depth Phosphoproteomic Profiling

(2013) Xiao-Shan Yue et al.   JOURNAL OF PROTEOME RESEARCH

Hydrophilic Strong Anion Exchange (hSAX) Chromatography for Highly Orthogonal Peptide Separation of Complex Proteomes

(2013) Maria Stella Ritorto et al.   JOURNAL OF PROTEOME RESEARCH

Rapid and Deep Human Proteome Analysis by Single-dimension Shotgun Proteomics

(2013) Mohammad Pirmoradian et al.   MOLECULAR & CELLULAR PROTEOMICS

The One Hour Yeast Proteome

(2013) Alexander S. Hebert et al.   MOLECULAR & CELLULAR PROTEOMICS

A large synthetic peptide and phosphopeptide reference library for mass spectrometry–based proteomics

(2013) Harald Marx et al.   NATURE BIOTECHNOLOGY

Integrated proteomic analysis of post-translational modifications by serial enrichment

(2013) Philipp Mertins et al.   NATURE METHODS

DMSO enhances electrospray response, boosting sensitivity of proteomic experiments

(2013) Hannes Hahne et al.   NATURE METHODS

Robust phosphoproteome enrichment using monodisperse microsphere–based immobilized titanium (IV) ion affinity chromatography

(2013) Houjiang Zhou et al.   Nature Protocols

Combinatorial Use of Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC) and Strong Cation Exchange (SCX) Chromatography for In-Depth Phosphoproteome Analysis

(2012) Mostafa Zarei et al.   JOURNAL OF PROTEOME RESEARCH

Toward a Comprehensive Characterization of a Human Cancer Cell Phosphoproteome

(2012) Houjiang Zhou et al.   JOURNAL OF PROTEOME RESEARCH

TiSH — a robust and sensitive global phosphoproteomics strategy employing a combination of TiO2, SIMAC, and HILIC

(2012) Kasper Engholm-Keller et al.   Journal of Proteomics

Analysis of protein mixtures from whole-cell extracts by single-run nanoLC-MS/MS using ultralong gradients

(2012) Thomas Köcher et al.   Nature Protocols

Complementary Fe3+- and Ti4+-immobilized metal ion affinity chromatography for purification of acidic and basic phosphopeptides

(2012) Alan Chuan-Ying Lai et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Rapid and Reproducible Single-Stage Phosphopeptide Enrichment of Complex Peptide Mixtures: Application to General and Phosphotyrosine-Specific Phosphoproteomics Experiments

(2011) Arminja N. Kettenbach et al.   ANALYTICAL CHEMISTRY

More than 100,000 Detectable Peptide Species Elute in Single Shotgun Proteomics Runs but the Majority is Inaccessible to Data-Dependent LC−MS/MS

(2011) Annette Michalski et al.   JOURNAL OF PROTEOME RESEARCH

Comparison of ERLIC–TiO2, HILIC–TiO2, and SCX–TiO2for Global Phosphoproteomics Approaches

(2011) Mostafa Zarei et al.   JOURNAL OF PROTEOME RESEARCH

Characterization of a TiO2 enrichment method for label-free quantitative phosphoproteomics

(2011) Alex Montoya et al.   METHODS

Deep and Highly Sensitive Proteome Coverage by LC-MS/MS Without Prefractionation

(2011) Suman S. Thakur et al.   MOLECULAR & CELLULAR PROTEOMICS

Quantitative Chemical Proteomics Reveals New Potential Drug Targets in Head and Neck Cancer

(2011) Zhixiang Wu et al.   MOLECULAR & CELLULAR PROTEOMICS

Enhancing the Identification of Phosphopeptides from Putative Basophilic Kinase Substrates Using Ti (IV) Based IMAC Enrichment

(2011) Houjiang Zhou et al.   MOLECULAR & CELLULAR PROTEOMICS

Metal ion-mobilizing additives for comprehensive detection of femtomole amounts of phosphopeptides by reversed phase LC-MS

(2010) Joerg Seidler et al.   AMINO ACIDS

Optimized IMAC−IMAC Protocol for Phosphopeptide Recovery from Complex Biological Samples

(2010) Juanying Ye et al.   JOURNAL OF PROTEOME RESEARCH

RockerBox: Analysis and Filtering of Massive Proteomics Search Results

(2010) Henk W. P. van den Toorn et al.   JOURNAL OF PROTEOME RESEARCH

The phosphoproteomics data explosion

(2009) Simone Lemeer et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Effect of Peptide-to-TiO2Beads Ratio on Phosphopeptide Enrichment Selectivity

(2009) Qing-run Li et al.   JOURNAL OF PROTEOME RESEARCH

Fully Automatic Separation and Identification of Phosphopeptides by Continuous pH-Gradient Anion Exchange Online Coupled with Reversed-Phase Liquid Chromatography Mass Spectrometry

(2008) Jie Dai et al.   JOURNAL OF PROTEOME RESEARCH

The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry

(2008) Judit Villén et al.   Nature Protocols

Highly Robust, Automated, and Sensitive Online TiO2-Based Phosphoproteomics Applied To Study Endogenous Phosphorylation inDrosophila melanogaster

(2007) Martijn W. H. Pinkse et al.   JOURNAL OF PROTEOME RESEARCH