Article
Chemistry, Multidisciplinary
Irene Herrera-Gonzalez, Michel Thepaut, Elena M. Sanchez-Fernandez, Antonio di Maio, Corinne Vives, Javier Rojo, Jose M. Garcia Fernandez, Franck Fieschi, Pedro M. Nieto, Carmen Ortiz Mellet
Summary: Selective targeting of DC-SIGN and langerin may lead to the development of anti-infective agents. This study demonstrates that multivalent sp(2)-iminosugar-containing mannobioside analogs can achieve total DC-SIGN selectivity by leveraging the canonical binding mode towards high-mannose oligosaccharide ligands, acting as real biomimics.
CHEMICAL COMMUNICATIONS
(2022)
Article
Endocrinology & Metabolism
Xiaogang Li, Xi Wang, Haolong Li, Yongzhe Li, Ye Guo
Summary: The lipidomics profile of patients with Kallmann syndrome was found to be significantly different from healthy controls, with triacylglycerols, phosphatidylcholines, and phosphatidylethanolamine being identified as the main differential lipid species. This study suggests that TAGs, PCs, and PE may serve as promising biomarkers for KS diagnosis, representing the first report to analyze lipidomics in men with Kallmann syndrome.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Genetics & Heredity
Ye Guo, Xiaogang Li, Xi Wang, Haolong Li, Guoju Luo, Yongzhen Si, Xueyan Wu, Yongzhe Li
Summary: This study investigated the pathogenesis of PSIS through metabolomics and lipidomics profiling, and found that pregnenolones and L-saccharopine could serve as potential biomarkers for the diagnosis of PSIS.
ORPHANET JOURNAL OF RARE DISEASES
(2022)
Article
Oncology
Daniel J. Serie, Amanda A. Myers, Daniela A. Haehn, Alexander S. Parker, Essa M. Bajalia, Giovanni A. Gonzalez, Qiongyu Li, Maurice Yu Wong, Kaitlynn C. Moser, Bo Zhou, David D. Thiel
Summary: By using high-throughput glycoproteomics, we identified differential expression of certain glycopeptides associated with progression-free survival in patients with clear cell renal cell carcinoma (ccRCC). These findings suggest that glycoprotein-isoforms may serve as novel biomarkers for clinical diagnosis and prognosis of ccRCC.
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2022)
Article
Nutrition & Dietetics
Yong Huang, Xiude Li, Tengfei Zhang, Xueke Zeng, Meiling Li, Haowei Li, Hu Yang, Chenghao Zhang, Yu Zhu, Zhuang Zhang, Min Tang, Wanshui Yang
Summary: A study based on a nationwide representative sample in the US found that a healthy low-carbohydrate diet (LCD) is associated with lower levels of insulin, insulin resistance, inflammation, and triglycerides, as well as higher levels of high-density lipoprotein cholesterol. On the other hand, an unhealthy LCD is associated with higher levels of insulin, insulin resistance, inflammation, and low-density lipoprotein cholesterol.
BRITISH JOURNAL OF NUTRITION
(2023)
Article
Medicine, Research & Experimental
Ling He, Xingwu Wu, Rongye Wu, Ping Guo, Wenfeng He, Wanlei Sun, He Chen
Summary: This study revealed the expression changes of piRNAs in asthenozoospermia, providing clues for understanding the pathogenesis of this condition. Through bioinformatics analysis and experimental validation, differentially expressed piRNAs were found to be mainly associated with functions such as transcription, signal transduction, and cell differentiation. Four selected piRNAs were proven to have good diagnostic capability, and their combination further improved the diagnostic efficacy.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Obstetrics & Gynecology
Delnya Gholami, Farzane Amirmahani, Reza Salman Yazdi, Tahereh Hasheminia, Hossein Teimori
Summary: The study found that up-regulation of miR-182-5p, miR-192-5p, and miR-493-5p may serve as potential biomarkers for TZ. Additionally, the expression level of the CRISP3 glycosylated isoform was significantly lower in TZ patients compared to those with normal sperm. These results suggest a significant role of CRISP3 protein and miRs in male infertility.
REPRODUCTIVE SCIENCES
(2021)
Article
Geriatrics & Gerontology
Chiara Zecca, Giuseppe Pasculli, Rosanna Tortelli, Maria Teresa Dell'Abate, Rosa Capozzo, Maria Rosaria Barulli, Roberta Barone, Miriam Accogli, Serena Arima, Alessio Pollice, Vincenzo Brescia, Giancarlo Logroscino
Summary: The study explores the influence of age and other factors on A beta(1-42) plasma levels in cognitively and neurologically normal individuals. Age and cholesterol were significantly associated with A beta(1-42) plasma level, with only the association of age surviving post hoc adjustment for multiple comparisons. Further research is needed to understand the age-dependent variability of A beta levels across different age groups.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Environmental Sciences
Heng-Gui Chen, Bin Sun, Fuxin Lin, Ying-Jun Chen, Cheng-Liang Xiong, Tian-Qing Meng, Peng Duan, Carmen Messerlian, Zhijian Hu, An Pan, Weimin Ye, Yi-Xin Wang
Summary: The study found a positive association between selenium levels in feed and sperm quality in healthy Chinese men. It also found a negative association between selenium levels and sperm mitochondrial DNA copy number, and sperm mitochondrial DNA copy number mediated the effects of selenium on sperm quality.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Pharmacology & Pharmacy
Teresa L. L. Krammer, Marietta Kollars, Paul A. A. Kyrle, Matthias Hackl, Sabine Eichinger, Ludwig Traby
Summary: This study investigates the potential of plasma miRNAs as biomarkers for monitoring antiplatelet treatment. The results show that platelet-enriched miRNAs in the circulation are significantly reduced within 2 hours after P2Y12-mediated platelet inhibition, and higher baseline levels of these miRNAs are associated with a greater reduction upon antiplatelet therapy. Furthermore, the study suggests that miRNAs may be carried by platelet-derived and procoagulant extracellular vesicles.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Vanessa Porkolab, Martin Lepsik, Stefania Ordanini, Alexander St John, Aline Le Roy, Michel Thepaut, Emanuele Paci, Christine Ebel, Anna Bernardi, Franck Fieschi
Summary: DC-SIGN functions as the coreceptor for SARSCoV-2 virus, and a glycomimetic ligand called Polyman26 inhibits DC-SIGN-dependent trans-infection. By designing dendrimer constructs that can engage two binding sites of the tetrameric DC-SIGN simultaneously, the researchers were able to achieve nanomolar affinity. This study opens up possibilities for developing new virus attachment blockers for different C-type lectin receptors.
ACS CENTRAL SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Mariaimmacolata Preiano, Serena Correnti, Tahreem Arshad Butt, Giuseppe Viglietto, Rocco Savino, Rosa Terracciano
Summary: Male infertility is a global health problem, and semen analysis may not be enough for a confident diagnosis. A reliable platform to detect infertility biomarkers is urgently needed. Mass spectrometry (MS)-based diagnostic tests have the potential to revolutionize pathology, microbiology, and laboratory medicine. However, MS-biomarkers of male infertility remain a proteomic challenge. This review focuses on proteomics investigations using untargeted approaches for seminal fluid proteome profiling, aiming to discover MS-biomarkers of male infertility and classify infertility subtypes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Andrology
Hannah E. Lyons, Bridget M. Arman, Sarah A. Robertson, David J. Sharkey
Summary: This study systematically reviews the concentrations of immune regulatory cytokines in seminal plasma of healthy men and evaluates the impact of different quantification methodologies. The results show that the concentrations of many cytokines associated with fertility status vary greatly between studies, possibly due to different immunoassay methods and lack of validation for seminal plasma assessment. Accurate reference ranges for healthy men cannot be determined from the published data due to the large variation between studies.
Article
Biochemistry & Molecular Biology
Serena Correnti, Mariaimmacolata Preiano, Pierpaolo Murfone, Annalisa Fregola, Massimo Bitonti, Rocco Savino, Rosa Terracciano
Summary: This study applied the combination of dispersive solid phase extraction (d-SPE) and MALDI-TOF-MS to study human seminal plasma, aiming to reveal diagnostic characteristics for male infertility. The analysis found that specific peptides in seminal plasma could effectively distinguish fertile and non-fertile male donors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Xiaoxiao Han, Lin Hao, Zhenduo Shi, Ying Li, Liang Wang, Zhenbei Li, Qiang Zhang, Fangfang Hu, Yijuan Cao, Kun Pang, Zuobin Zhu
Summary: This study identified that extracellular vesicles tRF-Val-AAC-010 and tRF-Pro-AGG-003 are biomarkers for the diagnosis of non-obstructive azoospermia, with tRF-Val-AAC-010 showing potential as a non-invasive biomarker for predicting the presence of sperm in non-obstructive azoospermia testicular tissue.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Genetics & Heredity
Bobby G. Ng, Paulina Sosicka, Francois Fenaille, Annie Harroche, Sandrine Vuillaumier-Barrot, Mindy Porterfield, Zhi-Jie Xia, Shannon Wagner, Michael J. Bamshad, Marie-Christine Vergnes-Boiteux, Sophie Cholet, Stephen Dalton, Anne Dell, Thierry Dupre, Mathieu Fiore, Stuart M. Haslam, Yohann Huguenin, Tadahiro Kumagai, Michael Kulik, Katherine McGoogan, Caroline Michot, Deborah A. Nickerson, Tiffany Pascreau, Delphine Borgel, Kimiyo Raymond, Deepti Warad, Heather Flanagan-Steet, Richard Steet, Michael Tiemeyer, Nathalie Seta, Arnaud Bruneel, Hudson H. Freeze
Summary: The study identified a recurrent mutation in SLC37A4 causing a dominantly inherited congenital disorder of glycosylation characterized by coagulopathy and liver dysfunction with abnormal serum N-glycans. Liver-specific abnormalities in glycosylation were replicated in a CRISPR base-edited hepatoma cell line carrying the mutation. The mutant protein showed relocation to a non-Golgi compartment and altered Golgi morphology and reduced intraluminal pH, potentially explaining the glycosylation alterations.
AMERICAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Immunology
Christine Baksmeier, Pat Blundell, Julia Steckel, Verena Schultz, Quan Gu, Ana Da Silva Filipe, Alain Kohl, Chris Linnington, Dongli Lu, Anne Dell, Stuart Haslam, Jiabin Wang, Dan Czajkowsky, Norbert Goebels, Richard J. Pleass
Summary: IVIG is an established treatment for autoimmune conditions, but mechanisms of action remain unclear. This study used adapted Fc fragments to dissect IVIG effector mechanisms in an ex vivo model of demyelination. The protective effects of the adapted Fc fragments were dose-dependent and attributed to interference with complement-mediated damage to oligodendrocytes. Overall, findings suggest that recombinant biomimetics may be more effective than IVIG in controlling demyelination.
Review
Biotechnology & Applied Microbiology
Elizabeth Edwards, Maria Livanos, Anja Krueger, Anne Dell, Stuart M. Haslam, C. Mark Smales, Daniel G. Bracewell
Summary: Glycosylation plays a critical role in the quality and characteristics of therapeutic monoclonal antibodies in biologic manufacturing. Advances have been made in upstream processing, such as mammalian cell line engineering, to improve the predictability of glycosylation. Downstream processing strategies, including the use of novel technologies, are also being explored to control glycosylation. However, more research is needed to establish holistic and consistent manufacturing processes for antibody therapies.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Amber R. Peswani, Jaraspim Narkpuk, Anja Krueger, Daniel G. Bracewell, Porntippa Lekcharoensuk, Stuart M. Haslam, Anne Dell, Peera Jaru-Ampornpan, Colin Robinson
Summary: This study presents a new production workflow for an updated and cost-effective PCV2d vaccine candidate, showing high yields of purified PCV2d-based antigen from E. coli fermentation systems and a chimeric PCV2d-PCV3 capsid construct. The recombinant protein effectively induced capsid-specific and PCV2d-neutralizing antibodies in immunized animals, suggesting significant potential as a new vaccine candidate that can be easily manufactured at commercial scale.
FOOD AND BIOPRODUCTS PROCESSING
(2022)
Article
Public, Environmental & Occupational Health
Michael Axenhus, Sophia Schedin-Weiss, Bengt Winblad, Anders Wimo
Summary: The study found seasonal increased mortality within neurological diseases due to COVID-19, while mortality rates for infectious diseases, tumors, and cardiac diseases decreased. Changes in mortality trends for several common diseases were observed during the pandemic, indicating the diverse impact of COVID-19 on different health conditions.
SCANDINAVIAN JOURNAL OF PUBLIC HEALTH
(2022)
Article
Geriatrics & Gerontology
Michael Axenhus, Sophia Schedin-Weiss, Lars Tjernberg, Anders Wimo, Maria Eriksdotter, Gustaf Bucht, Bengt Winblad
Summary: This study evaluated the diagnosis trends of dementia in Sweden from 2015 to 2020 and found that the incidence of dementia diagnosis has been declining since 2015. The COVID-19 pandemic caused a further decrease in dementia diagnosis incidence in 2020, with a greater impact on women and the elderly.
Article
Microbiology
Lucy Duncombe, Laurence Howells, Anna Haughey, Andrew Taylor, Daryan Kaveh, Sevil Erdenlig Gurbilek, Anne Dell, Paul G. Hitchen, Stuart M. Haslam, Satadru Sekhar Mandal, N. Vijaya Ganesh, David R. Bundle, John McGiven
Summary: This study reveals the limitations of serological tests for brucellosis and demonstrates that adding chemical features to synthetic antigens can improve their specificity by blocking antibody binding to the tip of the OPS. This finding suggests the possibility of developing improved serological tests for brucellosis.
Article
Multidisciplinary Sciences
Gang Wu, Paola Grassi, David A. MacIntyre, Belen Gimeno Molina, Lynne Sykes, Samit Kundu, Cheng-Te Hsiao, Kay-Hooi Khoo, Phillip R. Bennett, Anne Dell, Stuart M. Haslam
Summary: This study used a comprehensive glycomic strategy to reveal the complex and abundant N-glycome in cervicovaginal fluid (CVF) of pregnant and non-pregnant women. The N-glycosylation profiles were found to differ in relation to pregnancy status, microbial composition, immune activation, and pregnancy outcome. Additionally, lower sialylation and higher fucosylation were observed in CVF from women who subsequently experienced preterm birth, indicating their potential roles in the mechanism of microbial driven preterm birth.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Zhengyuan Huang, Pei F. Lai, Alexander T. H. Cocker, Stuart M. Haslam, Anne Dell, Hugh J. M. Brady, Mark R. Johnson
Summary: Protein N-linked glycosylation is a diverse post-translational modification that stores biological information in a larger magnitude than other modifications. It impacts trophoblast functions and their interactions with decidual cells. Understanding N-glycosylation can improve approaches to predicting and diagnosing pregnancy complications related to placental dysfunction.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Meeting Abstract
Immunology
Belen Gimeno-Molina, Erna Bayar, Katherine Mountain, Ryan Love, Yun Lee, Ingrid Muller, Anne Dell, Stuart Haslam, Paola Grassi, Gang Wu, David MacIntyre, Phillip Bennett, Pascale Kropf, Lynne Sykes
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2023)
Meeting Abstract
Immunology
Katherine Mountain, David MacIntyre, Denise Chan, Alice Hyde, James Pasint-Magyar, Yun Lee, Richard Brown, Anna David, Anne Dell, Ten Feizi, Stuart Haslam, Yan Liu, Holly Lewis, Jane Norman, Sarah Stock, Tiong Teoh, Vasso Terzidou, Samit Kundu, Phillip Bennett, Lynne Sykes
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Chika Kikuchi, Aristotelis Antonopoulos, Shengyang Wang, Tadashi Maemura, Rositsa Karamanska, Chiara Lee, Andrew J. Thompson, Anne Dell, Yoshihiro Kawaoka, Stuart M. Haslam, James C. Paulson
Summary: The evolution of human H3N2 influenza viruses has led to a limited specificity of the hemagglutinin to a subset of glycan receptors, presenting challenges for virus recognition and vaccine production. By studying glyco-engineered cell lines, the authors demonstrate the importance of extended glycan receptors for the growth of recent H3N2 viruses and their relevance to vaccine production.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Yiran Xie, Fangqi Zhao, Nancy Freitag, Sophia Borowski, Yiru Wang, Charlotte Harms, Poh-Choo Pang, Juliette Desforges, Tianyu Wen, Edzard Schwedhelm, Manvendra Singh, Ralf Dechend, Anne Dell, Stuart M. Haslam, Gabriela Dveksler, Mariana G. Garcia, Sandra M. Blois
Summary: Placental abnormalities can lead to impaired fetal growth and poor pregnancy outcomes, such as preeclampsia (PE), with long-term consequences for both mother and offspring. Galectin-1 (gal-1), a glycan-binding protein expressed at the maternal-fetal interface, plays a critical role in maternal adaptation to pregnancy and placenta development. Deficiency of gal-1 during pregnancy is associated with the development of PE, but the specific contributions of maternal- and placental-derived gal-1 to the disease onset are not well understood. This study demonstrates that the lack of gal-1 increases the risk of PE development in a niche-specific manner, and this is accompanied by placental dysfunction primarily influenced by the absence of maternal-derived gal-1. Notably, differential placental glycosylation through Sda-capped N-glycans plays a dominant role in the invasive capacity of trophoblasts triggered by maternal-derived gal-1. These findings highlight the essential role of maternal-derived gal-1 in healthy placenta development and suggest that impairment of the gal-1 signaling pathway within the maternal niche may be a molecular cause of maternal cardiovascular maladaptation during pregnancy.
Meeting Abstract
Obstetrics & Gynecology
Katherine Mountain, David MacIntyre, Denise Chan, Alice Hyde, Yun Lee, Richard Brown, Anna David, Anne Dell, Ten Feizi, Stuart Haslam, Yan Liu, Holly Lewis, Jane Norman, Sarah Stock, T. G. Teoh, Vasso Terzidou, Samit Kundu, Phillip Bennett, Lynne Sykes
BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tong Lin, Lea S. van Husen, Yang Yu, Lars O. Tjernberg, Sophia Schedin-Weiss
Summary: The amyloid precursor protein (APP) is not only a precursor to the amyloid-beta peptide (A beta) in Alzheimer disease (AD), but also plays important roles in adhesion, signaling, neuronal development, and synaptic function. The lack of N-glycosylation at two sites on APP affects the trafficking and processing of APP, and may contribute to the development of AD.
