Article
Biochemistry & Molecular Biology
Christian Vahlensieck, Cora Sandra Thiel, Meret Mosimann, Timothy Bradley, Fabienne Caldana, Jennifer Polzer, Beatrice Astrid Lauber, Oliver Ullrich
Summary: Cellular effects of hypergravity, particularly its impact on gene expression in Jurkat T cells, were investigated in this study. The researchers observed a biphasic time course of gene expression response, with a transition point occurring at approximately 7 minutes. Upregulated genes shifted towards the center of the nuclei, while downregulated genes shifted towards the periphery. Interestingly, the gene expression pattern in response to hypergravity was distinct from that of other stress factors such as oxidative stress, heat shock, or inflammation. The researchers also highlighted the significant impact of routine centrifugation on the transcriptome of cells, recommending caution when using this method for RNA analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Jocelynda Salvador, M. Luisa Iruela-Arispe
Summary: Vascular cells are constantly influenced by physical forces associated with heart activities and vessel geometry, which regulate the transcriptional program and phenotype of these cells in different regions of the vascular tree. The nucleus plays a critical role as a mechanosensor, and maintaining nuclear integrity is essential for proper vascular function and stability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sergio Aguilera Suarez, Nadia Chandra Sekar, Ngan Nguyen, Austin Lai, Peter Thurgood, Ying Zhou, Scott Needham, Elena Pirogova, Khashayar Khoshmanesh, Sara Baratchi
Summary: The study introduces a motorized cam-driven system for cyclic stretch of aortic endothelial cells, which allows for generating customized spatiotemporal stretch profiles. Experiments demonstrate significant changes in cytoskeletal structure and morphology of cells following exposure to cyclic stretch.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Nadia Chandra Sekar, Sergio Aguilera Suarez, Ngan Nguyen, Austin Lai, Peter Thurgood, Ying Zhou, Chanly Chheang, Scott Needham, Elena Pirogova, Karlheinz Peter, Khashayar Khoshmanesh, Sara Baratchi
Summary: Endothelial cells, which line blood vessels, are affected by biophysical cues and play an important role in vascular health and disease. This study investigated the impact of vessel stiffness on endothelial cells and found that cells cultured on softer substrates showed different cellular alignment and morphology compared to cells on stiffer substrates.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Fabio Bertani, Dalila Di Francesco, Maria Dolores Corrado, Maria Talmon, Luigia Grazia Fresu, Francesca Boccafoschi
Summary: Cardiovascular diseases, including ischemic heart disease and stroke, are the leading cause of global mortality, with almost all sharing a common feature of endothelial dysfunction. Research suggests that ECs stimulated at low shear stress may be involved in paracrine induction of peripheral endothelial dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Austin Lai, Yung C. Chen, Charles D. Cox, Anthony Jaworowski, Karlheinz Peter, Sara Baratchi
Summary: This study used microfluidic technologies to investigate shear-induced sensitization of endothelial Piezo-1 to its selective agonist, Yoda-1, and found that the sensitization is transient and can be impaired by exposure to low and proatherogenic levels of shear stress. The process is independent of cell-cell adhesion and mediated by the PI3K-AKT signaling pathway. Additionally, shear stress was shown to increase the membrane density of Piezo-1 channels in endothelial cells.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
Tengteng Zhao, Sophia Parmisano, Zahra Soroureddin, Manjia Zhao, Lauren Yung, Patricia A. Thistlethwaite, Ayako Makino, Jason X. -J Yuan
Summary: TRPC6 and Piezo1 channels both contribute to the increase in intracellular calcium concentration in pulmonary arterial endothelial cells (PAECs), with TRPC6 being sensitive to diacylglycerol and Piezo1 being sensitive to membrane stretch.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Engineering, Biomedical
Lizebona August Ambattu, Callum Knight, Keng-hui Lin, Amy Gelmi, Leslie Y. Yeo
Summary: This study investigates the effects of short-duration mechanostimulation on endothelial junction stability and barrier capacity. The study found that the integrity of the endothelial barrier can not only recover but also enhance considerably after transient disruption, thanks to the role of calcium and cAMP signaling. Such enhancement in barrier capacity is useful for developing in vitro models that resemble in vivo conditions.
Review
Cell Biology
Hui Lin Zhou, Xi Zhuo Jiang, Yiannis Ventikos
Summary: This review summarizes the research on different blood fluid patterns and fluid shear stress in the circulation system, as well as the interactions between blood flow and endothelial cells. It highlights the role of the endothelial glycocalyx in maintaining endothelial health and discusses strategies for restoring damaged glycocalyx. This review provides a new perspective on the role of blood flow in regulating endothelial functionality.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Physiology
Chuyang Lin, Xiaolan Zheng, Sha Lin, Yue Zhang, Jinlin Wu, Yifei Li
Summary: Mechanical stress plays a critical role in the development, functional maturation, and pathogenesis of pulmonary tissues. Non-physiological mechanical forces can lead to various pulmonary diseases, with intracellular signaling being involved in this process.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Peripheral Vascular Disease
Ravi K. Adapala, Venkatesh Katari, Anantha K. Kanugula, Vahagn Ohanyan, Sailaja Paruchuri, Charles K. Thodeti
Summary: The study reveals that endothelial-specific deletion of TRPV4 helps preserve cardiac function, reduce cardiac fibrosis, and alleviate pathological cardiac hypertrophy by enhancing coronary angiogenesis.
Review
Cell & Tissue Engineering
Yan Huang, Jia-Yi Qian, Hong Cheng, Xiao-Ming Li
Summary: Studies have shown that shear stress can influence the differentiation of stem cells into endothelial cells, and further understanding of stem cells' response to shear stress is essential for the application of stem cells in cardiovascular implants in regenerative medicine.
WORLD JOURNAL OF STEM CELLS
(2021)
Review
Biology
Irina Ogneva
Summary: This review explores the effects of gravity changes on early embryos, stem cells, and terminally differentiated germ cells, and attempts to summarize the existing literature data and propose a possible unified mechanism for a single cell to receive and transmit gravity changes by altering the cellular cytoskeleton.
Article
Biochemistry & Molecular Biology
Michael W. Lai, Nathan Chow, Antonio Checco, Balvir Kunar, David Redmond, Shahin Rafii, Sina Y. Rabbany
Summary: This study investigates the effect of cyclic stretch on endothelial cells and identifies key responses and molecular regulatory networks. The findings have important implications for understanding vascular pathology and developing treatment modalities for vascular disease.
Article
Cardiac & Cardiovascular Systems
Dongwon Choi, Eunkyung Park, Roy P. Yu, Michael N. Cooper, Il-Taeg Cho, Joshua Choi, James Yu, Luping Zhao, Ji-Eun Irene Yum, Jin Suh Yu, Brandon Nakashima, Sunju Lee, Young Jin Seong, Wan Jiao, Chester J. Koh, Peter Baluk, Donald M. McDonald, Sindhu Saraswathy, Jong Y. Lee, Noo Li Jeon, Zhenqian Zhang, Alex S. Huang, Bin Zhou, Alex K. Wong, Young-Kwon Hong
Summary: This study identifies Piezo1 as an upstream mechanosensor that regulates lymphatic growth and sprouting through the mechanotransduction pathway. Activation of Piezo1 presents a novel therapeutic opportunity for preventing postsurgical lymphedema. The findings provide a molecular basis for Piezo1-associated lymphatic malformation in humans.
CIRCULATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Erika Terzuoli, Lucia Morbidelli, Ginevra Nannelli, Antonio Giachetti, Sandra Donnini, Marina Ziche
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Nutrition & Dietetics
Erika Terzuoli, Ginevra Nannelli, Antonio Giachetti, Lucia Morbidelli, Marina Ziche, Sandra Donnini
EUROPEAN JOURNAL OF NUTRITION
(2020)
Article
Multidisciplinary Sciences
Laura Micheli, Francesca Cialdai, Alessandra Pacini, Jacopo Junio Valerio Branca, Lucia Morbidelli, Valerio Ciccone, Elena Lucarini, Carla Ghelardini, Monica Monici, Lorenzo Di Cesare Mannelli
SCIENTIFIC REPORTS
(2019)
Review
Biochemistry & Molecular Biology
Amedeo Amedei, Lucia Morbidelli
Article
Cell Biology
Valerio Ciccone, Marco Zazzetta, Lucia Morbidelli
Article
Biochemistry & Molecular Biology
Francesca Cialdai, Alessandra Colciago, Desire Pantalone, Angela Maria Rizzo, Stefania Zava, Lucia Morbidelli, Fabio Celotti, Daniele Bani, Monica Monici
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Oncology
Erika Terzuoli, Cristiana Bellan, Sara Aversa, Valerio Ciccone, Lucia Morbidelli, Antonio Giachetti, Sandra Donnini, Marina Ziche
Article
Biochemistry & Molecular Biology
Valerio Ciccone, Arianna Filippelli, Andrea Angeli, Claudiu T. Supuran, Lucia Morbidelli
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Shirley Genah, Monica Monici, Lucia Morbidelli
Summary: Microgravity-induced bone loss is a significant health risk for space travelers as it can lead to irreversible changes and skeletal weakening. Various pharmacological and non-pharmacological countermeasures have been proposed, but each has limitations and potential serious side effects with prolonged use. More research is needed to develop innovative therapies for bone loss in microgravity conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Valerio Ciccone, Shirley Genah, Lucia Morbidelli
Summary: The vascular endothelium is composed of a single layer of cells that regulate blood flow homeostasis and tissue metabolic demands. Gasotransmitters such as nitric oxide and hydrogen sulfide, produced by endothelial cells, play a crucial role in maintaining vascular physiological functions. By regulating vascular function, H2S can enhance the biology and function of endothelial cells.
Article
Biochemistry & Molecular Biology
Shirley Genah, Francesca Cialdai, Valerio Ciccone, Elettra Sereni, Lucia Morbidelli, Monica Monici
Summary: The study demonstrates the effectiveness of PBMT in controlling fibroblast activation induced by inflammatory cytokines IL-1 beta and TNF-alpha, thereby suggesting a potential therapeutic approach for managing persistent inflammation.
Article
Oncology
Valerio Ciccone, Arianna Filippelli, Chiara Bacchella, Enrico Monzani, Lucia Morbidelli
Summary: This study investigates the antitumor effects of a novel nitric oxide (NO) donor, [Zn(PipNONO)Cl], on cancer cells and endothelial cells. The results demonstrate the potential therapeutic role of this NO donor in controlling tumor growth and metastasis by affecting epithelial-mesenchymal transition (EMT) and endothelial-mesenchymal transition (EndMT) processes.
Article
Biochemistry & Molecular Biology
Francesca Cialdai, Stefano Bacci, Virginia Zizi, Aleandro Norfini, Michele Balsamo, Valerio Ciccone, Lucia Morbidelli, Laura Calosi, Chiara Risaliti, Lore Vanhelden, Desiree Pantalone, Daniele Bani, Monica Monici
Summary: This study investigated the influence of low gravity on the healing of sutured human skin and vein wounds. The results showed that the tissue explants remained viable for an extended period of time and exhibited typical events associated with wound repair. This validates the suitability of the ex vivo organ culture system for wound healing studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Public, Environmental & Occupational Health
Arianna Filippelli, Valerio Ciccone, Stefano Loppi, Lucia Morbidelli
Summary: In organic agriculture, replacing synthetic pesticides with sweet chestnut wood distillate has shown success in yield and environmental benefits. Short-term exposure to the distillate is well tolerated by different cell lines, but prolonged exposure leads to concentration-dependent cytotoxic effects.
Review
Biochemistry & Molecular Biology
Ginevra Nannelli, Marina Ziche, Sandra Donnini, Lucia Morbidelli