Review
Chemistry, Analytical
Sheng Yan, Dan Yuan
Summary: The development of microfluidic technologies, particularly microflow cytometry with 3D focusing capabilities, has shown great promise in single-cell analysis, offering a wide range of applications in biology and medicine.
Article
Biotechnology & Applied Microbiology
Darryl Dieujuste, Yuhao Qiang, E. Du
Summary: This article presents the development and testing of a low-cost, portable electrical impedance-based microflow cytometer for single-cell analysis under controlled oxygen microenvironment. A case study on human red blood cells affected by sickle cell disease demonstrates the capability of the cytometry system in diagnosing diseases.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemical Research Methods
Ce Wang, Yuting Ma, Zhongxiang Chen, Yunliang Wu, Feifei Song, Jianping Qiu, Mengdie Shi, Xiaodong Wu
Summary: Microflow cytometry has gained popularity in recent years for its low-cost and disposable chips for complex cell analyses. This study demonstrates a sheathless microflow cytometer integrating acoustic waves for three-dimensional cell focusing, achieving high sensitivity and linearity. The system was successfully used for flow cytometry and immunologically labeled leukocytes differentiation.
Article
Chemistry, Analytical
Yushan Zhang, Andres Escobar, Tianyi Guo, Chang-Qing Xu
Summary: The eutrophication of aquatic ecosystems caused by urbanization has led to the production of hazardous bacterial blooms, such as cyanobacteria. Detecting and monitoring these blooms in real time is a challenge. This paper presents a microflow cytometry platform for label-free detection of cyanobacteria, which can provide early warning alerts for potential harmful blooms. The platform's detection limit can be as low as 5 cells/mL, significantly lower than the WHO's Alert Level 1 (2000 cells/mL). This improved detection limit may help authorities take timely actions to mitigate human risks.
Article
Chemistry, Multidisciplinary
Guantong Wang, Masaki Kudo, Kazuho Daicho, Sivasankaran Harish, Bin Xu, Cheng Shao, Yaerim Lee, Yuxuan Liao, Naoto Matsushima, Takashi Kodama, Fredrik Lundell, L. Daniel Soederberg, Tsuguyuki Saito, Junichiro Shiomi
Summary: This study demonstrates that nanocellulose materials can exhibit high thermal conductivity when their nanofibrils are highly aligned and bonded. The crystallinity of the filaments significantly influences their thermal conductivity.
Article
Chemistry, Analytical
Dezhi Tang, Lin Jiang, Wenlai Tang, Nan Xiang, Zhonghua Ni
Summary: This paper proposes a cost-effective portable microfluidic impedance cytometer (MIC) for broadband impedance analysis of cells using maximum length sequence (MLS) and viscoelastic focusing. Results show that MIC has the potential for rapid detection of circulating tumor cells.
Article
Pharmacology & Pharmacy
Roni Sverdlov Arzi, Asaf Kay, Yulia Raychman, Alejandro Sosnik
Summary: Nanoprecipitation and microfluidics technology are effective methods for producing pure drug nanoparticles and optimizing product performance. The use of specific microfluidic devices can lead to the production of rounded, optimized nanoparticles.
Article
Mechanics
M. Lopez, M. G. Cabezas, J. M. Montanero, M. A. Herrada
Summary: In this study, the stability of hydrodynamic focusing to produce microemulsions is investigated experimentally and numerically. It is found that the distance between the feeding capillary and the focusing orifice affects the liquid ejection stability. Confined selective withdrawal enhances the stability of the microdripping mode. Surfactants stabilize the meniscus and promote the transition from microdripping to jetting. The global stability analysis accurately predicts the critical flow rate ratio and the droplet emission frequency.
JOURNAL OF FLUID MECHANICS
(2022)
Article
Biology
Takuya Iida, Shota Hamatani, Yumiko Takagi, Kana Fujiwara, Mamoru Tamura, Shiho Tokonami
Summary: This study proposes a method for detecting attogram-level proteins using light-induced acceleration of antigen-antibody reactions in a microchannel platform. It achieves high sensitivity and ultrafast specific detection without any pretreatment procedures, demonstrating potential for the development of proteomics and innovative platforms for high-throughput bio-analyses.
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Analytical
Yang Zhou, Jiao Wang, Ting Liu, Man Wu, Yuwei Lan, Chunping Jia, Jianlong Zhao
Summary: Microfluidic impedance cytometry is a label-free, low-cost and portable solution for cell analysis. A miniaturized flow cytometer based on a 3D hydrodynamic focusing mechanism is designed and characterized. The use of an adaptive sheath effectively concentrates the sample and increases the signal-to-noise ratio of the particle impedance pulse. The system showed accurate representation of the particle impedance characteristic distribution and can be conveniently used to monitor cell status.
Article
Energy & Fuels
Can Ji, Zhigang Liu, Mingming Lv
Summary: This study experimentally investigates the detailed hydrodynamics in microchannels with in-line and staggered circular micro pin fin structures over a range of Reynolds numbers from 50 to 700. The results show that the onset of vortex shedding in micro pin fin arrays is delayed compared to their macro scale counterpart due to the confinement effect of channel walls. The flow behavior differs in the two arrangements, with the in-line arrangement featuring a microchannel-like main stream and a double vortex wake flow, while the staggered arrangement is characterized by a wavy main stream and a pair of accelerated flow streams.
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION
(2022)
Article
Engineering, Chemical
Yaser Kazemi, Arman Sadeghi, Abdullah Irankhah
Summary: Keeping reactants close to the reactive wall is crucial in microreactors where surface reactions are dominant. Single-phase hydrodynamic focusing is an effective technique, but has the drawback of high penetration of reactants into the sheath fluid. To overcome this, the concept of two-phase hydrodynamic focusing is introduced, using a highly viscous sheath fluid to create a barrier against reactant penetration.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Biochemical Research Methods
Wenhan Zhao, Xiaopeng Shang, Boran Zhang, Dan Yuan, Binh Thi Thanh Nguyen, Wenshuai Wu, Jing Bo Zhang, Niancai Peng, Ai Qun Liu, Fei Duan, Lip Ket Chin
Summary: This study systematically investigates the flow transition process in the hydrodynamic focusing mechanism, revealing the transition from the squeezed focusing state to the over-squeezed anti-focusing state. The recovery rate of cell analysis can be maintained by limiting the Reynolds number below 30. These findings provide new insights into microfluidic cytometric analyses with various applications in food safety, water monitoring, and healthcare sectors.
Article
Chemistry, Analytical
Diwakar M. Awate, Seth Holton, Katherine Meyer, Jaime J. Juarez
Summary: Flow focusing is an important technique for studying cellular samples, and this article presents low-cost millifluidic flow-focusing devices fabricated using a desktop 3D printer. Two printing strategies, monolithic SLA and hybrid molding, were used to create the devices. The experiments showed that the focusing widths achieved in these devices can be validated using a numerical model developed in ANSYS.
Article
Multidisciplinary Sciences
Alvin Modin, Matan Yah Ben Zion, Paul M. Chaikin
Summary: Researchers have developed a new active system that allows for the free rotation of micro-rotors while observing the interplay of rotation and translation. Using a non-tweezing circularly polarized beam, they were able to rotate hundreds of birefringent colloids and observe the orbiting of neighboring particles with different angular velocities. They also derived an analytical model to explain the observed dynamics.
NATURE COMMUNICATIONS
(2023)
Article
Physics, Fluids & Plasmas
Chun-Mao Wen, Che-Hsin Lin
Summary: This study developed a tube-based DBD plasma treatment for improving the performance of slippery coating layers on medical catheters. Results show that the contact angles of catheter tubes are significantly reduced after plasma treatment, and the hydrophilicity of the treated surface maintains 57% for more than 30 min. Catheters with plasma treatment exhibit lower kinetic friction force, indicating lower friction during medical insertion operations.
IEEE TRANSACTIONS ON PLASMA SCIENCE
(2021)
Article
Physics, Applied
Fu-Yao Yang, Fu-Sui Hung, Woon-Shing Yeung, Ruey-Jen Yang
Summary: This study proposes an optimization method for the design of planar arbitrary-geometry thermal cloaks using natural materials based on the bilayer theory in thermal-cloaking studies. The method aims to provide a practical design method for improved thermal-cloaking performance.
PHYSICAL REVIEW APPLIED
(2021)
Article
Chemistry, Analytical
Cheng-Tang Pan, Che-Hsin Lin, Ya-Kang Huang, Jason S. C. Jang, Hsuan-Kai Lin, Che-Nan Kuo, De-Yao Lin, Jacob C. Huang
Summary: Intervertebral fusion surgery is a major operation for spinal trauma, degeneration, and deformity correction. Most fusion cages have high stiffness, leading to stress concentration and shielding effects. Using a porous structure in fusion cages can reduce stiffness and improve strength for surrounding tissues. The study designed a porous gradient structure intervertebral cage and evaluated it through simulations and mechanical property testing.
Article
Chemistry, Analytical
Wei-Hao Huang, Van-Phung Mai, Ruo-Yin Wu, Ko-Li Yeh, Ruey-Jen Yang
Summary: This study proposes a sensor for detecting mercury and lead ions using graphene oxide and aptamer solution, with detection limits lower than the maximum limits set by the World Health Organization for drinking water.
Article
Chemistry, Multidisciplinary
Van-Phung Mai, Wei-Hao Huang, Ruey-Jen Yang
Summary: Thermo-osmotic energy conversion using waste heat is a method of harvesting sustainable energy while reducing environmental impacts. This study investigates the effect of surface charge and pH value on thermo-osmotic performance through numerical simulation, and finds that pH level has a significant impact on enhancing the thermo-osmotic conversion efficiency.
Review
Engineering, Biomedical
Po-Yuan Yeh, Jacob C. Huang, Jason S. C. Jang, Cheng-Tang Pan, Chung-Hwan Chen, Che-Hsin Lin
Summary: This paper reviews the recent developments of two newly developed intermetallic compounds as potential implantable biomaterials. The high strength, low Young's modulus, and excellent corrosion resistance make these compounds good candidates for bio-implantable materials. Additive manufacturing techniques provide an advantageous route for the preparation of these materials. A practical example confirms the feasibility of additive manufacturing for producing implantable intermetallic compound-based prostheses.
JOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING
(2022)
Article
Thermodynamics
Van-Phung Mai, Tsung-Yu Lee, Ruey-Jen Yang
Summary: This study fabricated different types of droplet-triboelectric nanogenerators and investigated the effect of electrowetting-assisted charge injection on their performance. The results showed that composite materials doped with silk powder and polytetrafluoroethylene particles significantly increased the output current, and further performance enhancement could be achieved through appropriate design of the dielectric layer and operating conditions.
Article
Engineering, Electrical & Electronic
Wei-Sin Kao, Yu-Wen Hung, Wen-Hsiang Liao, Yi-Chen Chou, Che-Hsin Lin
Summary: This article presents experimental validations of an industrial solid-state multisensor for simultaneous pH, electrical conductivity, and temperature detection of solutions. The sensor chip utilizes indium tin oxide as the sensing material and includes a thin-film deposited Ag/AgCl reference electrode. The experimental results show that the developed integrated sensor exhibits excellent performance in detecting pH, temperature, and solution conductivity.
IEEE TRANSACTIONS ON ELECTRON DEVICES
(2022)
Article
Engineering, Chemical
Van-Phung Mai, Wei-Hao Huang, Yong-Lin Chang, Ruey-Jen Yang
Summary: This study investigates the capillary-driven energy conversion performance of a 2D composite membrane consisting of a graphene oxide (GO) matrix and natural silk fibers. The results show that the composite structure of the GO@silk membrane provides enhanced physical robustness and improved ion selectivity. The experimental results demonstrate high voltage and current outputs of 0.22 V and 12 μA, respectively, indicating the feasibility of using composite membrane structures for environmental energy conversion on a larger scale.
JOURNAL OF MEMBRANE SCIENCE
(2023)
Article
Engineering, Electrical & Electronic
Wei-Sin Kao, Ling-Shan Yu, Che-Hsin Lin
Summary: A chip based on extended gate field effect transistor (EG-FET) with a precision temperature control system is developed for real-time and fluorescence-free loop-mediated isothermal amplification (LAMP) detection. The system detects the hydrogen ions released during nucleic acid amplification using a label-free, non-optical method, achieving stable and high detection performance. The system maintains a stable LAMP assay temperature and achieves a low limit of detection and short detection time.
IEEE SENSORS JOURNAL
(2023)
Article
Thermodynamics
Wei-Hao Huang, Chih-Chang Chang, Ruey-Jen Yang
Summary: This study conducted numerical simulations to investigate the thermoelectric response in nanofluidic channels with slip mechanism. The results showed that temperature-dependent ion electrophoretic mobility and thermoosmosis dominate the thermoelectric performance. This study provides valuable insights for developing efficient nanofluidic-based thermoelectric generators.
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
(2023)
Article
Chemistry, Physical
Po-Sung Chen, Pei-Hua Tsai, Tsung-Hsiung Li, Jason Shian-Ching Jang, Jacob Chih-Ching Huang, Che-Hsin Lin, Cheng-Tang Pan, Hsuan-Kai Lin
Summary: Ti-based metallic glasses have high potential for implant applications. A new biocompatible Ti-based bulk metallic glass composite was successfully fabricated through selective laser melting. The study demonstrates that the TiZrSiCoSnTa system shows promise for preparing biocompatible metallic glass composites into complicated graded foam shapes using additive manufacturing.
Article
Mechanics
Woon-Shing Yeung, Fu-Sui Hung, Van-Phung Mai, Ruey-Jen Yang
Summary: This paper presents a theoretical analysis of steady-state creeping flow in a composite porous domain with coupling heat transfer. The findings have important applications in thermal cloaking studies.
JOURNAL OF MECHANICS
(2022)
Review
Engineering, Environmental
Van-Phung Mai, Wei-Hao Huang, Ruey-Jen Yang
Summary: Blue energy, also known as salinity gradient energy, is considered a highly attractive solution for sustainable clean energy generation through ion transport via nanopassages like nanopores. This review discusses opportunities and challenges in ion transport through membranes of different dimensions, introduces strategies for enhancing ion transport and system performance, and explores mechanisms for improving ion transport through nanopores in the pursuit of harnessing blue energy efficiently in the future.
ACS ES&T ENGINEERING
(2021)
Article
Mechanics
Mahalakshmi Thangavelu, Nithyadevi Nagarajan, Hakan F. Oztop, Ruey-Jen Yang
Summary: Simulations were conducted to investigate magnetohydrodynamic convection of an Ag-water nanofluid in an inclined enclosure with a center heater, showing that heat transfer performance is influenced by the inclination angle and Hartmann number. Optimal heat transfer performance was achieved under specific conditions, and the heat transfer performance improves with an increasing volume fraction of nanoparticles.
JOURNAL OF MECHANICS
(2021)
