Article
Endocrinology & Metabolism
Xu Liu, Yang Liu, Hongzhong Liu, Haiyan Li, Jianhong Yang, Pei Hu, Xinhua Xiao, Dongyang Liu
Summary: Imigliptin and alogliptin, after 13 days of treatment, were shown to decrease glycemic levels by improving beta-cell function. Comparing OMM with HOMA or SUIT results, glucose stimulation may be more sensitive for detecting changes in beta-cell function.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Gisela Carrera-Alvarado, Fidel Toldra, Leticia Mora
Summary: This study successfully extracted peptides that can inhibit DPP-IV from different chicken blood hydrolysates prepared using different enzymes. AP and APP hydrolysates showed the best inhibitory effects, and several potential DPP-IV inhibitory peptides were identified. This study confirms the potential of peptides obtained from chicken blood hydrolysates to be used as DPP-IV inhibitors for the control and modulation of type 2 diabetes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Yoshinori Fujimura, Mototsugu Watanabe, Tomomi Morikawa-Ichinose, Konatsu Fujino, Mao Yamamoto, Seita Nishioka, Chihiro Inoue, Fumiyo Ogawa, Madoka Yonekura, Akari Nakasone, Motofumi Kumazoe, Hirofumi Tachibana
Summary: This study examined the DPP-IV inhibitory activity of leaf extracts from different Japanese green tea cultivars using liquid chromatography-mass spectrometry. It revealed differences in inhibitory rates among tea extracts and identified new compounds as DPP-IV inhibitors. The study also found that adding these compounds in combination can enhance the bioactivity of tea extract.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Endocrinology & Metabolism
Zhenhong Pan, Yan Yang, Jingjing Zhang
Summary: Combining DPP-IV inhibitors with insulin therapy in patients with type 2 diabetes mellitus significantly improves glycemic control without increasing the risk of hypoglycemia, adverse events, or serious adverse events compared to insulin treatment alone.
JOURNAL OF DIABETES
(2021)
Article
Biochemistry & Molecular Biology
Gisela Carrera-Alvarado, Fidel Toldra, Leticia Mora
Summary: Animal bones can be used as a sustainable pathway for the production of bioactive compounds. By pretreating bones with pepsin enzyme and then hydrolyzing them with Alcalase and Protana prime, researchers found that all three hydrolysates showed antioxidant and DPP-IV inhibitory activity, with the highest result obtained from the PAPP hydrolysate.
Article
Plant Sciences
Xinyuan Ye, Ling Xiong, Qifeng Fu, Binyou Wang, Yiwei Wang, Kailian Zhang, Jie Yang, Fahsai Kantawong, Warunee Kumsaiyai, Jie Zhou, Cai Lan, Jianming Wu, Jing Zeng
Summary: This study identified DPP-IV inhibitors within Gynura divaricata, suggesting that the extract of Gynura divaricata may be beneficial for the treatment of type 2 diabetes mellitus. It also provides a basis for further development and utilization of Gynura divaricata in herbal medicine.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Food Science & Technology
Friska Citra Agustia, Agnes Supriyadi, Agnes Murdiati, Retno Indrati
Summary: The DPP-IV inhibitory activity of jack bean sprouts was evaluated after digestion simulation and absorption. The results showed that the peptide fraction with a molecular weight of less than 1 kDa from 60-hour germinated jack bean hydrolysate (HG60) had the highest inhibitory activity. Most of the peptides with inhibitory activity were absorbed by the duodenum, accounting for 61.77% of the total. Two novel peptides, KAVGDPI and QGVVLRP, identified after absorption, contained crucial amino acids confirming their role as DPP-IV inhibitors.
FOOD SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Jing-Xian Wang, Xue-Jin Yang, Yan-Zhai Wang, Kai Yang, Huayou Chen, Yang-Chun Yong
Summary: A signal amplification system for electrochemical sensing was established using bio-nanohybrid cells based on bacterial self-assembly and biomineralization. This system showed high transmembrane electron transfer efficiency and achieved ultrasensitive detection of riboflavin.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Ritian Jin, Haowei Ren, Minhe Liao, Jiaqi Shang, Dangfeng Wang, Meng Li, Ning Liu
Summary: VLA was found to alleviate palmitic acid-induced cell death and ER stress by reducing DPP-IV activity and levels of ER stress marker proteins. These findings have implications for developing novel treatment approaches for liver diseases caused by ER stress.
Article
Chemistry, Analytical
Fangming Chen, Qian Guo, Qiaoyu Yang, Yao Meng, Mingrui Jiang, Jian Wang, De-Wen Zhang
Summary: Label-free electrochemical visualization of cancer cell apoptosis is achieved using a light-addressable electrochemical sensor. The study demonstrates that the AC photocurrent on apoptotic MCF-7 cells increases, indicating heterogeneity in apoptosis degrees. This new method provides potential applications in apoptosis-associated drug screening and efficacy evaluation.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Juan Wang, Yujia Xie, Yuanyuan Luan, Tingting Guo, Shanshan Xiao, Xingxing Zeng, Shaohui Zhang
Summary: The objective of this study was to isolate and identify intracellular bioactive peptides from mouse lymphocytes before and after lipopolysaccharide (LPS) stimulation. A total of 131 novel peptides were discovered, and their bioactivity and function were predicted. Two peptides with better inhibition activity were verified.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Endocrinology & Metabolism
Yutaka Seino, Kohei Kaku, Takashi Kadowaki, Taro Okamoto, Asako Sato, Masayoshi Shirakawa, Edward A. O'Neill, Samuel S. Engel, Keith D. Kaufman
Summary: In Japanese patients with type 2 diabetes, adding sitagliptin to ipragliflozin monotherapy provided significant improvement in glycemic control and was generally well tolerated.
DIABETES OBESITY & METABOLISM
(2021)
Article
Endocrinology & Metabolism
Kohei Kaku, Takashi Kadowaki, Yutaka Seino, Taro Okamoto, Masayoshi Shirakawa, Asako Sato, Edward A. O'Neill, Samuel S. Engel, Keith D. Kaufman
Summary: Adding ipragliflozin 50 mg once daily to sitagliptin 50 mg once daily monotherapy significantly improved glycemic control and was generally well tolerated in Japanese patients with type 2 diabetes.
DIABETES OBESITY & METABOLISM
(2021)
Article
Agriculture, Dairy & Animal Science
Arshida Ashraf, Priti Mudgil, Abdulrasheed Palakkott, Rabah Iratni, Chee-Yuen Gan, Sajid Maqsood, Mohammed Akli Ayoub
Summary: The molecular basis of the anti-diabetic properties of camel milk is still unknown, but recent studies suggest that camel whey proteins and their hydrolysates have an impact on in vivo activities. The results show inhibition of DPP-IV by these compounds and their positive effects on the activation of human insulin receptor and glucose uptake.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Nutrition & Dietetics
Yuchen Li, Gilda Aiello, Enrico Mario Alessandro Fassi, Giovanna Boschin, Martina Bartolomei, Carlotta Bollati, Gabriella Roda, Anna Arnoldi, Giovanni Grazioso, Carmen Lammi
Summary: This study focused on the extraction and hydrolysis of proteins from Chlorella pyrenoidosa (C. pyrenoidosa), showing potential hypotensive and hypoglycemic effects. Peptides derived from this process were found to inhibit ACE and DPP-IV activity effectively, with four peptides predicted to block both enzymes. The stability of these peptides during gastrointestinal digestion was also investigated.
Article
Electrochemistry
Jingwen Li, Yifei Li, Xingwei Zhai, Ya Cao, Jing Zhao, Yuguo Tang, Kun Han
ELECTROCHEMISTRY COMMUNICATIONS
(2020)
Review
Chemistry, Analytical
Xiaomeng Yu, Lingjun Sha, Langjian Dong, Ya Cao, Jing Zhao
Summary: Exosomes play a crucial role in intercellular transport and communication, and have the potential to serve as biomarkers for early detection and real-time monitoring of tumors. This review provides a summary of the recent advances in tumor exosome detection and their potential applications.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Bing Han, Cheng Fang, Lingjun Sha, Mohammed Jalalah, M. S. Al-Assiri, Farid A. Harraz, Ya Cao
Summary: A new fluorescent aptasensor using cascade strand displacement reaction was proposed for the detection of ochratoxin A, achieving an improved detection limit and short assay time. The method showed potential for use in food quality control in the future.
Article
Chemistry, Analytical
Bing Han, Langjian Dong, Lingling Li, Lingjun Sha, Ya Cao, Jing Zhao
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Chemistry, Analytical
Anzhi Sheng, Pei Wang, Jingyi Yang, Longfei Tang, Feng Chen, Juan Zhang
Summary: In this study, it was found that MXene can efficiently adsorb single-stranded DNA and be coupled with CRISPR-Cas12a for sensitive detection of LPS and bacteria. Through carefully designed aptamers, the trans-cleavage activity of CRISPR-Cas12a is activated, allowing for selective and sensitive quantification of targets in different samples. This method provides a new direction for the exploration of universal analytical methods based on MXene coupled with CRISPR-Cas12a.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Bing Han, Lingjun Sha, Xiaomeng Yu, Mei Yang, Ya Cao, Jing Zhao
Summary: The study introduced a novel method for simultaneous identification of dual therapeutic targets in breast cancer using in situ automatous DNA assembly reaction and electrochemical techniques. The method demonstrated high sensitivity and specificity in identifying co-existed therapeutic targets, even in serum samples, and could potentially provide valuable information for enhancing the efficiency of combined therapy in clinic. The use of quantum dots as electrochemical labeling allowed for the creation of increased electrochemical signaling to reveal the co-expression of the two targets.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Ying Wang, Langjian Dong, Jing Zhao, Mohammed Jalalah, M. S. Al-Assiri, Farid A. Harraz, Ya Cao
Summary: The developed dual-functional probe sensing method can specifically identify CD44 even in serum samples and breast cancer cells with broad detection range and high sensitivity. The method can monitor changes in CD44 expression, providing a new approach to study stem-like properties in breast cancer.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Tianxiang Xue, Longfei Tang, Xiquan Yue, Lili Niu, Jinhui Peng, Juan Zhang
Summary: A chemoselective ligation assisted DNA walker with input and output of double signals has been developed using oxime chemistry and alkyne-azide cycloaddition. This biosensor, designed for lipopolysaccharide (LPS) and 5-hydroxymethyl-2-furaldehyde (HMF) targets, can sensitively differentiate and analyze both targets with one-to-one mapping functionality. This work offers a new perspective on analyzing double targets based on chemoselective ligation assisted DNA walker.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Chemistry, Analytical
Pei Wang, Longfei Tang, Bohui Zhou, Liangfen Cheng, Robert Chunhua Zhao, Juan Zhang
Summary: Immune checkpoints are a series of molecules that regulate the degree of immune activation, including PD-1/PD-L1, CTLA-4, LAG-3, and others. Immune checkpoint therapy is considered to be a safer and more effective treatment for tumor immunotherapy compared to conventional therapy. The analysis of immune checkpoints is crucial for immunotherapy medication, treatment, and prognosis evaluation.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Liangfen Cheng, Fuhan Yang, Longfei Tang, Lelin Qian, Xu Chen, Feng Guan, Juan Zhang, Genxi Li
Summary: This study explores the use of cellular interface supported CRISPR/Cas trans-cleavage for electrochemical simultaneous detection of two types of sialic acids, Neu5Gc and Neu5Ac, which is important for evaluating tumor development. The method is simple, can quantitatively analyze sialic acids on cell surface, and can be used for the identification of bladder cancer cells.
Article
Chemistry, Analytical
Anzhi Sheng, Jingyi Yang, Liangfen Cheng, Juan Zhang
Summary: In this work, boronic ester-mediated dual recognition coupled with a CRISPR/Cas12a system has been proposed for highly specific and sensitive detection of lipopolysaccharide (LPS). The method utilizes boronic acid and LPS aptamer (LPSA) for simultaneous recognition, and CRISPR/Cas12a for signal amplification. The method has a linear detection range from 0.05 to 5000 ng/mL with a lowest detection limit of 44.86 pg/mL, and can be developed as a universal platform for the analytical detection of other carbohydrates.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yuxin Lin, Beibei Nie, Xinyu Qu, Minghui Wang, Jie Yang, Genxi Li
Summary: Covalent organic frameworks (COFs) have attracted much attention for their unique physical-chemical characteristics and potential applications in biosensing. In this study, spherical COFs with regular shape and good dispersion were synthesized, and a novel nanoprobe was developed by modifying Histostar on the COFs surface. The nanoprobe was then applied to fabricate an electrochemical biosensor for exosomes detection, achieving a low detection limit and wide linear detection range. This work offers a promising platform for ultrasensitive detection of exosomes.
Article
Nanoscience & Nanotechnology
Yingyan Zhao, Qi Liu, Yujia Qin, Yue Cao, Jing Zhao, Kai Zhang, Ya Cao
Summary: In this work, an electrochemical aptasensor was designed for the high-accuracy assay of AFP-L3 ratio. The aptasensor demonstrated the ability to accurately measure the AFP-L3 ratio in a linear range of 0.0008-40 ng mL-1, with satisfactory specificity and good recoveries in complex serum samples. This aptasensor may serve as a valuable tool for the measurement of AFP-L3 ratio and have great potential for early warning of hepatocellular carcinoma in clinical application.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Xiaomeng Yu, Ya Cao, Yingyan Zhao, Jianan Xia, Jie Yang, Yuanyuan Xu, Jing Zhao
Summary: This study presents a universal method for accurate and sensitive analysis of tumor-associated glycoprotein biomarkers. The method utilizes dual recognition of carbohydrates and proteins to achieve amplified electrochemical responses for glycoprotein analysis. The results show high accuracy, specificity, and versatility in complex serum samples, making this method a valuable tool for the analysis of glycoprotein biomarkers.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Xu Chen, Xianyong Wu, Ziyu He, Juan Zhang, Ya Cao, Dongsheng Mao, Chang Feng, Bo Tian, Guifang Chen
NEW JOURNAL OF CHEMISTRY
(2020)
