Article
Microbiology
Alexandra J. J. Lee, Georgia Doing, Samuel L. L. Neff, Taylor Reiter, Deborah A. A. Hogan, Casey S. S. Greene
Summary: This article studies the genomic heterogeneity of Pseudomonas aeruginosa strains, compares the expression patterns of core genes and accessory genes between PAO1 and PA14 strains, and finds differences in the expression patterns of some core genes among different strains.
Article
Microbiology
Ronika De, Marvin Whiteley, Rajeev K. Azad
Summary: This article introduces a gene co-expression network-based method for identifying pathogenicity-associated genes. By combining standard protocols with gene network methods, the ability to identify these genes in pathogens can be improved.
Article
Biochemistry & Molecular Biology
Qi Zhao, Yi-Feng Luo, Mi Tian, Yong-Long Xiao, Hou-Rong Cai, Hui Li
Summary: Pseudomonas aeruginosa induces macrophage senescence, which is regulated by the expression level of activating transcription factor 3 (ATF3). Manipulation of ATF3 expression affects macrophage senescence and phagocytic capacity, possibly through modulation of NF-kappa B translocation and inflammatory cytokine production.
MOLECULAR IMMUNOLOGY
(2021)
Review
Microbiology
Amber Grace, Rajnish Sahu, Donald R. R. Owen, Vida A. A. Dennis
Summary: Pseudomonas aeruginosa is a common pathogen that has been identified as a multi-drug resistant strain. PAO1 and PA14, two laboratory strains, have contributed significantly to our understanding of the bacterium. However, there are discrepancies between their behaviors and therapeutic susceptibilities observed in human infections. This review aims to compare the two strains and propose methods to improve their clinical relevance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Wenlu Zhang, Shasha Li, Bo Deng, Pengfei Wu, Yingbin Shui, Jiaming Zhang, Zhongjie Li
Summary: This study used RNA-sequencing to analyze sRNAs in antibiotic-resistant PAO1, finding a large number of differentially expressed sRNAs. Target gene prediction and pathway analysis revealed the potential roles of these differentially expressed sRNAs in regulating antibiotic resistance.
MICROBIAL PATHOGENESIS
(2022)
Article
Multidisciplinary Sciences
Fatemeh Saadati, Shahab Shahryari, Naeema Mohseni Sani, Davoud Farajzadeh, Hossein Shahbani Zahiri, Hojatollah Vali, Kambiz Akbari Noghabi
Summary: The study demonstrates that rhamnolipid biosurfactants extracted from Pseudomonas aeruginosa have good antibacterial and antibiofilm activity against MRSA ATCC6538 bacterial cells, making them promising candidates for therapeutic applications.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Hector Sanchez, George A. A. O'Toole, Brent Berwin
Summary: This study investigates the glycans used by P. aeruginosa PAO1 cells to bind to phagocytic cells and characterizes the suite of such molecules that can facilitate host cell binding by this microbe using a glycan array. The findings provide a better understanding of P. aeruginosa-glycan interactions and offer a valuable dataset for future studies in this area.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Analytical
Corinne Bure, Caroline Le Senechal, Luis Macias, Caroline Tokarski, Sebastien Vilain, Jennifer S. Brodbelt
Summary: Lipopolysaccharides (LPS) form the outermost layer of Gram-negative bacteria, playing a crucial role in bacterial infections. Understanding the structure of lipid A, which anchors LPS to the bacterial outer membrane, is important in elucidating LPS virulence and toxicity. Various successful strategies utilizing tandem mass spectrometry have been employed for the structural analysis of lipid A, revealing new acyl chain positional isomers in the lipid A profile of Pseudomonas aeruginosa PAO1.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Haizhu Jia, Yuan Chen, Yujing Chen, Ruihua Liu, Qionglin Zhang, Mark Bartlam
Summary: This study presents the crystal structure analysis of AEPT from Pseudomonas aeruginosa, revealing its catalytic mechanism in transamination as the first step in the AEP degradation pathway. The findings provide valuable insights for further functional and mechanistic studies of AEPT.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Hisashi Inoue, Teruyuki Tachibana, Tomohiro Bito, Jiro Arima
Summary: In this study, the acetylation reactions of acetylcholinesterase (AChE) were examined using different substrates. AChE was found to recognize ethyl acetate, diaminoalkanes, and aminoalcohols, leading to their acetylated products. The preference of AChE for acetyl donor and acceptor varied with pH, with ethyl acetate being preferred at alkaline pH and acetylcholine being preferred at acidic pH. Additionally, acetylated 6-amino-1-hexanol was decomposed by AChE.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zsofia Berkl, Ildiko Fekete-Kertesz, Kata Buda, Emese Vaszita, Eva Fenyvesi, Lajos Szente, Monika Molnar
Summary: Quorum sensing (QS) is a communication process in microorganisms that coordinates their activities through producing and detecting low-molecular-weight signal molecules. Cyclodextrins (CDs) can reduce signal communication and inhibit biofilm formation in pathogenic bacteria. The size, structure, and concentration of CDs are identified as key factors influencing biofilm formation. Alpha-CD and its random methylated and polymer derivatives demonstrate outstanding inhibitory efficiency.
Article
Microbiology
Carla Perez-Cruz, Ferran Brianso, Elisabeth Sonnleitner, Udo Blaesi, Elena Mercade
Summary: Studies on Pseudomonas aeruginosa PAO1 membrane vesicles (MVs) have shown that the externalization of RNA via MVs mainly occurs during the late exponential phase, suggesting the secretion of different types of MVs during growth processes.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Melanie Grosjean, Sophie Guenard, Caroline Giraud, Cedric Muller, Patrick Plesiat, Paulo Juarez
Summary: Pseudomonas aeruginosa is commonly used to study antibiotic resistance and pathogenesis, but its natural resistance to antibiotics and ability to produce virulence factors hinder its biotechnological potential. By reducing the genome of this bacterium, researchers developed hypovirulent and hypersusceptible recombinant DNA hosts, laying the foundation for further optimization for biotechnological applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Genetics & Heredity
Michele R. Castro, Graciela M. Dias, Tiago S. Salles, Nubia M. Cabral, Danielly C. O. Mariano, Hadassa L. Oliveira, Eliana S. F. W. Abdelhay, Renata Binato, Bianca C. Neves
Summary: The study revealed the important role of rhamnolipids in biofilm formation and cell motility of Pseudomonas aeruginosa, showing that lack of rhamnolipids can affect gene expression and lead to physiological differences. Furthermore, DNA microarrays can be used to investigate transcriptomic profiles of bacterial group behaviors.
Article
Multidisciplinary Sciences
Itai Sharon, Geoffrey A. McKay, Dao Nguyen, T. Martin Schmeing
Summary: In this study, a previously uncharacterized enzyme, cyanophycin dipeptide hydrolase (CphZ), was discovered and found to degrade dipeptides derived from cyanophycin. This expands the application of cyanophycin to a wider range of bacteria than previously appreciated.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biotechnology & Applied Microbiology
Jose A. Gutierrez-Barranquero, F. Jerry Reen, Maria L. Parages, Ronan McCarthy, Alan D. W. Dobson, Fergal O'Gara
MICROBIAL BIOTECHNOLOGY
(2019)
Review
Biotechnology & Applied Microbiology
F. Jerry Reen, Jose A. Gutierrez-Barranquero, Maria L. Parages, Fergal O'Gara
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Electrochemistry
Alyah Buzid, F. Jerry Reen, Fergal O'Gara, Gerard P. McGlacken, Jeremy D. Glennon, John H. T. Luong
ELECTROCHIMICA ACTA
(2018)
Article
Multidisciplinary Sciences
Si Tan, Jose A. Caparros-Martin, Vance B. Matthews, Henrietta Koch, Fergal O'Gara, Kevin D. Croft, Natalie C. Ward
SCIENTIFIC REPORTS
(2018)
Article
Microbiology
David F. Woods, Iwona M. Kozak, Stephanie Flynn, Fergal O'Gara
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Chemistry, Applied
Declan P. Gavin, Edel J. Murphy, Aoife M. Foley, Ignacio Abreu Castilla, F. Jerry Reen, David F. Woods, Stuart G. Collins, Fergal O'Gara, Anita R. Maguire
ADVANCED SYNTHESIS & CATALYSIS
(2019)
Review
Cardiac & Cardiovascular Systems
Adilah F. Ahmad, Girish Dwivedi, Fergal O'Gara, Jose Caparros-Martin, Natalie C. Ward
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2019)
Article
Microbiology
F. Jerry Reen, Jose A. Gutierrez-Barranquero, Ronan R. McCarthy, David F. Woods, Sara Scarciglia, Claire Adams, Kristian Fog Nielsen, Lone Gram, Fergal O'Gara
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Microbiology
Stephanie Flynn, F. Jerry Reen, Fergal O'Gara
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Medicine, General & Internal
Jose A. Caparros-Martin, Stephanie Flynn, F. Jerry Reen, David F. Woods, Patricia Agudelo-Romero, Sarath C. Ranganathan, Stephen M. Stick, Fergal O'Gara
Review
Infectious Diseases
David F. Woods, Stephanie Flynn, Jose A. Caparros-Martin, Stephen M. Stick, F. Jerry Reen, Fergal O'Gara
Summary: The imbalance in respiratory microbiota can lead to lung disease pathogenesis, with bile acids playing a key role in CF cases, associated with inflammation and restructuring of lung microbiota towards a pathogen-dominated state.
Article
Microbiology
Adilah F. Ahmad, Jose A. Caparros-Martin, Silvia Lee, Fergal O'Gara, Bu B. Yeap, Daniel J. Green, Mohammed Ballal, Natalie C. Ward, Girish Dwivedi
Summary: This observational cohort study found that there were no significant differences in gut bacterial diversity between obese patients before and after bariatric surgery compared to a healthy control group. However, there were differences in the abundance of certain bacterial groups between the two cohorts. Additionally, the study observed changes in the levels of specific short chain fatty acids and soluble CD14 and CD163 in obese patients before and after surgery compared to healthy controls.
Article
Microbiology
Stephanie Flynn, F. Jerry Reen, Jose A. Caparros-Martin, David F. Woods, Jorg Peplies, Sarath C. Ranganathan, Stephen M. Stick, Fergal O'Gara
Article
Microbiology
Jose A. Gutierrez-Barranquero, Maria L. Parages, Alan D. W. Dobson, F. Jerry Reen, Fergal O'Garaa
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)
Review
Microbiology
F. Jerry Reen, Gerard P. McGlacken, Fergal O'Gara
FEMS MICROBIOLOGY LETTERS
(2018)