Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies

Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae

(2014) Mathias Jørgensen et al.   Microbial Cell Factories

Nanobody-based products as research and diagnostic tools

(2014) Thomas De Meyer et al.   TRENDS IN BIOTECHNOLOGY

Rapid optical imaging of human breast tumour xenografts using anti-HER2 VHHs site-directly conjugated to IRDye 800CW for image-guided surgery

(2013) Marta Kijanka et al.   EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING

Broad host range vectors for expression of proteins with (Twin-) Strep-tag, His-tag and engineered, export optimized yellow fluorescent protein

(2013) Thorben Dammeyer et al.   Microbial Cell Factories

Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

(2013) Susan Schlegel et al.   Microbial Cell Factories

High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli

(2013) Kristof Zarschler et al.   Microbial Cell Factories

The effect of uniform capture molecule orientation on biosensor sensitivity: Dependence on analyte properties

(2012) Anke K. Trilling et al.   BIOSENSORS & BIOELECTRONICS

High-yield export of a native heterologous protein to the periplasm by the tat translocation pathway in Escherichia coli

(2012) Cristina F.R.O. Matos et al.   BIOTECHNOLOGY AND BIOENGINEERING

Methodologies for the isolation of alternative binders with improved clinical potentiality over conventional antibodies

(2012) Ario de Marco   CRITICAL REVIEWS IN BIOTECHNOLOGY

Recent contributions in the field of the recombinant expression of disulfide bonded protein in bacteria

(2012) Ario de Marco   Microbial Cell Factories

Enzymatic Single-Chain Antibody Tagging

(2011) H.T. Ta et al.   CIRCULATION RESEARCH

Immuno-imaging using nanobodies

(2011) Ilse Vaneycken et al.   CURRENT OPINION IN BIOTECHNOLOGY

The concurrent use of N- and C-terminal antibodies anti-nucleophosmin 1 in immunofluorescence experiments allows for precise assessment of its subcellular localisation in acute myeloid leukaemia patients

(2011) A M Gruszka et al.   LEUKEMIA

Antibody purification-independent microarrays (PIM) by direct bacteria spotting on TiO2-treated slides

(2011) Marzia L. De Marni et al.   METHODS

Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli

(2011) Van Nguyen et al.   Microbial Cell Factories

High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation

(2011) Dirk Ponsel et al.   MOLECULES

Beyond natural antibodies: the power of in vitro display technologies

(2011) Andrew R M Bradbury et al.   NATURE BIOTECHNOLOGY

Improved quantitative and qualitative production of single-domain intrabodies mediated by the co-expression of Erv1p sulfhydryl oxidase

(2011) Gianluca Veggiani et al.   PROTEIN EXPRESSION AND PURIFICATION

Superfolder GFP Is Fluorescent in Oxidizing Environments When Targeted via the Sec Translocon

(2011) Deborah E. Aronson et al.   TRAFFIC

A Major Role of p95/611-CTF, a Carboxy-Terminal Fragment of HER2, in the Down-modulation of the Estrogen Receptor in HER2-Positive Breast Cancers

(2010) J. L. Parra-Palau et al.   CANCER RESEARCH

Comprehensive engineering of Escherichia coli for enhanced expression of IgG antibodies

(2010) Tomohiro Makino et al.   METABOLIC ENGINEERING

Screening optimized protein purification protocols by coupling small-scale expression and mini-size exclusion chromatography

(2010) Elisa Sala et al.   PROTEIN EXPRESSION AND PURIFICATION

A multi-Fc-species system for recombinant antibody production

(2009) Sandrine Moutel et al.   BMC BIOTECHNOLOGY

Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli

(2009) Ario de Marco   Microbial Cell Factories

Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library

(2009) Ana Monegal et al.   PROTEIN ENGINEERING DESIGN & SELECTION

E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display

(2008) Yariv Mazor et al.   Nature Protocols