Recombinant polypeptide production in E. coli: towards a rational approach to improve the yields of functional proteins

Packaging protein drugs as bacterial inclusion bodies for therapeutic applications

(2013) Antonio Villaverde et al.   Microbial Cell Factories

A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli

(2013) Simone Balzer et al.   Microbial Cell Factories

High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli

(2013) Hervé Nozach et al.   Microbial Cell Factories

Control of total GFP expression by alterations to the 3′ region nucleotide sequence

(2013) Sang Lee et al.   Microbial Cell Factories

Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures

(2013) Kaisa Ukkonen et al.   Microbial Cell Factories

Bacterial inclusion bodies as potential synthetic devices for pathogen recognition and a therapeutic substance release

(2013) Klaudia Talafová et al.   Microbial Cell Factories

A single mutation in the core domain of the lac repressor reduces leakiness

(2013) Pietro Gatti-Lafranconi et al.   Microbial Cell Factories

Screening and identification of genetic loci involved in producing more/denser inclusion bodies in Escherichia coli

(2013) Neeraj Pandey et al.   Microbial Cell Factories

Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

(2013) Susan Schlegel et al.   Microbial Cell Factories

Why and how protein aggregation has to be studied in vivo

(2013) Diletta Ami et al.   Microbial Cell Factories

Combinatorial Mutagenesis and Selection of Improved Signal Sequences and Their Application for High-Level Production of Translocated Heterologous Proteins in Escherichia coli

(2012) Tonje Marita Bjerkan Heggeset et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Optimizing Membrane Protein Overexpression in the Escherichia coli strain Lemo21(DE3)

(2012) Susan Schlegel et al.   JOURNAL OF MOLECULAR BIOLOGY

Multi-copy genes that enhance the yield of mammalian G protein-coupled receptors in Escherichia coli

(2012) Georgios Skretas et al.   METABOLIC ENGINEERING

Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette

(2012) Friederike Zwick et al.   Microbial Cell Factories

Recent contributions in the field of the recombinant expression of disulfide bonded protein in bacteria

(2012) Ario de Marco   Microbial Cell Factories

Effects of a recombinant gene expression on ColE1-like plasmid segregation in Escherichia coli

(2011) Mladen Popov et al.   BMC BIOTECHNOLOGY

High-throughput protein expression screening and purification in Escherichia coli

(2011) Renaud Vincentelli et al.   METHODS

Enhanced expression of membrane proteins in E. coli with a PBAD promoter mutant: synergies with chaperone pathway engineering strategies

(2011) Brent L Nannenga et al.   Microbial Cell Factories

Active protein aggregates induced by terminally attached self-assembling peptide ELK16 in Escherichia coli

(2011) Wei Wu et al.   Microbial Cell Factories

Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli

(2011) Van Nguyen et al.   Microbial Cell Factories

Strain engineering for improved expression of recombinant proteins in bacteria

(2011) Tomohiro Makino et al.   Microbial Cell Factories

Too many roads not taken

(2011) Aled M. Edwards et al.   NATURE

Reprogramming chaperone pathways to improve membrane protein expression in Escherichia coli

(2011) Brent L. Nannenga et al.   PROTEIN SCIENCE

Recombinant protein quality evaluation: proposal for a minimal information standard

(2011) Ashley M. Buckle et al.   Standards in Genomic Sciences

Comprehensive engineering of Escherichia coli for enhanced expression of IgG antibodies

(2010) Tomohiro Makino et al.   METABOLIC ENGINEERING

Tunable geometry of bacterial inclusion bodies as substrate materials for tissue engineering

(2010) Elena García-Fruitós et al.   NANOTECHNOLOGY

HSP90 at the hub of protein homeostasis: emerging mechanistic insights

(2010) Mikko Taipale et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli

(2009) Ario de Marco   Microbial Cell Factories

Conditional gene silencing of multiple genes with antisense RNAs and generation of a mutator strain of Escherichia coli

(2009) Nobutaka Nakashima et al.   NUCLEIC ACIDS RESEARCH

Directed evolution of the transcription factor XylS for development of improved expression systems

(2009) Trond Erik Vee Aune et al.   Microbial Biotechnology

High-Throughput, Combinatorial Engineering of Initial Codons for Tunable Expression of Recombinant Proteins

(2008) Jin-Ho Ahn et al.   JOURNAL OF PROTEOME RESEARCH

Tuning Escherichia coli for membrane protein overexpression

(2008) S. Wagner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA