Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid hormone receptor 1

(2010) Kerstin Michalke et al.   ANALYTICAL BIOCHEMISTRY

Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: A case study of the human endothelin A receptor

(2010) Friederike Junge et al.   JOURNAL OF STRUCTURAL BIOLOGY

A purified C-terminally truncated human adenosine A2A receptor construct is functionally stable and degradation resistant

(2010) Shweta Singh et al.   PROTEIN EXPRESSION AND PURIFICATION

Structures of the CXCR4 Chemokine GPCR with Small-Molecule and Cyclic Peptide Antagonists

(2010) B. Wu et al.   SCIENCE

Structure of the Human Dopamine D3 Receptor in Complex with a D2/D3 Selective Antagonist

(2010) E. Y. T. Chien et al.   SCIENCE

Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris

(2009) Takami Yurugi-Kobayashi et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

From purified GPCRs to drug discovery: the promise of protein-based methodologies

(2009) Fatima Alkhalfioui et al.   CURRENT OPINION IN PHARMACOLOGY

Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies

(2008) Kerstin Michalke et al.   ANALYTICAL BIOCHEMISTRY

Identification of functional bradykinin B2 receptors endogenously expressed in HEK293 cells

(2008) Inga I. Kramarenko et al.   BIOCHEMICAL PHARMACOLOGY

Using EGFP fusions to monitor the functional expression of GPCRs in the Drosophila Schneider 2 cells

(2008) Karl Brillet et al.   CYTOTECHNOLOGY

Crystal Structure of Squid Rhodopsin with Intracellularly Extended Cytoplasmic Region

(2008) Tatsuro Shimamura et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Functional Analysis of Kv1.2 and Paddle Chimera Kv Channels in Planar Lipid Bilayers

(2008) Xiao Tao et al.   JOURNAL OF MOLECULAR BIOLOGY

GPCR Proteomics: Mass Spectrometric and Functional Analysis of Histamine H1Receptor after Baculovirus-Driven andin VitroCell Free Expression

(2008) Kamonchanok Sansuk et al.   JOURNAL OF PROTEOME RESEARCH

Large-scale functional expression of WT and truncated human adenosine A2A receptor in Pichia pastoris bioreactor cultures

(2008) Shweta Singh et al.   Microbial Cell Factories

Structure of a β1-adrenergic G-protein-coupled receptor

(2008) Tony Warne et al.   NATURE

Crystal structure of squid rhodopsin

(2008) Midori Murakami et al.   NATURE

Crystal structure of the ligand-free G-protein-coupled receptor opsin

(2008) Jung Hee Park et al.   NATURE

Efficient cell-free production of olfactory receptors: Detergent optimization, structure, and ligand binding analyses

(2008) L. Kaiser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A novel, generic and effective method for the rapid purification of G protein-coupled receptors

(2008) Thierry Magnin et al.   PROTEIN EXPRESSION AND PURIFICATION

The 2.6 Angstrom Crystal Structure of a Human A2A Adenosine Receptor Bound to an Antagonist

(2008) V.-P. Jaakola et al.   SCIENCE