Article
Chemistry, Physical
Maksim Gorbachevskii, Anna Stavitskaya, Andrei A. Novikov, Rawil F. Fakhrullin, Elvira Rozhina, Ekaterina A. Naumenko, Vladimir A. Vinokurov
Summary: Fluorescent gold nanoclusters (AuNCs) stabilized with 11-mercaptoundecanoic acid and supported on halloysite nanotubes modified with aminosilane showed good uptake by A549 human cells. At concentrations of 25-50 μg/mL, the material did not have a pronounced toxic effect but led to massive cell death via apoptosis at 100 μg/mL. This concentration-dependent toxicity mechanism allows for potential applications in bioimaging and cancer therapy.
APPLIED CLAY SCIENCE
(2021)
Article
Chemistry, Physical
Yingzi Zhang, Xu Lingling, Zhuo Liu, Xi Cui, Zhuo Xiang, Jinyu Bai, Dongjie Jiang, Jiangtao Xue, Chan Wang, Youxi Lin, Zhe Li, Yizhu Shan, Yuan Yang, Lin Bo, Zhou Li, Xiaozhong Zhou
Summary: The use of a biomechanical-energy-driven shape memory piezoelectric nanogenerator can promote osteogenic differentiation. Pulsed direct current effectively enhances the proliferation and calcium ion increase of preosteoblasts, and improves ALP activity. Ultimately, this method can promote calcium deposition, extracellular matrix mineralization, and osteogenesis.
Article
Biochemistry & Molecular Biology
Felix Hoeren, Zeliha Goermez, Manfred Richter, Kerstin Troidl
Summary: This study developed a deep learning-supported image processing tool, Deetect, for the analysis of cell deposits on oxygenator membranes. Compared with traditional segmentation methods, Deetect showed better performance in clustered areas and provided a more objective approach for regions with difficult visual distinction.
Article
Chemistry, Physical
Olympia Geladari, Martin Eberle, Andre Maier, Florian Fetzer, Thomas Chasse, Alfred J. Meixner, Marcus Scheele, Andreas Schnepf, Kai Braun
Summary: Flexible electronics manufacturing technologies are crucial and highly favored for future integrated photonic and electronic devices. A direct laser induced writing (DIW) technique has been developed for adaptable electronics, enabling electrical gold wiring of 2D van-der-Waals materials and fabrication of photo switches and field effect transistors. This technique utilizes light-sensitive metalloid Au-32-nanoclusters as ink, allowing for low-power cw-laser exposure and easy removal of unexposed ink.
Article
Engineering, Biomedical
Alula R. Yohannes, Christopher Y. Jung, Katherine Shea, Wai T. Wong, Alexander Beylin, Ethan D. Cohen
Summary: The study found that the morphological responses of retinal microglia to electrical overstimulation injury depended on their spatial location relative to the electrode lumen, leading to three different morphological responses. These responses included severely injured microglia becoming immotile ball-like processes, surviving microglia rapidly extending filopodia towards the damage zone, and a fading of fluorescence in deeper outer microglia under the electrode.
JOURNAL OF NEURAL ENGINEERING
(2021)
Article
Materials Science, Multidisciplinary
Aditya Burla, Mudrika Khandelwal, Mayur Vaidya
Summary: This study demonstrates the antibacterial properties of CoCrCuNi and CoCrCuFeNi complex concentrated alloys (CCAs) against Escherichia coli and investigates the effect of copper addition on the antibacterial properties. The results show that the Cu-containing CCAs exhibit superior antibacterial efficiency and apatite formation on the alloy surface.
MATERIALS TODAY COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Hyun Jeong Jeon, Ara Jung, Hee Jin Kim, Jeong San Seo, Jun Young Kim, Moon Seop Yum, Bomi Gweon, Youbong Lim
Summary: This study proposes a vacuum plasma device for surface treatment of dental implants, showing significant improvements in protein adsorption, cell adhesion, and proliferation efficiency through plasma treatment. The device has the potential to enhance osseointegration efficiency.
APPLIED SCIENCES-BASEL
(2022)
Article
Cell Biology
Anne Valat, Laure Fourel, Adria Sales, Paul Machillot, Anne-Pascale Bouin, Carole Fournier, Lauriane Bosc, Melanie Arboleas, Ingrid Bourrin-Reynard, Amy Wagoner J. Johnson, Franz Bruckert, Corinne Albiges-Rizo, Catherine Picart
Summary: This study investigated how BMP-2 induces osteogenic programming and changes in adhesive machinery in C2C12 myoblasts. The results showed that the switch of integrins and cadherins, as well as the expression of specific transcription factors, are associated with muscle to bone transdifferentiation upon BMP-2 stimulation. The use of biomimetic films revealed the distinct roles of integrins and cadherins in driving osteogenic programming.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Polymer Science
Raffael Rathner, Claudia Leimhofer, Wolfgang Roland, Alexander Hammer, Bernhard Loew-Baselli, Georg Steinbichler, Sabine Hild
Summary: Co-extrusion is a commonly used method for producing polymer multilayer products. The adhesion between layers is crucial for the overall performance of the structure. This study investigated the influence of different processing conditions on layer adhesion and found that adjusting the shear load can significantly improve the adhesion. These findings open up new opportunities and potentials for multi-layer products.
Article
Microbiology
Sarah M. Yannarell, Dusan Velickovic, Christopher R. Anderton, Elizabeth A. Shank
Summary: The study developed a method to analyze the molecular localization within sagittal thin sections of B. subtilis biofilms using high-resolution mass spectrometry imaging. Correlations between specific molecules and phenotypic reporters suggested the roles of surfactin and bacillibactin in gene expression and iron scavenging in the biofilm. This technique provides a platform to generate hypotheses about molecule-cell relationships in biofilm-forming bacteria.
Article
Cell Biology
Susanne Staehlke, Meike Bielfeldt, Julius Zimmermann, Martina Gruening, Ingo Barke, Thomas Freitag, Sylvia Speller, Ursula Van Rienen, Barbara Nebe
Summary: Electrical stimulation plays a positive role in cell activation in bone tissue regeneration, promoting the attachment and initial adhesion process of osteoblasts, as well as enhancing intracellular Ca2+ levels. This research provides new insights into the mechanisms of electrical stimulation in bone tissue regeneration.
Article
Chemistry, Analytical
Xinyi Hua, Zhifang Wang, Ziqi Wang, Linlin Chen, Zaichun Zhou, Junlin Ouyang, Keqin Deng, Xiumei Yang, Haowen Huang
Summary: An innovative biosensing assay was developed for simplified, cost-effective, and sensitive detection. By treating target proteins with iron porphyrin in situ, a stable protein complex with peroxidase activity was formed, providing visible signals comparable to ELISA. This immunoassay eliminates the need for expensive cross-linking processes, making it practical for clinical diagnosis, food safety, and environmental monitoring.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Takuto Fujii, Takahiro Shimizu, Mizuki Katoh, Shushi Nagamori, Keiichi Koizumi, Junya Fukuoka, Yoshiaki Tabuchi, Akira Sawaguchi, Tomoyuki Okumura, Kazuto Shibuya, Tsutomu Fujii, Hiroshi Takeshima, Hideki Sakai
Summary: The study reveals that Na+,K+-ATPase alpha 3-isoform is translocated from intracellular vesicles to the plasma membrane of detached cancer cells, contributing to their survival. Focal-adhesion-kinase-dependent Ca2+ response induces alpha 3NaK translocation, associated with activation of AMP-activated protein kinase.
Review
Oncology
Zahra Nozhat, Mina S. Khalaji, Mehdi Hedayati, Sima Kheradmand Kia
Summary: The choice of cell viability assays depends on factors such as cost, speed, and complexity. Each method has its advantages and disadvantages, and using at least two methods simultaneously can provide a more comprehensive evaluation of the effects of different agents on different cell types.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Mohamad Allaw, Maria Manconi, Pierluigi Caboni, Gianluigi Bacchetta, Elvira Escribano-Ferrer, Jose Esteban Peris, Amparo Nacher, Octavio Diez-Sales, Maria Letizia Manca
Summary: Pistacia lentiscus L. can be used to produce various products such as essential oil, mastic gum, and fixed oil. Incorporating lentisk oil into liposomes can enhance its penetration ability in the skin and potentially treat skin lesions.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Materials Science, Ceramics
Jose D. Avila, Amit Bandyopadhyay
Summary: Directed energy deposition (DED) was utilized to produce niobium carbide (NbC)-reinforced Ti6Al4V (Ti64) metal-matrix-composite (MMC) structures, with the objective of enhancing Ti64's wear and oxidation resistance. Results indicated that the addition of NbC particles aided in increasing Ti64's resistance to plastic shear and led to a reduction in specific wear rate.
INTERNATIONAL JOURNAL OF APPLIED CERAMIC TECHNOLOGY
(2022)
Article
Engineering, Biomedical
Sushant Ciliveri, Amit Bandyopadhyay
Summary: This study evaluated the mechanical properties of additively manufactured porous Ti6Al4V structures under compression and shear loading. The results showed that shear behavior is more sensitive to variations in cell size, while elastic modulus is more sensitive to changes in strut size. The study also found that a pore size of 670 μm demonstrated the highest osteoblast cell viability and bacterial cell viability was higher in porous structures compared to dense Ti.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Amit Bandyopadhyay, Susmita Bose, Roger Narayan
Summary: This article reviews the impact of technological development on the manufacturing of biomedical devices and discusses the progress and regulatory issues of 3D printing biomaterials and medical devices.
Article
Nanoscience & Nanotechnology
David Svetlizky, Baolong Zheng, Alexandra Vyatskikh, Mitun Das, Susmita Bose, Amit Bandyopadhyay, Julie M. Schoenung, Enrique J. Lavernia, Noam Eliaz
Summary: Directed energy deposition (DED) has become an important branch of additive manufacturing (AM) and is widely used in the design and fabrication of various materials. Successful DED operation requires a good understanding of critical phenomena such as laser-material interactions, alloy solidification, welding metallurgy, and microstructure-mechanical properties relations. This comprehensive review focuses on materials design via DED, including a survey of different compositions and highlights the challenges and opportunities in this area.
MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING
(2022)
Article
Materials Science, Coatings & Films
Arjak Bhattacharjee, Amit Bandyopadhyay, Susmita Bose
Summary: Titanium alloys have excellent fatigue and corrosion resistance, high strength to weight ratio, and poor osseointegration ability, which can lead to implant loosening. Hydroxyapatite coating on titanium surfaces is commonly used to enhance osseointegration. The addition of zinc ions and fluoride improves the biological properties of hydroxyapatite. Parametric optimizations are used to fabricate zinc and fluoride doped hydroxyapatite-coated titanium, resulting in coatings with optimized thicknesses. The doped coatings show higher antibacterial efficacy and osteoblast viability compared to pure hydroxyapatite-coated samples. These coatings have potential applications in load-bearing implants for revision surgeries and immune-compromised patients.
SURFACE & COATINGS TECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Kellen D. Traxel, Amit Bandyopadhyay
Summary: The desire for higher application temperatures and complex geometries for metallic materials has driven significant development in additive manufacturing (AM) of metal-ceramic composites. However, there is limited understanding of the process-microstructure-properties relationships for these materials and processing strategies. In this study, the processing window and high-temperature oxidation performance of an in situ reactive, oxidation-resistant titanium metal-matrix composite reinforced with boron nitride (BN) and boron carbide (B4C) were investigated via selective laser melting (SLM) to understand the effects of processing parameters on the in situ reactive characteristics and their influence on build reliability and high-temperature oxidation performance. Process optimization resulted in composites with high relative density and improved high-temperature oxidation resistance compared to the base material.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2022)
Review
Engineering, Manufacturing
Amit Bandyopadhyay, Yanning Zhang, Bonny Onuike
Summary: Bimetallic structures are in high demand for current industrial applications due to their unique properties. However, welding techniques face challenges in joining dissimilar metals, leading to heat-affected zone management and brittle intermetallic phase issues. Metal additive manufacturing (AM) has emerged as a promising solution for processing bimetallic structures. This review provides insights into various processing strategies, challenges, and future directions for the implementation of AM technologies in bimetallic structures.
VIRTUAL AND PHYSICAL PROTOTYPING
(2022)
Article
Nanoscience & Nanotechnology
Aniruddha Pal, Rathina Vel, Sk Hasanur Rahaman, Somoshree Sengupta, Subhadip Bodhak
Summary: The study focuses on the synthesis and characterization of a sugar-glass nanoparticle (SGnP) based protein delivery system for tissue engineering applications. Various experiments confirmed the successful preparation of SGnP and its efficient encapsulation and release of proteins. SGnP demonstrated excellent stability and protective effects on encapsulated proteins.
Article
Engineering, Manufacturing
Yanning Zhang, Cory Groden, E. Nyberg, A. Bandyopadhyay
Summary: Bimetallic structures of Ti6Al4V-W7Ni3Fe were fabricated using directed energy deposition-based additive manufacturing. The research demonstrates the ability to control the mechanical and thermal performance of the bimetallic structures, with higher thermal conductivity and variable failure strain compared to pure Ti6Al4V.
VIRTUAL AND PHYSICAL PROTOTYPING
(2023)
Article
Nanoscience & Nanotechnology
Aniruddha Pal, Puja Das Karmakar, Rathina Vel, Subhadip Bodhak
Summary: This study focuses on the synthesis and characterization of a bioactive glass nanoparticle-reinforced poly(ethylene glycol) (PEG) and poly(N-vinylcarbazole) (pNVC) based minimally invasive composite injectable hydrogel suitable for bone regeneration. The nanocomposite hydrogel showed good gelling and injectability properties, and promoted bone cell proliferation and osteogenesis in vitro. Therefore, it has the potential to be used as a grafting material in orthopedic reconstructive surgeries.
ACS APPLIED BIO MATERIALS
(2023)
Review
Materials Science, Multidisciplinary
Amit Bandyopadhyay, Indranath Mitra, Stuart B. Goodman, Mukesh Kumar, Susmita Bose
Summary: The increasing demand for joint replacement surgeries, musculoskeletal repairs, and orthodontics worldwide drives the need for evolving technologies in healthcare. Although metallic orthopaedic materials have a shared application history with the aerospace industry, their suitability in bone tissue replacements and regenerative therapies remains unchallenged due to their superior mechanical properties. However, improvement in biocompatibility is crucial for the development of the next generation of metallic biomaterials.
PROGRESS IN MATERIALS SCIENCE
(2023)
Review
Engineering, Manufacturing
Amit Bandyopadhyay, Indranath Mitra, Jose D. Avila, Mahadev Upadhyayula, Susmita Bose
Summary: Porous and functionally graded materials have wide applications in biomedical devices, especially in orthopedic and dental implants, due to their desirable mechanical, corrosive, and biocompatible properties. Among various porous materials, porous polymers lack mechanical strength, porous ceramics are brittle, while porous biocompatible metals show tailorable strength and toughness. This review discusses and compares different manufacturing methods for porous or functionally graded metals, exploring their effects on microstructure, composition, porosity, biocompatibility, and mechanical properties. The understanding gained from these investigations in bone implant applications can also be extended to other devices beyond the biomedical field.
INTERNATIONAL JOURNAL OF EXTREME MANUFACTURING
(2023)
Article
Engineering, Biomedical
Masud Rana, Santanu Karmakar, Amit Bandyopadhyay, Amit Roychowdhury
Summary: This paper presents an approach for designing patient-specific implants with inhomogeneous porosity, which can effectively solve the problem of stress shielding in orthopaedic implants. By using orthotropic auxetic structures and computed optimization, the proposed approach reduced stress shielding and improved osseointegration.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Engineering, Biomedical
Ali Afrouzian, Amit Bandyopadhyay
Summary: This study used directed energy deposition (DED) as an additive manufacturing technique to create ceramic-reinforced composites of Ti6Al4V with hydroxyapatite, alumina, and silicon nitride. The composites had tailored microstructures to improve bio-tribological and antibacterial properties. Different composites were made with various ceramic reinforcements, and microstructural observations showed good bonding and increased hardness. The Si3N4-added composites also demonstrated antibacterial response against Staphylococcus aureus. These multifunctional ceramic-reinforced composites are suitable for biomedical devices like hip implants.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Multidisciplinary Sciences
Lile Squires, Ethan Roberts, Amit Bandyopadhyay
Summary: This study demonstrates a complex radial interface concept with in situ mechanical interlocking, resulting in a prestressed compressive effect. The difference in thermal expansion coefficient between stainless steel and mild steel creates residual stresses. The bimetallic structures show a significant improvement in compressive strength compared to monolithic structures.
NATURE COMMUNICATIONS
(2023)
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)
