4.6 Article

Bio-templated CdSe quantum dots green synthesis in the functional protein, lysozyme, and biological activity investigation

Journal

MATERIALS CHEMISTRY AND PHYSICS
Volume 137, Issue 2, Pages 580-585

Publisher

ELSEVIER SCIENCE SA
DOI: 10.1016/j.matchemphys.2012.10.005

Keywords

Semiconductors; Chemical synthesis; Electron microscopy; Fourier transform infrared spectroscopy (FTIR) Photoluminescence spectroscopy; Thermogravimetric analysis (TGA)

Funding

  1. National Natural Science Foundation of China [51175394]
  2. Fundamental Research Funds for the Central Universities [2011-IV-028, 2011-Ia-035]

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Bifunctional fluorescence (CdSe Quantum Dots) - protein (Lysozyme) nanocomposites were synthesized at room temperature by a protein-directed, solution-phase, green-synthetic method. Fluorescence (FL) and absorption spectra showed that CdSe QDs were prepared successfully with Lyz. The average particle size and crystalline structure of QDs were investigated by high-resolution transmission electron microscopy (HRTEM) and X-ray diffraction (XRD), respectively. With attenuated total reflection-fourier transform infrared (ATR-FTIR) spectra and thermogravimetric (TG) analysis, it was confirmed that there is interaction between QDs and amide I, amide II groups in Lyz. FL polarization was measured and FL imaging was done to monitor whether QDs could be responsible for possible changes in the conformation and activity of Lyz. Interestingly, the results showed Lyz still retain the biological activity after formation of QDs, but the secondary structure of the Lyz was changed. And the advantage of this synthesis method is producing excellent fluorescent QDs with specifically biological function. (C) 2012 Elsevier B.V. All rights reserved.

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