Article
Engineering, Biomedical
Daiwei Si, Bo Su, Jingwei Zhang, Kui Zhao, JinMeng Li, DeChun Chen, ShiQi Hu, Xintao Wang
Summary: This study investigated the effects of low-level laser therapy (LLLT) using three different irradiation methods on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in vitro. The results indicated that covering irradiation was the most favorable method for promoting BMSCs proliferation and osteogenic differentiation.
LASERS IN MEDICAL SCIENCE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Afsaneh Rahmati, Roshanak Abbasi, Rezvan Najafi, Loghman Rezaei-Soufi, Hamed Karkehabadi
Summary: This experimental study shows that diode low-level laser and red LED irradiation can enhance the osteogenic differentiation of stem cells from the apical papilla (SCAPs) without adversely affecting cell viability.
Article
Engineering, Biomedical
Carolina S. Santinoni, Adrieli P. C. Neves, Breno F. M. Almeida, Natalia C. Kajimoto, Natalia M. Pola, Eliana A. Caliente, Eduarda L. G. Belem, Joilson B. Lelis, Stephen E. Fucini, Michel R. Messora, Valdir G. Garcia, Suely R. M. Bomfim, Edilson Ervolino, Maria J. H. Nagata
Summary: The combination of BMA and LLLT enhances bone healing by improving the expression of VEGF, PCNA, Runx2, BMP-2, OPN, and OCN.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Cell & Tissue Engineering
Fiona R. Passanha, Madeleine L. Divinagracia, Vanessa L. S. LaPointe
Summary: Cadherin-11 regulates the activity of RTKs in hMSCs, affecting the activation of the MAPK pathway and cell proliferation.
Article
Biochemistry & Molecular Biology
Eun-Ju Lee, Khurshid Ahmad, Shiva Pathak, SunJu Lee, Mohammad Hassan Baig, Jee-Heon Jeong, Kyung-Oh Doh, Dong-Mok Lee, Inho Choi
Summary: This study introduces novel peptides derived from fibronectin that enhance cell adhesion, proliferation, and differentiation, offering a new approach to cell culture. The peptides showed significant effects on primary cells and stem cells, increasing proliferation and promoting differentiation into specific cell types such as osteoblasts. The results suggest potential applications in the production of core materials for cell culture such as scaffolds and culture medium components.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Computer Science, Information Systems
Hongjun Wu, Ziyang Zang, Zhenhua Pan, Jia Shi, Hongli Chen, Zhibo Han, Jianquan Yao
Summary: The study found that LLLT can enhance the differentiation ability of stem cells, improve antioxidant capacity, and does not cause inflammation in normal cells. LLLT combined with inducers at 808 nm can promote the expression of neural precursor markers, while it shows more modest effects at 635 nm.
Article
Nanoscience & Nanotechnology
Zijun Deng, Weiwei Wang, Xun Xu, Yan Nie, Yue Liu, Oliver E. C. Gould, Nan Ma, Andreas Lendlein
Summary: The use of a PDA-coated substrate can reduce oxidative stress and cellular senescence in stem cell expansion, while promoting proliferation rate. PDA also helps suppress the expression of senescence-associated inflammatory components, while maintaining stem cell characteristics and differentiation potential.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Medicine, Research & Experimental
Xiao-Fei Zhang, Ting Wang, Zi-Xuan Wang, Kun-Peng Huang, Yun-Wei Zhang, Guo-Liang Wang, Hong-Ji Zhang, Zi-Han Chen, Chang-Yan Wang, Jin-Xiang Zhang, Hui Wang
Summary: In a hypoxic microenvironment, ucMSCs enhance endothelial cell proliferation and migration through exosome-mediated paracrine function, accelerating wound healing. miR-125b in hypoxic exosomes targets and suppresses TP53INP1, alleviating hypoxia-induced cell apoptosis and promoting cutaneous repair.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Biochemistry & Molecular Biology
Qunzi Ge, Xuemei Wang, Yu Luo, Xiaowen Zheng, Lie Ma
Summary: This study successfully improved the adhesion, spreading, and stemness of rat bone marrow-derived mesenchymal stem cells (rBMSCs) by fabricating E7-modified substrates on PEGylated substrates, showing better results especially on substrates with higher E7 densities.
MACROMOLECULAR BIOSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Haslinda Abdul Hamid, Vahid Hosseinpour Sarmadi, Vivek Prasad, Rajesh Ramasamy, Azizi Miskon
Summary: With the rapid progress in the fields of osteo- and musculoskeletal therapies, the demand for MSC-based treatment modalities is increasing. Electromagnetic field (EMF) exposure is a promising alternative approach. This review discusses the efficiency, practicability, and limitations of relevant methods, providing insurmountable evidence for the implementation of EMF as a feasible strategy for the expansion of MSCs.
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2022)
Review
Multidisciplinary Sciences
Wang yan Pei, Mohamad arif awang Nawi, Norma AB Rahman
Summary: This article reviews the progress in understanding the effects of low-level laser therapy (LLLT) on mesenchymal stem cells (MSC) and the factors that influence these effects. LLLT has been found to promote MSC proliferation and osteogenic differentiation, making it a valuable tool in bone tissue engineering. Factors such as cell type, culture medium, irradiation duration, frequency, spot size, and light flux distribution can affect the biological effects of LLLT on MSC. However, there are limitations in the existing research due to variations in experimental conditions and the inability to consider all influencing factors simultaneously.
Article
Engineering, Biomedical
Liying Wang, Chen Liu, Fan Wu
Summary: The study showed that low-level laser therapy (LLLT) can promote the proliferation and osteogenesis of periodontal ligament stem cells (PDLSCs) at certain energy intensities, but inhibits osteogenic differentiation at high energy intensity, while stimulating the secretion of inflammatory factors. Furthermore, LLLT modulates the proliferation and osteogenic capacity of PDLSCs through the BMP/Smad signaling pathway.
LASERS IN MEDICAL SCIENCE
(2022)
Review
Engineering, Biomedical
Jay R. K. Samal, Vignesh K. Rangasami, Sumanta Samanta, Oommen P. Varghese, Oommen P. Oommen
Summary: Studies have shown that culturing MSCs in a low oxygen environment can upregulate stem cell markers and promote cell proliferation in an undifferentiated state. However, there is ambiguity in the results due to differences in oxygen gradients and culture conditions among different studies. This progress report focuses on conflicting findings related to the application of hypoxic conditions for improving MSC proliferation or differentiation, aiming to decipher the factors influencing MSC characteristics under hypoxia.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Gunhyu Bae, Myeong Soo Kim, Ramar Thangam, Thomas Myeongseok Koo, Woo Young Jang, Jinho Yoon, Seong-Beom Han, Letao Yang, Seong Yeol Kim, Nayeon Kang, Sunhong Min, Hyunsik Hong, Hong En Fu, Min Jun Ko, Dong-Hwee Kim, Woong Kyo Jeong, Dong-Hyun Kim, Tae-Hyung Kim, Jeong-Woo Choi, Ki-Bum Lee, Ramasamy Paulmurugan, Yangzhi Zhu, Han-Jun Kim, Junmin Lee, Jong Seung Kim, Ali Khademhosseini, Young Keun Kim, Heemin Kang
Summary: The study focuses on tuning the diameter and interdistance of RGD-bearing AuNPs on Fe3O4 nanotemplates to regulate stem cell differentiation and adhesion functions.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Fanlu Wang, Lena Marie Saure, Fabian Schuett, Felix Lorich, Florian Rasch, Ali Shaygan Nia, Xinliang Feng, Andreas Seekamp, Tim Klueter, Hendrik Naujokat, Rainer Adelung, Sabine Fuchs
Summary: Graphene oxide shows promising potential in bone tissue engineering, but its molecular biological effects in clinically applied materials are still limited. This study compares the effects of graphene oxide framework structures (F-GO) and reduced graphene oxide-based framework structures (F-rGO) on bone vascularization processes. Non-reduced graphene oxide in the form of F-GO is favored for bone regeneration applications, and graphene oxide coating on a clinically used bone graft improves cell adhesion and vascularization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Sport Sciences
Andre Rodrigues Lourenco Dias, Katrice Almeida de Souza, Kamila Meireles dos Santos, Romulo Mariano de Miranda, Andrey Jorge Serra, Tiago Pecanha, Jacielle Carolina Ferreira, Lucieli Teresa Cambri, Gisela Arsa
Summary: This study compared the 24-hour ambulatory heart rate variability in young overweight and obese men following high-intensity interval exercise (HIIE) and moderate-intensity continuous exercise (MICE), finding no significant differences between the two. However, HIIE was shown to increase cardiac autonomic modulation during the sleep period as a protector of the cardiovascular system, despite its small effect.
EUROPEAN JOURNAL OF SPORT SCIENCE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Daniele Peron, Renato Araujo Prates, Ednei Luiz Antonio, Ighor Luiz Azevedo Teixeira, Helenita Antonia de Oliveira, Barbara Sampaio Dias Martins Mansano, Alexandre Bergamo, Dirceu Rodrigues Almeida, Rafael Dariolli, Paulo Jose Ferreira Tucci, Andrey Jorge Serra
Summary: This study demonstrates that inoculation of Porphyromonas gingivalis may lead to cardiac remodeling and dysfunction in rats, suggesting a potential role of this bacterium in the pathogenesis of heart failure.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
Article
Peripheral Vascular Disease
Ariana A. Silva, Mauro S. Perilhao, Leslie Andrews Portes, Andrey J. Serra, Paulo Jose Ferreira Tucci, Andre Soares Leopoldo, Leonardo Dos Santos, Danilo S. Bocalini
Summary: This study evaluated the effect of exercise on cardiac remodelling in chronically stressed rats. The results showed that while exercise reduced blood pressure and prevented an increase in corticosterone levels, it was unable to impede the myocardial damages caused by chronic stress.
JOURNAL OF HYPERTENSION
(2022)
Article
Dermatology
Regiane dos S. Feliciano, Martha T. Manchini, Allan L. B. Atum, Gabriel Almeida Silva, Ednei L. Antonio, Andrey J. Serra, Paulo J. F. Tucci, Ramon Andrade de Mello, Maria C. Chavantes, Ovidiu C. Baltatu, Jose A. Silva Junior
Summary: This study aimed to assess the temporal effects of Photobiomodulation therapy (PBMT) on cardiac fibrosis activation after myocardial infarction (MI) in rats. The results suggest that PBMT can interfere in cardiac fibrosis activation after MI by reversing the signaling pathway of profibrotic genes, through modulation of gene transcription and miRNA expressions.
LASERS IN SURGERY AND MEDICINE
(2022)
Article
Sport Sciences
A. R. L. Dias, B. L. De Melo, A. A. Dos Santos, J. M. A. Silva, G. Leite, D. S. Bocalini, A. A. Marcolongo, A. J. Serra
Summary: This study found that pole dance athletes have higher physical fitness but no significant changes in heart rate variability markers. Additionally, pole dance athletes showed left ventricle remodeling with improved LV relaxation.
Article
Physiology
Leslie Andrews Portes, Alexandra Alberta dos Santos, Carlos Roberto Padovani, Natalia Cristina de Oliveira, Andrey Jorge Serra, Paulo J. F. Tucci
Summary: Swimming training can favorably affect myocardial calcium responsiveness in rats with moderate and large myocardial infarctions.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Allan Luis Barboza Atum, Leonardo Paroche de Matos, Bruna Calixto de Jesus, Guilherme Rabelo Nasuk, Gabriel Almeida da Silva, Caio Perez Gomes, Joao Bosco Pesquero, Stella Regina Zamuner, Jose Antonio Silva Junior
Summary: The impact of prenatal alcohol exposure (PAE) varies between individuals, leading to morphological and genetic changes that are often undetected in PAE children. This study investigated the effects of PAE on gene transcription related to cardiac dysfunction signaling in mouse myocardium and morphological changes. PAE animals demonstrated changes in gene expression, increased ventricular size, and increased antioxidant protection in the myocardium.
Article
Biochemistry & Molecular Biology
Barbara Sampaio Dias Martins Mansano, Vitor Pocani da Rocha, Ighor Luiz Azevedo Teixeira, Helenita Antonia de Oliveira, Stella Souza Vieira, Ednei Luiz Antonio, Paulo Jose Ferreira Tucci, Andrey Jorge Serra
Summary: This study investigated the influence of red light-emitting diodes (LED, 630 nm) on the metabolism and paracrine factors of adipose tissue-derived mesenchymal stem cells (AdMSCs) under different irradiation parameters and the number of applications. The results showed that three LED irradiations with 4 J cm(-2) may be a suitable parameter for future AdMSCs therapy due to its improved metabolic activity, ATP content, and secretion of IL-6, IGF-1, and nitric oxide.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Gabriel Almeida da Silva, Allan Luis Barboza Atum, Leonardo Paroche de Matos, Guilherme Rabelo Nasuk, Bruna Calixto de Jesus, Telma Luciana Furtado Gouveia, Ovidiu Constantin Baltatu, Stella Regina Zamuner, Jose Antonio Silva Junior
Summary: This study analyzed the effects of prenatal alcohol exposure on the expression of peptidergic systems in the hippocampus and heart of mice. The results showed that alcohol exposure can modulate the renin-angiotensin system and kallikrein-kinin system in these tissues. Additionally, females exposed to prenatal alcohol showed reduced susceptibility to the effects of alcohol.
Editorial Material
Physiology
Andrey Jorge Serra, Stella de Sousa Vieira, Luis Felipe Neves Dos Santos
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Physiology
Jairo Montemor Augusto Silva, Ednei Luiz Antonio, Luis Felipe Neves Dos Santos, Andrey Jorge Serra, Regiane Santos Feliciano, Jose Antonio Silva Junior, Silvia Saiuli Miki Ihara, Paulo Jose Ferreira Tucci, Valdir Ambrosio Moises
Summary: This study aimed to evaluate the morphological, functional, and gene expression parameters of the right ventricle (RV) in rats subjected to pulmonary artery banding (PAB) and analyze the influence of RV remodeling on left ventricular (LV) geometry, histology, gene expression, and functional performance. The results showed that PAB induced RV remodeling with hypertrophy, accompanied by alterations in gene expression and fibrosis. Additionally, the study revealed histological and gene expression changes in the LV that were concurrent with the alterations in the RV.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Oncology
Katia Roque, Rossana Ruiz, Luis Mas, Daniel Humberto Pozza, Marina Vancini, Jose Antonio Silva, Ramon Andrade de Mello
Summary: Immunotherapy has revolutionized the management of advanced non-small cell lung cancer (NSCLC) and plays a crucial role in treating patients without oncogenic driver mutations. This review provides an overview of immunotherapy treatment options, discusses practical considerations, and highlights the latest advances in the field. The complexity of decision-making in immunotherapy arises from the lack of direct prospective comparisons, but analysis of long-term efficacy and safety data can offer valuable insights into optimizing treatment for different patient subsets.
Article
Computer Science, Information Systems
Thiago Bulhoes Da Silva Costa, Felipe Meneguitti Dias, Diego Armando Cardona Cardenas, Marcelo Arruda Fiuza De Toledo, Daniel Mario De Lima, Jose Eduardo Krieger, Marco Antonio Gutierrez
Summary: Cardiovascular diseases are the leading causes of death, and blood pressure monitoring plays a crucial role in prevention, diagnosis, assessment, and treatment. Previous studies on photoplethysmography (PPG) for blood pressure measurement showed overly optimistic results due to limitations in their train/test split configuration. Our study demonstrates that intra-subject data arrangements outperform inter-subject arrangements regardless of the algorithm used, and using only demographic attributes can achieve comparable results to intra-subject scenarios. These findings suggest that algorithms without a calibration strategy in an intra-subject setting may be identifying patients rather than predicting blood pressure.
Article
Computer Science, Information Systems
Felipe Meneguitti Dias, Estela Ribeiro, Ramon Alfredo Moreno, Adele Helena Ribeiro, Nelson Samesima, Carlos Alberto Pastore, Jose Eduardo Krieger, Marco Antonio Gutierrez
Summary: In this study, an artificial intelligence-driven screening system was developed to analyze 12-lead ECG images. The results showed that the method achieved efficient classification of ECG exams, offering a promising solution for the rapid assessment and prioritization of patients in the emergency room.