Review
Food Science & Technology
Chanez Bennacef, Stephane Desobry, Laurent Probst, Sylvie Desobry-Banon
Summary: Encapsulation is a technology used in various industries to protect active molecules and control their release. Coextrusion encapsulation methods can produce core-shell capsules. This review focuses on the applications of coextrusion encapsulation for preserving oils, essential oils, probiotics, and other bioactives. This technology isolates the active molecules, enhances their stability, and allows controlled release. Coextrusion offers valuable opportunities for preserving active molecules in pharmaceutical, food, and agriculture sectors.
Article
Materials Science, Multidisciplinary
Xingxing Liu, Liheng Gao, Sijia Fu, Wenshuo Zhao, Fujun Wang, Jing Gao, Chaojing Li, Hao Wu, Lu Wang
Summary: The management of the interface between the wound microenvironment and the skin substitute (SSS) is essential for wound repair and tissue regeneration. A polycaprolactone fiber-alginate hydrogel interpenetrated network scaffold with multifunctional modifications has been developed. The scaffold exhibits excellent water absorption, water vapor permeability, and stretching properties. It also has antibacterial and reactive oxygen scavenging properties, as well as the ability to promote fibroblast cell proliferation. This scaffold is expected to be an ideal candidate for repairing various types of damaged skin, especially those with excessive exudate.
MATERIALS & DESIGN
(2023)
Article
Chemistry, Multidisciplinary
Alvaro Garcia-Romero, Daniel Miguel, Dominic S. Wright, Celedonio M. Alvarez, Raul Garcia-Rodriguez
Summary: This paper explores the use of main group elements in supramolecular chemistry, specifically in supramolecular porphyrin-based capsules. By studying the periodic trends in the p-block, the authors demonstrate how size and structural control of the capsules can be achieved. The interaction between heavier Group 15 tris(3-pyridyl) linkers and metallo-porphyrins has been investigated, revealing the influence of bond length and oxidation state on the dimensions and reactivity of the capsules.
Review
Chemistry, Multidisciplinary
Anna S. Vikulina, Jack Campbell
Summary: An undeniable trend in modern bioengineering and nanotechnology is the use of various biomolecules, primarily polymeric, for designing novel functional materials in controlled drug delivery, bioimaging, tissue engineering, and other bioapplications. Biogenic polymers with features like biocompatibility, biodegradability, replicating natural cellular microenvironments, and minimal toxicity, have gained growing interest as building blocks for fourth-generation biomaterials. Recent studies highlight the promise of hard-templating approach in fabricating nano- and microparticles using biopolymers, covering up-to-date knowledge on this topic and assessing the progress and challenges in the field.
Article
Chemistry, Multidisciplinary
Yu-Jie Zhu, Ya Gao, Min-Min Tang, Julius Rebek, Yang Yu
Summary: Supramolecular capsules are nanoscale containers used for studying molecular behavior, with practical applications in various fields. Self-assembly is an effective approach in constructing these capsules, utilizing noncovalent interactions. Bowl-shaped cavitand structures made from resorcinarenes have advantages for building supramolecular capsules, especially when held together by unconventional halogen and chalcogen bonds.
CHEMICAL COMMUNICATIONS
(2021)
Article
Polymer Science
Joel Rengifo, Stefan Zschoche, Brigitte Voit, Juan Carlos Rueda
Summary: In this study, new interpenetrated hydrogels sensitive to pH and temperature were synthesized through sequential free radical polymerizations in aqueous medium. These hydrogels showed different contraction or expansion behaviors under different conditions, making them potential actuators and sensor materials.
EUROPEAN POLYMER JOURNAL
(2022)
Review
Chemistry, Medicinal
Chanez Bennacef, Sylvie Desobry-Banon, Laurent Probst, Stephane Desobry
Summary: This paper provides an overview of coextrusion methods for encapsulation, exploring the different techniques available and their potential applications in various industries. The study examines four specific methods, discussing their parameters and the size of capsules they can produce. Coextrusion technology is a promising encapsulation technique for preserving active molecules and has significant economic implications.
Article
Chemistry, Applied
Shuifang Mao, Yanming Ren, Shiguo Chen, Donghong Liu, Xingqian Ye, Jinhu Tian
Summary: In this study, a sodium alginate/protocatechuic acid/iron hydrogel was prepared by rapid self-assembly of protocatechuic acid and Fe3+ under different pH conditions, without the addition of other chemical reagents. The structure of the hydrogel was characterized and the results showed significant differences in the structures prepared at different pH values. The hydrogel exhibited higher gel strength, water holding capacity, and thermal stability, and was able to control the release of bioactive substances in simulated gastrointestinal conditions.
CARBOHYDRATE POLYMERS
(2023)
Review
Biochemistry & Molecular Biology
Minke Yang, Shaojie Zhao, Chengying Zhao, Jiefen Cui, Yanqi Wang, Xiang Fang, Jinkai Zheng
Summary: In this study, protein-reinforced pectin hydrogel beads were developed to overcome the limitations of porous networks and poor mechanical properties. HBPEC-CAS showed a compact network structure and high encapsulation efficiency of EGC, with the ability to delay its release in an aqueous environment and ensure sufficient release in a simulated gastrointestinal environment. Furthermore, EGC was chemically stabilized in HBPEC-CAS through the inhibition of its epimerization, oxidation, dimerization, and trimerization. These findings offer valuable insights for the tea-based food and beverage industry.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Chen Tan, Jing Wang, Baoguo Sun
Summary: The study fabricated dual coated yeast capsules for anthocyanin encapsulation and stabilization, finding that the combination of positively charged chitosan and negatively charged chondroitin sulfate conferred the highest efficiency. These coated yeast capsules exhibited high tolerance to environmental stresses and may hold promise for controlled delivery of water-soluble bioactive components.
Article
Chemistry, Multidisciplinary
Sihang Liu, Liangbo Xu, Zhefan Yuan, Mei Huang, Tian Yang, Shengfu Chen
Summary: The design of a spatial structure for three-dimensional crosslinked units is important for improving the mechanical properties of hydrogels. In this study, a pressure-responsive crosslinker called mGOa was developed, which achieved both low elastic modulus and high compression stress through interlayer sliding. The hydrogel crosslinked by mGOa showed significantly enhanced mechanical strength compared to other crosslinkers.
Article
Biotechnology & Applied Microbiology
Jaqueline Brandao de Souza, Gustavo dos Santos Rosa, Mariana Correa Rossi, Fernanda de Castro Stievani, Joao Pedro Hubbe Pfeifer, Andre Massahiro Teramoto Krieck, Ana Livia de Carvalho Bovolato, Carlos Eduardo Fonseca-Alves, Vicente Amigo Borras, Ana Liz Garcia Alves
Summary: The study evaluated the feasibility of encapsulating adipose tissue-derived mesenchymal stem cells (MSCs) of mules in sodium alginate, showing good capsule morphology, high cell viability, stable immunophenotype, and proper nutrient diffusion. Encapsulation in sodium alginate hydrogel is a feasible technique that preserves the undifferentiated status of stem cells and can be a relevant option for further studies on tridimensional culture systems and in vivo bioactive agents delivery.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Maria C. Gomes, Dora C. S. Costa, Claudia S. Oliveira, Joao F. Mano
Summary: The study developed a protein-based liquefied capsule inspired by adhesive proteins secreted by mussels, allowing for compartmentalization of living cells through simple methodologies. The platform demonstrated good biocompatibility and mechanical properties, showing potential for applications in tissue engineering, regenerative medicine, and other biotechnology fields.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Physical
Liangdong Liu, Jingjing Wei, Kin Man Ho, Kwan Yeung Chiu, To Ngai
Summary: A method for encapsulating sensitive ingredients and protecting their activity was proposed. By changing the template and crosslinking method of the capsules, the active ingredients were successfully encapsulated and protected. This method has great potential for applications in the food, drug, and cosmetic industries.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Physical
Margot Stasse, Tiphaine Ribaut, Valerie Heroguez, Veronique Schmitt
Summary: The research aims to encapsulate fragrances to slow down diffusion, using a unique strategy of developing capsules through water intermediate phase polymerization in a double emulsion system. The obtained 3D network acts as an effective barrier limiting fragrance diffusion, without affecting the fragrance itself.
COLLOID AND POLYMER SCIENCE
(2021)
Article
Medicine, Research & Experimental
Jessie M. Barra, Veronika Kozlovskaya, Jessica D. Kepple, Karen L. Seeberger, Purushothaman Kuppan, Chad S. Hunter, Gregory S. Korbutt, Eugenia Kharlampieva, Hubert M. Tse
Summary: The study demonstrated the efficacy of encapsulating neonatal porcine islets to decrease inflammatory innate immune signals and improve islet function following xenotransplantation. The encapsulation material suppressed reactive oxygen species synthesis and modulated immune responses, leading to restoration of euglycemia in experimental mice.
XENOTRANSPLANTATION
(2021)
Article
Materials Science, Multidisciplinary
Maksim Dolmat, Veronika Kozlovskaya, Donald Cropek, Eugenia Kharlampieva
Summary: A method for obtaining nanothin free-floating PMAA hydrogel films was developed through the dissolution of a sacrificial SiO2 layer. The mechanical properties and strength of the hydrogel films produced by this method were found to be good.
ACS APPLIED POLYMER MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Veronika Kozlovskaya, Eugenia Kharlampieva
Summary: The anisotropy in the shape of polymeric particles offers numerous advantages over spherical particles and has promising applications in biomedicine. The layer-by-layer assembly method allows for precise control over the chemical and physical properties of anisotropic particles, making them versatile and useful in various fields.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Polymer Science
Veronika Kozlovskaya, Bing Xue, Maksim Dolmat, Eugenia Kharlampieva
Summary: The study demonstrates the synthesis of cubical PMAA hydrogel capsules that collapse completely and reversibly in response to osmotic pressure changes induced by PSS. The deformation behavior of the capsules, controlled by network crosslink density, PMAA ionization, and PSS concentration, shows a uniform response to applied pressures with initial rigidity points such as vertices and edges. This research provides fundamental knowledge on the elastic deformations of nonspherical hydrogels, which can be crucial in the development of adaptable systems in controlled delivery, sensing, and microfluidics.
Article
Chemistry, Multidisciplinary
Maksim Dolmat, Claire Thomas, Veronika Kozlovskaya, Eugenia Kharlampieva
Summary: This experiment introduces upper-division undergraduate and graduate students to various concepts in polymer chemistry, materials science, and drug delivery through the synthesis of alginate hydrogel beads and dye encapsulation. The students learn to regulate the size of the beads and explore their properties using different methods. This lab experience not only enhances students' experimental skills but also provides insights into practical applications in industrial and academic research.
JOURNAL OF CHEMICAL EDUCATION
(2022)
Article
Chemistry, Multidisciplinary
Veronika Kozlovskaya, Maksim Dolmat, Eugenia Kharlampieva
Summary: This article highlights the recent advances in assembly technologies, fundamentals, and applications of multilayer hydrogels. It discusses the relationships between synthetic strategies, chemical compositions, and hydrogel architectures, and their effects on stimuli-induced volume changes, morphology, and mechanical responses. The article also presents potential applications of pH- and temperature-sensitive multilayer hydrogels in sensing and drug delivery.
Article
Polymer Science
Maksim Dolmat, Veronika Kozlovskaya, Daniel Inman, Claire Thomas, Eugenia Kharlampieva
Summary: This study developed a dynamic assembly of hydrogen-bonded multilayers and compared them to static multilayers. The dynamic multilayers showed a 15-time faster deposition rate and a 30% increase in thickness and roughness compared to static multilayers. The properties of the coatings, including mechanical properties, wettability, hydration, and pH stability, were insignificantly affected by the assembly mode.
JOURNAL OF POLYMER SCIENCE
(2023)
Article
Surgery
Jessie M. Barra, Veronika Kozlovskaya, KaLia S. Burnette, Ronadip R. Banerjee, Christopher A. Fraker, Eugenia Kharlampieva, Hubert M. Tse
Summary: The study explores the use of islet encapsulation technologies to enhance graft survival and function in type 1 diabetes without systemic immunosuppression. The encapsulation with TA, PVPON, and CTLA-4-Ig leads to localized immunosuppression and prolongs islet allograft function. This is achieved by suppressing proinflammatory macrophage and T cell responses, enhancing regulatory T cells, and inhibiting T cell activation.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Biochemistry & Molecular Biology
Veronika Kozlovskaya, Maxwell Ducharme, Maksim Dolmat, James M. Omweri, Volkan Tekin, Suzanne E. Lapi, Eugenia Kharlampieva
Summary: In this study, 89Zr-labeled triblock copolymer polymersomes were successfully prepared through chelator-free radiolabeling. The polymersomes were modified with a degradable tannin, tannic acid (TA), and a targeting recombinant humanized monoclonal antibody, trastuzumab (Tmab), for specific targeting of cancer cells. This noncovalent modification method could allow easy integration of a variety of targeting proteins and has potential applications in cancer therapy.
Article
Chemistry, Medicinal
Parmanand Ahirwar, Veronika Kozlovskaya, Bhavitavya Nijampatnam, Edwin M. Rojas, Piyasuda Pukkanasut, Daniel Inman, Maksim Dolmat, Anna C. Law, Norbert Schormann, Champion Deivanayagam, Gregory J. Harber, Suzanne M. Michalek, Hui Wu, Eugenia Kharlampieva, Sadanandan E. Velu
Summary: We developed analoguesof biofilm inhibitor IIIC5 to enhance solubility, maintain inhibitory activities, and facilitate encapsulation into pH-responsive hydrogel microparticles. The optimized compound HA5 exhibited improved solubility (120.09 μg/mL), inhibited Streptococcus mutans biofilm formation with an IC50 value of 6.42 μM, and showed no adverse effects on the growth of oral commensal species even at a 15-fold higher concentration. The crystal structure of HA5 in complex with GtfB catalytic domain unveiled its active site interactions. HA5 demonstrated its ability to inhibit S. mutans Gtfs and reduce glucan production. Encapsulating HA5 in hydrogel to form hydrogel-encapsulated biofilm inhibitor (HEBI) achieved selective inhibition of S. mutans biofilms similar to HA5. Treatment of S. mutans-infected rats with HA5 or HEBI resulted in a significant reduction in buccal, sulcal, and proximal dental caries compared to untreated infected rats.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Maksim Dolmat, Veronika Kozlovskaya, Eugenia Kharlampieva
Summary: The inclusion of lab experiments in nanoscience and nanomaterials is essential for expanding the undergraduate curriculum. The use of atomic force microscopy (AFM) allows students to analyze the physical characteristics of polymer blends and crystals at the nanoscale. These experiments provide practical and transferable skills for students to address real-world issues in chemistry and engineering.
JOURNAL OF CHEMICAL EDUCATION
(2023)
Article
Polymer Science
Maksim Dolmat, Veronika Kozlovskaya, John F. Ankner, Eugenia Kharlampieva
Summary: Controlling the internal organization of hydrogel materials allows for precise modulation of their mechanical properties and other physical characteristics. By altering the internal structure of multilayer hydrogel films, researchers have demonstrated the ability to manipulate the swelling and hydration behavior of the hydrogels, thereby changing their mechanical response.
Article
Biochemistry & Molecular Biology
Veronika Kozlovskaya, Maxwell Ducharme, Maksim Dolmat, James M. Omweri, Volkan Tekin, Suzanne E. Lapi, Eugenia Kharlampieva
Summary: Researchers developed a 60 nm-sized polymer vesicle that can be easily imaged using positron emission tomography (PET) without the need for chelators. By modifying the vesicle surface with the degradable tannic acid (TA) and targeting antibody trastuzumab (Tmab), the vesicles were able to specifically target HER2-positive breast cancer cells. These radiolabeled vesicles hold great potential for precise drug delivery and therapy.
Article
Nanoscience & Nanotechnology
Yiming Yang, Veronika Kozlovskaya, Zhuo Zhang, Chuan Xing, Steve Zaharias, Maksim Dolmat, Shuo Qian, Jun Zhang, Jason M. Warram, Eddy S. Yang, Eugenia Kharlampieva
Summary: Theranostic polymeric vesicles were developed for the delivery of PARP1 siRNA to breast cancer tumors, with successful knockdown of PARP1 and significant inhibition of tumor cell proliferation, leading to increased survival in mice.
ACS APPLIED BIO MATERIALS
(2022)
Review
Materials Science, Multidisciplinary
Veronika Kozlovskaya, Maksim Dolmat, Eugenia Kharlampieva
Summary: The mechanical stiffness of polymer particles plays a crucial role in their circulation in blood, cell-particle adhesion, cellular uptake, and biodistribution. Researchers have discussed various approaches to produce micro and nanosized polymeric particulates with controlled mechanical responses, highlighting the potential of regulating particle rigidity by controlling particle shape. The study also reviews measurement methods for testing mechanical responses of polymeric particulates and summarizes recent developments in this field relevant to biomedical applications.
ACS APPLIED POLYMER MATERIALS
(2021)