Article
Nanoscience & Nanotechnology
Itaru Yanagi, Ken-ichi Takeda
Summary: In this study, SiN membranes with thicknesses of 3-7 nm were fabricated using the SiO2 sacrificial layer process. It was found that SiN membranes as thin as 3.07 nm could be successfully formed under certain conditions, and nanopores were fabricated in these membranes via dielectric breakdown. The current noise of the membranes fabricated using the SiO2 sacrificial layer process was approximately 3/5 of that of membranes fabricated using the poly-Si sacrificial layer process.
Article
Multidisciplinary Sciences
Haden L. Scott, Dima Bolmatov, Peter T. Podar, Zening Liu, Jacob J. Kinnun, Benjamin Doughty, Ralph Lydic, Robert L. Sacci, C. Patrick Collier, John Katsaras
Summary: This study demonstrates that droplet interface bilayers (DIBs) composed of lipids can exhibit persistent synaptic plasticity, characterized by long-term potentiation (LTP) and capacitive energy storage. The time scales for the physical changes associated with LTP are longer than previous short-term synaptic plasticity studies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Jing Li, Bintong Huang, Yuanhao Wang, Aijia Li, Yong Wang, Yangyang Pan, Jia Chai, Ze Liu, Yueming Zhai
Summary: The single-molecule technique for investigating unlabeled proteins in solution is challenging, but nanopore sensing offers a label-free tool for collecting structural information. This study developed a reliable method to convert a silicon nitride nanopore into a stable nanonet platform for single-entity sensing. The nanonet provides more structural information and captures the UV-light-induced structural-change process of individual proteins.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Physical
Emilia Krok, Agnieszka Batura, Madhurima Chattopadhyay, Hanna Orlikowska, Lukasz Piatkowski
Summary: Many studies have investigated phase separation and formation of lipid domains, crucial in biological processes. This study examined the formation, dynamics, and stability of phase-separated supported lipid membranes under varying pH conditions, finding a strong correlation between L-o domain size and buffer pH. Lipid dynamics are insensitive to buffer pH, and varying pH can induce specific domain formation without external modification. The lipid membrane architecture remains stable even when the aqueous medium is replaced with neutral pH buffer, making this method applicable for studies involving pH-sensitive molecules.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Multidisciplinary
Andrey Anosov, Polina Astanina, Ivan Proskuryakov, Oksana Koplak, Roman Morgunov
Summary: This study analyzes the structural changes in phosphatidylcholine lipid membranes caused by the introduction of insoluble CoFe2O4 nanoparticles. Changes in nuclear magnetic resonance spectrum, infrared spectrum, and ionic conductivity of membranes were observed with the addition of nanoparticles. The presence of nanoparticles in membranes was confirmed using atomic force and magnetic force microscopy. The structural changes in the membranes near the lipid C-O bonds caused by nanoparticles were observed using scanning near-field optical microscopy. Analysis of nuclear magnetic resonance (NMR) spectra allowed identification of the affected atomic groups in the membrane surface layers. Conductivity measurements of the bilayer membranes were performed in different modes and showed an increase in ionic conductivity depending on the concentration of hydrophobic nanoparticles, which induce surface distortion and pore creation. The concentration dependence demonstrated a percolation threshold, which was analyzed using fractal theory approach.
Article
Chemistry, Analytical
Minggang Tian, Yaru Sun, Xiuqi Kong, Baoli Dong
Summary: Studying the phase separation of ER membranes is crucial for understanding the fundamental biology of ER. However, NIR ratiometric imaging of ER phase behaviors has not been explored in living cells and diverse tissues. In this study, we developed a polarity-responsive NIR fluorescent probe to visualize the phase behavior in ER membranes and revealed different phase behaviors in different tissues.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Jiajun Wang, Yi-Lun Ying, Cheng-Bing Zhong, Li-Min Zhang, Feng Yan, Yi-Tao Long
Summary: Nanopore electrochemistry, a promising tool for single molecule sensing, requires appropriate nanopore sensing interface, advanced instrumentation, and multi-disciplinary collaboration for high resolution and throughput. Recent improvements in the nanopore sensing interface, nanopore arrays, and intelligent data analysis methods have enhanced the potential for widespread application in single-molecule research.
Article
Chemistry, Multidisciplinary
James T. Hagan, Alejandra Gonzalez, Yuran Shi, Grace G. D. Han, Jason R. Dwyer
Summary: In this study, we fabricated photoregulated thin-film nanopores by covalently linking azobenzene photoswitches to silicon nitride pores. The photoresponsive coatings allowed for repeated optical switching of the nanopores, resulting in changes to the effective nanopore diameter and ionic conductance. The sensitivity of the nanopores to specific analytes could be controlled through illumination wavelength and voltage polarity. This research has significant implications for the development of ionic circuit elements and chemically tuned filters, as well as for single-molecule sensing applications in personalized medicine, genomics, and glycomics.
Article
Chemistry, Analytical
Essraa A. Hussein, Ryan J. White
Summary: Resistive pulse sensing using ion channel proteins has evolved as a single-molecule approach with high molecular selectivity, but requires a stable lipid membrane. The newly developed gold electrode-supported bilayer technology offers high spatial resolution but suffers from channel current decay. Introducing a silver nanoneedle to support lipid bilayer formation and ion channel measurements can mitigate current decay and produce stable DC channel currents.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Hammad A. Faizi, Rumiana Dimova, Petia M. Vlahovska
Summary: The bending rigidity and capacitance of biomimetic lipid bilayers can be simultaneously measured using a method that involves ellipsoidal deformation of quasi-spherical giant unilamellar vesicles induced by a uniform AC electric field.
Article
Engineering, Electrical & Electronic
A. Shafikov, B. Schurink, R. W. E. van de Kruijs, J. Benschop, W. T. E. van den Beld, Z. S. Houweling, F. Bijkerk
Summary: By creating compressive residual stress at the membrane surface, the maximum stress induced by the applied pressure was reduced and the fracture strength of the Si3N4 was increased. As a result, membranes with a compressive surface showed a 50% increase in pressure limit.
SENSORS AND ACTUATORS A-PHYSICAL
(2021)
Article
Biochemistry & Molecular Biology
Moynul Hasan, Farzana Hossain, Hideo Dohra, Masahito Yamazaki
Summary: This study investigates the role of interfacial hydrophobicity of antimicrobial peptide Mag in its nanopore formation activity. The results demonstrate that the higher the interfacial hydrophobicity of Mag mutants, the greater the rate of nanopore formation and fractional area change. These findings reveal the important role of interfacial hydrophobicity in the nanopore formation activity of Mag.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Zehui Xia, Andre Scott, Rachael Keneipp, Joshua Chen, David J. Niedzwiecki, Brian DiPaolo, Marija Drndic
Summary: We have demonstrated the translocations of DNA through silicon nitride pores formed by simple chemical etching. The characterization of these nanopores was confirmed through DNA translocations and TEM imaging. Our study provides comprehensive information about the pore area, number, and structure using multiple methods, including ionic conductance and electron energy loss spectroscopy. This affordable chemical method for making solid-state nanopores shows great potential in DNA sensing and characterization applications.
Article
Biophysics
Paige Liu, Oscar Zabala-Ferrera, Peter J. Beltramo
Summary: Cell membranes maintain an electric potential due to the distribution of charged phospholipids across the membrane. Experimental artificial membrane systems allow for isolated examination of membrane properties and processes.
BIOPHYSICAL JOURNAL
(2021)
Article
Chemistry, Physical
Alexander Kiy, Christian Notthoff, Shankar Dutt, Mark Grigg, Andrea Hadley, Pablo Mota-Santiago, Nigel Kirby, Christina Trautmann, Maria E. Toimil-Molares, Patrick Kluth
Summary: In this study, in situ small angle X-ray scattering was used to monitor the ion track etching process in polycarbonate foils, providing detailed information about the etching kinetics and nanopore size and distribution. The results show that the etching process can be described by an Arrhenius law, and radial etching exhibits a power law dependency on etchant concentration.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Engineering, Electrical & Electronic
Parsa Taheri-Tehrani, Anthony Dorian Challoner, David A. Horsley
IEEE SENSORS JOURNAL
(2018)
Article
Engineering, Electrical & Electronic
Yuri Kusano, Qi Wang, Guo-Lun Luo, Yipeng Lu, Ryan Q. Rudy, Ronald G. Polcawich, David A. Horsley
JOURNAL OF MICROELECTROMECHANICAL SYSTEMS
(2018)
Article
Chemistry, Analytical
Andre Guedes, Rita Macedo, Gerardo Jaramillo, Susana Cardoso, Paulo P. Freitas, David A. Horsley
Article
Acoustics
Yuri Kusano, Itaru Ishii, Tatsuya Kamiya, Akihiko Teshigahara, Guo-Lun Luo, David A. Horsley
IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL
(2019)
Article
Engineering, Electrical & Electronic
Parsa Taheri-Tehrani, Martial Defoort, David A. Horsley
JOURNAL OF MICROELECTROMECHANICAL SYSTEMS
(2019)
Article
Engineering, Electrical & Electronic
Guo-Lun Luo, Yuri Kusano, David A. Horsley
Summary: This paper presents a low-frequency airborne piezoelectric micro-machined ultrasonic transducers (PMUTs) for long-range detection, utilizing single-crystal PZT material to achieve high piezoelectric coefficient and low dielectric constant for efficient and high sound pressure level output. Experimental results demonstrate high sensitivity and long-range detection capabilities.
JOURNAL OF MICROELECTROMECHANICAL SYSTEMS
(2021)
Proceedings Paper
Engineering, Electrical & Electronic
Yuri Kusano, Guo-Lun Luo, David A. Horsley
2019 20TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS & EUROSENSORS XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)
(2019)
Proceedings Paper
Engineering, Electrical & Electronic
Guo-Lun Luo, David A. Horsley
2019 20TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS & EUROSENSORS XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)
(2019)
Proceedings Paper
Engineering, Electrical & Electronic
Guo-Lun Luo, Yuri Kusano, Matthew N. Roberto, David A. Horsley
2019 IEEE 32ND INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS)
(2019)
Proceedings Paper
Engineering, Electrical & Electronic
Guo-Lun Luo, Qi Wang, Yuri Kusano, David Horsley
2018 IEEE INTERNATIONAL FREQUENCY CONTROL SYMPOSIUM (IFCS)
(2018)
Proceedings Paper
Engineering, Electrical & Electronic
Parsa Taheri-Tehrani, Martial Defoort, David A. Horsley
2018 IEEE INTERNATIONAL FREQUENCY CONTROL SYMPOSIUM (IFCS)
(2018)
Proceedings Paper
Engineering, Electrical & Electronic
Guo-Lun Luo, Yuri Kusano, David Horsley
2018 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM (IUS)
(2018)
Proceedings Paper
Engineering, Electrical & Electronic
Qi Wang, Yuri Kusano, David A. Horsley
30TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2017)
(2017)
Proceedings Paper
Engineering, Electrical & Electronic
Xiaoyue Jiang, Hao-Yen Tang, Yipeng Lu, Eldwin J. Ng, Julius M. Tsai, Michael J. Daneman, Bernhard E. Boser, David A. Horsley
30TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2017)
(2017)
Proceedings Paper
Engineering, Electrical & Electronic
Yuri Kusano, Qi Wang, Ryan Q. Rudy, Ronald G. Polcawich, David A. Horsley
30TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2017)
(2017)