Journal
LANGMUIR
Volume 27, Issue 17, Pages 10920-10928Publisher
AMER CHEMICAL SOC
DOI: 10.1021/la2019132
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Funding
- EU
- Agency for Science, Technology and Research (Singapore)
- ETH Zurich
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Self-assembly of nanopore-spanning lipid bilayers (npsLBs) paves the way toward chip-based integrated membrane protein biosensing. We present a novel approach to analyze the formation of npsLB at individual nanopores using quantitative analysis of high-resolution microscopy images. From this analysis we derive necessary conditions for the formation of npsLBs on nanopore arrays by liposome fusion and discuss the limitations of the process as a function of nanopore geometry, lipid membrane properties, and surface interaction. Most importantly, applying liposomes with diameters larger than the nanopore is demonstrated to be a necessary but not sufficient condition for npsLB formation. A theoretical model is used to discuss and explain this experimental finding.
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