Article
Chemistry, Multidisciplinary
J. Tucker Andrews, Kirstyn E. Baker, Jacob T. Handloser, Natalie Bridges, Alexis A. Krone, Peter J. N. Kett
Summary: This study used a quartz crystal microbalance to investigate the effect of salt on the formation of phosphatidylcholine SLBs on a silicon dioxide surface, finding that the influence of different cations on SLB formation varies at different salt concentrations.
Article
Multidisciplinary Sciences
Nika Marusic, Lado Otrin, Jonas Rauchhaus, Ziliang Zhao, Fotis L. Kyrilis, Farzad Hamdi, Panagiotis L. Kastritis, Rumiana Dimova, Ivan Ivanov, Kai Sundmacher
Summary: Biomimetic polymer/lipid hybrid compartments have great potential as substitutes for natural liposomes. This study explores the protein-free fusion of these semisynthetic membranes and finds that the membrane mixing of oppositely charged hybrids exceeds that of liposomes. The charge is not the sole determinant of the final adenosine triphosphate synthesis rate, and the fusion strategy can be used for the delivery of membrane proteins and the automation of synthetic cell assembly.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Nathan E. Thomas, Wei Feng, Katherine A. Henzler-Wildman
Summary: This study presents a new method for measuring transport stoichiometry using solid supported membrane electrophysiology (SSME), which greatly improves throughput and allows verification of specific transport ratios for various ion-coupled transporters.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biophysics
Autumn A. Anthony, Osman Sahin, Murat Kaya Yapici, Daniel Rogers, Aurelia R. Honerkamp-Smith
Summary: This study systematically measured the coefficient of interleaflet friction in supported lipid membranes using a microfluidic technique. The results showed that this technique provides reproducible measurements of the friction coefficient and is sensitive enough to detect differences in friction between different lipid membranes. Adding cholesterol significantly increased interleaflet friction. Additionally, fluid shear stress was found to reversibly induce the gel phase in supported lipid bilayers close to the gel-transition temperature.
BIOPHYSICAL JOURNAL
(2022)
Article
Chemistry, Physical
Massimiliano Galluzzi, Lorenzo Marfori, Stefania Asperti, Alessandro De Vita, Matteo Giannangeli, Alessandro Caselli, Paolo Milani, Alessandro Podesta
Summary: Research has shown that Ionic Liquids (ILs) can restructure lipid bilayers by forming new ILs/lipid complexes, leading to distinctive morphological features such as increased area and roughness. Additionally, IL exposure can disrupt the structural ordering and rigidity of membranes, as evidenced by changes in breakthrough force, Young's modulus, and area stretching modulus. AFM analysis also detected alterations in the electrostatic double-layer at the membrane surface, indicating a progressive stratification of cations on the lipid membrane.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Electrochemistry
Inga Gabriunaite, Gintaras Valincius, Albinas Zilinskas, Ausra Valiuniene
Summary: Silane compound synthesized via click chemistry can form self-assembled monolayers on metal oxide conducting films, resulting in electrically insulating tethered bilayer membranes (tBLMs). The tBLMs, containing water-ion reservoir, demonstrate biological relevance and the ability to reconstitute specific protein channels. The addition of cholesterol can mitigate membrane damage caused by certain proteins. This biomimetic membrane system can be regenerated multiple times without losing functionality.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Anna De Angelis, Sang Ho Park, Stanley J. Opella
Summary: Phospholipid bicelles are valuable membrane model systems for studying membrane proteins and n-dodecyl-beta-D-melibioside (DDMB) can produce magnetically alignable lipid bilayers with high cholesterol content.
Article
Chemistry, Multidisciplinary
Ons Hmam, Antonella Badia
Summary: The surface-confined redox reaction triggers the formation of high surface coverage bilayers on gold surfaces. This method is rapid, compatible with various charged phospholipids, and is not affected by the phase state of the phospholipids.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Negin Gooran, Bo Kyeong Yoon, Joshua A. Jackman
Summary: This study comparatively evaluated the membrane-disruptive properties of TX-100 and potential replacement SL-11W using a supported lipid bilayer (SLB) platform. The results showed that TX-100 exhibited higher potency and caused rapid and irreversible membrane solubilization, while SL-11W induced more gradual and reversible membrane budding. The study also revealed that both TX-100 and SL-11W showed concentration-dependent interaction behaviors and were only active at or above their critical micelle concentration (CMC) values.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
A. R. Ortiz Moreno, R. Li, K. Wu, R. Schirhagl
Summary: Lipid peroxidation is a process where reactive oxygen species attack lipids within cell membranes, leading to toxic and carcinogenic products. Understanding this process at nanoscale resolution is important and challenging due to the short lifespan and reactivity of the radical intermediates involved. In this study, lipid peroxidation of three artificial lipid bilayers on a diamond substrate was investigated using a diamond quantum sensing method called T-1-relaxometry, allowing for in situ measurements and imaging of radical intermediates.
Article
Chemistry, Multidisciplinary
Daniel J. Speer, James C. S. Ho, Atul N. Parikh
Summary: This study investigates the solubilization process of the myelin figure by a zwitterionic detergent and reveals that it occurs in a multistep manner, producing morphologically distinct intermediates. These intermediates include coiling, thinning, and detachment or retraction. The appearance of these intermediates suggests a complex free-energy landscape characterizing myelin solubilization.
Article
Materials Science, Multidisciplinary
Konstantinos Kallitsis, Anna-Maria Pappa, Zixuan Lu, Alberto Alvarez-Fernandez, Ioanna Charalambous, Sina Schack, Walther C. Traberg, Quentin Thiburce, Karan Bali, Graham Christie, Stefan Guldin, Susan Daniel, Alberto Salleo, Roisin M. Owens
Summary: This study presents a versatile method to modify the surface chemistry of conducting polymers, aiming to bridge the chemical gap between bioelectronic devices and biological systems. The surface of poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) is modified to enhance the formation of cell-derived supported lipid bilayers (SLBs). The functionalized devices show improved electronic output and demonstrate the potential of this approach in membrane-on-a-chip systems.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2023)
Article
Chemistry, Physical
Daniel J. J. Speer, Marta Salvador-Castell, Yuqi Huang, Gang-Yu Liu, Sunil K. K. Sinha, Atul N. N. Parikh
Summary: The hydrophobic effect drives the self-assembly of amphiphilic structures such as lamellar and micellar mesophases. This study investigates the structural organization of mixtures of phospholipid POPC and two zwitterionic surfactants, DDAPS and O-Lyso-PC. The results reveal the assembly of mixed mesophases with varied structural trends, and the chemical specificity of the surfactant-lipid interactions plays a key role in modulating their properties.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Chemistry, Physical
Nicolas Dolder, Philipp Muller, Christoph von Ballmoos
Summary: Giant unilamellar vesicles (GUVs) are important model membrane systems for studying membrane phenomena and membrane protein functions. However, the existing protocols for GUV formation are diverse and can be influenced by the composition of salt and buffer.
Article
Chemistry, Multidisciplinary
Aoon Rizvi, Justin T. Mulvey, Joseph P. Patterson
Summary: The study investigates the formation of block-copolymer-supported bilayers using LP-TEM, revealing two formation pathways involving liquid droplets or vesicles as intermediates. The results suggest that bilayer assembly methods via liquid droplet intermediates are beneficial for forming pristine supported bilayers, and supported bilayers inside liquid cells may be useful for imaging membrane interactions with proteins and nanoparticles in the future.
Article
Chemistry, Multidisciplinary
Ai Ushiyama, Mio Ono, Chiho Kataoka-Hamai, Tetsushi Taguchi, Yoshihisa Kaizuka
Article
Chemistry, Multidisciplinary
Chiho Kataoka-Hamai, Tomohiko Yamazaki
Article
Chemistry, Multidisciplinary
Chiho Kataoka-Hamai, Yoshihisa Kaizuka, Tetsushi Taguchi
Article
Engineering, Electrical & Electronic
Chiho Kataoka-Hamai, Yuji Miyahara
IEEE SENSORS JOURNAL
(2011)
Article
Biochemistry & Molecular Biology
Gabriela Furlan, Takashi Minowa, Nobutaka Hanagata, Chiho Kataoka-Hamai, Yoshihisa Kaizuka
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Chemistry, Physical
Chiho Kataoka-Hamai, Mahoko Higuchi
JOURNAL OF PHYSICAL CHEMISTRY B
(2014)
Article
Chemistry, Multidisciplinary
Chiho Kataoka-Hamai, Kohsaku Kawakami
Article
Chemistry, Physical
Chiho Kataoka-Hamai, Kohsaku Kawakami
JOURNAL OF PHYSICAL CHEMISTRY B
(2020)
Article
Chemistry, Multidisciplinary
Chiho Kataoka-Hamai, Kohsaku Kawakami
Summary: The study investigated the adsorption of phase-separated GUVs on glass, revealing that both liquid-ordered and liquid-disordered domains are reorganized to stabilize the curved bilayer region in adsorbed GUVs.
Article
Chemistry, Multidisciplinary
Chiho Kataoka-Hamai, Kohsaku Kawakami
Summary: Phospholipid monolayers at oil-water interfaces are used for exploring biological interface properties, with researchers developing methods to quantify mono-layer system characteristics. By calculating surface pressure Pi and multiplying it by estimated values, the dependence of Pi on membrane area a was determined through investigations on POPC monolayers at different interfaces.
Article
Chemistry, Multidisciplinary
Chiho Kataoka-Hamai, Kohsaku Kawakami
Summary: Phospholipid monolayers formed at oil-water interfaces have been studied for various biological applications, with the interaction between the monolayers and hydrocarbons depending on the molecular structure, hydrophobic chain length, and area compression.
Article
Chemistry, Multidisciplinary
Chiho Kataoka-Hamai, Kohsaku Kawakami
Summary: In this study, the adsorption of triacylglycerol droplets onto glass-supported phospholipid bilayers was investigated using fluorescence microscopy. The results showed that triacylglycerol droplets were immobilized in the bilayer membrane and their volume varied over time. Phospholipids on and near the droplets were fully mobile, and triacylglycerol molecules diffused between different droplets along the bilayer. The size distribution of the droplets also changed over time.
Article
Green & Sustainable Science & Technology
Kohsaku Kawakami, Junko Tanaka, Chiho Kataoka-Hamai
Summary: Phospholipid, as a natural and abundant resource, can be used as functional building blocks for various soft materials. In this study, mesoporous phospholipid particles (MPPs) were utilized to stabilize liquid/liquid interfaces and produce oil-in-water emulsions. The MPPs, composed of lipid bilayers and approximately 10 μm in size, showed high surface activity and could adsorb at the oil/water interface to form stable emulsions. The destabilization mechanism of the emulsion depended on the presence of charged molecules on the MPPs, with aggregation occurring in the case of charged MPPs and coalescence in the case of non-charged MPPs. These materials have potential applications as more nature-friendly alternatives to surfactants for oil adsorption/recovery in seawater and soils.
SUSTAINABLE MATERIALS AND TECHNOLOGIES
(2023)
Article
Chemistry, Multidisciplinary
Miyuki Tabata, Chiho Kataoka-Hamai, Kozue Nogami, Daiju Tsuya, Tatsuro Goda, Akira Matsumoto, Yuji Miyahara
Summary: A solid-state potentiometric biosensor based on mixed phase modification of a silver surface was proposed in this study, achieving stable electromotive force and biomolecule functionalization simultaneously. The modified surface reduced nonspecific binding of interfering biomolecules and maintained stability under constant chloride ion concentrations. With a low detection limit of 0.1 pM for microRNA 146, this biosensor could be useful as a disposable single-use sensor in medical fields.
