Article
Chemistry, Analytical
David Ruskic, Frank Klont, Gerard Hopfgartner
Summary: Modifiers play a crucial role in tuning separation in DMS, with different types of modifier mixtures affecting resolution, selectivity, and sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Madhuri Jayathirtha, Emmalyn J. Dupree, Zaen Manzoor, Brianna Larose, Zach Sechrist, Anca-Narcisa Neagu, Brindusa Alina Petre, Costel C. Darie
Summary: The human genome consists of around 30,000 genes, making humans slightly more complex than worms or flies. However, the complexity of humans mainly comes from the diverse proteins encoded by these genes, as well as the production of multiple proteins through alternative splicing and tissue-dependent expression. Post-translational modifications in proteins significantly increase the number of gene products or protein isoforms.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2021)
Article
Chemistry, Medicinal
Monica Iavorschi, Ancuta-Veronica Lupaescu, Laura Darie-Ion, Maria Indeykina, Gabriela Elena Hitruc, Brindusa Alina Petre
Summary: Alzheimer's disease is a progressive neurodegenerative disease characterized by abnormal amyloid depositions in the brain. This study investigated the binding affinity between NAP peptide, Aβ(9-16) fragment, and Cu, Zn ions. The results showed that the Aβ(9-16) fragment can bind metal ions and promote peptide aggregation, while the NAP peptide can inhibit the folding process by chelating metals and interacting with Aβ peptide.
Review
Biochemistry & Molecular Biology
Jenny Leopold, Patricia Prabutzki, Kathrin M. Engel, Juergen Schiller
Summary: In this update on the progress in MALDI-based lipid analysis since 2018, the selection of an appropriate matrix is found to be crucial for optimum results. The complexity of lipid classes increases from free fatty acids to cardiolipins and phosphoinositides over the last five years.
Article
Chemistry, Analytical
Mojgan Sadat Shushtarian, Mohammad Hadi Givianrad, Mohammad Saber Tehrani, Parviz Aberoomand-Azar
Summary: This study developed an efficient strategy utilizing ion imprinted polymer (IIP) based mu-TLC-LA-ICP-MS for speciation analysis of inorganic arsenic. The IIP was characterized using FTIR and FESEM, and a TLC plate was successfully prepared. Under optimized conditions, the method demonstrated a low detection limit and wide linear dynamic range, with good performance in wastewater and human blood plasma samples.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Applied
Xiaojing Sheng, Satoshi Nagaoka, Marika Hashimoto, Yusuke Amiya, Masato Beppu, Kenichiro Tsukamoto, Emiko Yanase, Mitsuru Tanaka, Toshiro Matsui
Summary: Beta-conglycinin was orally administered to rats, and specific oligopeptides derived from the protein were identified in the blood plasma, indicating that beta-conglycinin could be the dietary source protein for these peptides found in the circulating bloodstream of rats.
Article
Biochemical Research Methods
Kai Liang, Qianqian Li, Zhijing Song, Keli Zhao, Rong Su, Shengchun Huang, Xueyan Guo, Yan Li
Summary: Non-cryopreservation temperature exposure can affect plasma quality, and changes in low-molecular-weight peptides can be used as biomarkers to predict sample quality. This study identified 10 peptides that consistently showed time-dependent changes, which can be used as biomarkers for quality control.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Agricultural Engineering
Juana Rozalen, M. Mercedes Garcia, Amaya Zalacain, Horacio Lopez-Corcoles, Jorge Hurtado de Mendoza, Katrina Cornish, Manuel Carmona
Summary: Guayule, as a promising renewable source of natural rubber/latex, has resins with various activities potentially related to sesquiterpenes. By exploring different analytical methods, it was found that there may be new members of the guayulins family and the relationship in seasonal changes was identified.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Chengxue Qu, Weikang Shu, Fengjuan Xie, Yang Liu, Rongxin Li, Congcong Pei, Jingjing Wan
Summary: This paper introduces a peptide profiling method based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which relies on enrichment methods to improve signal intensity. The authors modified SBA-15 with third-generation dendrimers to capture low-abundance peptides efficiently. The synthesized nanocomposites showed enhanced peptide enrichment ability, making them a promising class of nanomaterials for peptide mapping from biological samples.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Organic
Bernard Fernandez, Jean Armengaud, Gilles Subra, Christine Enjalbal
Summary: This study investigates the feasibility of using high energy charge remote fragmentations to analyze the N-terminus of proteins, and compares it with low energy collision-induced dissociations. The results show that the amino acid composition significantly affects the dissociation pathways of peptide backbones.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Review
Chemistry, Analytical
M. Sejalon-Cipolla, P. Bruyat, S. Bregant, C. Malgorn, L. Devel, G. Subra, S. Cantel
Summary: MALDI-MS is crucial for detecting and identifying molecules involved in biological pathways, but many biomarkers remain undetectable due to various limitations. To overcome these challenges, labeling strategies and activity-based probes have been developed. Matrix-free derivatization techniques are currently gaining attention.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Chunxia Ma, Lei Xie, Xiao Wang, Kang Liang, Biao Kong
Summary: Functionalized mesoporous nanomaterials have diverse structural features and unique properties, making them widely applicable in MALDI-MS. Precisely controlling the factors in the assembly of mesoporous nanomaterials is crucial for achieving high-performance MALDI-MS. Significant progress has been made in the fabrication and properties of mesoporous nanomaterials in recent years.
Article
Chemistry, Analytical
Suya Liu, Pavel Ryumin, Jenny Albanese, Zoe Zhang, Takashi Baba
Summary: Here, a novel liquid chromatography coupled with tandem mass spectrometry method was developed to characterize N-acetylneuraminic acid (Neu5Ac, Sa) linkage in N-linked glycans in glycopeptides without sialic acid derivatization. The method involved a reversed-phase high-performance liquid chromatography (HPLC) separation based on Sa linkage and an electron-activated dissociation approach for characterization. The study successfully identified different Sa linkages in N-glycopeptides and proposed a rule for characterization using the Sa-Gal products.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Zhi Qiao, Franziska Lissel
Summary: Researchers have overcome challenges in analyzing low molecular weight compounds using MALDI MS by adjusting sample preparation strategies and developing new matrices. These new matrices not only have high absorption and ionization efficiency, but also ensure vacuum stability and do not produce interfering signals in the low m/z region.
CHEMISTRY-AN ASIAN JOURNAL
(2021)
Article
Agriculture, Multidisciplinary
Xiaojing Sheng, Mitsuru Tanaka, Risa Katagihara, Marika Hashimoto, Satoshi Nagaoka, Toshiro Matsui
Summary: The study established a novel analytical assay for peptide metabolites from glycinin, using MALDI-MS combined with amine derivatization with coumarin. The use of coumarin derivatization under specific conditions led to improved MS detection of peptides, allowing for successful characterization of glycinin-derived metabolites in rats.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Polymer Science
Jacek Walkowiak, Yan Lu, Michael Gradzielski, Stefan Zauscher, Matthias Ballauff
MACROMOLECULAR RAPID COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Ho Shin Kim, Nathanael A. Brown, Stefan Zauscher, Yaroslava G. Yingling
Article
Multidisciplinary Sciences
Stefan Roberts, Vincent Miao, Simone Costa, Joseph Simon, Garrett Kelly, Tejank Shah, Stefan Zauscher, Ashutosh Chilkoti
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Steven G. Noyce, James L. Doherty, Stefan Zauscher, Aaron D. Franklin
Article
Chemistry, Multidisciplinary
Chelsea N. Fries, Yaoying Wu, Sean H. Kelly, Michelle Wolf, Nicole L. Votaw, Stefan Zauscher, Joel H. Collier
ADVANCED MATERIALS
(2020)
Article
Polymer Science
Jacek Walkowiak, Michael Gradzielski, Stefan Zauscher, Matthias Ballauff
Summary: In this study, a PAA brush was prepared by ATRP of tBA and subsequent acid hydrolysis on gold surfaces of QCM crystals. The interaction between the PAA brush and HSA was studied, showing strong protein adsorption onto the brush and the ability to release HSA molecules by increasing ionic strength. Comparison with calorimetric studies allowed for a thorough analysis of the QCM data based on thermodynamic analysis.
Article
Chemistry, Multidisciplinary
Yunqi Yang, Qinyi Lu, Chao-Min Huang, Hongji Qian, Yunlong Zhang, Sonal Deshpande, Gaurav Arya, Yonggang Ke, Stefan Zauscher
Summary: The combination of SI-TcEP with DONs allows for the generation of stable, polynucleotide brush-functionalized DNA nanostructures with precise control over brush location, height, and composition. This results in increased nuclease resistance and spatially programmable stability. Site-specific decoration of DONs with brushes of natural and non-natural nucleotides provides access to a wide range of functionalized DON architectures for potential applications in smart nanoscale delivery systems.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Luis A. Navarro, Justin J. Ryan, Michael Dzuricky, Michael Gradzielski, Ashutosh Chilkoti, Stefan Zauscher
Summary: Research demonstrates that diblock copolymers composed of thermally responsive polypeptides can easily form self-assembled structures driven primarily by polymer-solvent interactions. Temperature has a weak effect on the spacing of resulting nanostructures, while nanostructure spacing is more sensitive to RLP block length.
Article
Biochemistry & Molecular Biology
Miriam Simon, Albert Prause, Stefan Zauscher, Michael Gradzielski
Summary: This study investigates the directed self-assembly of complementary single-stranded DNA strands (poly(dA) and poly(dT)) into complex, organized, and percolating networks in dilute solutions and at surfaces. The knowledge of DNA assemblies in solution and their impact on network formation is crucial for controlling the fabrication of nanotechnological devices. Fluorescence cross-correlation spectroscopy confirms the presence of larger DNA complexes in mixed solutions at low concentrations, emphasizing the importance of considering network precursors in solution.
Article
Engineering, Biomedical
Anna Gilpin, Yuze Zeng, Jiaul Hoque, Ji Hyun Ryu, Yong Yang, Stefan Zauscher, William Eward, Shyni Varghese
Summary: The modification of hyaluronic acid (HA) to exhibit self-healing properties improves lubrication, enhances free radical scavenging, and attenuates enzymatic degradation. In vivo, self-healing HA shows improved retention despite its low molecular weight, and mitigates cartilage degeneration in rodents with anterior cruciate ligament injury. This study demonstrates how incorporating functional properties such as self-healing can enhance the capabilities of biolubricants.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Multidisciplinary Sciences
Michael N. Young, Michael J. Sindoni, Amanda H. Lewis, Stefan Zauscher, Joerg Grandl
Summary: This study combines atomic force microscopy with patch-clamp electrophysiology to determine the physical limits of cells as sensors of mechanical energy. The researchers find that cells can function as proportional or nonlinear transducers of mechanical energy and detect very small mechanical energies with high resolution. They also discover that cells can transduce forces either nearly instantaneously or with a substantial time delay. The study provides insights into the capabilities and limits of cellular mechanosensing and the molecular mechanisms involved.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Yunqi Yang, Qinyi Lu, Yu Chen, Marcello DeLuca, Gaurav Arya, Yonggang Ke, Stefan Zauscher
Summary: DNA nanotechnology allows for the creation of precise and tunable nanostructures for biomedical applications. However, the stability of these structures is compromised in biological environments due to degradation by nucleases. This study demonstrates a strategy to enhance the stability of the structures by utilizing enzymatic polymerization to grow polynucleotide brushes on DNA nanostructures. The ability to control the spatial and temporal growth of these brushes offers potential applications in drug delivery and supramolecular assembly.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Marek Bekir, Christoph Brueckner, Stefan Zauscher, Michael Gradzielski
Summary: This article investigates the adsorption kinetics of positively charged hard and soft silica nanoparticles on supported lipid bilayers with an anionic surface charge. The adsorption process is irreversible and can be described using a Langmuir-like adsorption model. The softness of the nanoparticles can be adjusted by varying the grafting density of covalently bound PDMAEMA brushes. Electrostatic attraction favors nanoparticle adsorption and increases the adsorption kinetics. However, the adsorption kinetics decrease with increasing grafting density of the polyelectrolyte layer, ultimately preventing adsorption at a critical grafting density.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Review
Chemistry, Physical
Gavin Gonzales, Stefan Zauscher, Shyni Varghese
Summary: Articular joints experience wear and tear throughout life, and synovial fluid acts as a natural lubricant to protect the cartilage. However, degenerative joint diseases can alter the composition of synovial fluid, leading to decreased protection. Scientists are developing next-generation viscosupplements, particularly those based on hyaluronic acid, to enhance the function of compromised synovial fluid. Non-hyaluronic acid-based viscosupplements with intricate molecular architectures, inspired by natural molecules in synovial fluid, also offer advantages over hyaluronic acid-based ones.
CURRENT OPINION IN COLLOID & INTERFACE SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Gavin Gonzales, Jiaul Hoque, Anna Gilpin, Biswanath Maity, Stefan Zauscher, Shyni Varghese
Summary: This study focuses on the development of cationic nanocarriers to overcome the challenges of drug delivery to cartilage tissue. These nanocarriers, which adhere quickly to cartilage tissue and can penetrate and remain within its full thickness, show promising results for therapeutic outcomes.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
