Article
Metallurgy & Metallurgical Engineering
Albert Saavedra, J. Viridiana Garcia-Meza, Eduardo Corton, Ignacio Gonzalez
Summary: The study presents a strategy for monitoring bacterial attachment to pyrite and surface modified pyrite using electrochemical impedance spectroscopy (EIS). Significant changes in adhesion process were observed at low frequencies, and the results obtained through EIS evaluation were consistent with traditional cell count method for bacterial attachment assessment.
Article
Chemistry, Multidisciplinary
Pirawish Limlawan, Nathaya Thepphanao, Numpon Insin, Anjalee Vacharaksa
Summary: Hydroxyapatite nanoparticles (HA-NPs), surface-modified with 3-aminopropyltriethoxysilane (HA-NPs-APTES) and poly2-(dimethylamino)ethyl methacrylate (HA-NPs-PDMAEMA), demonstrated optimal efficiency in miRNA delivery and gene regulation in osteoblasts. The HA-NPs-APTES particles showed the highest miRNA binding efficiency and internalization in human mandibular osteoblasts, while the PDMAEMA-modified particles showed potential cytotoxicity.
JOURNAL OF NANOPARTICLE RESEARCH
(2021)
Article
Materials Science, Ceramics
L. Stipniece, S. Wilson, J. M. Curran, R. Chen, K. Salma-Ancane, P. K. Sharma, B. J. Meenan, A. R. Boyd
Summary: This study synthesized hydroxyapatite and Sr-substituted hydroxyapatite powders with varying levels of Sr inclusion. It was found that higher Sr content in SrHAp directly promoted the maturation of osteoblasts into osteocytes compared to hydroxyapatite.
CERAMICS INTERNATIONAL
(2021)
Article
Engineering, Biomedical
F. Florian, F. P. S. Guastaldi, M. A. Cominotte, L. C. Pires, A. C. Guastaldi, J. A. Cirelli
Summary: The behavior of rat bone marrow stem cells on Ti-15Mo alloy surface modified by laser-beam irradiation and calcium phosphate deposition was evaluated. Laser treatment and phosphate deposition positively interacted with bone cells, showing differences in cell proliferation and biocompatibility.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jing Wang, Qingchen Qiao, Yaxi Sun, Wenting Yu, Jiran Wang, Minjia Zhu, Kai Yang, Xiaofeng Huang, Yuxing Bai
Summary: This study investigated the impact of cell culture density on stem cell proliferation, osteoblastogenesis, and regulation. The results showed that the proliferation rate of human periodontal ligament stem cells (hPDLSCs) decreased with an increase in initial plating density. After osteogenic differentiation, hPDLSCs initially plated at a density of 2 x 10(4) cells/cm(2) showed the highest expression of OPG and RUNX2, as well as the highest OPG/RANKL ratio and cell calcium concentration. Furthermore, exosomes derived from hPDLSCs cultured at different initial cell densities were found to regulate osteoblastic differentiation of human bone marrow stromal cells (hBMSC), with the 2 x 10(4) cells/cm(2) initial cell density group showing the highest expression of OPG, OCN, RUNX2, and osterix, as well as the highest average calcium concentration. This provides a new perspective for the clinical application of stem cell osteogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Natsumi Ibano, Emi Inada, Shinji Otake, Yuki Kiyokawa, Kensuke Sakata, Masahiro Sato, Naoko Kubota, Hirofumi Noguchi, Yoko Iwase, Tomoya Murakami, Tadashi Sawami, Yoshito Kakihara, Takeyasu Maeda, Miho Terunuma, Yutaka Terao, Issei Saitoh
Summary: This study aimed to elucidate the role of genetically modified human feeder cells expressing growth factors in maintaining the integrity of primary cultured human deciduous dental pulp cells. The results showed that co-culturing enhanced cell proliferation, promoted differentiation, and maintained stemness properties.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Physiology
Joana T. Rosa, Paul Eckhard Witten, Ann Huysseune
Summary: The translation discusses the relationship between osteoblasts and odontoblasts, as well as the diverse tooth attachment modes and tissue properties in mammals and teleost fish. There is a debate about the nature of the bone of attachment tissue and the cells secreting it, with scpp5 expression helping to characterize the cells involved in the process. The findings suggest that the bone of attachment in zebrafish shares characteristics of both bone and dentin, and may be better termed as dentinous bone.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Computer Science, Cybernetics
Pei-Yi (Patricia) Kuo, Li-Cheng Pan, Wen-Lin Chiao
Summary: This study presents the design and evaluation of the cAMpanion ambient display, which shows the status of pet dogs in real time to enhance the sense of connectedness between distant pet owners and their dogs. The results from a 14-day field study showed that cAMpanion raised awareness, decreased negative emotions, and increased perceived closeness in pet owners.
INTERNATIONAL JOURNAL OF HUMAN-COMPUTER INTERACTION
(2023)
Article
Engineering, Biomedical
Paritat Thaitalay, Oranich Thongsri, Rawee Dangviriyakul, Sawitri Srisuwan, Louise Carney, Julie Elizabeth Gough, Sirirat Tubsungnoen Rattanachan
Summary: In this study, three different modified cements were evaluated for their physical properties and the response of primary human osteoblast cells and mesenchymal stem cells. Polyacrylic acid was found to improve the compressive strength and Young's modulus of the cement, but it had negative effects on apatite phase formation, setting time, and degradation. The addition of bioactive glass improved the physical properties of the cement and enhanced cell proliferation and differentiation, making it a promising formula for bone repair applications.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Chemistry, Multidisciplinary
S. W. M. A. Ishantha Senevirathne, Yi-Chin Toh, Prasad K. D. Yarlagadda
Summary: Nanotopographic surfaces have been proven to effectively kill bacterial cells upon contact, providing an alternative method to fight against bacterial colonization and resistance. This study investigates how fluid flow affects bacterial cell adherence on nanotopographic surfaces, revealing that increasing flow rates significantly reduce the number of live cells, while increasing the number of dead cells adhering to the surface.
Article
Cell Biology
Noreen Mumtaz, Amel Dudakovic, Asha Nair, Marijke Koedam, Johannes P. T. M. van Leeuwen, Marion P. G. Koopmans, Barry Rockx, Andre J. J. van Wijnen, Bram C. J. van der Eerden
Summary: Arboviruses like Zika virus affect bone development by targeting osteoblasts and altering bone remodeling. In this study, the researchers investigated the effects of Zika virus infection on mesenchymal stromal cells (MSCs) at different stages of osteogenesis. They found that late-stage infected MSCs showed elevated osteogenic markers, calcium content, and altered gene expression compared to early-stage infected MSCs.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Chemistry, Analytical
Bailey C. Buchanan, Babak Safavinia, Lillian Wu, Jeong-Yeol Yoon
Summary: This study developed a method to detect bacteria on laboratory surfaces using low-cost components and a smartphone, without the need for culturing or staining. The presence of Escherichia coli, Salmonella Typhimurium, and Staphylococcus aureus was successfully detected, and the method could also distinguish from tap water, protein, and NaCl solutions.
Article
Biochemical Research Methods
Harlei Martin, David Goyard, Anatte Margalit, Kyle Doherty, Olivier Renaudet, Kevin Kavanagh, Trinidad Velasco-Torrijos
Summary: The study found that a specific divalent galactoside glycomimetic 1 has inhibitory effects on the adhesion and biofilm formation of Candida albicans. The multivalent presentations of glycomimetic 1 showed varying effectiveness in inhibiting yeast adhesion and biofilm formation, with the core scaffold significantly influencing the activity of lower valency conjugates.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Xiaohui Zhang, Ting Zhang, Bin Liu, Yun Zhang, Zhongying Ji, Xiaolong Wang
Summary: The study demonstrates that biomimetic micropatterned surfaces from natural leaves significantly regulate the morphology and adhesion behavior of cervical cancer cells.
Article
Biochemistry & Molecular Biology
Sandra Pihlstrom, Kirsi Maatta, Tiina Ohman, Riikka E. Makitie, Mira Aronen, Markku Varjosalo, Outi Makitie, Minna Pekkinen
Summary: This study developed an in vitro culturing technique to transdifferentiate fibroblasts into osteoblast-like cells, and confirmed the osteoblastic phenotype through various staining and analysis methods. This culturing system provides an alternative tool for bone biology research and skeletal tissue engineering.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)