Quantitative proteomics using SILAC: Principles, applications, and developments
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Title
Quantitative proteomics using SILAC: Principles, applications, and developments
Authors
Keywords
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Journal
PROTEOMICS
Volume 15, Issue 18, Pages 3175-3192
Publisher
Wiley
Online
2015-06-20
DOI
10.1002/pmic.201500108
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- (2014) Ho-Tak Lau et al. JOURNAL OF PROTEOME RESEARCH
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- (2014) Katrin Eichelbaum et al. MOLECULAR & CELLULAR PROTEOMICS
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- (2014) Wen Zhang et al. PROTEOMICS
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- (2014) Xinyan Wu et al. Nature Communications
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- (2014) Xiulan Chen et al. Theranostics
- Acetylation dynamics of human nuclear proteins during the ionizing radiation-induced DNA damage response
- (2013) Martin Bennetzen et al. CELL CYCLE
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- (2013) Sean J. Humphrey et al. Cell Metabolism
- Evaluation of Drosophila Metabolic Labeling Strategies for in Vivo Quantitative Proteomic Analyses with Applications to Early Pupa Formation and Amino Acid Starvation
- (2013) Ying-Che Chang et al. JOURNAL OF PROTEOME RESEARCH
- SAHA Treatment Reveals the Link between Histone Lysine Acetylation and Proteome in Nonsmall Cell Lung Cancer A549 Cells
- (2013) Quan Wu et al. JOURNAL OF PROTEOME RESEARCH
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- (2013) Devin K. Schweppe et al. Journal of Proteomics
- Stable Isotope Labeling in Zebrafish Allowsin VivoMonitoring of Cardiac Morphogenesis
- (2013) Anne Konzer et al. MOLECULAR & CELLULAR PROTEOMICS
- The Coming of Age of Phosphoproteomics—from Large Data Sets to Inference of Protein Functions
- (2013) Philippe P. Roux et al. MOLECULAR & CELLULAR PROTEOMICS
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- (2013) Sree Rayavarapu et al. MOLECULAR & CELLULAR PROTEOMICS
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- (2013) F. Meissner et al. SCIENCE
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- (2012) Luisa Beltran et al. AMINO ACIDS
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- (2012) Shao-En Ong ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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