Article
Microbiology
Min Ying, Huadong Wang, Tongtan Liu, Zengpeng Han, Kunzhang Lin, Qing Shi, Ning Zheng, Tao Ye, Huinan Gong, Fuqiang Xu
Summary: Herpes simplex virus type 1 (HSV-1) is a major cause of encephalitis and infectious blindness. The current drugs for HSV cannot eliminate the latent virus or viral reactivation, hence the need for new treatment strategies. The CLEAR strategy, targeting crucial genes involved in HSV infection lifecycle, effectively inhibits HSV replication. The combination of CRISPR-Cas9 editing and cocktail administration method shows the most promising results in reducing viral proliferation.
Article
Pharmacology & Pharmacy
Hiroki Kondo, Tetsuo Koshizuka, Ryuichi Majima, Keita Takahashi, Ken Ishioka, Tatsuo Suzutani, Naoki Inoue
Summary: The compound 147B3 has shown efficacy in inhibiting HCMV and HSV-1 infection, potentially by targeting viral transactivators through their interaction with factors required for the viral gene expression system.
ANTIVIRAL RESEARCH
(2021)
Article
Microbiology
Fujun Hou, Zeyu Sun, Yue Deng, Siyu Chen, Xiyuan Yang, Feiyang Ji, Menghao Zhou, Keyi Ren, Dongli Pan
Summary: This study investigates the targets of ICP0 in HSV-1 infection and its roles in neuronal cells. Mass spectrometry analysis identified various proteins interacting with ICP0 in Neuro-2a and 293T cells. Further experiments confirmed that SNX9 and OTUD4 restrict the replication of ICP0-null virus in neuronal cells, and OTUD4 enhances the expression of type I interferon during infection with ICP0-null virus.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fanghui Ren, Ryo Narita, Ahmad S. Rashidi, Stefanie Fruhwurth, Zongliang Gao, Rasmus O. Bak, Martin K. Thomsen, Georges M. G. M. Verjans, Line S. Reinert, Soren R. Paludan
Summary: Neurotropic viruses such as herpes simplex virus (HSV) can infect neurons and cause severe diseases. HSV-induced neuronal cell death is mediated by gasdermin E (GSDME) and involves endoplasmic reticulum stress, caspase activation, and mitochondria-dependent apoptosis. The necrotic neurons release alarmins, triggering inflammatory responses in microglia.
Article
Immunology
Jingjing Zhang, Xingli Xu, Suqin Duan, Yang Gao, Danjing Ma, Rong Yue, Fengyuan Zeng, Xueqi Li, Ziyan Meng, Xinghang Li, Zhenye Niu, Guorun Jiang, Li Yu, Yun Liao, Dandan Li, Lichun Wang, Heng Zhao, Ying Zhang, Qihan Li
Summary: This study revealed the strategy of how HSV-1 infection of dendritic cells interferes with the immune system, by investigating the impact of virus infection on both innate and adaptive immunity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Virology
Beibei Zhang, Juntao Ding, Zhenghai Ma
Summary: It has been demonstrated that the HSV-1 IE gene ICP4 inhibits PKA phosphorylation to induce Rap1b-activation-mediated viral infection. Rap1b activation and membrane enrichment occur early in HSV-1 infection and remain active during viral proliferation. Inhibition of Rap1b activation derives from phosphorylated PKA and Rap1b mutants with prenylation, promoting viral infection in a dose-dependent manner.
Article
Ophthalmology
Pratima Krishna Suvas, Mizumi Setia, Mashidur Rana, Anish Chakraborty, Susmit Suvas
Summary: This study aimed to characterize CXCR4-expressing cells in uninfected and herpes simplex virus-1 (HSV-1) infected corneas. The expression of CXCR4 and CXCL12 transcripts in uninfected and HSV-1-infected corneas was detected using RT-qPCR assay. Immunofluorescence staining and flow cytometry assay were used to characterize the CXCR4-expressing cells in the corneas.
Article
Clinical Neurology
Diana N. Tran, Amy T. C. M. Bakx, Vera Dis, Eleonora Aronica, Robert M. Verdijk, Werner J. D. Ouwendijk
Summary: The findings suggest that latent or lytic HSV infection in human neurons is not directly associated with aberrant A beta or pTau protein expression. Some aging-related abnormalities were observed in the brains of HSE patients, but they were not limited to HSV-infected cells or brain regions.
Article
Biology
Ilaria Frasson, Paola Solda, Matteo Nadai, Sara Lago, Sara N. Richter
Summary: Researchers found that the herpesvirus major transcription factor ICP4 regulates viral gene expression and replication by binding to parallel DNA G-quadruplexes (G4s). These results suggest that drugs targeting G4s at ICP4-regulated gene promoters could be an effective antiviral strategy.
COMMUNICATIONS BIOLOGY
(2021)
Article
Infectious Diseases
Sarah H. Brown, Vanessa A. R. States, Abaseen K. Afghan, Gowri Satyanarayana
Summary: This is a case of a 65-year-old female patient with a history of acute myelogenous leukemia and buccal squamous cell carcinoma, who underwent peripheral blood stem cell transplant and was found to have HSV-1-infected cancer cells on histopathological examination and was treated with valacyclovir.
BMC INFECTIOUS DISEASES
(2022)
Article
Pathology
Melissa Krystel-Whittemore, May P. Chan, Sara C. Shalin, Kenan J. Sauder, Amy Hudson, Ruth K. Foreman, Mai P. Hoang, Jeoffry B. Brennick, Shaofeng Yan, Rosalynn M. Nazarian
Summary: This study presents the first known report of herpes virus infecting deep stromal cells of the dermis, highlighting the importance of considering cutaneous stromal herpes in patients with atypical clinical lesions, especially when immunocompromised. Establishing the correct diagnosis is crucial for initiating therapy.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Immunology
Christopher M. Pruitt, Lise E. Nigrovic, Stephen B. Freedman, Stuart A. Bradin, Sarah J. Curtis, Todd W. Lyons, Aaron S. Miller, Pamela J. Okada, Suzanne M. Schmidt, David Schnadower, Joanna E. Thomson, Andrea T. Cruz
Summary: Despite clear testing recommendations, there is a lack of comprehensive HSV testing in infants. A study of 112 infants with confirmed HSV disease from 23 research centers found that less than one-fifth of the infants underwent all recommended testing, emphasizing the need for increased awareness and adherence to testing recommendations for this vulnerable population.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2022)
Article
Dermatology
Deepthi Konda, Laxmisha Chandrashekar, Rahul Dhodapkar, Rajesh Nachiappa Ganesh, Devinder Mohan Thappa
Summary: This study characterized the clinical markers of herpes simplex virus (HSV) infection among patients with pemphigus vulgaris. It was found that male sex, presence of fissures, hemorrhagic crusts, erosions with angulated margins, linear erosions, and raised erythrocyte sedimentation rate were significantly associated with HSV infection. Hemorrhagic crusts and linear erosions were identified as independent predictors of HSV infection.
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2023)
Article
Immunology
Malgorzata Krzyzowska, Anders Jarneborn, Karolina Thorn, Kristina Eriksson, Tao Jin
Summary: This study provides evidence that treatment with tofacitinib may increase the risk of disease aggravation and severe encephalitis in primary herpes simplex infection by impairing antiviral response induced by monocytes and microglia.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Cooper K. Hayes, Christopher K. Villota, Fiona B. McEnany, Stacey Ceron, Sita Awasthi, Moriah L. Szpara, Harvey M. Friedman, David A. Leib, Richard Longnecker, Matthew D. Weitzman, Lisa N. Akhtar
Summary: Clinical HSV-2 isolates collected from neonates with encephalitis are more neurovirulent in human neuronal cell culture and murine models as compared to isolates from neonates with skin-limited disease, suggesting that viral factors contribute to neurologic outcome following human neonatal infection.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Hyung Suk Oh, Paula Traktman, David M. Knipe
Article
Cell Biology
David Dulin, Jamie J. Arnold, Theo van Laar, Hyung-Suk Oh, Cheri Lee, Angela L. Perkins, Daniel A. Harki, Martin Depken, Craig E. Cameron, Nynke H. Dekker
Article
Microbiology
Hyung S. Oh, Sravani Banerjee, David Aponte-Diaz, Suresh D. Sharma, Jason Aligo, Maria F. Lodeiro, Gang Ning, Rajni Sharma, Jamie J. Arnold, Craig E. Cameron
Article
Microbiology
Sravani Banerjee, David Aponte-Diaz, Calvin Yeager, Suresh D. Sharma, Gang Ning, Hyung S. Oh, Qingxia Han, Masato Umeda, Yuji Hara, Robert Y. L. Wang, Craig E. Cameron
Article
Virology
Meg Trahey, Hyung Suk Oh, Craig E. Cameron, Jesse C. Hay
JOURNAL OF VIROLOGY
(2012)
Article
Chemistry, Multidisciplinary
Alyssa M. Larson, Hyung Suk Oh, David M. Knipe, Alexander M. Klibanov
PHARMACEUTICAL RESEARCH
(2013)
Article
Microbiology
Hyung Suk Oh, Kevin F. Bryant, Thomas J. F. Nieland, Aprotim Mazumder, Mukta Bagul, Mark Bathe, David E. Root, David M. Knipe
Article
Biology
Joseph M. Cabral, Hyung Suk Oh, David M. Knipe
Article
Biochemistry & Molecular Biology
Alyson K. Boehr, Jamie J. Arnold, Hyung S. Oh, Craig E. Cameron, David D. Boehr
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biology
Hyung Suk Oh, Werner M. Neuhausser, Pierce Eggan, Magdalena Angelova, Rory Kirchner, Kevin C. Eggan, David M. Knipe
Article
Microbiology
Boqiang Sun, Xuewei Yang, Fujun Hou, Xiaofeng Yu, Qiongyan Wang, Hyung Suk Oh, Priya Raja, Jean M. Pesola, Emilia A. H. Vanni, Seamus McCarron, Jenna Morris-Love, Alex H. M. Ng, George M. Church, David M. Knipe, Donald M. Coen, Dongli Pan
Summary: MicroRNA miR-138 targets ICP0, Oct-1, and Foxc1 to repress HSV-1 lytic cycle genes and promote epigenetic gene silencing, favoring viral latency in neuronal cells. Integration of genome-wide RNA sequencing/PAR-CLIP and short interfering RNA knockdown techniques reveals the mechanism by which miR-138 regulates HSV-1 replication and latency.
NATURE MICROBIOLOGY
(2021)
Article
Virology
Evelyn A. Kurt-Jones, Timothy E. Dudek, Daisuke Watanabe, Leisa Mandell, Jenny Che, Shenghua Zhou, LuCheng Cao, Thomas Greenough, Gregory J. Babcock, Fernando Diaz, Hyung Suk Oh, Changhong Zhou, Robert W. Finberg, David M. Knipe
Summary: This study constructed a replication-defective mutant vector expressing SARS-CoV-1 spike protein, inducing the production of antibodies with binding and neutralizing activity in immunized mice. The spike protein can independently induce cytokine expression through MyD88, contributing directly to the inflammatory process of SARS.
Article
Multidisciplinary Sciences
Juliette Leon, Daniel A. Michelson, Judith Olejnik, Kaitavjeet Chowdhary, Hyung Suk Oh, Adam J. Hume, Silvia Galvan-Pena, Yangyang Zhu, Felicia Chen, Brinda Vijaykumar, Liang Yang, Elena Crestani, Lael M. Yonker, David M. Knipe, Elke Muhlberger, Christophe Benoist
Summary: Through in vitro co-culture modeling, the study found that the interaction between infected epithelial cells and immunocytes in SARS-CoV-2 infection leads to immune dysregulation, which may be the root cause of the inflammatory malfunction in COVID-19.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Werner M. Neuhausser, Hyung S. Oh, Pierce Eggan, Magdalena Angelova, Rory Kirchner, Kevin C. Eggan, David M. Knipe