Mutations in adenine-binding pockets enhance catalytic properties of NAD(P)H-dependent enzymes

Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases

(2015) Jackson K.B. Cahn et al.   BIOCHEMICAL JOURNAL

Identification and optimization of a novel thermo- and solvent stable ketol-acid reductoisomerase for cell free isobutanol biosynthesis

(2015) Steven Reiße et al.   BIOCHIMIE

Recent advances in engineering proteins for biocatalysis

(2014) Ye Li et al.   BIOTECHNOLOGY AND BIOENGINEERING

Structure ofEscherichia coliAdhP (ethanol-inducible dehydrogenase) with bound NAD

(2013) Leonard M. Thomas et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH

(2013) S. Brinkmann-Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stability-mediated epistasis constrains the evolution of an influenza protein

(2013) Lizhi Ian Gong et al.   eLife

Engineering specialized metabolic pathways—is there a room for enzyme improvements?

(2012) Arren Bar-Even et al.   CURRENT OPINION IN BIOTECHNOLOGY

Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to isobutanol

(2012) Xiang Liu et al.   JOURNAL OF BIOTECHNOLOGY

Bacterial and Plant Ketol-Acid Reductoisomerases Have Different Mechanisms of Induced Fit during the Catalytic Cycle

(2012) Sze-Ho Wong et al.   JOURNAL OF MOLECULAR BIOLOGY

Evolutionary layering and the limits to cellular perfection

(2012) M. Lynch   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Drift-barrier hypothesis and mutation-rate evolution

(2012) W. Sung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Modular exchange of substrate-binding loops alters both substrate and cofactor specificity in a member of the aldo-keto reductase superfamily

(2012) E. Campbell et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme

(2012) Nobuhiko Tokuriki et al.   Nature Communications

Increased Furfural Tolerance Due to Overexpression of NADH-Dependent Oxidoreductase FucO in Escherichia coli Strains Engineered for the Production of Ethanol and Lactate

(2011) X. Wang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The Moderately Efficient Enzyme: Evolutionary and Physicochemical Trends Shaping Enzyme Parameters

(2011) Arren Bar-Even et al.   BIOCHEMISTRY

Structures of Iron-Dependent Alcohol Dehydrogenase 2 from Zymomonas mobilis ZM4 with and without NAD+ Cofactor

(2011) Ji-Hyun Moon et al.   JOURNAL OF MOLECULAR BIOLOGY

Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli

(2011) Sabine Bastian et al.   METABOLIC ENGINEERING

XDS

(2010) Wolfgang Kabsch   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Optimizing non-natural protein function with directed evolution

(2010) Eric M Brustad et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Cross-species analysis traces adaptation of Rubisco toward optimality in a low-dimensional landscape

(2010) Yonatan Savir et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conformational changes and catalysis by alcohol dehydrogenase

(2009) Bryce V. Plapp   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli

(2009) Bryson D Bennett et al.   Nature Chemical Biology

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Computational design ofCandida boidiniixylose reductase for altered cofactor specificity

(2009) George A. Khoury et al.   PROTEIN SCIENCE

1,3-Propanediol Dehydrogenase from Klebsiella pneumoniae: Decameric Quaternary Structure and Possible Subunit Cooperativity

(2008) D. Marcal et al.   JOURNAL OF BACTERIOLOGY

Evolutionary History of a Specialized P450 Propane Monooxygenase

(2008) Rudi Fasan et al.   JOURNAL OF MOLECULAR BIOLOGY