Article
Biotechnology & Applied Microbiology
Kristiana Kovtuna, Inese Strazdina, Mara Bikerniece, Nina Galinina, Reinis Rutkis, Jekaterina Martynova, Uldis Kalnenieks
Summary: The resistance to oxidative stress was studied in mutant strains of Zymomonas mobilis with modified central metabolism. It was found that the mutations improved H2O2 resistance and catalase activity, while the overexpression of Ndh had the opposite effect. Strains without catalase were unable to grow, and their resistance was not dependent on the expression levels of AdhB or Ndh.
FERMENTATION-BASEL
(2022)
Review
Food Science & Technology
Lu Liu, Jian-Ting Li, Sheng-Hao Li, Lin-Pei Liu, Bo Wu, Yan-Wei Wang, Shi-Hui Yang, Cheng-Han Chen, Fu-Rong Tan, Ming-Xiong He
Summary: This review summarizes the physiological characteristics and advantages of Z. mobilis in the food industry for the first time, and further elaborates its research status in the food industry in terms of food additive synthesis, fermentation applications, and prebiotic efficacy, providing a theoretical basis for its development and application in the food industry. The review also discusses the shortcomings of its practical applications in the current food industry and explores other ways to expand the applications of Z. mobilis in food processing.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Chemistry, Multidisciplinary
Victor. K. K. Sharma, Kylee. J. J. Cosse, Thomas. P. P. Binder, Jeffrey. S. S. McFarlane, Alan. M. M. Allgeier
Summary: In this study, furfuryl alcohol (FOL) was biocatalytically produced from biomass-derived furfural (FAL) using ethanol (EtOH) as the terminal reductant, employing NAD(P)H-dependent yeast alcohol dehydrogenase I (YADH) as the catalyst. The conversion of FAL to FOL reached up to 74% under pH 8 with initial FAL and EtOH concentrations of 40 mM and 160 mM, respectively. This research lays the foundation for developing alternative processes to biomass-derived ethanol.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Microbiology
Christiane Ribeiro Janner Herrera, Vanessa Rodrigues Vieira, Tiago Benoliel, Clara Vida Galrao Correa Carneiro, Janice Lisboa De Marco, Lidia Maria Pepe de Moraes, Joao Ricardo Moreira de Almeida, Fernando Araripe Goncalves Torres
Summary: Sugarcane bagasse, a rich source of xylose, was utilized to produce xylonic acid with engineered Zymomonas mobilis. Expression of XDH gene from Paraburkholderia xenovorans led to the highest production, with deletion of xylose reductase gene further increasing yields. The findings suggest Z. mobilis could serve as a potential platform for organic acid production from cheap lignocellulosic biomass in biorefineries.
Article
Biotechnology & Applied Microbiology
Junyi Hu, William Wang, Feifei Zhang, Xuequan Jiang, Lida Peng, Yichao Fang, Haoyong Wang
Summary: This study presents a metabolic engineering strategy of Zymomonas mobilis aimed at coproducing bioethanol and fatty acids. The recombinant strain achieved higher ethanol and fatty acid production compared to the wild-type strain in glucose medium. It also showed potential for sustainable production of ethanol and fatty acids from lignocellulosic renewable carbon sources.
FERMENTATION-BASEL
(2023)
Article
Agricultural Engineering
Yuqi Li, Yao Xu, Yihan Xue, Shihui Yang, Yanfen Cheng, Weiyun Zhu
Summary: A novel integrated process for ethanol production from lignocellulosic biomass is reported. The process involves simultaneous pretreatment, saccharification, and fermentation using environmentally friendly technologies. The conversion from lignocellulosic biomass to ethanol was completed in four days with the potential for producing other metabolites.
BIOMASS & BIOENERGY
(2022)
Article
Food Science & Technology
S. H. Patel, J. P. Tan, R. A. Borner, S. J. Zhang, S. Priour, A. Lima, C. Ngom-Bru, P. D. Cotter, S. Duboux
Summary: Through metagenomics, chemical profiling, and sensory evaluation, the dynamic nature of water kefir fermentation and the contribution of different microbial members to flavor have been elucidated. This research not only enhances our understanding of water kefir microbiology, but also provides a foundation for the development of defined starter cultures that can reproduce the main attributes of traditionally brewed water kefir.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2022)
Article
Biochemistry & Molecular Biology
Joalene de Azevedo Santos Ferreira, Igor Carvalho Fontes Sampaio, Carolaine Ellen da Cruz Hora, Josilene Borges Torres Lima Matos, Paulo Fernando de Almeida, Fabio Alexandre Chinalia
Summary: This study tested three different media as supplementation for produced water in order to produce levan. The research found that high sucrose concentrations combined with ammonium and magnesium sulfates can mitigate the negative effect of high salt concentrations on levan production. Using supplemented produced water as medium, 10 g/L of levan could be produced within 24 hours. The highest production of 31.9 g/L was obtained with dialyzed pretreated produced water. The produced levan showed no significant differences compared to the commercial counterpart according to FT-IR characterization.
PROCESS BIOCHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Nosaibeh Nosrati-Ghods, Susan T. L. Harrison, Adeniyi J. Isafiade, Siew Leng Tai
Summary: This study investigated the relationship between wild type and engineered strains of the same microorganism in terms of kinetic parameters, and proposed a set of correction factors to estimate kinetic constant values for species or strains for which they are not available. The results showed a correlation between wild type and engineered strains, with specific substrate limitation constants being higher in the engineered strains. These correction factors were found to be conserved and could serve as a guide for estimating kinetic constants in other species or strains.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Lian-Ying Cao, Chen-Guang Liu, Shi-Hui Yang, Feng-Wu Bai
Summary: In this study, a novel strategy was developed to enhance the stress tolerance of Z. mobilis by regulating biofilm formation through heterologous expression of pfs and luxS genes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kun Zhang, Wenwen Zhang, Mengxing Qin, Yi Li, Hailei Wang
Summary: This study investigates the role of ZMO0293 in Z. mobilis using gene deletion and heterologous expression methods. The results show that deletion of ZMO0293 gene leads to slowed growth, reduced ethanol production, and decreased activities of key enzymes involved in glucose metabolism in the presence of high concentrations of glucose. Furthermore, ZMO0293 deletion causes transcriptional changes in some genes of the Entner Doudoroff pathway in the ZM4-Delta ZM0293 strain. This study reveals the function of ZMO0293 gene and provides a new biological part for synthetic biology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Dongsheng Hu, Zhiquan Wang, Mingxiong He, Yuanyuan Ma
Summary: The key tolerance mechanism of a mutant Zymomonas mobilis strain F211 with high furfural tolerance was studied in this research. Functional complementation experiments and comparative transcriptome analysis revealed the genes responsible for improved furfural tolerance in F211, providing insights into the potential tolerance mechanism and corresponding functional genes for developing robust chassis strains for synthetic biology efforts.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biology
Reinis Rutkis, Inese Strazdina, Zane Lasa, Per Bruheim, Uldis Kalnenieks
Summary: A mutant strain with partly inactivated Pdc showed increased pyruvate production compared to its parent strain, along with increased acetaldehyde production despite similar ADH activity. Simulation with the kinetic model of Z. mobilis E-D pathway suggested a higher ratio between respiratory NADH oxidase and ADH activities is needed for the observed acetaldehyde to ethanol production ratio in the mutant. This finding has implications for the directionality of ADH isoenzyme operation in vivo and interactions between ADH and Pdc.
BMC RESEARCH NOTES
(2021)
Article
Biotechnology & Applied Microbiology
Samina Shabbir, Weiting Wang, Mohsin Nawaz, Prerona Boruah, Muhammad Fakhar-e-Alam Kulyar, Mao Chen, Bo Wu, Panting Liu, Yonghua Dai, Lingling Sun, Qiyu Gou, Renbin Liu, Guoquan Hu, Tahira Younis, Mingxiong He
Summary: This study investigated the response of Zymomonas mobilis 532 (ZM532) and its wild-type Z. mobilis (ZM4) to acetic acid and furfural (AF) through multi-omics approaches. The results revealed differentially expressed genes and proteins in ZM532 and ZM4, with ZMO_RS02740 identified as an important gene in ZM532's growth and glucose consumption.
MICROBIAL CELL FACTORIES
(2023)
Article
Microbiology
Manuela Maragno do Almo, Isabel Garcia Sousa, Vitor Guimaraes Olinto, Sylvia Barbosa Pinhate, Jose Luiz de Paula Rolo Jivago, Davi Emanuel Ribeiro de Sousa, Marcio Botelho de Castro, Marciano Regis Rubini, Andrea Queiroz Maranhao, Marcelo Macedo Brigido
Summary: In this study, the effects of Zymomonas mobilis on an experimental colitis model in mice were investigated. The findings suggest that Z. mobilis could alleviate the symptoms, restore the mucosal barrier, alter gut microbiota, and induce immunomodulation, making it a potential probiotic adjuvant for pathologies of the bowel.
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)