Article
Biochemistry & Molecular Biology
Dariusz Zakrzewicz, Joachim Geyer
Summary: Hepatitis B virus infections are a major public health concern worldwide. A recently discovered high-affinity hepatic host receptor has become a key focus of research for potential therapies. This article presents a newly generated three-dimensional model of the receptor and discusses its role in the entry of HBV into hepatocytes.
Article
Medicine, Research & Experimental
Takuro Uchida, Seung Bum Park, Tadashi Inuzuka, Min Zhang, Joselyn N. Allen, Kazuaki Chayama, T. Jake Liang
Summary: The NTCP-S267F variant, associated with reduced risk of HBV infection, was introduced into hepatic cells via CRISPR base editing. Homozygous clones did not support HBV infection, while heterozygous clones behaved similarly to wild-type. This study provides valuable insights for therapeutic intervention against HBV.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Cell Biology
Zongmei Zheng, Jiao Wei, Xinxin Hou, Fengjing Jia, Zhaozhou Zhang, Haidong Guo, Fuwen Yuan, Feng He, Zunji Ke, Yan Wang, Ling Zhao
Summary: This study reveals a new bile acid-driven mechanism of colorectal cancer-associated liver metastasis. The accumulation of taurocholic acid (TCA) in the liver promotes liver metastasis and creates a tumor-favorable microenvironment. Blocking hepatic bile acid uptake effectively suppresses TCA-induced liver metastasis.
Article
Biophysics
Marco Fornasier, Francesca Dessi, Rosa Pireddu, Chiara Sinico, Emiliano Carretti, Sergio Murgia
Summary: The encapsulation of natural antioxidants into lipid vesicular gels made by monoolein and sodium taurocholate was studied for topical application. Bile salts were found to affect the phase behavior of monoolein and form suitable drug carriers. Oscillating rheological studies showed that ferulic acid has a structuring effect on the lipid matrix. In vitro experiments on pig skin demonstrated that caffeic acid is mostly retained in the gel while ferulic acid is released to a higher degree.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Review
Gastroenterology & Hepatology
Roni F. Kunst, Henkjan J. Verkade, Ronald P. J. Oude Elferink, Stan F. J. van de Graaf
Summary: Bile salts are essential for lipid homeostasis and can be both culprits and potential remedies in gut or liver disorders. Efficient transporters mediate the uptake and efflux of bile salts, ensuring their enterohepatic circulation. Deficiencies in key transporters can lead to pathological conditions, and targeting these pathways may offer new therapeutic opportunities.
Article
Pharmacology & Pharmacy
Dongliang Cheng, Zhiwei Wen, Hui Chen, Shiyuan Lin, Wei Zhang, Xin Tang, Wei Wu
Summary: In this study, a functionalized liposome PPP-LIP was modified to have tumor targetability and enhanced internalization after MMP2-triggered TATp exposure for liver tumor-specific delivery. The results showed that PPP-LIP was taken up more by tumor cells and had a better antitumor effect compared to commercially available injections.
Review
Pediatrics
Ting-Ting Zou, Yu Zhu, Chao-Min Wan, Qiong Liao
Summary: Sodium-taurocholate cotransporting polypeptide (NTCP) deficiency is a newly reported hereditary bile acid metabolic disease, presenting most commonly with jaundice in pediatric patients. It can be detected during routine check-ups and is characterized by hypercholanemia and elevated AST. Screening for c.800C>T mutation in SLC10A1 is useful for primary genetic screening in Chinese infants with persistent hypercholanemia after infectious, structural, and immunological factors are excluded.
TRANSLATIONAL PEDIATRICS
(2021)
Article
Pharmacology & Pharmacy
Yueping Zhang, Shen-Jue Chen, Cliff Chen, Xue-Qing Chen, Sagnik Chatterjee, David J. Shuster, Heather Dexter, Laura Armstrong, Elizabeth M. Joshi, Zheng Yang, Hong Shen
Summary: This study demonstrates that CDCA suppresses the expression of OATP1B1 and OATP1B3 in monkeys, which may have implications for bile acid homeostasis and potential drug interactions.
DRUG METABOLISM AND DISPOSITION
(2022)
Article
Endocrinology & Metabolism
Fangji Yang, Wenxiong Xu, Lina Wu, Luo Yang, Shu Zhu, Lu Wang, Wenbin Wu, Yuzhen Zhang, Yutian Chong, Liang Peng
Summary: The p.Ser267Phe mutation in the SLC10A1 gene can cause NTCP deficiency, leading to hypercholanemia, vitamin D deficiency, bone loss, and gallbladder abnormalities in adult individuals. NTCP deficiency is also associated with elevated levels of serum bile acids and decreased levels of vitamin D, as well as aggravated osteoporotic phenotype.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Microbiology
Yingying Li, Guangxiang Luo
Summary: HBV infection is a major global health issue, with current treatments being limited. Recent research has shown that LDLR plays a crucial role in HBV infection, suggesting potential new avenues for HBV therapy. Further studies are needed to explore the involvement of other LDLR family members in HBV infection.
Article
Biochemical Research Methods
Qisong Zhang, Zhuoru He, Zhongqiu Liu, Lingzhi Gong
Summary: This study utilized a knockout mouse model to investigate the physiological functions of NTCP, revealing that its deficiency leads to significant metabolic changes in the plasma and liver of mice. Metabolomics analysis identified numerous differential metabolites in the liver and plasma, with dysregulation in multiple metabolic pathways. The findings suggest potential diagnostic biomarkers and therapeutic strategies for NTCP deficiency.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Ji Song, Guomin Ou, Xinyuan Lai, Xing Tian, Luwei Wang, Kai Zhang, Lei Wang, Xuechao Zhang, Chengyu Zhao, Hui Zhuang, Kuanhui Xiang, Tong Li
Summary: The study found that Matrigel (R) hESC-qualified (M-hq) enhances the infection efficiency of HBV in vitro, without affecting cell viability, HBV transcription, or response to antiviral treatments. The enhancement of infection is reproducible and is suggested to occur at the HBV attachment step. This novel system is recommended for studying HBV biology and new drugs.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Obstetrics & Gynecology
Kader Irak, Mehmet Bayram, Sami Cifci, Zuat Acar, Cemal Kazezoglu, Elif Yorulmaz, Suleyman Yildirim
Summary: This study evaluated the levels of OST-alpha, OST-beta, and NTCP in patients with ICP, finding differences between ICP patients and healthy controls. OST-alpha and OST-beta levels can be used as diagnostic and monitoring markers for ICP, and inhibiting these molecules may provide therapeutic benefits for ICP patients.
GINEKOLOGIA POLSKA
(2021)
Article
Chemistry, Multidisciplinary
Stanislav Kler, Ran Zalk, Alexander Upcher, Idit Kopatz
Summary: This article reports a new type of nanoparticle with a capsid composed of three SV40 virus capsid proteins, which closely mimics the intracellular pathway of the virus and has potential applications in gene delivery.
Article
Virology
Yingying Song, Shuyu Shou, Huimin Guo, Zixiang Gao, Nannan Liu, Yang Yang, Feifei Wang, Qiang Deng, Jing Liu, Youhua Xie
Summary: In this study, BEL7404 cell line displayed efficient DNA transfection and high levels of HBV antigen expression without prominent viral replication after transfection of HBV replicons. By introducing exogenous HNF4α and hNTCP expression, BEL7404-derived cell lines with induced HBV permissiveness and susceptibility were successfully constructed, providing a new tool for studying HBV replication and infection as well as antiviral drug characterization.
Article
Biochemistry & Molecular Biology
Fengfeng Mao, Teng Liu, Xinfeng Hou, Hanqing Zhao, Wenhui He, Cong Li, Zhiyi Jing, Jianhua Sui, Fengchao Wang, Xiaohui Liu, Jun Han, Christoph H. Borchers, Jian-She Wang, Wenhui Li
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Gastroenterology & Hepatology
Peter A. Revill, Francis V. Chisari, Joan M. Block, Maura Dandri, Adam J. Gehring, Haitao Guo, Jianming Hu, Anna Kramvis, Pietro Lampertico, Harry L. A. Janssen, Massimo Levrero, Wenhui Li, T. Jake Liang, Seng-Gee Lim, Fengmin Lu, M. Capucine Penicaud, John E. Tavis, Robert Thimme, Fabien Zoulim
LANCET GASTROENTEROLOGY & HEPATOLOGY
(2019)
Article
Pharmacology & Pharmacy
Zhenchao Gao, Liwei Yan, Wenhui Li
ANTIVIRAL RESEARCH
(2019)
Article
Microbiology
Huan Yan, Suan-Sin Foo, Weiqiang Chen, Ji-Seung Yoo, Woo-Jin Shin, Christine Wu, Jae U. Jung
Article
Oncology
Huisi Liu, Sanduo Zheng, Xinfeng Hou, Ximing Liu, Kaixin Du, Xueyuan Lv, Yulu Li, Fang Yang, Wenhui Li, Jianhua Sui
Article
Medicine, Research & Experimental
Shi-Bing Li, Yan-Yan Liu, Li Yuan, Ming-Fang Ji, Ao Zhang, Hui-Yu Li, Lin-Quan Tang, Shuo-Gui Fang, Hua Zhang, Shan Xing, Man-Zhi Li, Qian Zhong, Shao-Jun Lin, Wan-Li Liu, Peng Huang, Yi-Xin Zeng, Yu-Ming Zheng, Zhi-Qiang Ling, Jian-Hua Sui, Mu-Sheng Zeng
EMBO MOLECULAR MEDICINE
(2020)
Article
Immunology
Fang Yang, Linlin Zhao, Zhizhong Wei, Yajing Yang, Juan Liu, Yulu Li, Xinxin Tian, Ximing Liu, Xueyuan Lu, Jianhua Sui
JOURNAL OF IMMUNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Kaixin Du, Yulu Li, Juan Liu, Wei Chen, Zhizhong Wei, Yong Luo, Huisi Liu, Yonghe Qi, Fengchao Wang, Jianhua Sui
Summary: This study developed a novel bispecific antibody (BsAb) that targets both GPC3 and CD47, demonstrating strong antitumor activity against tumor cells expressing both antigens, with extended serum half-life and enhanced Fc-mediated effector functions in vitro. This approach outperformed monotherapies and a combination therapy in a xenograft HCC model, providing new insights for future antibody design in innovative immune therapies.
Article
Ecology
Huan Yan, Hengwu Jiao, Qianyun Liu, Zhen Zhang, Qing Xiong, Bing-Jun Wang, Xin Wang, Ming Guo, Lin-Fa Wang, Ke Lan, Yu Chen, Huabin Zhao
Summary: The study shows significant variability in susceptibility to SARS-CoV and SARS-CoV-2 infection among bat species. Many bat species may not be potential hosts for these viruses, and there is no correlation between proximity to humans and likelihood of being natural hosts.
NATURE ECOLOGY & EVOLUTION
(2021)
Article
Multidisciplinary Sciences
Zijian Xu, Daoming Chen, Yucheng Hu, Kaiju Jiang, Huanwei Huang, Yingxue Du, Wenbo Wu, Jiawen Wang, Jianhua Sui, Wenhui Wang, Long Zhang, Shuli Li, Chunying Li, Yong Yang, Jianmin Chang, Ting Chen
Summary: The skin serves as a physical barrier and immunological interface that protects the body from external environment. Aberrant activation of immune cells can lead to autoimmune skin diseases like vitiligo, characterized by symmetric lesions. Understanding the role of dermal fibroblast subsets in orchestrating immune activities at an organ level is crucial for autoimmune disease treatment.
Article
Multidisciplinary Sciences
Jianxiu Zhang, Ying-Ying Wang, Zhao-Qian Pan, Yulu Li, Jianhua Sui, Li-Lin Du, Keqiong Ye
Summary: This study reveals the role of Nbr1 in cargo selectivity in autophagy and its binding mechanism with Ams1. The FW domain of Nbr1 recognizes the N-terminal di-glycine and tetrameric assembly of Ams1, while fission yeast Nbr1 uses the ZZ domain to bind Ams1. These findings enrich our understanding of Nbr1-mediated cargo recognition.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
Ximing Liu, Xinxin Tian, Xinyan Hao, Huixiang Zhang, Kailun Wang, Zhizhong Wei, Xin Wei, Yulu Li, Jianhua Sui
Summary: The study successfully developed pH-dependent anti-EGFR antibodies with cross-species reactivity, demonstrating their tumor-selective binding to EGFR under acidic conditions and potential to overcome limitations of antibody binding to normal tissues.
MOLECULAR THERAPY-ONCOLYTICS
(2022)
Review
Virology
Huan Yan, Chunli Wang
Summary: About ten years ago, Wenhui Li's research group in China discovered the sodium taurocholate co-transporting polypeptide (NTCP) as a functional receptor for hepatitis B virus (HBV) and hepatitis delta virus (HDV). This identification led to the establishment of efficient infection models and facilitated in-depth study of HBV entry mechanism and therapeutic development. Follow-up studies, including NTCP structure resolution, further clarified the complex HBV entry process. In this review, the author shares the journey of receptor hunting, highlighting the photo-cross-linking study and successful receptor identification factors.