Article
Virology
Styliani Pappa, Katerina Haidopoulou, Charalampos Zarras, Eleni Theodorakou, Eleni Papadimitriou, Elias Iosifidis, Ioanna Gkeka, Konstantina Stoikou, Eleni Vagdatli, Lemonia Skoura, Anna Papa
Summary: An unexpected surge of RSV infections among children was observed in Greece, with RSV-A being the predominant subtype. This may be attributed to the lack of immunity among children and mothers due to the COVID-19 pandemic. Awareness and molecular epidemiology are crucial for diagnosing and monitoring RSV infections.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Virology
Pramila Lamichhane, Megolhubino Terhuja, Timothy A. Snider, Antonius G. P. Oomens
Summary: The study utilized a novel vaccine to enhance the induction of anti-G antibodies against RSV, resulting in improved protection in mice experiments.
Article
Immunology
Teresia W. Maina, Elizabeth A. Grego, Scott Broderick, Randy E. Sacco, Balaji Narasimhan, Jodi L. McGill
Summary: Researchers determined the effectiveness of a nanovaccine based on polyanhydride, encapsulating the BRSV post-fusion F and G glycoproteins and CpG, in the neonatal calf model. The nanovaccine, delivered via heterologous or homologous immunization, demonstrated clinical and virological protection in the calves, comparable to a commercial modified-live vaccine.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Virology
Vera Krivitskaya, Kseniya Komissarova, Maria Pisareva, Maria Sverlova, Artem Fadeev, Ekaterina Petrova, Veronika Timonina, Anna Sominina, Daria Danilenko
Summary: The study analyzed sequence variability of RSV G gene and G protein from St. Petersburg isolates, finding the majority to be RSV-A and belonging to genotype ON1/GA2, with most isolates falling into the ON1(1.1) and ON1(1.2) sub-clusters, while the ON1(1.3) sub-cluster constituted a minor group. Single-amino acid substitutions were identified in the G proteins of St. Petersburg isolates, mainly in immunodominant B- and T-cell antigenic determinants. These substitutions may impact the antigenic characteristics of RSV and affect the host antiviral immune response.
Article
Virology
Chun-Yi Lee, Yu-Ping Fang, Li-Chung Wang, Teh-Ying Chou, Hsin-Fu Liu
Summary: In this study, the molecular evolution and phylodynamics of respiratory syncytial virus (RSV) in central Taiwan were investigated. The study also analyzed the genetic variability of the commonly used RSV genotypes ON1 and BA. The results revealed that the NA1, ON1, and BA9 genotypes were responsible for the RSV epidemics in central Taiwan.
Article
Virology
Ya-jun Guo, Bing-han Wang, Lin Li, Ya-ling Li, Xiao-li Chu, Wei Li
Summary: This study investigated the epidemiological and genetic characteristics of RSV circulating in Hangzhou after the peak of COVID-19. A total of 267 (21.79%) RSV-positive samples were identified out of 1225 collected nasopharyngeal swabs from outpatients with ARTIs. There was no gender bias, but a clear age preference for infection, with children aged 3-6 years being more susceptible. Phylogenetic analysis revealed that the dominant RSV strains belonged to the ON1 genotype for RSV-A and the BA9 genotype for RSV-B. Ten fixed mutations were also identified, highlighting their importance regarding biological properties.
JOURNAL OF CLINICAL VIROLOGY
(2023)
Article
Infectious Diseases
Chun Yi Lee, Tsung Hua Wu, Yu Ping Fang, Jih Chin Chang, Hung Chun Wang, Shou Ju Lin, Chen Hao Mai, Yu Chuan Chang, Teh Ying Chou
Summary: The delayed 2020-2021 RSV outbreak in Taiwan was caused by two novel RSV ON1.1 variants. The change in RSV epidemiology during this season led to shorter hospital stays, increased oxygen supplements, and increased systemic steroid therapy among patients compared to previous seasons. Further exploration is needed to understand how this change will impact future RSV outbreaks.
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2022)
Review
Virology
Larry J. Anderson, Samadhan J. Jadhao, Clinton R. Paden, Suxiang Tong
Summary: Respiratory syncytial virus (RSV) causes serious respiratory infections in young children and the elderly. The G protein, alongside the F protein, is a key target for vaccine and antiviral drug development due to its role in cellular infection and modulation of immune responses.
Article
Immunology
Xin Cheng, Gan Zhao, Aihua Dong, Zhonghuai He, Jiarong Wang, Brian Jiang, Bo Wang, Miaomiao Wang, Xuefen Huai, Shijie Zhang, Shuangshuang Feng, Hong Qin, Bin Wang
Summary: This study conducted a first-in-human trial to evaluate the safety and immunogenicity response of the BARS13 vaccine in healthy adults. The vaccine demonstrated good safety and tolerability, and there were no significant differences in adverse reaction severity or frequency between different dose groups. The immune response in repeat-dose recipients showed potential for further study and guiding the dose selection for future studies.
Article
Multidisciplinary Sciences
Arang Rhie, Shane A. McCarthy, Olivier Fedrigo, Joana Damas, Giulio Formenti, Sergey Koren, Marcela Uliano-Silva, William Chow, Arkarachai Fungtammasan, Juwan Kim, Chul Lee, Byung June Ko, Mark Chaisson, Gregory L. Gedman, Lindsey J. Cantin, Francoise Thibaud-Nissen, Leanne Haggerty, Iliana Bista, Michelle Smith, Bettina Haase, Jacquelyn Mountcastle, Sylke Winkler, Sadye Paez, Jason Howard, Sonja C. Vernes, Tanya M. Lama, Frank Grutzner, Wesley C. Warren, Christopher N. Balakrishnan, Dave Burt, Julia M. George, Matthew T. Biegler, David Iorns, Andrew Digby, Daryl Eason, Bruce Robertson, Taylor Edwards, Mark Wilkinson, George Turner, Axel Meyer, Andreas F. Kautt, Paolo Franchini, H. William Detrich, Hannes Svardal, Maximilian Wagner, Gavin J. P. Naylor, Martin Pippel, Milan Malinsky, Mark Mooney, Maria Simbirsky, Brett T. Hannigan, Trevor Pesout, Marlys Houck, Ann Misuraca, Sarah B. Kingan, Richard Hall, Zev Kronenberg, Ivan Sovi, Christopher Dunn, Zemin Ning, Alex Hastie, Joyce Lee, Siddarth Selvaraj, Richard E. Green, Nicholas H. Putnam, Ivo Gut, Jay Ghurye, Erik Garrison, Ying Sims, Joanna Collins, Sarah Pelan, James Torrance, Alan Tracey, Jonathan Wood, Robel E. Dagnew, Dengfeng Guan, Sarah E. London, David F. Clayton, Claudio Mello, Samantha R. Friedrich, Peter Lovell, Ekaterina Osipova, Farooq O. Al-Ajli, Simona Secomandi, Heebal Kim, Constantina Theofanopoulou, Michael Hiller, Yang Zhou, Robert S. Harris, Kateryna D. Makova, Paul Medvedev, Jinna Hoffman, Patrick Masterson, Karen Clark, Fergal Martin, Kevin Howe, Paul Flicek, Brian P. Walenz, Woori Kwak, Hiram Clawson, Mark Diekhans, Luis Nassar, Benedict Paten, Robert H. S. Kraus, Andrew J. Crawford, M. Thomas P. Gilbert, Guojie Zhang, Byrappa Venkatesh, Robert W. Murphy, Klaus-Peter Koepfli, Beth Shapiro, Warren E. Johnson, Federica Di Palma, Tomas Marques-Bonet, Emma C. Teeling, Tandy Warnow, Jennifer Marshall Graves, Oliver A. Ryder, David Haussler, Stephen J. O'Brien, Jonas Korlach, Harris A. Lewin, Kerstin Howe, Eugene W. Myers, Richard Durbin, Adam M. Phillippy, Erich D. Jarvis
Summary: The Vertebrate Genome Project and the international Genome 10K consortium have collaborated to generate high-quality genome assemblies for 16 species representing six major vertebrate lineages, leading to new biological discoveries. Long-read sequencing technologies are essential for maximizing genome quality, and addressing complex repeats and haplotype heterozygosity are crucial for reducing assembly errors and improving completeness of reference genomes. The lessons learned from this project have paved the way for the Vertebrate Genomes Project (VGP), an international effort to generate high-quality, complete reference genomes for all known vertebrate species.
Article
Virology
Ana M. Nunez Castrejon, Sara M. O'Rourke, Lawrence M. Kauvar, Rebecca M. DuBois
Summary: Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract disease in children, the elderly, and immunocompromised individuals. Currently, there are no FDA-approved RSV vaccines. Previous studies have identified engineered RSV G proteins with increased immunogenicity and safety. In this study, we confirmed that one mutant RSV G protein retains high-affinity binding to protective antibodies and has the same three-dimensional structure as the wild-type RSV G protein. These findings support the further development of this engineered RSV G protein as a vaccine antigen.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Ran Jia, Lijuan Lu, Liyun Su, Ziyan Lin, Da Gao, Haiyan Lv, Menghua Xu, Pengcheng Liu, Lingfeng Cao, Jin Xu
Summary: This paper analyzed RSV-positive patients in the Children's Hospital of Fudan University from October 2019 to October 2021 and compared the clinical features before and during COVID-19. The study found an atypical upsurge of RSV infection in the late summer of 2021 after a major suppression in 2020. Males and infants aged under 5 months were the main populations infected with RSV. Less severe clinical outcomes were observed in patients during COVID-19 than before the pandemic.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yi Yang, Zhikun Wu, Zengxiang Wu, Tinyao Li, Zhuo Shen, Xuan Zhou, Xinyi Wu, Guojing Li, Yan Zhang
Summary: In this study, an improved reference genome sequence of an elite asparagus bean variety was achieved through the integration of nanopore ultra-long reads, PacBio high-fidelity reads, and Hi-C technology. The improved assembly demonstrated significant enhancements, including a higher N50 length, a high anchor ratio, and the presence of only one gap. The utilization of the reference genome allowed for the identification of a gene involved in color determination, which has important implications for understanding the breeding process.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Infectious Diseases
Guangmin Chen, Meifang Lan, Sixian Lin, Yanhua Zhang, Dongjuan Zhang, Yuwei Weng, Kuicheng Zheng
Summary: This study explored the genomic characteristics of respiratory syncytial virus (RSV) in Fujian Province, China, and found that the ON1 and BA11 genotypes were the most prevalent in the region. The obtained sequences showed high similarity with the reference strains, and certain amino acid mutations were found in the antigen site and epitope of the fusion (F) protein.
INFECTION GENETICS AND EVOLUTION
(2022)
Article
Virology
Weizhong Chang, Xiaoli Jiao, Hongyan Sui, Suranjana Goswami, Brad T. Sherman, Caroline Fromont, Juan Manuel Caravaca, Bao Tran, Tomozumi Imamichi
Summary: This study assembled and annotated the complete genome of HSV-2 strain G using PacBio long sequencing reads. It analyzed the 'alpha' sequence and identified packaging signals homologous to HSV-1, as well as determined the termini and cleavage site of the linear genome. The study also visualized four HSV-2 genome isomers using Oxford Nanopore Technology sequencing reads. Additionally, the coding sequences of strain G were compared with other HSV-2 strains and a phylogenetic analysis was conducted.
