Article
Biotechnology & Applied Microbiology
Xubing Long, Ziwei Yang, Yang Li, Qinqin Sun, Xiaojuan Li, Ersheng Kuang
Summary: The study reveals that BRLF1 regulates cellular and viral gene expression and transcription during EBV primary infection, impacting pathways such as DNA replication and metabolism. BRLF1 binds to transcription factors and spliceosome components, influencing RNA polymerase II-dependent transcription and pre-mRNA splicing.
Article
Microbiology
Quincy Rosemarie, Elijah Kirschstein, Bill Sugden
Summary: We have discovered how Epstein-Barr virus (EBV) induces the reorganization of cellular chromatin, with viral DNA replication occurring in the chromatin-free regions. Five families of DNA viruses induce similar reorganization, and they infect a variety of hosts. The viral genes and processes required for EBV's chromatin reorganization have been identified, and a two-step model for the process has been proposed. The importance of chromatin reorganization in viral life cycles is emphasized. Overall, a 9 out of 10 score is given for the importance of this research.
Article
Microbiology
Rachel Hood Edwards, Nancy Raab-Traub
Summary: This study evaluated the impact of EBV and BART lncRNAs on cellular expression, showing that they induce changes in cellular miRs and lncRNAs. In vivo expression changes reflect increased cellular miRs in all tumors, while the BART lncRNA may inhibit the function of induced cellular miRs. The study provides insights into the total effects of EBV and BART lncRNA on cellular RNA expression.
Article
Biochemistry & Molecular Biology
Isak Holmqvist, Alan Backerholm, Yarong Tian, Guojiang Xie, Kaisa Thorell, Ka-Wei Tang
Summary: FLAME is a Python-based bioinformatics pipeline for comprehensive analysis of alternative splicing of gene-specific or transcriptome-wide long-read sequencing data. By combining nanopore sequencing reads with short-read sequencing data, FLAME can effectively resolve the spliceome of RPMS1. The study identified 32 novel exons, including two intron retentions and a cassette exon, within the RPMS1 gene.
Article
Multidisciplinary Sciences
Yoshimi Enose-Akahata, Limin Wang, Fahad Almsned, Kory R. Johnson, Yair Mina, Joan Ohayon, Xin Wei Wang, Steven Jacobson
Summary: Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS), and the cause of MS remains unknown. This study investigated the antibody responses against various viruses in the cerebrospinal fluid (CSF) and serum of MS patients, and found significant differences compared to healthy volunteers, as well as a pattern of antibody responses against multiple viruses, including Epstein-Barr virus. These findings indicate that virus-specific antibody signatures might reflect the disease-associated inflammatory milieu in the CSF of patients with neuroinflammatory diseases.
Article
Pathology
Sudipta Samanta, Muthukaruppan Swaminathan, Jianing Hu, Khai Tuck Lee, Ajitha Sundaresan, Chuan Keng Goh, Chor Hiang Siow, Kwok Seng Loh, Soh Ha Chan, Joshua K. Tay, Ian Cheong
Summary: The use of immunofluorescence assay for detecting serum Epstein-Barr virus antibodies is the gold standard screening test for nasopharyngeal cancer in high-risk populations. This study demonstrates that integrating deep learning with automated fuzzy inference can improve the scalability and accuracy of NPC detection.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Virology
Mingqian Xu, Liang Zhang, Jinfu Feng, Shuaibing Yang, Yixuan Wang, Yuyi Wang, Meiyang Chen, Li Zhou, Junjie Zhang, Qingsong Qin
Summary: EBV-associated gastric cancer is a subtype of gastric cancer characterized by the presence of EBV genome and limited viral gene expression in malignant epithelial cells. Two EBV-positive gastric cancer cell lines have been established to study the role of EBV in different contexts of gastric carcinogenesis. These cell lines can serve as models to investigate the role of EBV in gastric cancer and identify new therapeutics against EBVaGC.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Hematology
Hannes Vietzen, Philippe L. Furlano, Jan J. Cornelissen, Georg A. Boehmig, Peter Jaksch, Elisabeth Puchhammer-Stoeckl
Summary: This study found that nonclassic human leukocyte antigen E (HLA-E)-restricted immune responses have a significant impact on the development of Epstein-Barr virus (EBV) diseases in the individual host. The highly expressed HLA-E*0103/0103 genotype is protective against infectious mononucleosis (IM) by inducing potent EBV BZLF1-specific HLA-E-restricted CD8(+) T-cell responses. Variations in the inhibitory NKG2A/LMP-1/HLA-E axis are associated with the risk of symptomatic EBV reactivations in both immunocompetent individuals and immunocompromised transplant recipients.
Article
Multidisciplinary Sciences
Munetoshi Hinata, Tetsuo Ushiku
Summary: A histology-based deep learning model was constructed to efficiently screen EBV and MSI/dMMR subtypes in gastric cancers, showing high accuracy in detecting the subgroup and highlighting intraepithelial lymphocytosis as a discriminative characteristic shared by EBV and MSI/dMMR tumors. This model offers a potential economical and less time-consuming alternative for stratifying patients who respond to ICIs.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Francesco Baccianti, Charlene Masson, Susanne Delecluse, Zhe Li, Remy Poirey, Henri-Jacques Delecluse
Summary: EBV efficiently infects and transforms B lymphocytes by activating viral gene expression and manipulating cellular pathways to limit the immune response, according to this study. The viral particle components sequentially activate proinflammatory B lymphocyte signaling pathways, leading to viral gene expression and cytokine release. However, infection also triggers the expression of ZFP36L1, a stress protein that limits the cytokine response. This study highlights the complex interactions between EBV and host cells during infection and transformation.
Article
Biochemical Research Methods
Hansaim Lim, Chung-Jung Tsai, Ozlem Keskin, Ruth Nussinov, Attila Gursoy
Summary: HMI-PRED 2.0 is a publicly available web service that predicts host-microbe protein-protein interaction using interface mimicry. It aims to be user-friendly without requiring extensive computational experience.
Article
Medicine, General & Internal
Yin Xu, Ayako Hiyoshi, Kelsi A. Smith, Fredrik Piehl, Tomas Olsson, Katja Fall, Scott Montgomery
Summary: The study suggests that having infectious mononucleosis in childhood and especially adolescence is a risk factor associated with a diagnosis of multiple sclerosis, independent of shared familial factors.
Article
Multidisciplinary Sciences
Linlin Zhang, Mengjia Liu, Meng Zhang, Junhong Ai, Jiao Tian, Ran Wang, Zhengde Xie
Summary: Infectious mononucleosis (IM) is an acute syndrome associated with primary Epstein-Barr virus (EBV) infection. The exact pathogenic mechanism of IM remains unclear and there is currently no effective treatment. B cells and NK cells play important roles in IM, while the balance between CD4+ T cells and CD8+ T cells is crucial for lifelong control of the disease. Transcriptome sequencing of different cell populations involved in IM can help understand their functions and pathogenic mechanisms.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Immunology
Mariann Gyoengyoesi, Dominika Lukovic, Julia Mester-Tonczar, Katrin Zlabinger, Patrick Einzinger, Andreas Spannbauer, Victor Schweiger, Katharina Schefberger, Eslam Samaha, Jutta Bergler-Klein, Martin Riesenhuber, Christian Nitsche, Christian Hengstenberg, Patrick Mucher, Helmuth Haslacher, Monika Breuer, Robert Strassl, Elisabeth Puchhammer-Stoeckl, Christian Loewe, Dietrich Beitzke, Ena Hasimbegovic, Thomas A. Zelniker
Summary: This study evaluated the reactivation of parvovirus-B19 and Herpesviridae family members in patients with long-COVID syndrome and found that vaccination with monovalent COVID-19 vaccines was associated with reduced fatigue and multiorgan symptoms. It also led to lower levels of DNA virus-related IgM, plasma IgG subfractions, cytomegalovirus IgG and IgM, and EBV IgM titers.
Article
Immunology
Guang-Qiang Meng, Jing-Shi Wang, Yi-Ni Wang, Na Wei, Zhao Wang
Summary: These findings suggest that rituximab-containing regimens are effective in treating EBV-HLH patients with predominately B cell infection, especially for eliminating EBV. However, the treatment may not be as effective for patients with T and NK cell predominated EBV infection.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Editorial Material
Immunology
Alexander J. Sundermann, Cornelius J. Clancy, A. William Pasculle, Guojun Liu, Richard B. Cumbie, Eileen Driscoll, Ashley Ayres, Lisa Donahue, Steven A. Pergam, Lilian Abbo, David R. Andes, Pranatharthi Chandrasekar, Alison L. Galdys, Kimberly E. Hanson, Kieren A. Marr, Jeanmarie Mayer, Seema Mehta, Michele I. Morris, John Perfect, Sanjay G. Revankar, Becky Smith, Sankar Swaminathan, George R. Thompson, Merin Varghese, Jose Vazquez, Estella Whimbey, John R. Wingard, M. Hong Nguyen
CLINICAL INFECTIOUS DISEASES
(2019)
Article
Virology
Wenmin Fu, Dinesh Verma, Ashlee Burton, Sankar Swaminathan
JOURNAL OF VIROLOGY
(2019)
Article
Microbiology
Dajiang Li, Sankar Swaminathan
Meeting Abstract
Endocrinology & Metabolism
Rahul Agrawal, Michael G. Montgomery, Dinesh Verma, Griffin T. Durupt, Adriana Vieira de Abreu, Sankar Swaminathan, Simon Fisher
Article
Virology
Dajiang Li, Tim Mosbruger, Dinesh Verma, Sankar Swaminathan
JOURNAL OF VIROLOGY
(2020)
Editorial Material
Immunology
Priya Swaminathan, Sankar Swaminathan
OPEN FORUM INFECTIOUS DISEASES
(2020)
Article
Multidisciplinary Sciences
Dinesh Verma, Trenton Mel Church, Sankar Swaminathan
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Immunology
Rebekah M. Martin, Kristina Burke, Dinesh Verma, Heng Xie, Janine Langer, Robert Schlaberg, Sankar Swaminathan, Kimberly E. Hanson
OPEN FORUM INFECTIOUS DISEASES
(2020)
Article
Sport Sciences
Kimberly G. Harmon, Paul S. Pottinger, Aaron L. Baggish, Jonathan A. Drezner, Andrew M. Luks, Alexis A. Thompson, Sankar Swaminathan
SPORTS HEALTH-A MULTIDISCIPLINARY APPROACH
(2020)
Article
Virology
Dinesh Verma, Trenton Mel Church, Sankar Swaminathan
Summary: Understanding the factors that affect the infectivity of SARS-CoV-2 is crucial in combating COVID-19. This study showed that Epstein-Barr virus (EBV) infection induced ACE2 expression in epithelial cells, increasing susceptibility to SARS-CoV-2. The EBV transcriptional activator Zta directly activated the ACE2 promoter, and inhibiting EBV replication may reduce susceptibility to SARS-CoV-2.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Elena G. Gibson, Melissa Pender, Michael Angerbauer, Craig Cook, Barbara Jones, Adam M. Spivak, Emily S. Spivak, Sankar Swaminathan
Summary: This case highlights the potential risk of persistent SARS-CoV-2 infection in patients treated with anti-CD-20 monoclonal antibodies, and the potential effectiveness of convalescent plasma and antiviral therapy in resolving symptoms. It suggests a significant role for humoral immunity in disease resolution.
OPEN FORUM INFECTIOUS DISEASES
(2021)
Article
Immunology
Melissa Pender, Ninad Mehta, Blake D. Hamilton, Sankar Swaminathan
Summary: We describe the case of a poorly controlled type 2 diabetes mellitus patient who presented with left-sided abdominal pain and was found to have a left adrenal abscess caused by Nocardia beijingensis. We review the published literature on this uncommonly identified Nocardia species among immunocompetent patients and summarize the diagnosis and management of N beijingensis isolated from an adrenal abscess.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Sport Sciences
Kimberly Harmon, Anabelle M. de St Maurice, Adam C. Brady, Sankar Swaminathan, Doug F. Aukerman, Miguel A. Rueda, Kim Terrell, Randall P. Cohen, Seth C. Gamradt, Sunday D. Henry, Lindsay M. Huston, David R. McAllister, Kenneth M. McCarty, Anthony N. Pass, Stephen R. Paul, David J. Petron, Stephanie A. Kliethermes
Summary: This study evaluated the diagnostic accuracy of antigen testing compared with RT-PCR in an asymptomatic athlete screening program, finding that antigen testing has high specificity but lower sensitivity than RT-PCR. Daily antigen testing or RT-PCR testing two to three times a week showed similar effectiveness in identifying infections, with benefits and drawbacks to each approach.
BMJ OPEN SPORT & EXERCISE MEDICINE
(2021)
Article
Medicine, Research & Experimental
Rahul Agrawal, Griffin Durupt, Dinesh Verma, Michael Montgomery, Adriana Vieira-de Abreu, Casey Taylor, Sankar Swaminathan, Simon J. Fisher
Meeting Abstract
Infectious Diseases
M. Hong Nguyen, Cornelius Clancy, William Pasculle, Merin Varghese, Becky Smith, John Perfect, Kirk Huslage, L. Abbo, Kieran Marr, Seema Mehta, Jose Vazquez, Stephen Pergam, George R. Thompson, Peter Pappas, John Wingard, Michelle Morris, Allison Galdys, Kimberly Hanson, Jeanmarie Mayer, Sankar Swaminathan, Estella Whimbey, David Andes, Pranathar Chandrasekar, Sanjay Revankar, M. Hong Nguyen
