Article
Virology
Han Cao, Jinyuan Wu, Ning Luan, Yunfei Wang, Kangyang Lin, Cunbao Liu
Summary: The study found that NSP4 as an antigen in recombinant specific vaccines can provide strong protection, especially in rotavirus-induced diarrhea mouse models. However, its effect as an adjuvant in increasing neutralizing antibody production is limited.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Naohiro Kon, Hsin-tzu Wang, Yoshiaki S. Kato, Kyouhei Uemoto, Naohiro Kawamoto, Koji Kawasaki, Ryosuke Enoki, Gen Kurosawa, Tatsuto Nakane, Yasunori Sugiyama, Hideaki Tagashira, Motomu Endo, Hideo Iwasaki, Takahiro Iwamoto, Kazuhiko Kume, Yoshitaka Fukada
Summary: Temperature compensation of circadian rhythms is achieved through NCX-mediated Ca2+ signaling, which plays essential roles in maintaining temperature-compensated oscillations.
Article
Multidisciplinary Sciences
Pere Catala, Nathalie Groen, Vanessa L. S. LaPointe, Mor M. Dickman
Summary: The primary culture of corneal endothelial cells (CECs) has limitations in terms of limited expansion and assessing the quality of the culture. Through single-cell RNA sequencing, this study identified the transcriptomic changes and suggested markers to evaluate the quality of primary CEC cultures. This research provides a better understanding of cellular heterogeneity and lays the foundation for improving culture protocols and therapies.
SCIENTIFIC REPORTS
(2023)
Article
Pediatrics
Sarah-Blythe Ballard, David Requena, Holger Mayta, Gerardo J. Sanchez, Maria G. Oyola-Lozada, Fabiola D. Colquechagua Aliaga, Lilia Cabrera, Macarena D. Vittet Mondonedo, Carmen Taquiri, Capt Drake H. Tilley, Cdr Mark P. Simons, Rina A. Meza, Caryn Bern, Mayuko Saito, Dante A. Figueroa-Quintanilla, Robert H. Gilman
Summary: This study aims to understand the changes in enteropathogen landscape after the implementation of rotavirus vaccine in order to reduce pediatric diarrhea burden. Through a case-control study in Peru, it was found that norovirus, rotavirus, sapovirus, astrovirus, enterotoxigenic Escherichia coli, and Shigella spp. were positively associated with gastroenteritis. Norovirus was identified as the leading cause of pediatric gastroenteritis, while sapovirus was often overlooked. Both viruses significantly contribute to the burden of severe pediatric disease in this setting. Decision-makers should consider acquiring multivalent vaccines to target multiple caliciviruses in similar countries after successful rotavirus vaccine implementation.
Article
Genetics & Heredity
Meer Jacob Rahimi, Nicole Urban, Meret Wegler, Heinrich Sticht, Michael Schaefer, Bernt Popp, Frank Gaunitz, Manuela Morleo, Vincenzo Nigro, Silvia Maitz, Grazia M. S. Mancini, Claudia Ruivenkamp, Eun-Kyung Suk, Tobias Bartolomaeus, Andreas Merkenschlager, Daniel Koboldt, Dennis Bartholomew, Alexander P. A. Stegmann, Margje Sinnema, Irma Duynisveld, Ramona Salvarinova, Simone Race, Bert B. A. de Vries, Aurelien Trimouille, Sophie Naudion, Daphna Marom, Uri Hamiel, Noa Henig, Florence Demurger, Nils Rahner, Enrika Bartels, J. Austin Hamm, Abbey M. Putnam, Richard Person, Rami Abou Jamra, Henry Oppermann
Summary: Variants in the ATP2B1 gene lead to neurodevelopmental disorders characterized by global development delay, autism, seizures, and limb abnormalities. These variants result in a significant decrease in the export capacity of calcium ions and incorrect cellular localization of ATP2B1.
AMERICAN JOURNAL OF HUMAN GENETICS
(2022)
Article
Hematology
Nan Wang, Jing Yin, Na You, Shangda Yang, Dan Guo, Yangyang Zhao, Yongxin Ru, Xiaoyan Liu, Hui Cheng, Qian Ren, Tao Cheng, Xiaotong Ma
Summary: The transcription factor TWIST1 is identified as a critical regulator of HSC maintenance by modulating mitochondrial function. Deletion of Twist1 leads to decreased HSC frequency, impaired self-renewal capacity, skewed myeloid differentiation, and compromised stress tolerance. The mechanism involves activation of voltage-gated calcium channel Cacna1b, resulting in increased mitochondrial calcium levels, metabolic activity, and reactive oxygen species production, which can be rescued by calcium channel blocker under stress conditions.
Article
Immunology
J. Nakawesi, S. This, J. Hutter, M. Boucard-Jourdin, V. Barateau, K. Getachew Muleta, L. J. Gooday, K. Fog Thomsen, A. Garcias Lopez, I. Ulmert, D. Poncet, B. Malissen, H. Greenberg, O. Thaunat, T. Defrance, H. Paidassi, K. Lahl
Summary: The study reveals that BATF3-dependent cDC1 cells are critical for the induction of rotavirus-specific IgA responses in the mesenteric lymph nodes, through the selective expression of the TGF beta-activating integrin alpha v beta 8. In contrast, cDC2 cells are dispensable for this process, suggesting that the capacity of DC subsets to induce intestinal IgA responses is context-dependent.
MUCOSAL IMMUNOLOGY
(2021)
Review
Neurosciences
Kenneth T. MacLeod
Summary: The heart shows great phenotypic plasticity in adapting to disease and loss of tissue, but the long-term consequences can lead to heart failure and poor prognosis. Changes in ion channels and transporters compromise the efficiency of Ca2+ release from the sarcoplasmic reticulum and increase the likelihood of arrhythmias. These adaptations come at the expense of the heart's overall function and can activate hypertrophic and apoptotic pathways.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Pharmacology & Pharmacy
Christine Hum, Julia Loiselle, Nadine Ahmed, Tyler A. Shaw, Caroline Toudic, John Paul Pezacki
Summary: Investigations into the interaction between miRNAs and SARS-CoV-2 could potentially lead to the development of miRNA-based antiviral therapies as an alternative strategy to combat COVID-19.
Article
Multidisciplinary Sciences
Arthur Neuberger, Kirill D. Nadezhdin, Alexander I. Sobolevsky
Summary: TRPV6 is a calcium-selective ion channel with potential as a drug target. The study presents cryo-EM structures of TRPV6 with inhibitors ruthenium red and econazole, revealing their mechanisms of inhibition. Both inhibitors induce conformational changes in TRPV6, guiding the design of new inhibitors.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Wang-Xia Wang, Paresh Prajapati, Hemendra J. Vekaria, Malinda Spry, Amber L. Cloud, Patrick G. Sullivan, Joe E. Springer
Summary: The study found that mitochondria-enriched miRNAs showed persistent alterations in injured hippocampal cells in rats after severe controlled cortical impact injury, while miRNAs not enriched in mitochondria did not exhibit significant changes in mitochondria. Nanoparticle delivery of miR-146a showed potential in modulating pro-inflammatory effectors in the injured brain.
NEURAL REGENERATION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Edouard Charlebois, Kostas Pantopoulos
Summary: Hepatocellular iron overload suppresses hepcidin production by inhibiting the SMAD and STAT3 signaling pathways downstream of their respective ligands.
Article
Multidisciplinary Sciences
Laura Codarri Deak, Valeria Nicolini, Masao Hashimoto, Maria Karagianni, Petra C. Schwalie, Laura Lauener, Eleni Maria Varypataki, Marine Richard, Esther Bommer, Johannes Sam, Stefanie Joller, Mario Perro, Floriana Cremasco, Leo Kunz, Emilio Yanguez, Tamara Husser, Ramona Schlenker, Marisa Mariani, Vinko Tosevski, Sylvia Herter, Marina Bacac, Inja Waldhauer, Sara Colombetti, Xavier Gueripel, Stephan Wullschleger, Melanie Tichet, Douglas Hanahan, Haydn T. Kissick, Stephane Leclair, Anne Freimoser-Grundschober, Stefan Seeber, Volker Teichgraber, Rafi Ahmed, Christian Klein, Pablo Umana
Summary: The study shows that PD1-IL2v can induce the differentiation of stem-like CD8(+) T cells into better effector cells by binding to PD-1, without the need for CD25 binding. PD1-IL2v has superior efficacy compared to PD-1 or PD-L1 blocking antibodies, as it avoids the accumulation of terminally differentiated and exhausted T cells.
Article
Biochemistry & Molecular Biology
Lu Tang, Qiang Lin, Yanhong Zhang
Summary: This study revealed that TBT disrupts hepatic lipid homeostasis in seahorses by promoting lipid synthesis and inhibiting lipid droplet breakdown.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Immunology
Hong Zheng, Aditya M. Rao, Denis Dermadi, Jiaying Toh, Lara Murphy Jones, Michele Donato, Yiran Liu, Yapeng Su, Cheng L. Dai, Sergey A. Kornilov, Minas Karagiannis, Theodoros Marantos, Yehudit Hasin-Brumshtein, Yudong D. He, Evangelos J. Giamarellos-Bourboulis, James R. Heath, Purvesh Khatri
Summary: Viral infections trigger a distinct host response, with severe infections associated with increased hematopoiesis, myelopoiesis, and myeloid-derived suppressor cells. Protective and detrimental gene modules were identified to define different trajectories of outcomes, providing insights for the development of diagnostics and host-directed therapies to improve global pandemic preparedness.
Article
Biotechnology & Applied Microbiology
Ana C. Alcala, Raiza Hernandez-Bravo, Fernando Medina, David S. Coll, Jose L. Zambrano, Rosa M. del Angel, Juan E. Ludert
JOURNAL OF GENERAL VIROLOGY
(2017)
Article
Virology
Esmeralda Vizzi, Oscar A. Pineros, M. Daniela Oropeza, Laura Naranjo, Jose A. Suarez, Rixio Fernandez, Jose L. Zambrano, Argelia Celis, Ferdinando Liprandi
Article
Virology
Cinthia L. Dionicio, Franshelle Pena, Luis A. Constantino-Jonapa, Carlos Vazquez, Martha Yocupicio-Monroy, Romel Rosales, Jose Luis Zambrano, Marie Christine Ruiz, Rosa M. del Angel, Juan E. Ludert
Article
Virology
Esmeralda Vizzi, Oscar Pineros, German Gabriel Gonzalez, Jose L. Zambrano, Juan E. Ludert, Ferdinando Liprandi
JOURNAL OF MEDICAL VIROLOGY
(2011)
Article
Multidisciplinary Sciences
Jose Luis Zambrano, Orlando Sorondo, Ana Alcala, Esmeralda Vizzi, Yuleima Diaz, Marie Christine Ruiz, Fabian Michelangeli, Ferdinando Liprandi, Juan E. Ludert
Review
Virology
Joseph T. Ortega, Jose L. Zambrano, Beata Jastrzebska, Ferdinando Liprandi, Hector R. Rangel, Flor H. Pujol
Article
Multidisciplinary Sciences
Carmen L. Loureiro, Rossana C. Jaspe, Pierina D. Angelo, Jose L. Zambrano, Lieska Rodriguez, Victor Alarcon, Mariangel Delgado, Marwan Aguilar, Domingo Garzaro, Hector R. Rangel, Flor H. Pujol
Summary: This study aimed to genetically characterize Venezuelan SARS-CoV-2 isolates, focusing on the high diversity and predominance of the D614G mutation in the circulating isolates. The findings showed that isolates from the first outbreak in Margarita Island had a unique deletion in the NSP1 gene, but were not responsible for the larger outbreak that occurred later.
Article
Infectious Diseases
Rossana C. Jaspe, Carmen L. Loureiro, Yoneira Sulbaran, Zoila C. Moros, Pierina D'Angelo, Lieska Rodriguez, Jose Luis Zambrano, Mariana Hidalgo, Esmeralda Vizzi, Victor Alarcon, Marwan Aguilar, Domingo J. Garzaro, Hector R. Rangel, Flor H. Pujol
Summary: The study aimed to monitor the introduction of variants and VOCs in Venezuela, with the detection of the Gamma VOC since January 2021, which became predominant in the country by March 2021. Additionally, other isolates carrying the E484K mutation were also detected.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Multidisciplinary Sciences
Mercedes Viettri, Jose L. Zambrano, Romel Rosales, Gerson I. Caraballo, Ana Lorena Gutierrez-Escolano, Juan E. Ludert
Summary: The study investigated whether infection with dengue and Zika viruses triggers a Golgi stress response in vertebrate and mosquito cells, showing that these viruses induce expansion of the Golgi complex and activation of the TFE3 pathway.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, Research & Experimental
Rossanct C. Jaspe, Jose Luis Zambrano, Mariana Hidalgo, Yoneira Sulbaran, Carmen L. Loureiro, Zoila C. Moros, Domingo J. Garzaro, Ferdinando Liprandi, Hector R. Rangel, Flor H. Pujol
Summary: This study describes a rapid method for identifying cases infected with the Omicron variant and provides more information on the prevalence and rapid detection of this new variant.
INVESTIGACION CLINICA
(2022)
Article
Virology
Rossana C. Jaspe, Carmen L. Loureiro, Yoneira Sulbaran, Zoila C. Moros, Pierina D'Angelo, Mariana Hidalgo, Lieska Rodriguez, Victor Alarcon, Marwan Aguilar, Doneyla Sanchez, Jesus Ramirez, Domingo J. Garzaro, Jose Luis Zambrano, Ferdinando Liprandi, Hector R. Rangel, Flor H. Pujol
Summary: The aim of this study was to monitor the dissemination of VOIs and VOCs in Venezuela, and it found that Delta VOC became the predominant circulating variant, while Omicron VOC was able to displace Delta variant within a month.
Article
Medicine, Research & Experimental
Jose Luis Zambrano, Rossana C. Jaspe, Mariana Hidalgo, Carmen L. Loureiro, Yoneira Sulbaran, Zoila C. Moros, Domingo J. Garzaro, Esmeralda Vizzi, Hector R. Rangel, Ferdinando Liprandi, Flor H. Pujol
Summary: This study describes the characteristic mutations of the Omicron variant in the Spike protein and the behavior of the sub-lineages in different parts of the world. The mutations in the Spike protein may enable the virus to evade vaccine protection, reduce susceptibility to host proteases for fusion process, and may be associated with enhanced replication in nasal epithelial cells and decreased replication in pulmonary tissue, which may explain the apparent reduced severity of Omicron compared to other variants.
INVESTIGACION CLINICA
(2022)
Article
Infectious Diseases
Pierina D'Angelo, Carmen L. L. Loureiro, Rossana C. C. Jaspe, Yoneira F. F. Sulbaran, Lieska Rodriguez, Victor Alarcon, Jose Manuel Garcia, Jose Luis Zambrano, Ferdinando Liprandi, Hector R. Rangel, Flor H. H. Pujol
Summary: The ongoing epidemic of monkeypox virus (MPXV) infection has affected over 50,000 individuals worldwide until the end of August 2022. We present the first case detected in Venezuela, where the patient had traveled from Spain and had contact with friends who tested positive for MPXV. Phylogenetic analysis categorized the isolate within the major circulating clade II of MPXV globally. As of August 2022, no other cases of MPXV have been detected in Venezuela, though undiagnosed cases cannot be ruled out due to the fear of stigmatization.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Virology
Zoila C. Moros, Jose Luis Zambrano, Yoneira Sulbaran, Carmen L. Loureiro, Ernestina Marulanda, Francis Bracho, Pierina D'Angelo, Lieska Rodriguez, Ferdinando Liprandi, Hector R. Rangel, Rossana C. Jaspe, Flor H. Pujol
Summary: The Omicron Variant of Concern (VOC) has caused a surge in cases worldwide, leading to subsequent waves in many countries. Venezuela has monitored the circulation of the Omicron VOC and its major sub-lineages among community cases and international travelers. Sub-lineages generally circulated earlier in international travelers than in community cases, and the diversity of sub-lineages found in international travelers resembled those found in the USA.
Article
Chemistry, Multidisciplinary
M. Diaz, D. S. Coll, A. Sierraalta, J. L. Zambrano Rouvier, F. Salazar
Summary: This study investigates the theoretical descriptors of different drugs to explore their impacts on the in-vitro antiviral activities against SARS-CoV-2. The results show a moderate correlation between the hardness of the drugs and their biological activity, indicating that electrostatic interactions may dominate in the virus hosting sites with low polarizability.
PHYSICAL CHEMISTRY RESEARCH
(2022)