Article
Microbiology
Yifei Liao, Blanca Lupiani, Mohammad AI-Mahmood, Sanjay M. Reddy
Summary: Marek's disease virus (MDV) is a potent oncogenic alphaherpesvirus that causes T-cell lymphomas in chickens. The U(S)3 protein kinase of MDV plays a crucial role in virus replication and pathogenicity. This study identified novel substrates for U(S)3 and characterized its impact on MDV pathogenesis.
Correction
Multidisciplinary Sciences
Rosamaria Pennisi, Maria Musarra-Pizzo, Zhixiang Lei, Grace Guoying Zhou, Maria Teresa Sciortino
Summary: The paper has been amended and the revised version can be accessed through a link at the top of the paper.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Michiel van Gent, Jessica J. Chiang, Santoshi Muppala, Cindy Chiang, Walid Azab, Lisa Kattenhorn, David M. Knipe, Nikolaus Osterrieder, Michaela U. Gack
Summary: Recent studies show that herpes simplex virus 1 (HSV-1) can evade the immune response by phosphorylating the cytosolic pathogen sensor RIG-I, keeping it in a signaling-repressed state.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Bishajit Sarkar, Md. Asad Ullah, Yusha Araf, Sowmen Das, Md. Hasanur Rahman, Abu Tayab Moin
Summary: HSV is a highly infectious virus with two types, HSV-1 and HSV-2, infecting millions worldwide. This study designed three polyvalent subunit vaccines targeting multiple strains of HSV, which showed stability and potential effectiveness through molecular docking and simulation studies. Validation of these vaccines in wet lab-based studies is still needed.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Microbiology
Liyao Deng, Jieyu Wan, Anchun Cheng, Mingshu Wang, Bin Tian, Ying Wu, Qiao Yang, Xumin Ou, Sai Mao, Di Sun, Shaqiu Zhang, Dekang Zhu, Renyong Jia, Shun Chen, Mafeng Liu, Xinxin Zhao, Juan Huang, Qun Gao, Yanling Yu, Ling Zhang, Leichang Pan
Summary: This study revealed that DPV UL47 protein is a phosphorylated substrate of US3 protein, and the phosphorylation regulates the cytoplasmic localization of UL47. Additionally, US3 protein promotes the cytoplasmic localization of UL47 during DPV infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Zhaoqi Wang, Han Yan, Fujie He, Jie Wang, Yang Zhang, Lishan Sun, Cui Hao, Wei Wang
Summary: The natural compound wedelolactone (WDL) exhibits strong inhibitory effects against both HSV-1 and HSV-2 in vitro and in vivo, with mechanisms distinct from acyclovir and involving interference with host genes and signaling pathways. WDL treatment improves survival, attenuates inflammatory symptoms, and reduces viral titres in infected mice.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Chemistry, Multidisciplinary
Pradeep Chopra, Tejabhiram Yadavalli, Francesco Palmieri, Seino A. K. Jongkees, Luca Unione, Deepak Shukla, Geert-Jan Boons
Summary: Inhibition of human heparanase (Hpse) has been found to be a promising therapeutic strategy for preventing the spread of herpes simplex virus (HSV-1). The synthesis of hexa- and octasaccharide inhibitors of Hpse showed potent enzyme inhibition and the presence of 2-O-sulfation on iduronic acid was tolerated. These inhibitors not only blocked viral-induced shedding of cell-surface heparan sulfate (HS) but also inhibited cell migration and proliferation in corneal epithelial cells infected with HSV-1.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Pharmacology & Pharmacy
Yiwei Huang, Yuyun Song, Jichen Li, Changning Lv, Zhe-Sheng Chen, Zhijun Liu
Summary: Human herpes simplex viruses use cell surface receptors such as heparan sulfate, herpesvirus entry mediator, and nectin-1/-2, 3-O-sulfated heparan sulfate to enter host cells. No vaccine or curative treatment is currently available for HSV infections.
DRUG DISCOVERY TODAY
(2022)
Article
Cell Biology
Robert J. J. Jansens, Ruth Verhamme, Aashiq H. Mirza, Anthony Olarerin-George, Cliff Van Waesberghe, Samie R. Jaffrey, Herman W. Favoreel
Summary: Chemical modifications of mRNA, such as N6-methyladenosine (m6A), play a role in post-transcriptional gene regulation. This study found that alphaherpesvirus kinases can trigger phosphorylation of components of the m6A methyltransferase complex, leading to its inhibition and a decrease in m6A levels in virus-infected cells. The viral US3 protein is responsible for this phosphorylation and inhibition, and while inactivation of the m6A methyltransferase complex is not necessary for virus replication, the m6A methylation motif is underrepresented in alphaherpesvirus genomes.
Review
Oncology
Hayle Scanlan, Zachary Coffman, Jeffrey Bettencourt, Timothy Shipley, Debra E. Bramblett
Summary: This review provides an overview of HSV-1 as an oncolytic virus candidate and the genomic organization of T-VEC. The advantages and limitations of T-VEC compared to other HSV-1 oncolytic virus variants currently in clinical trials are discussed. Additionally, future directions for the use of HSV-1 oncolytic viruses as cancer therapy are explored.
FRONTIERS IN ONCOLOGY
(2022)
Article
Pharmacology & Pharmacy
Etienne E. Muller, Dumisile Maseko, Ranmini S. Kularatne
Summary: This study conducted phenotypic and genotypic surveillance on ACV resistance in HSV-2 derived from genital ulcer samples collected in a primary healthcare facility in South Africa. The results revealed no resistance-associated mutations, but identified several known natural polymorphisms and amino acid changes of unknown significance. All cultivable HSV-2 isolates showed susceptibility to ACV.
ANTIVIRAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Leonardo Cassari, Angela Pavan, Giulia Zoia, Monica Chinellato, Elena Zeni, Alessandro Grinzato, Sylvia Rothenberger, Laura Cendron, Monica Dettin, Antonella Pasquato
Summary: The SARS-CoV-2 virus displays unique characteristics and has a high ability to spread among humans. The virus relies on Furin for its invasion and replication, and though there are mutations in the amino acid sequence, the dependence on Furin remains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Aakash Mahant Mahant, Matthew S. Gromisch, Leah Kravets, Clare Burn Aschner, Betsy C. Herold
Summary: A new vaccine strategy was evaluated to protect against Herpes simplex viruses (HSV) by inducing antibody-dependent cell-mediated cytolysis (ADCC) rather than neutralizing antibodies (nAbs). The vaccine provided complete protection against HSV infections in multiple lethal mouse models at different timepoints, while previous vaccines generating nAbs showed limited and transient protection.
Article
Biochemistry & Molecular Biology
Xin Ming, Zongyi Bo, Yurun Miao, Huan Chen, Chenyi Bao, Liumei Sun, Rui Xi, Qiuping Zhong, Pu Zhao, Yong-Sam Jung, Yingjuan Qian
Summary: The PRV infection activates ATM and DNA-PK signaling pathways, while the PRV-encoded kinase UL13 interacts with and phosphorylates H2AX. Deletion of UL13 attenuates PRV neuroinvasiveness and virulence in vivo. UL13-mediated H2AX phosphorylation plays a pivotal role in efficient PRV replication and progeny production.
Article
Virology
Abigail L. Whitford, Corinne A. Clinton, E. B. Lane Kennedy, Sara A. Dochnal, Jon B. Suzich, Anna R. Cliffe
Summary: The cellular protein DLK plays an essential role in the reactivation of herpes simplex virus, and the mechanisms of gene expression during reactivation are distinct from replication.
JOURNAL OF VIROLOGY
(2022)
Editorial Material
Dermatology
Yuri Shimizu, Yorihisa Kotobuki, Noriko Arase, Hisashi Arase, Ichiro Katayama, Manabu Fujimoto
ANNALS OF DERMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tadahiro Suenaga, Yasuko Mori, Tatsuo Suzutani, Hisashi Arase
Summary: This study reveals that Siglec-7, a member of the sialic acid immunoglobulin-like lectin (Siglec) family, plays a crucial role in Varicella-zoster virus (VZV) infection of hematopoietic cells. Siglec-7 binds to VZV envelope glycoprotein B in a sialic acid-dependent manner and mediates VZV infection through membrane fusion. These findings provide valuable insights into the molecular mechanisms underlying VZV infection of hematopoietic cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Yuriko Tachida, Saori Miura, Yui Muto, Hiroyuki Takuwa, Naruhiko Sahara, Akihiro Shindo, Yukio Matsuba, Takashi Saito, Naoyuki Taniguchi, Yasushi Kawaguchi, Hidekazu Tomimoto, Takaomi Saido, Shinobu Kitazume
Summary: The deposition of amyloid P in blood vessels of the brain is commonly observed in Alzheimer's disease patients. However, it is difficult to examine the contribution of cerebral amyloid angiopathy (CAA) to the pathogenesis of Alzheimer's disease in mouse models. Based on biochemical analyses, the researchers hypothesized that endothelial APP expression would be lower in rodents and subsequently generated mice that express human WT APP in endothelial cells. The mice showed increased levels of serum A beta and soluble APP, indicating the crucial role of endothelial APP in blood A beta levels. Crossing these mice with a specific APP knock-in mouse model led to an expanded CAA pathology.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Virology
Fumio Maeda, Akihisa Kato, Kosuke Takeshima, Misato Shibazaki, Ryota Sato, Takuma Shibata, Kensuke Miyake, Hiroko Kozuka-Hata, Masaaki Oyama, Eigo Shimizu, Seiya Imoto, Satoru Miyano, Shungo Adachi, Tohru Natsume, Koh Takeuchi, Yuhei Maruzuru, Naoto Koyanagi, Arii Jun, Kawaguchi Yasushi
Summary: This study developed a screening system to identify cellular proteins involved in the nuclear egress of HSV-1. Through this system, the researchers discovered that the cellular orphan transporter SLC35E1 plays a critical role in HSV-1 de-envelopment.
JOURNAL OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Hui Jin, Kazuki Kishida, Noriko Arase, Sumiko Matsuoka, Wataru Nakai, Masako Kohyama, Tadahiro Suenaga, Ken Yamamoto, Takehiko Sasazuki, Hisashi Arase
Summary: Specific MHC class II alleles are strongly associated with susceptibility to autoimmune diseases. This study found that autoantibodies in patients with Graves' disease preferentially recognize TSHR complexed with MHC class II molecules of Graves' disease risk alleles. It suggests that aberrant TSHR transported by MHC class II molecules is the target of autoantibodies produced in Graves' disease.
Article
Biochemistry & Molecular Biology
Tatsuhiko Ozono, Yasuyoshi Kimura, Tadahiro Suenaga, Goichi Beck, Jyunki Jinno, Cesar Aguirre, Kensuke Ikenaka, Dimitri Krainc, Hideki Mochizuki, Hisashi Arase
Summary: Parkinson's disease (PD) is a neurodegenerative disorder characterized by the accumulation of a-synuclein aggregates. This study identified a new function of HLA class II in transporting a-synuclein outside of cells. Different HLA-DR alleles had varying degrees of translocation of a-synuclein to the cell surface and extracellular vesicles. The findings suggest a potential link between the PD-risk gene HLA-DR and a-synuclein.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Immunology
Ayano Fukui, Yuhei Maruzuru, Kosuke Takeshima, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
Summary: In this study, a system was established to measure the frequency of cell-cell fusion induced by wild-type herpes simplex virus (HSV) strains. It was found that the HSV-1 envelope glycoprotein B and its N-glycosylation at asparagine at position 141 were necessary for efficient cell-cell fusion. This study provides insight into the connection between wild-type HSV-1-induced cell-cell fusion and viral pathogenesis in vivo.
MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Rheumatology
Shanshan Zhang, Hideaki Tsuji, Hui Jin, Koji Kitagori, Shuji Akizuki, Ran Nakashima, Hajime Yoshifuji, Masao Tanaka, Hisashi Arase, Koichiro Ohmura, Akio Morinobu
Summary: This study found that the heavy chain of IgG (IgGH) recognizes the RA-susceptible HLA class II molecule complex through RF. Various types of IgGH segments were synthesized and transfected into human embryonic kidney 293T cells to examine their surface expression and RF recognition. The results showed that the C(H)1 domain of IgGH specifically binds to RA-susceptible HLA-DR molecule and is expressed on the cell surface. RF specifically recognizes the C(H)3 domain of the IgGH/HLA-DR4 complex.
Article
Biochemistry & Molecular Biology
Kazuto Takahashi, Kenji Kanekiyo, Kanoko Sakuda, Yui Muto, Masahiro Iguchi, Nozomu Matsuda, Yuko Hashimoto, Kazuaki Kanai, Haruko Ogawa, Hajime Hirase, Akiyoshi Kakita, Norihisa Bizen, Hirohide Takebayashi, Yasushi Kawaguchi, Miwa Uzuki, Shinobu Kitazume
Summary: Astrocytes, specifically those expressing HNK-1-O-Man(+) PTPRZ, play a crucial role in demyelination-associated conditions such as multiple sclerosis. This unique glycosylation pattern of PTPRZ is responsible for the pattern of reactive astrocytes in damaged brain areas. Understanding the role of HNK-1-O-Man(+) PTPRZ in astrocytes can provide insights into the development and progression of brain diseases.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Cell Biology
Yoshihiko Kuchitsu, Kojiro Mukai, Rei Uematsu, Yuki Takaada, Ayumi Shinojima, Ruri Shindo, Tsumugi Shoji, Shiori Hamano, Emari Ogawa, Ryota Sato, Kensuke Miyake, Akihisa Kato, Yasushi Kawaguchi, Masahiko Nishitani-Isa, Kazushi Izawa, Ryuta Nishikomori, Takahiro Yasumi, Takehiro Suzuki, Naoshi Dohmae, Takefumi Uemura, Glen N. N. Barber, Hiroyuki Arai, Satoshi Waguri, Tomohiko Taguchi
Summary: Stimulator of interferon genes (STING) is crucial for the immune response against DNA pathogens, and its degradation is mediated by endosomal sorting complexes required for transport (ESCRT) proteins. STING-positive vesicles derived from recycling endosomes are encapsulated into lysosomes after STING ubiquitination. This study provides insights into the regulation of STING and prevents hyperactivation of the immune response.
NATURE CELL BIOLOGY
(2023)
Article
Virology
Ahmed H. E. Hassan, Selwan M. M. El-Sayed, Mizuki Yamamoto, Jin Gohda, Takehisa Matsumoto, Mikako Shirouzu, Jun-ichiro Inoue, Yasushi Kawaguchi, Reem M. A. Mansour, Abtin Anvari, Abdelbasset A. A. Farahat
Summary: Coronaviruses and other viruses rely on host proteases for cell entry. Targeting the conserved host-based entry mechanism instead of viral proteins may be advantageous. Compound 14 is identified as a potential starting point for developing viral entry inhibitors.
Article
Immunology
Joseph K. Sampson Duncan, Danyang Xu, Maria Licursi, Michael A. Joyce, Holly A. Saffran, Kaiwen Liu, Jin Gohda, D. Lorne Tyrrell, Yasushi Kawaguchi, Kensuke Hirasawa
Summary: Interferon regulatory factors (IRFs) play important roles in regulating the antiviral response against human coronaviruses. Activation of IRF1, IRF3, and IRF7 is observed during infection with different strains of coronaviruses. Knockdown and overexpression experiments demonstrate that IRF1 and IRF3 have antiviral properties against OC43, while IRF3 and IRF7 are effective in restricting 229E infection. Our study highlights the potential of IRFs as antiviral regulators against human coronavirus infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Seisuke Kusano, Sho Ueda, Daisuke Oryoji, Aya Toyoumi, Akiko Hashimoto-Tane, Hiroyuki Kishi, Hiroshi Hamana, Atsushi Muraguchi, Hui Jin, Hisashi Arase, Hiroko Miyadera, Reiko Kishikawa, Yasunobu Yoshikai, Hisakata Yamada, Ken Yamamoto, Yasuharu Nishimura, Takashi Saito, Takehiko Sasazuki, Shigeyuki Yokoyama
Summary: Cry j 1 is a major allergen in Japanese cedar pollens. The NF region of Cry j 1 peptide plays a role in enhancing T-cell activation. Mutation of Ser and Lys in the NF region to Glu reduces the affinity for HLA-DP5, antigen presentation, and T-cell activation.
INTERNATIONAL IMMUNOLOGY
(2023)
Article
Microbiology
Yujin Inayoshi, Shiori Oguro, Erika Tanahashi, Zhifeng Lin, Yasushi Kawaguchi, Toshiaki Kodama, Chihiro Sasakawa
Summary: A reverse genetics system was established in this study to clone and manipulate the avian infectious bronchitis virus (IBV) genome, providing a useful platform for analyzing the pathogenesis of IBV and developing new vaccines.
CURRENT RESEARCH IN MICROBIAL SCIENCES
(2022)
Article
Virology
Jun Arii, Kosuke Takeshima, Yuhei Maruzuru, Naoto Koyanagi, Yoshitaka Nakayama, Akihisa Kato, Yasuko Mori, Yasushi Kawaguchi
Summary: This study identifies an arginine cluster in the disordered domain of UL34 that interacts with ALIX and recruits ESCRT-III machinery for primary envelopment. The study highlights the importance of the disordered domain of UL34 in herpesvirus infections.
JOURNAL OF VIROLOGY
(2022)