Article
Biochemistry & Molecular Biology
Mitzi Diaz-Hernandez, Rosario Javier-Reyna, Diana Martinez-Valencia, Sarita Montano, Esther Orozco
Summary: By studying the active movement and phagocytosis of the trophozoites of Entamoeba histolytica, researchers have discovered the dynamics of the ESCRT protein interactions during phagocytosis. They identified the proteins forming the E. histolytica ESCRT-II complex and their interactions with other molecules involved in phagocytosis. This study provides valuable insights into the phagocytosis process and the function of related proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jinjin Li, Weiwei Wang, Jinhong Yuan, Jinyu Xu, Lifei He, Xinying Zhang, Haiyan Zhang
Summary: Plants have evolved sophisticated mechanisms to adjust to nutrient deficiency or excess. The COPT2 protein in Arabidopsis thaliana is transcriptionally regulated in response to changing levels of cellular copper, but its activity is subject to degradation in response to excess copper. The protein's stability is affected by proteasome inhibition and its ubiquitination status, highlighting the complexity of plasma membrane transporter degradation mechanisms.
Article
Cell Biology
Shang-Hsin Wu, Mei-Hwei Chang, Ya-Hui Chen, Hui-Lin Wu, Huey-Huey Chua, Chin-Sung Chien, Yen-Hsuan Ni, Hui-Ling Chen, Huey-Ling Chen
Summary: This study identified charged multivesicular body protein 5 (CHMP5) as a molecule that interacts with BSEP, affecting its canalicular targeting and retention, resulting in impaired post-Golgi trafficking and bile acid secretion. These findings suggest that ESCRT-III-mediated BSEP sorting is essential for the trafficking of apical membrane proteins, providing new targets for therapeutic interventions in BSEP-associated cholestasis.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Article
Neurosciences
Jessica A. Lawrence, Patricia Aguilar-Calvo, Daniel Ojeda-Juarez, Helen Khuu, Katrin Soldau, Donald P. Pizzo, Jin Wang, Adela Malik, Timothy F. Shay, Erin E. Sullivan, Brent Aulston, Seung Min Song, Julia A. Callender, Henry Sanchez, Michael D. Geschwind, Subhojit Roy, Robert A. Rissman, JoAnn Trejo, Nobuyuki Tanaka, Chengbiao Wu, Xu Chen, Gentry N. Patrick, Christina J. Sigurdson
Summary: Endolysosomal defects, including the reduction of Hrs and STAM1, exacerbate synaptic derangements and accelerate neurodegeneration in prion-infected brains. Depletion of neuronal Hrs leads to increased surface levels of PrPC, contributing to the rapidly advancing disease through neurotoxic signaling. The findings highlight the importance of proteostatic pathways and synaptic integrity in prion diseases.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Immunology
Ausencio Galindo, Rosario Javier-Reyna, Guillermina Garcia-Rivera, Cecilia Banuelos, Sarita Montano, Jaime Ortega-Lopez, Bibiana Chavez-Munguia, Lizbeth Salazar-Villatoro, Esther Orozco
Summary: The ESCRT complex, consisting of ESCRT-0, ESCRT-I, ESCRT-II, and ESCRT-III complexes, along with accessory proteins, plays a crucial role in vesicular trafficking in eukaryotes. This study focused on the role of the ESCRT-I complex in vesicular trafficking and phagocytosis in Entamoeba histolytica, highlighting the importance of EhVps23 protein in these processes. Results demonstrated the intricate molecular interactions and vesicular structures involved in phagocytosis, emphasizing the pivotal function of EhVps23.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Plant Sciences
Heike Wolff, Marc Jakoby, Lisa Stephan, Eva Koebke, Martin Huelskamp
Summary: The Arabidopsis AAA ATPase SKD1 plays a crucial role in ESCRT-dependent endosomal sorting by disassembling the ESCRTIII complex in an ATP-dependent manner. This study found that SKD1 localizes to messenger ribonucleoprotein complexes under heat stress conditions and interacts with different proteins, including membrane transport proteins and those associated with RNA metabolism. Moreover, upon heat stress, not only RNA associated proteins but also ESCRTIII and membrane trafficking proteins are recruited to messenger ribonucleoprotein granules.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Katherine R. Balka, Rajan Venkatraman, Tahnee L. Saunders, Angus Shoppee, Ee Shan Pang, Zoe Magill, Jihane Homman-Ludiye, Cheng Huang, Rachael M. Lane, Harrison M. York, Peck Tan, Ralf B. Schittenhelm, Senthil Arumugam, Benjamin T. Kile, Meredith O'Keeffe, Dominic De Nardo
Summary: cGAS-STING signalling is initiated by detecting foreign or mislocalised DNA within the cytosol, leading to the production of interferons and inflammatory cytokines. STING is degraded within lysosomes, but the mechanisms controlling its delivery are poorly understood. In this study, the researchers used proteomics and microscopy to identify that the ESCRT pathway detects ubiquitinated STING on vesicles, facilitating its degradation in macrophages. Disruption of the ESCRT pathway enhances STING signalling and cytokine production, revealing a mechanism for effective termination of the pathway.
Article
Multidisciplinary Sciences
Lena Harker-Kirschneck, Anne E. Hafner, Tina Yao, Christian Vanhille-Campos, Xiuyun Jiang, Andre Pulschen, Fredrik Hurtig, Dawid Hryniuk, Sian Culley, Ricardo Henriques, Buzz Baum, Andela Saric
Summary: This study developed a physical model for the division of archaeal cells, which share similarities with eukaryotes. By comparing simulations with live cell imaging experiments, the researchers proposed a previously unidentified division mechanism based on changes in the curvature of elastic cytoskeletal filaments. This study not only reveals this mechanism in archaea, but also suggests its potential application in other biological systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Miriam Baeumers, Katharina Schulz, Thomas Klein
Summary: The ESCRT-III complex plays a role in membrane remodeling in cellular processes, with Shrub and CHMP4 as core members in Drosophila and humans respectively. CHMP4B is identified as the true ortholog of Shrub, while CHMP4A and CHMP4C have diverging activities. LGD proteins' C2 domain has a specific function beyond stability. CHMP4B interacts more efficiently with LGD1 than with LGD2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Tatiana M. Clemente, Rajendra K. Angara, Stacey D. Gilk
Summary: Obligate intracellular pathogens can either reside freely in the host cell cytoplasm or be confined in a membrane-bound vacuole. The pathogens in membrane-bound vacuoles are protected from the innate immune system and antimicrobial drugs, but they face challenges such as obtaining membrane components, maintaining a suitable microenvironment, acquiring essential nutrients from the host cell, and manipulating the host cell through a specialized secretion system.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sascha Pust, Andreas Brech, Catherine Sem Wegner, Harald Stenmark, Kaisa Haglund
Summary: Cellular abscission is the final step of cytokinesis. ALIX and TSG101 proteins are involved in recruiting ESCRT-III to the midbody, and together with CHMP4B, they form spiral-like structures from the midbody to the abscission site. ALIX and CHMP4B are transported in vesicles along microtubules by the kinesin-1 motor protein to the cytokinetic bridge and midbody, contributing to abscission.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Multidisciplinary Sciences
A. King Cada, Mark R. Pavlin, Juan P. Castillo, Alexander B. Tong, Kevin P. Larsen, Xuefeng Ren, Adam L. Yokom, Feng-Ching Tsai, Jamie Shiah, Patricia M. Bassereau, Carlos J. Bustamante, James H. Hurley
Summary: The endosomal sorting complexes required for transport (ESCRT) system is a membrane scission machinery that catalyzes the budding and scission of membranes. In this study, researchers investigated the capability of CHMP1B and IST1, two ESCRT-III subunits, to sever membranes on their own or in concert with VPS4 or spastin. They found that CHMP1B and IST1 can form stable scaffolds on membrane nanotubes but do not lead to scission. However, when an additional extensional force was applied, the CHMP1B-IST1 scaffolded tubes were severed, suggesting a friction-driven scission mechanism. The protein spastin was found to colocalize with CHMP1B-enriched sites but did not disassemble the CHMP1B-IST1 coat from the membrane. VPS4, on the other hand, resolubilized CHMP1B and IST1 without leading to scission. These results demonstrate that CHMP1B-IST1 can sever membranes by a friction-driven mechanism independent of VPS4 and spastin.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Yan Guo, Yuqing Cui, Yangyang Li, Xiaoying Jin, Dandan Wang, Mengxia Lei, Fengzhi Chen, Yali Liu, Jinwen Xu, Guanyu Yao, Guangchun Zeng, Xuesong Chen
Summary: Cytoplasmic YAP1 in breast cancer cells promotes autophagic death and is associated with the survival of breast cancer patients.
CANCER COMMUNICATIONS
(2023)
Review
Immunology
Bethany Vaughn, Yousef Abu Kwaik
Summary: Intravacuolar pathogens possess secretion systems that inject effector proteins into host cells to modulate host processes and remodel their vacuoles. Each pathogen has evolved a unique toolbox of protein effectors to interact with and modulate distinct host cell targets, ultimately generating a unique intravacuolar niche suitable for proliferation.Detailed biochemical and cellular processes affected by these pathogens remain to be fully understood.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biology
Swapneeta S. Date, Peng Xu, Nathaniel L. Hepowit, Nicholas S. Diab, Jordan Best, Boyang Xie, Jiale Du, Eric R. Strieter, Lauren P. Jackson, Jason A. MacGurn, Todd R. Graham
Summary: This study reveals the role of COPI in SNARE trafficking and Golgi SNARE localization through ubiquitin recognition, and demonstrates how ubiquitination enhances the binding between Arf and COPI with Gos1.
Review
Plant Sciences
Ping Zheng, Chunyan Zheng, Marisa S. Otegui, Faqiang Li
Summary: Seed storage proteins (SSPs) play a crucial role in plant science and agriculture, particularly in cereal crops. This review discusses the trafficking routes of SSPs and the key components involved in the endomembrane system in different plant species and tissues. The trafficking pathways in major cereal crops, such as rice and maize, are also compared and contrasted.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Xiaojuan Zhang, Mika Nomoto, Marta Garcia-Leon, Naoki Takahashi, Mariko Kato, Kei Yura, Masaaki Umeda, Vicente Rubio, Yasuomi Tada, Tsuyoshi Furumoto, Takashi Aoyama, Tomohiko Tsuge
Summary: This study found that the plant CFI 25 subunit plays an important role in maintaining the diversity of mRNA 3' ends. Multiple forms of CFI exist in plants, and AtCFI 25a is essential for maintaining the proper diversity of 3' end lengths of transcripts coding for CFI subunits and plays important roles in Arabidopsis development.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jeong Hun Kim, Han Nim Lee, Xiao Huang, Hyera Jung, Marisa S. Otegui, Faqiang Li, Taijoon Chung
Summary: The plant-specific PI3P-binding protein FYVE2 provides a functional link between the COPII machinery and autophagy by interacting with the small GTPase SAR1 and the PI3P effector ATG18A. FYVE2 regulates autophagosome biogenesis and delivery to vacuoles, working downstream of ATG2. Mutations in FYVE2 or dominant-negative mutant SAR1B proteins result in reduced autophagic flux and organelle accumulation.
Review
Biochemistry & Molecular Biology
Byung-Ho Kang, Charles T. Anderson, Shin-ichi Arimura, Emmanuelle Bayer, Magdalena Bezanilla, Miguel A. Botella, Federica Brandizzi, Tessa M. Burch-Smith, Kent D. Chapman, Kai Duenser, Yangnan Gu, Yvon Jaillais, Helmut Kirchhoff, Marisa S. Otegui, Abel Rosado, Yu Tang, Juergen Kleine-Vehn, Pengwei Wang, Bethany Karlin Zolman
Summary: The article summarizes a modern view of plant cell structures and unresolved mysteries, discussing the complexities of internal structures and the shapes revealed by new microscopy techniques. Future research will continue to focus on the combination of imaging modalities with functional studies.
Review
Biochemistry & Molecular Biology
Adrienne H. K. Roeder, Marisa S. Otegui, Ram Dixit, Charles T. Anderson, Christine Faulkner, Yan Zhang, Maria J. Harrison, Charlotte Kirchhelle, Gohta Goshima, Jeremy E. Coate, Jeff J. Doyle, Olivier Hamant, Keiko Sugimoto, Liam Dolan, Heather Meyer, David W. Ehrhardt, Arezki Boudaoud, Carlos Messina
Summary: The open questions in plant cell biology raised by 15 experts cover a wide range of topics, including organelle maintenance, cell wall synthesis, cell division, polyploidy effects, intracellular signaling, and emergent effects of complex dynamical processes on cells, organs, and organisms.
Article
Plant Sciences
Laura Cuyas, Lun Jing, Sylvain Pluchon, Mustapha Arkoun
Summary: The study showed that under conditions of external phosphate deprivation, the addition of silicon can promote wheat plants to recycle phosphate from phospho-metabolites, leading to increased phosphate absorption and utilization efficiency.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2022)
Article
Biology
Francesco Padovani, Benedikt Mairhoermann, Pascal Falter-Braun, Jette Lengefeld, Kurt M. Schmoller
Summary: Cell-ACDC is an open-source user-friendly framework written in Python for segmentation, tracking, and cell cycle annotations in live cell imaging data. It includes state-of-the-art deep learning models, visualization and error correction tools, and allows for fast integration of new methods.
Article
Plant Sciences
Laura Cuyas, Lun Jing, Sylvain Pluchon, Mustapha Arkoun
Summary: Wheat plants were treated with three different levels of phosphate deficiency to study the metabolic adaptations and differences in metabolites. It was found that carbohydrate and amino acid metabolism were more affected in shoots, while secondary metabolism was more affected in roots in both types of phosphate deficiency. Several metabolites highly sensitive to phosphate deficiency were also identified. These findings provide new insights into wheat's metabolic pathways under phosphate deficiency and contribute to better handling of future phosphate scenarios.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2023)
Article
Plant Sciences
Jessica A. S. Barros, Elizabeth C. Chatt, Robert C. Augustine, Fionn McLoughlin, Faqiang Li, Marisa S. Otegui, Richard D. Vierstra
Summary: Autophagy plays a minor role in starch and protein accumulation during maize endosperm development, but helps protect against oxidative stress and clear dysfunctional mitochondria.
Article
Biology
Han Nim Lee, Jenu Varghese Chacko, Ariadna Gonzalez Solis, Kuo-En Chen, Jessica A. S. Barros, Santiago Signorelli, A. Harvey Millar, Richard David Vierstra, Kevin W. Eliceiri, Marisa S. Otegui, Yoselin Benitez-Alfonso
Summary: This study reveals a new function of NBR1 in the degradation of damaged chloroplasts through microautophagy, where NBR1 associates with photodamaged chloroplasts independently of ATG7 and delivers them to vacuoles for degradation.
Article
Plant Sciences
Laura Cuyas, Pascale David, Damien de Craieye, Sophia Ng, Mustapha Arkoun, Claude Plassard, Mohamadi Faharidine, Delphine Hourcade, Francesca Degan, Sylvain Pluchon, Laurent Nussaume
Summary: By conducting transcriptomic studies in the model plant Arabidopsis thaliana, we identified conserved molecular markers for Pi starvation in early roots or leaves that respond rapidly to changes in phosphate nutritional status. We found their homologues in three crops and demonstrated their reliability in monitoring the plant's internal Pi status. These markers provide valuable tools for plant physiologists and breeders to assess the impact of phosphate bio-availability on plant growth.
Article
Multidisciplinary Sciences
Florin Ratajczak, Mitchell Joblin, Marcel Hildebrandt, Martin Ringsquandl, Pascal Falter-Braun, Matthias Heinig
Summary: Understanding phenotype-genotype relationships is a challenge in biology, and the authors use graph representation learning to identify human genes with core gene characteristics for complex diseases. The core-like genes exhibit similar properties to core genes and are attractive targets for drug development.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Chan Liu, Zhimin Li, Dan Tian, Mei Xu, Jianwei Pan, Haijun Wu, Chao Wang, Marisa S. Otegui
Summary: The Arabidopsis AP1/2 beta adaptins play a critical role in plants, regulating the formation of the pollen wall and reproduction by mediating the trafficking of ABC transporters during pollen development.
Article
Biochemistry & Molecular Biology
Lei Li, Chun Pong Lee, Xinxin Ding, Yu Qin, Akila Wijerathna-Yapa, Martyna Broda, Marisa S. Otegui, A. Harvey Millar
Summary: Protein cargo of autophagy in plants, specifically ATG11 and ATG5, play a crucial role in the degradation of specific enzymes and proteins in various organelles. Autophagy is also involved in metabolic changes and protein degradation rates under phosphate limitation, and specifically targets proteins involved in chloroplast autophagy and secondary metabolism.
Correction
Biochemistry & Molecular Biology
Ximena Cardona-Lopez, Laura Cuyas, Elena Marin, Charukesi Rajulu, Maria Luisa Irigoyen, Erica Gil, Maria Isabel Puga, Richard Bligny, Laurent Nussaume, Niko Geldner, Javier Paz-Ares, Vicente Rubio