Review
Environmental Sciences
Ericka Marel Quezada-Maldonado, Yesennia Sanchez-Perez, Yolanda I. Chirino, Claudia M. Garcia-Cuellar
Summary: This study analyzed the types of DNA damage and alterations in DNA repair pathways induced by PM exposure, showing that PM mainly causes oxidative stress and DNA damage through the formation of DNA adducts and DSBs, as well as deregulates the protein expression in DNA repair pathways. However, there are still limitations in the knowledge about the effects of PM on DNA repair pathways, and further research is needed to deepen our understanding.
ENVIRONMENTAL POLLUTION
(2021)
Article
Multidisciplinary Sciences
Diana Rubio-Contreras, Fernando Gomez-Herreros
Summary: This study reveals that double strand breaks (DSBs) induced by DNA topoisomerase I (TOP1) can lead to genome rearrangements, which are repaired by tyrosyl-DNA phosphodiesterase 1 (TDP1), thereby protecting gene transcription and genome stability.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Michelle L. Swift, Kate Beishline, Samuel Flashner, Jane Azizkhan-Clifford
Summary: This passage discusses the role of Sp1 in the cell cycle and its regulation of DSB repair pathway choice, favoring NHEJ repair. Sp1 is shown to recruit the NHEJ repair factor 53BP1 in G1 phase, while being evicted through phosphorylation in S phase, inhibiting HR repair.
Article
Cell Biology
Md Akram Hossain, Yunfeng Lin, Garrett Driscoll, Jia Li, Anne McMahon, Joshua Matos, Haichao Zhao, Daisuke Tsuchimoto, Yusaku Nakabeppu, Jianjun Zhao, Shan Yan
Summary: APE2 is essential for activating the ATR DDR pathway in response to various stressful conditions in Xenopus laevis egg extracts and human pancreatic cancer cells. Inhibition of APE2 leads to increased DNA damage and sensitizes cancer cells to chemotherapy drugs, indicating its crucial role in maintaining genome integrity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Ksenia G. Kolobynina, Alexander Rapp, M. Cristina Cardoso
Summary: Chromatin serves as the background for all DNA-based molecular processes in the cell nucleus. The initial chromatin structure at the site of DNA damage determines lesion generation and activation of the DNA damage response pathway. Ubiquitination, as an important chromatin post-translational modification, is involved in chromatin changes at the damaged site and throughout the genome.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Watanya Trakarnphornsombat, Hiroshi Kimura
Summary: DNA double-strand breaks (DSBs) can cause genetic mutation and histone H2AX is phosphorylated by kinases such as ATM, ATR, and DNA-PK upon DSB induction. The accumulation kinetics of ?-H2AX were similar in ATM-proficient and-deficient cells, but delayed in the presence of a DNA-PK inhibitor. Ku80 (XRCC5) diffuses freely in the nucleus, while ATM repeatedly binds to and dissociates from chromatin. ATM accumulation at damage sites is regulated by the histone acetyltransferase MOF, but does not necessarily reflect in the ?-H2AX level, suggesting distinct actions of ATM and DNA-PK in immediate ?-H2AX accumulation.
JOURNAL OF CELL SCIENCE
(2023)
Article
Cell Biology
Nathalie Berthault, Ptissam Bergam, Floriane Pereira, Pierre-Marie Girard, Marie Dutreix
Summary: AsiDNA, a DNA repair inhibitor, disrupts DNA double-strand breaks (DSB) repair pathways to sensitize tumors to DNA damaging therapies. It activates ATM and PARP in the cytoplasm, preventing the formation of repair foci on irradiation-induced damage. AsiDNA also associates with DNA-PK in the nucleus to inhibit the recruitment of repair enzymes at damage sites.
Article
Biochemistry & Molecular Biology
Almudena Serrano-Benitez, Sophie E. Wells, Lylah Drummond-Clarke, Lilian C. Russo, John Christopher Thomas, Giovanna A. Leal, Mark Farrow, James Michael Edgerton, Shankar Balasubramanian, Ming Yang, Christian Frezza, Amit Gautam, Jan Brazina, Kamila Burdova, Nicolas C. Hoch, Stephen P. Jackson, Keith W. Caldecott
Summary: DNA single-strand breaks (SSBs) play a role in disrupting DNA replication and causing chromosome breakage. This study investigates whether SSBs induce chromosome breakage when located behind or ahead of replication forks, and finds that only SSBs ahead of replication forks trigger fork collapse and chromosome breakage. Furthermore, the study shows that CldU, a thymidine analogue, is cytotoxic to cells lacking SSB repair mechanisms and its incorporation in template DNA is particularly harmful during the following cell cycle. Additionally, BRCA-defective cells are highly sensitive to CldU, suggesting its potential clinical utility.
Article
Biochemistry & Molecular Biology
Aaron Mendez-Bermudez, Liudmyla Lototska, Melanie Pousse, Florent Tessier, Oliver Croce, Chrysa M. Latrick, Veronica Cherdyntseva, Joe Nassour, Jiang Xiaohua, Yiming Lu, Corinne Abbadie, Sarantis Gagos, Jing Ye, Eric Gilson
Summary: Cellular senescence triggers various types of heterochromatin remodeling, including decondensation, DNA damage, and illegitimate recombination. In this study, it was discovered that at the onset of senescence, pericentromeric heterochromatin is specifically dismantled due to telomere shortening or genotoxic stress. This process is initiated by the TP53-TRF2 axis and involves the downregulation of TRF2, resulting in heterochromatin decondensation and DNA breaks.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Xiaoqing Li, Dexuan Gao, Fei Shen, Hengrui Chen, Zhuqiang Zhang, Chao He, Aidi Gao, Yue Lang, Xiaozhong Zhu, Jundong Zhou, Zeng-Fu Shang, Wei-Qun Ding, Ji Zhu
Summary: Radiotherapy resistance is a crucial challenge in managing esophageal squamous carcinoma (ESCC), but the factors and molecular mechanisms underlying ESCC resistance to radiotherapy are not fully understood. This study found that high expression of DNA polymerase iota (POLI) is associated with shorter survival in ESCC patients receiving radiotherapy. Down-regulating POLI increased ESCC sensitivity to radiation and prolonged DNA damage markers. POLI stabilizes RAD51 protein by competitively binding with E3 ligase XIAP and blocking RAD51's ubiquitination, leading to the activation of the GAS signaling pathway. These findings provide new insights into the role of POLI in ESCC radioresistance by stabilizing RAD51 protein.
CELL DEATH DISCOVERY
(2023)
Article
Cell Biology
Vera Chesnokova, Svetlana Zonis, Athanasia Apostolou, Hannah Q. Estrada, Simon Knott, Kolja Wawrowsky, Kathrin Michelsen, Anat Ben-Shlomo, Robert Barrett, Vera Gorbunova, Katia Karalis, Shlomo Melmed, Kolja Wawrowsky, Kathrin Michelsen, Anat Ben-Shlomo, Robert Barrett, Vera Gorbunova, Katia Karalis, Shlomo Melmed
Summary: The study found that as individuals age, the level of non-pituitary growth hormone (npGH) in the body increases, leading to DNA damage accumulation. npGH can suppress p53 and weaken DNA repair response, accelerating DNA damage. Inhibiting npGH signaling could be a potential anti-aging therapy strategy.
Article
Biochemistry & Molecular Biology
Laurene Sonzogni, Melanie L. Ferlazzo, Adeline Granzotto, Beatrice Fervers, Laurent Charlet, Nicolas Foray
Summary: The mechanistic model RIANS from radiobiology is crucial for understanding the recognition, repair and genotoxic stress response of DNA double-strand breaks induced by radiation. This model also applies to exposure to metal ions. Our study found that the induction of DSB by pesticides depends on their concentration and the RIANS status of cells. Impaired DSB recognition and repair, leading to toxicity, can occur when the nucleo-shuttling of ATM is delayed. Additionally, the combination of certain metal ions and pesticides can have additive or supra-additive effects on DSB recognition and/or repair.
Article
Biochemistry & Molecular Biology
Vandana Singh, Pegah Johansson, Elina Ekedahl, Yii-Lih Lin, Ola Hammarsten, Fredrik Westerlund
Summary: In this study, single DNA molecule imaging using fluorescence microscopy was used to quantify DNA damage caused by the topoisomerase II poison etoposide. The researchers found a large variation in etoposide-induced DNA damage after in vitro treatment of blood cells from healthy individuals. The results further demonstrated that the etoposide-induced DNA damage was TopoII dependent.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Zhong-Xuan Wang, Yi Liu, Yao-Lin Li, Qiao Wei, Rong-Rong Lin, Ruiqing Kang, Yang Ruan, Zhi-Hao Lin, Nai-Jia Xue, Bao-Rong Zhang, Jia-Li Pu
Summary: DNA damage and defective DNA repair are implicated in neurodegeneration of Parkinson's disease. The study shows that the protein DJ-1 plays a crucial role in modulating DNA double-strand break repair by promoting both homologous recombination and nonhomologous end joining. DJ-1 interacts with PARP1 and enhances its enzymatic activity during DNA repair. Importantly, PD patients with DJ-1 mutation have impaired PARP1 activity and DSB repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Kai Zhang, Qingnan Wu, Wenzhong Liu, Yan Wang, Lianmei Zhao, Jie Chen, Haoyu Liu, Siqi Liu, Jinting Li, Weimin Zhang, Qimin Zhan
Summary: This study identified a novel DDR regulator, FAM135B, which plays a crucial role in maintaining genomic integrity and promoting DNA repair. The results demonstrated that FAM135B can enhance homologous recombination and non-homologous end-joining repairs, as well as improve DNA damage response by physically binding to TIP60 and enhancing its activity.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
S. V. Khoronenkova
BIOCHEMISTRY-MOSCOW
(2016)
Article
Biochemistry & Molecular Biology
N. V. Komarova, I. V. Golubev, S. V. Khoronenkova, T. A. Chubar, V. I. Tishkov
BIOCHEMISTRY-MOSCOW
(2012)
Article
Developmental Biology
Paraskevi Goggolidou, Jonathan L. Stevens, Francesco Agueci, Jennifer Keynton, Gabrielle Wheway, Daniel T. Grimes, Saloni H. Patel, Helen Hilton, Stine K. Morthorst, Antonella DiPaolo, Debbie J. Williams, Jeremy Sanderson, Svetlana V. Khoronenkova, Nicola Powles-Glover, Alexander Ermakov, Chris T. Esapa, Rosario Romero, Grigory L. Dianov, James Briscoe, Colin A. Johnson, Lotte B. Pedersen, Dominic P. Norris
Article
Biochemistry & Molecular Biology
Jason L. Parsons, Irina I. Dianova, Svetlana V. Khoronenkova, Mariola J. Edelmann, Benedikt M. Kessler, Grigory L. Dianov
Article
Biochemistry & Molecular Biology
Svetlana V. Khoronenkova, Irina I. Dianova, Nicola Ternette, Benedikt M. Kessler, Jason L. Parsons, Grigory L. Dianov
Article
Biochemistry & Molecular Biology
Svetlana V. Khoronenkova, Irina I. Dianova, Jason L. Parsons, Grigory L. Dianov
NUCLEIC ACIDS RESEARCH
(2011)
Article
Biochemistry & Molecular Biology
Elisa Zucca, Federica Bertoletti, Ursula Wimmer, Elena Ferrari, Giuliano Mazzini, Svetlana Khoronenkova, Nicole Grosse, Barbara van Loon, Grigory Dianov, Ulrich Huebscher, Giovanni Maga
NUCLEIC ACIDS RESEARCH
(2013)
Article
Biochemistry & Molecular Biology
Svetlana V. Khoronenkova, Grigory L. Dianov
NUCLEIC ACIDS RESEARCH
(2013)
Article
Biochemistry & Molecular Biology
Jason L. Parsons, Svetlana V. Khoronenkova, Irina I. Dianova, Nicola Ternette, Benedikt M. Kessler, Pran K. Datta, Grigory L. Dianov
NUCLEIC ACIDS RESEARCH
(2012)
Article
Biochemistry & Molecular Biology
Giulia Orlando, Svetlana V. Khoronenkova, Irina I. Dianova, Jason L. Parsons, Grigory L. Dianov
NUCLEIC ACIDS RESEARCH
(2014)
Article
Chemistry, Multidisciplinary
N. V. Komarova, I. V. Golubev, S. V. Khoronenkova, V. I. Tishkov
RUSSIAN CHEMICAL BULLETIN
(2012)
Article
Oncology
Garth Hamilton, Aswin G. Abraham, Jennifer Morton, Oliver Sampson, Dafni E. Pefani, Svetlana Khoronenkova, Anna Grawenda, Angelos Papaspyropoulos, Nigel Jamieson, Colin McKay, Owen Sansom, Grigory L. Dianov, Eric O'Neill
Article
Biochemistry & Molecular Biology
Julie Bourseguin, Wen Cheng, Emily Talbot, Liana Hardy, Jenny Lai, Ailsa M. Jeffries, Michael A. Lodato, Eunjung Alice Lee, Svetlana Khoronenkova
Summary: ATM deficiency in Ataxia-telangiectasia can result in DNA damage and dysfunction of immune cell microglia, leading to neurodegeneration.
NUCLEIC ACIDS RESEARCH
(2022)
Meeting Abstract
Genetics & Heredity
Svetlana Khoronenkova, Grigory Dianov