Article
Cell Biology
Lei Li, Haowen Liu, Mia Krout, Janet E. Richmond, Yu Wang, Jihong Bai, Saroja Weeratunga, Brett M. Collins, Donovan Ventimiglia, Yi Yu, Jingyao Xia, Jing Tang, Jie Liu, Zhitao Hu
Summary: This study identifies a dual Ca2+ sensor system in C. elegans, with SNT-1 and SNT-3 triggering neurotransmitter release at different rates. While SNT-1 is tethered to synaptic vesicles, SNT-3 functions independently of vesicles.
JOURNAL OF CELL BIOLOGY
(2021)
Editorial Material
Cell Biology
Ok-Ho Shin, Ege T. Kavalali
Summary: Research has identified multiple proteins containing C2 domains as Ca2+ sensors in neurotransmitter release, where they function together to sustain different forms of release. A novel Ca2+ sensor has been identified in C. elegans, complementing the role of synaptotagmin-1 in mediating slower evoked release. Earlier studies also suggest an evolutionarily conserved diversity of Ca2+ sensors mediating distinct forms of neurotransmitter release.
Article
Neurosciences
Boris Bouazza-Arostegui, Marcial Camacho, Marisa M. Brockmann, Sina Zobel, Christian Rosenmund
Summary: SYT1 triggers neurotransmitter release by interacting with anionic lipids in a calcium-dependent manner and negatively regulates spontaneous release; its relationship with other SYT isoforms is under investigation; in exocytosis, release probability is most sensitive to variation in SYT1 expression levels, while vesicle priming is least sensitive.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Takaaki Fujii, Akira Sakurai, J. Troy Littleton, Motojiro Yoshihara
Summary: Short-term synaptic plasticity, a rapid and robust modification in neuronal pre-synaptic output, is determined by the Ca2+-binding protein Synaptotagmin 7 (Syt7). Syt7 controls the initial probability of synaptic vesicle fusion to determine whether neurons display short-term facilitation or depression. Syt7 acts to suppress synaptic transmission in order to maintain an output range where facilitation is available to the neuron.
SCIENTIFIC REPORTS
(2021)
Article
Neurosciences
Michael J. Seibert, Chantell S. Evans, Kevin S. Stanley, Zhenyong Wu, Edwin R. Chapman
Summary: SYT9 is a Ca2+ sensor in neuroendocrine cells, but its function in neurons is unclear. In this study, it was found that SYT9 does not trigger rapid synaptic vesicle exocytosis in mouse cortical, hippocampal, or striatal neurons unless it is overexpressed. Loss of SYT9 in striatal neurons reduced the frequency of spontaneous neurotransmitter release events. Further investigation revealed that SYT9 is localized to dense-core vesicles containing substance P, and loss of SYT9 impaired SP release, causing the observed decrease in mini frequency. The study also showed that Ca2+ binding to the C2A domain of SYT9 triggered membrane fusion in vitro, and mutations disrupting this activity abolished SYT9's ability to regulate SP release and mini frequency. Therefore, it can be concluded that SYT9 indirectly regulates synaptic transmission in striatal neurons by controlling SP release.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Yuni Kay, Bruce E. Herring
Summary: Efficient methods are lacking for studying proteins regulating presynaptic function, but the present study introduces an optical/electrophysiological method that utilizes optogenetics and simultaneous recordings of fiber volley amplitude to quantify synaptic efficacy. By inhibiting synaptotagmin 1 expression in CA3 pyramidal neurons, the study demonstrates a significant reduction in Schaffer collateral synapse function. This method aims to expedite understanding of molecular regulatory pathways governing presynaptic function.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Matteo Moschetta, Tiziana Ravasenga, Antonio De Fusco, Luca Maragliano, Davide Aprile, Marta Orlando, Silvio Sacchetti, Silvia Casagrande, Gabriele Lignani, Anna Fassio, Pietro Baldelli, Fabio Benfenati
Summary: The study reveals that Synapsin I acts as a novel Ca2+ buffer in excitatory terminals, and blocking its binding to Ca2+ leads to abnormal neurotransmitter release and short-term plasticity. This finding sheds light on the physiological role of Ca2+ binding to SynI in the regulation of SV clustering and trafficking in nerve terminals.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Neurosciences
Zhenyong Wu, Lu Ma, Nicholas A. Courtney, Jie Zhu, Ane Landajuela, Yongli Zhang, Edwin R. Chapman, Erdem Karatekin
Summary: This study aims to explore the critical features of the C2A domain of Syt1 and compare it to the analogous feature in C2B. The research found that both the poly-lysine patches in C2A and C2B contribute to membrane binding, and both are crucial for evoked release.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Xiling Li, Chun Chien, Yifu Han, Zihan Sun, Xun Chen, Dion Dickman
Summary: The study revealed that the Glutamate-gated chloride channel (GluCl alpha) in Drosophila neurons plays a critical role in presynaptic homeostatic depression (PHD), driving the process through an activity-dependent anionic conductance.
Article
Multidisciplinary Sciences
Ryota Fukaya, Marta Maglione, Stephan J. Sigrist, Takeshi Sakaba
Summary: This study suggests a potential mechanism for the cAMP-dependent increase in neurotransmission at hippocampal mossy fiber-CA3 synapses, involving an accumulation of active zone Ca2+ channels. Experimental results indicate an increase in the local Ca2+ concentration at the release site following potentiation, driven by rapid Ca2+ channel accumulation, rather than changes in intracellular Ca2+ sensitivity. Various microscopy techniques provide insights into the underlying mechanisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Neurosciences
Chuangeng Zhang, Meijian Wang, Shengyin Lin, Ruili Xie
Summary: The study reveals that Calretinin is selectively expressed in different subtypes, affecting synaptic efficacy and recovery speed, contributing to reduce calcium accumulation during high-rate activity. Synapses with differential CR expression exhibit differences in synaptic transmission and VGluT expression, influencing their ability to transmit auditory information.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Biology
Janus R. L. Kobbersmed, Manon M. M. Berns, Susanne Ditlevsen, Jakob B. Sorensen, Alexander M. Walter
Summary: Synaptic communication relies on the fusion of synaptic vesicles with the plasma membrane, which is achieved through the allosteric stabilization of synaptotagmin's Ca2+/PI(4,5)P-2 dual-bound state and the coordinated function of multiple synaptotagmins.
Article
Multidisciplinary Sciences
Philipe R. F. Mendonca, Erica Tagliatti, Helen Langley, Dimitrios Kotzadimitriou, Criseida G. Zamora-Chimal, Yulia Timofeeva, Kirill E. Volynski
Summary: This study reveals the variation in synchronous and asynchronous release ratio among synaptic outputs from single pyramidal cells, and demonstrates that low release probability synapses have reduced synchronicity. Additionally, the distribution of asynchronous release sites is more widespread within the release area.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Quentin Bourgeois-Jaarsma, Pablo Miaja Hernandez, Alexander J. Groffen
Summary: Presynaptic neurotransmitter release is strictly regulated by SNARE proteins, Ca2+ and a number of Ca2+ sensors. Proteins like Syt-1, Syt7 and Doc2 contribute predominantly to different phases of release, showing functional diversity in their subcellular location, Ca2+-binding properties and protein interactions. Studies suggest a possible regulatory role of rabphilin-3a in neuronal networks, narrowing down the repertoire of synaptic Ca2+ sensors involved in the spontaneous release of glutamate.
MOLECULAR AND CELLULAR NEUROSCIENCE
(2021)
Article
Cell Biology
Meida Jusyte, Natalie Blaum, Mathias A. Boehme, Manon M. M. Berns, Alix E. Bonard, Abel B. Vamosi, Kavya V. Pushpalatha, Janus R. L. Kobbersmed, Alexander M. Walter
Summary: Presynaptic plasticity adjusts neurotransmitter (NT) liberation, with short-term facilitation (STF) and presynaptic homeostatic potentiation (PHP) playing roles in millisecond repetitive activation and stabilizing transmission over minutes, respectively. Functional overlap and shared molecular dependence on Unc13A's release-site protein are observed despite different timescales of STF and PHP. Mutating Unc13A's calmodulin binding domain (CaM-domain) blocks STF and PHP while increasing baseline transmission, suggesting a role for the Ca2+/calmodulin/Unc13A interaction in plastic stabilization of vesicle priming. Acute phorbol ester treatment enhances NT release and blocks STF/PHP in synapses expressing wild-type Unc13A but not in synapses with CaM-domain mutation, indicating common downstream effects. The regulatory domains of Unc13A integrate signals across timescales to regulate release-site participation for synaptic plasticity.
Article
Biochemistry & Molecular Biology
Zack Zurawski, Brian Page, Michael C. Chicka, Rebecca L. Brindley, Christopher A. Wells, Anita M. Preininger, Karren Hyde, James A. Gilbert, Osvaldo Cruz-Rodriguez, Kevin P. M. Currie, Edwin R. Chapman, Simon Alford, Heidi E. Hamm
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Physiology
Tejeshwar C. Rao, Zuleirys Santana Rodriguez, Mazdak M. Bradberry, Alexandra H. Ranski, Peter J. Dahl, Michael W. Schmidtke, Paul M. Jenkins, Daniel Axelrod, Edwin R. Chapman, David R. Giovannucci, Arun Anantharam
JOURNAL OF GENERAL PHYSIOLOGY
(2017)
Article
Cell Biology
Berrak Ugur, Huan Bao, Michal Stawarski, Lita R. Duraine, Zhongyuan Zuo, Yong Qi Lin, G. Gregory Neely, Gregory T. Macleod, Edwin R. Chapman, Hugo J. Bellen
Article
Cell Biology
Mounir Bendahmane, Kevin P. Bohannon, Mazdak M. Bradberry, Tejeshwar C. Rao, Michael W. Schmidtke, Prabhodh S. Abbineni, Nara L. Chon, Sherleen Tran, Hai Lin, Edwin R. Chapman, Jefferson D. Knight, Arun Anantharam
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Neurosciences
Nicholas A. Courtney, Joseph S. Briguglio, Mazdak M. Bradberry, Christina Greer, Edwin R. Chapman
Article
Multidisciplinary Sciences
Renhao Xue, David A. Ruhl, Joseph S. Briguglio, Alexander G. Figueroa, Robert A. Pearce, Edwin R. Chapman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Editorial Material
Neurosciences
Edwin R. Chapman
TRENDS IN NEUROSCIENCES
(2018)
Article
Multidisciplinary Sciences
Huan Bao, Debasis Das, Nicholas A. Courtney, Yihao Jiang, Joseph S. Briguglio, Xiaochu Lou, Daniel Roston, Qiang Cui, Baron Chanda, Edwin R. Chapman
Article
Cell Biology
Erin L. Slosarek, Amber L. Schuh, Iryna Pustova, Adam Johnson, Jennifer Bird, Matthew Johnson, E. B. Frankel, Nilakshee Bhattacharya, Michael G. Hanna, Jordan E. Burke, David A. Ruhl, Kyle Quinney, Samuel Block, Jennifer L. Peotter, Edwin R. Chapman, Michael D. Sheets, Samuel E. Butcher, Scott M. Stagg, Anjon Audhya
Article
Biochemical Research Methods
Jonathan S. Marvin, Benjamin Scholl, Daniel E. Wilson, Kaspar Podgorski, Abbas Kazemipour, Johannes Alexander Mueller, Susanne Schoch, Francisco Jose Urra Quiroz, Nelson Rebola, Huan Bao, Justin P. Little, Ariana N. Tkachuk, Edward Cai, Adam W. Hantman, Samuel S. -H. Wang, Victor J. DePiero, Bart G. Borghuis, Edwin R. Chapman, Dirk Dietrich, David A. DiGregorio, David Fitzpatrick, Loren L. Looger
Article
Cell Biology
Zhiyi Hu, Bailian Cai, Mengfei Wang, Xiaoli Wen, Anke Geng, Xiang Hu, Renhao Xue, Zhiyong Mao, Ying Jiang, Xiaoping Wan
Article
Cell Biology
Weina Zhang, Jiaqing Yang, Yu Chen, Renhao Xue, Zhiyong Mao, Wen Lu, Ying Jiang
Summary: Lycorine hydrochloride can significantly suppress cellular premature senescence, promote DNA double-strand break repair pathways, and stabilize genomes by enhancing the transcription of SIRT1 and SIRT6.
Review
Neurosciences
Renhao Xue, Hao Meng, Jiaxiang Yin, Jingyao Xia, Zhitao Hu, Huisheng Liu
Summary: Exocytosis is a Ca2+-regulated process that involves the participation of Ca2+ sensors, with synaptotagmin acting as the primary sensor in the final stage of membrane fusion triggered by a Ca2+ boost. Calmodulin, on the other hand, plays a role in the earlier exocytotic steps by acting as a high affinity Ca2+ sensor. Both calmodulin and synaptotagmin play complementary roles throughout the exocytosis process.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Cell Biology
Nan Qin, Zhixi Chen, Renhao Xue
Summary: Exocytosis of large dense core vesicles is crucial for hormone secretion in neuroendocrine cells. This study develops a novel model based on single vesicle release events to describe the architecture of the releasable vesicle pool and elucidate the heterogeneity of the vesicle priming mechanism.