Article
Biochemistry & Molecular Biology
Njikem Asong-Fontem, Arnau Panisello-Rosello, Mylene Sebagh, Mathilde Gonin, Joan Rosello-Catafau, Rene Adam
Summary: The scarcity of livers for transplantation is a growing concern and researchers are exploring new strategies to expand the donor pool. A study found that IGL-2 preservation solution demonstrated a protective role for fatty livers by preserving endothelial glycocalyx, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Anna Radajewska, Anna Krzywonos-Zawadzka, Iwona Bil-Lula
Summary: Kidney transplantation is the standard procedure for treating end-stage renal disease, and machine perfusion has been proven to be a better solution for organ preservation and monitoring.
Review
Medicine, General & Internal
Ahmed S. Ghoneima, Richard X. Sousa X. Da Silva, Martina A. Gosteli, Adam D. Barlow, Philipp Kron
Summary: The high demand for organs in kidney transplantation has led to the widespread use of machine perfusion technologies. This study provides a systematic review of the developments in this field over the past 10 years and compares different perfusion techniques. The results show that dynamic preservation strategies, such as hypothermic machine perfusion, have lower rates of delayed graft function and have the potential to improve outcomes in kidney transplantation.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Urology & Nephrology
Asel S. Arykbaeva, Dorottya K. de Vries, Jason B. Doppenberg, Marten A. Engelse, Thomas Hankemeier, Amy C. Harms, Leonie G. Wijermars, Alexander F. Schaapherder, Jaap A. Bakker, Rutger J. Ploeg, Ian P. J. Alwayn, Jan H. N. Lindeman
Summary: Normothermic machine perfusion (NMP) is a novel preservation strategy that maintains organs in a metabolically active state, providing superior organ preservation and allowing viability testing before transplantation. However, current perfusion protocols may not fully address the metabolic requirements of marginal donor grafts. Monitoring of metabolic status during NMP is important for optimal outcomes.
KIDNEY INTERNATIONAL
(2021)
Review
Biochemistry & Molecular Biology
Tom Darius, Jay Nath, Michel Mourad
Summary: The use of high-risk renal grafts for transplantation requires optimization of pretransplant preservation and assessment strategies to improve clinical outcomes and decrease organ discard rate. Oxygenation during hypothermic machine preservation is proposed as a resuscitative measure, providing a critical overview of the fundamentals and suggesting different strategies for clinical implementation.
Review
Medicine, Research & Experimental
Elisa B. Monteiro, Matheus Ajackson, Milena B. Stockler-Pinto, Fitsum Guebre-Egziabher, Julio B. Daleprane, Christophe O. Soulage
Summary: This review analyzed the renoprotective effects of sulforaphane (SFN) in preclinical models of kidney diseases. The results showed that SFN improved renal function, reduced kidney injury, and decreased urinary protein excretion and kidney fibrosis. These findings suggest that SFN supplementation may be a potential therapeutic strategy for kidney disease.
Article
Physiology
Rosa G. M. Lammerts, Lisanne M. Lagendijk, Gesa Tiller, Wendy A. Dam, Harriet L. Lancaster, Mohamed R. Daha, Marc A. Seelen, Bouke G. Hepkema, Robert A. Pol, Henri G. D. Leuvenink, Grietje Molema, Jacob van den Born, Stefan P. Berger
Summary: Renal endothelial cells play important roles in renal diseases, and a new method of isolating ECs from the perfusate of donor kidneys has been described in this study, demonstrating potential diagnostic value in HLA antibody-specific cross-matching tests and further applications in renal endothelial research.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yuan Meng Yu, Wei Wang, Jiqiu Wen, Yong Zhang, Guang Ming Lu, Long Jiang Zhang
Summary: ASL was found to be more promising than IVIM DWI in distinguishing renal allograft fibrosis degree and predicting long-term allograft dysfunction.
EUROPEAN RADIOLOGY
(2021)
Review
Medicine, General & Internal
Natalia Warmuzinska, Kamil Luczykowski, Barbara Bojko
Summary: The number of patients on kidney transplant waiting lists is increasing, resulting in a gap between organ demand and availability. To address this shortage, medical professionals have started using marginal kidneys from expanded criteria donors (ECD). However, recipients of ECD kidneys tend to have worse outcomes compared to those receiving organs from standard criteria donors (SCD). Therefore, there is a need for representative methods to assess the quality of these transplant grafts. Current evaluation methods are limited and include interpreting laboratory tests, clinical risk scores, visual evaluation of the organ, and in some cases, a biopsy and perfusion parameters. New technologies have emerged in recent years to examine organ function and understand the mechanisms associated with complications. This review summarizes current diagnostic methods and new potential strategies for graft-quality assessment, identification of complications, perfusion control, and prediction of outcomes.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Eileen Edgworth, Lisa Ernst, Zoltan Czigany, Turgay Saritas, Laura Sophie Zarnitz, Marc Wiartalla, Peter Boor, Eva Miriam Buhl, Rolf Rossaint, Rene H. Tolba, Benedict Doorschodt, Gregor Fabry, Christian Bleilevens
Summary: Normothermic machine perfusion (NMP) combined with optimized perfusate composition can improve the quality of kidney preservation, but the optimization of perfusates is still under research. Hemoglobin-based oxygen carriers (HBOCs), such as HBOC-301, can be an alternative to packed red blood cells (pRBC), but they can cause methemoglobin formation and induce hypoxia. The addition of ascorbic acid (VitC) as an antioxidant may have a beneficial effect. This study showed that HBOC-301 is inferior to pRBCs in a porcine kidney NMP model, regardless of the addition of VitC. Oxidative stress and fragmentation of hemoglobin polymers may contribute to these results, indicating the need for further research on cell-free oxygen carriers without these issues.
Review
Gastroenterology & Hepatology
Bianca Della Guardia, Amanda P. C. S. Boteon, Celso E. L. Matielo, Guilherme Felga, Yuri L. Boteon
Summary: Acute-on-chronic liver failure (ACLF) is a common syndrome characterized by acute decompensation, organ failure, and high short-term mortality. Liver transplantation is an effective protective measure, and providing supportive treatment is crucial. However, more research and global discussion are needed to improve ACLF management.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Review
Surgery
Laurence Verstraeten, Ina Jochmans
Summary: This article discusses the use of organ perfusion technology as a tool for assessing organ viability before transplantation, highlighting current issues, potential developments, and the pros and cons of various perfusion methods.
TRANSPLANT INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Jianbo Li, XiangJun Zha, Yan Kang, Zhongwei Zhang, Lvnan Yan, Lujia Song, Chengdi Wang, Jiayin Yang
Summary: A proposed oxygen-carrying sequential preservation (OCSP) method combining oxygenated static cold storage (SCS) and normothermic mechanical perfusion was found to decrease ischemia-reperfusion injury (IRI) and improve rat survival after transplantation. Oxygen-dependent autophagy activation was shown to mitigate liver graft's IRI by suppressing endoplasmic reticulum stress response (ERSR), and the NFE2L2-HMOX1 signaling pathway was found to induce autophagy under OCSP.
Article
Biochemistry & Molecular Biology
Mrakic-Sposta Simona, Vezzoli Alessandra, Cova Emanuela, Ticcozzelli Elena, Montorsi Michela, Greco Fulvia, Sepe Vincenzo, Benzoni Ilaria, Meloni Federica, Arbustini Eloisa, Abelli Massimo, Gussoni Maristella
Summary: This study presents a NMR and EPR spectroscopy approach to assess kidney viability and metabolism. The metabolism and oxidative damage of porcine kidney models were examined during organ retrieval, preservation, and transplantation. The results showed higher ROS production and concentration of metabolites in the ex vivo machine perfusion (HMP) group compared to the static cold storage (SCS) group. The HMP group also exhibited better functional recovery after transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Maxime Foguenne, Serena MacMillan, Philipp Kron, Jay Nath, Arnaud Devresse, Martine De Meyer, Mourad Michel, Sarah Hosgood, Tom Darius
Summary: The use of high-risk renal grafts for transplantation requires the optimization of pretransplant assessment and preservation reconditioning strategies. Active oxygenation plays a central role in dynamic preservation strategies to recondition mitochondria and restore cellular energy. The differences between normothermic and hypothermic machine perfusion, as well as the logistic and economic implications, should be considered for implementation at a local level.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Pauline Chazelas, Clara Steichen, Frederic Favreau, Patrick Trouillas, Patrick Hannaert, Raphael Thuillier, Sebastien Giraud, Thierry Hauet, Jerome Guillard
Summary: Oxidative stress plays a crucial role in ischemia reperfusion injury, requiring a comprehensive approach using both chemical and biological tools. New research models, such as stem cell-derived organoids, bridge the gap between in vitro and in vivo studies, providing a deeper understanding of cellular interactions and pathway changes. Additional research paradigms, including molecular modeling and integrative systemic models, offer insights into the dynamic interactions between pathways, aiding in the formulation of integrated predictions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Leonie H. Venema, L. Leonie van Leeuwen, Rene A. Posma, Harry van Goor, Rutger J. Ploeg, Patrick Hannaert, Thierry Hauet, Thomas Minor, Henri G. D. Leuvenink
Summary: This study compared the effectiveness of two blood substitute solutions, one with red blood cells (RBCs) and one without, in kidney evaluation. The results showed that the addition of RBCs reduced renal injury, improved function, and increased renal metabolism. However, a blood-based perfusion solution was still superior.
Article
Engineering, Biomedical
Yannick Le Meur, Eric Delpy, Felix Renard, Thierry Hauet, Lionel Badet, Jean Philippe Rerolle, Antoine Thierry, Matthias Buchler, Franck Zal, Benoit Barrou
Summary: The study evaluated the safety and efficacy of using HEMO(2)life (R) as an additive to preservation solution in renal transplantation, showing lower DGF rates and better renal function in the HEMO(2)life (R) group. Analysis using Kaplan-Meier estimate curves, Cox model, and Bootstrap confirmed the advantages of the HEMO(2)life (R) group.
Article
Biochemistry & Molecular Biology
Pierre-Jean Ferron, Brendan Le Dare, Julie Bronsard, Clara Steichen, Elodie Babina, Romain Pelletier, Thierry Hauet, Isabelle Morel, Karin Tarte, Florian Reizine, Bruno Clement, Bernard Fromenty, Thomas Gicquel
Summary: The study investigated the effects of using hydroxychloroquine (HCQ) to treat COVID-19 patients, especially obese patients, and its impact on drug metabolism and hepatotoxicity. In vitro experiments on HepaRG and RPTEC cells showed that HCQ metabolism was altered in conditions of hepatic steatosis in HepaRG cells, leading to the detection of a new metabolite (carboxychloroquine). Furthermore, HepaRG cells exposed to exogenous fatty acids exhibited increased cytotoxicity of HCQ and enhanced neutral lipid accumulation. These in vitro findings were compared with clinical data from COVID-19 patients treated with HCQ, suggesting that steatosis may increase the risk of altered drug metabolism and potential toxicity of HCQ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, General & Internal
S. Giraud, R. Thuillier, T. Kerforne, B. Barrou, L. Badet, P. Bucur, E. Salame, C. Goumard, E. Savier, J. Branchereau, P. Battistella, O. Mercier, S. Mussot, T. Hauet
Summary: Organ transplantation is the last resort for patients with terminal organ failure. In France, only about 25% of patients on the waiting list can receive a transplant each year. The consideration of new donor populations has led to the need for better management of organ donation and optimization of storage conditions.
BULLETIN DE L ACADEMIE NATIONALE DE MEDECINE
(2022)
Review
Biochemistry & Molecular Biology
Maryne Lepoittevin, Sebastien Giraud, Thomas Kerforne, Benoit Barrou, Lionel Badet, Petru Bucur, Ephrem Salame, Claire Goumard, Eric Savier, Julien Branchereau, Pascal Battistella, Olaf Mercier, Sacha Mussot, Thierry Hauet, Raphael Thuillier
Summary: Organ transplantation is the last resort for treating organ failure, but the shortage of organs remains a challenge. To address this issue, there is a need to expand the donor pool and optimize preservation conditions to improve the quality of transplanted organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Maryne Lepoittevin, Thomas Kerforne, Luc Pellerin, Thierry Hauet, Raphael Thuillier
Summary: The emerging field of molecular predictive medicine is aiming to change the traditional medical approach in renal transplantation by exploring potential biomarker molecules with predictive properties to improve transplantation success. Current research indicates that molecules associated with kidney transplant outcomes mainly relate to inflammation and immune response, as well as other molecular ontologies such as proteasome, growth, and drug metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Valentin Bossard, Nicolas Bourmeyster, Sophie Pasini, Pierre Dupuis, Souleiman El Balkhi, Emmanuel Richard, Hugo Alarcan, Thierry Hauet, Raphael Thuillier
Summary: Due to the increased prevalence of obesity in the world, bariatric surgeries are on the rise and necessitate life-long surveillance for deficiencies. However, inadequate multivitamin supplementation may induce vitamin B6 overload.
Editorial Material
Biochemistry & Molecular Biology
Thierry Hauet, Didier F. Pisani
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Anatomy & Morphology
Beya Robert, Danion Jerome, Vendeuvre Tanguy, Nguyen My-Van, Renault Arthur, Richer Jean-Pierre, Hauet Thierry, Breque Cyril, Faure Jean-Pierre
Summary: This study compared two models of cadaveric dissection to evaluate the importance of the perfused SimLife((R)) technology in identifying the vascular loop in the pelvic region and its dynamic anatomy. The results showed that the perfused model B made it easier to identify and dissect the vascular loop.
SURGICAL AND RADIOLOGIC ANATOMY
(2023)
Article
Cell Biology
Nicolas Melis, Isabelle Rubera, Sebastien Giraud, Marc Cougnon, Christophe Duranton, Mallorie Poet, Gisele Jarretou, Raphael Thuillier, Laurent Counillon, Thierry Hauet, Luc Pellerin, Michel Tauc, Didier F. Pisani
Summary: Through kidney transplantation, ischemia/reperfusion induces tissular injury due to energy shortage, oxidative stress, and endoplasmic reticulum (ER) stress. We found that the specific inhibitor of eukaryotic Initiation Factor 5A (eIF5A) hypusination, GC7, protects kidney cells by modulating unfolded protein response (UPR) pathways. GC7 pretreatment enhances xbp1 splicing and BiP expression while reducing eIF2 alpha phosphorylation and ATF6 nuclear level, indicating a specific modulation of UPR pathways. Inhibition of xbp1 splicing reverses the protective effect of GC7 against anoxia, demonstrating the crucial role of eIF5A hypusination inhibition in UPR modulation.
Editorial Material
Biochemistry & Molecular Biology
Raphael Thuillier
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Immunology
Perrine Burdeyron, Sebastien Giraud, Sonia Brishoual, Estelle Lemarie, Maite Jacquard, Virginie Ameteau, Nadege Boildieu, Hanno Maassen, Tobias M. Huijink, Luc Pellerin, Henri Leuvenink, Thierry Hauet, Clara steichen
